Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis

Detalhes bibliográficos
Autor(a) principal: Knöbl, Terezinha
Data de Publicação: 2008
Outros Autores: Godoy, Silvia Nery, Matushima, Eliana Reiko, Guimarães, Marta Brito, Ferreira, Antonio José Piantino
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/26729
Resumo: A total of eight Escherichia. coli isolates from psittacine birds were serogrouping and investigated for the virulence factors: pili associated with pyelonephritis (pap), S fimbriae (sfa), afimbrial adhesin (afa), capsule K1 (neu), curli fibers (crl, csgA), temperature-sensitive hemagglutinin (tsh), heat labile (LT) and heat stable (STa and STb) enterotoxins, Shiga-like toxins (Stx1 and Stx2), Cytotoxic necrotizing factor (cnf1), haemolysin (hly), aerobactin production (iuc) and serum resistance (iss). The results showed that the isolates belonged to six serogroups: O23; O54; O64; O76; O128 and O152. The found virulence genes were: crl+ in all isolates, pap+, iuc+ and iss+ in three isolates; tsh+ in two isolates. All strains were negative for genes neu, csgA, sfa, afa, hly, cnf and LT, STa, STb, Stx1, Stx2 toxins. Our findings suggested that some E. coli isolated from psittacine birds present some virulence factors of avian pathogenic E. coli (APEC) pathotype.
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spelling Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosisCaracterização molecular dos fatores de virulência de estirpes de Escherichia coli isoladas de papagaios com colibacilose aviáriaPapagaiosEscherichia coliFatores de virulênciaAPECColibaciloseParrotsEscherichia coliVirulence factorsAPECColibacilosisA total of eight Escherichia. coli isolates from psittacine birds were serogrouping and investigated for the virulence factors: pili associated with pyelonephritis (pap), S fimbriae (sfa), afimbrial adhesin (afa), capsule K1 (neu), curli fibers (crl, csgA), temperature-sensitive hemagglutinin (tsh), heat labile (LT) and heat stable (STa and STb) enterotoxins, Shiga-like toxins (Stx1 and Stx2), Cytotoxic necrotizing factor (cnf1), haemolysin (hly), aerobactin production (iuc) and serum resistance (iss). The results showed that the isolates belonged to six serogroups: O23; O54; O64; O76; O128 and O152. The found virulence genes were: crl+ in all isolates, pap+, iuc+ and iss+ in three isolates; tsh+ in two isolates. All strains were negative for genes neu, csgA, sfa, afa, hly, cnf and LT, STa, STb, Stx1, Stx2 toxins. Our findings suggested that some E. coli isolated from psittacine birds present some virulence factors of avian pathogenic E. coli (APEC) pathotype.Oito amostras de Escherichia coli isoladas de papagaios com colibacilose aviária foram sorogrupadas e investigadas para a presença dos fatores de virulência: pili associado a pielonefrite (pap), fímbria S (sfa), adesina afimbrial (afa), cápsula K1 (neu), curli (crl, csgA), hemaglutinina termosensível (tsh), enterotoxinas termo-lábil (LT) e termo-estável (STa e STb), Shiga-like toxinas (Stx1 e Stx2), Fator citotóxico necrotizante (cnf1), hemolisina (hly), aerobactina (iuc) e resistência sérica (iss). Os resultados mostraram que os isolados pertenciam a seis sorogrupos: O23; O54; O64; O76; O128 e O152. Os genes de virulência detectados foram: crl+ em todos os isolados; pap+; iss+ e iuc+ em três isolados, tsh+ em dois isolados. Todas as amostras foram negativas para os genes neu, csgA, sfa, afa, hly, cnf e para as toxinas LT, STa, STb, Stx1 e Stx2. Estes resultados sugerem que amostras de E. coli isoladas de papagaios apresentam alguns fatores de virulência das amostras do patotipo de E. coli patogênica para aves (APEC).Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2008-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2672910.11606/S1413-95962008000700007Brazilian Journal of Veterinary Research and Animal Science; Vol. 45 (2008): Suplemento; 54-60Brazilian Journal of Veterinary Research and Animal Science; Vol. 45 (2008): Suplemento; 54-60Brazilian Journal of Veterinary Research and Animal Science; v. 45 (2008): Suplemento; 54-60Brazilian Journal of Veterinary Research and Animal Science; V. 45 (2008): Suplemento; 54-601678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/26729/28512Knöbl, TerezinhaGodoy, Silvia NeryMatushima, Eliana ReikoGuimarães, Marta BritoFerreira, Antonio José Piantinoinfo:eu-repo/semantics/openAccess2020-06-23T04:12:50Zoai:revistas.usp.br:article/26729Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:00.449074Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis
Caracterização molecular dos fatores de virulência de estirpes de Escherichia coli isoladas de papagaios com colibacilose aviária
title Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis
spellingShingle Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis
Knöbl, Terezinha
Papagaios
Escherichia coli
Fatores de virulência
APEC
Colibacilose
Parrots
Escherichia coli
Virulence factors
APEC
Colibacilosis
title_short Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis
title_full Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis
title_fullStr Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis
title_full_unstemmed Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis
title_sort Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis
author Knöbl, Terezinha
author_facet Knöbl, Terezinha
Godoy, Silvia Nery
Matushima, Eliana Reiko
Guimarães, Marta Brito
Ferreira, Antonio José Piantino
author_role author
author2 Godoy, Silvia Nery
Matushima, Eliana Reiko
Guimarães, Marta Brito
Ferreira, Antonio José Piantino
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Knöbl, Terezinha
Godoy, Silvia Nery
Matushima, Eliana Reiko
Guimarães, Marta Brito
Ferreira, Antonio José Piantino
dc.subject.por.fl_str_mv Papagaios
Escherichia coli
Fatores de virulência
APEC
Colibacilose
Parrots
Escherichia coli
Virulence factors
APEC
Colibacilosis
topic Papagaios
Escherichia coli
Fatores de virulência
APEC
Colibacilose
Parrots
Escherichia coli
Virulence factors
APEC
Colibacilosis
description A total of eight Escherichia. coli isolates from psittacine birds were serogrouping and investigated for the virulence factors: pili associated with pyelonephritis (pap), S fimbriae (sfa), afimbrial adhesin (afa), capsule K1 (neu), curli fibers (crl, csgA), temperature-sensitive hemagglutinin (tsh), heat labile (LT) and heat stable (STa and STb) enterotoxins, Shiga-like toxins (Stx1 and Stx2), Cytotoxic necrotizing factor (cnf1), haemolysin (hly), aerobactin production (iuc) and serum resistance (iss). The results showed that the isolates belonged to six serogroups: O23; O54; O64; O76; O128 and O152. The found virulence genes were: crl+ in all isolates, pap+, iuc+ and iss+ in three isolates; tsh+ in two isolates. All strains were negative for genes neu, csgA, sfa, afa, hly, cnf and LT, STa, STb, Stx1, Stx2 toxins. Our findings suggested that some E. coli isolated from psittacine birds present some virulence factors of avian pathogenic E. coli (APEC) pathotype.
publishDate 2008
dc.date.none.fl_str_mv 2008-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/26729
10.11606/S1413-95962008000700007
url https://www.revistas.usp.br/bjvras/article/view/26729
identifier_str_mv 10.11606/S1413-95962008000700007
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/26729/28512
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 45 (2008): Suplemento; 54-60
Brazilian Journal of Veterinary Research and Animal Science; Vol. 45 (2008): Suplemento; 54-60
Brazilian Journal of Veterinary Research and Animal Science; v. 45 (2008): Suplemento; 54-60
Brazilian Journal of Veterinary Research and Animal Science; V. 45 (2008): Suplemento; 54-60
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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