Influence of Enzootic Bovine Leukosis on phagocytic function of circulating leukocytes from animals with persistent lymphocytosis

• Autor(a) principal: Azedo, Milton Ricardo
• Data de Publicação: 2008
• Outros Autores: Massoco, Cristina de Oliveira, Blagitz, Maiara Garcia, Sanches, Bárbara Gabriela Soares, Souza, Fernando Nogueira de, Batista, Camila Freitas, Sakai, Mônica, Sá-Rocha, Luiz Carlos de, Kfoury Junior, José Roberto, Stricagnolo, Cláudia Regina, Benesi, Fernando José, Libera, Alice Maria Melville Paiva Della
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
• Texto Completo: https://www.revistas.usp.br/bjvras/article/view/26681
• topic: Bovinos; Leucócitos; Fagocitose; Leucose Enzoótica Bovina; Citometria de fluxo; Bovine; Leukocytes; Phagocytosis; Enzootic Bovine Leukosis; Flow Cytometry

This study evaluated the phagocytosis of propidium iodide-labeled Staphylococcus aureus (PI-Sa) by circulating leukocytes obtained from five Enzootic Bovine Leukosis (EBL)-negative cows, five naturally EBL-infected, non-lymphocytotic cows, and five EBL-positive cows with persistent lymphocytosis (PL), analyzed by flow cytometry. Among cells obtained from EBL-infected cows, presenting PL, the percentage of leukocytes carrying out phagocytosis (7.20%), was smaller (p=0.047) than that verified among cells obtained from non-infected (12.90%), and from BLV-infected, non-lymphocytotic cows (14.70%). Furthermore, leukocytes obtained from EBL-infected cows, presenting PL, showed smaller phagocytosis intensity (characterized by the intensity of propidium iodide fluorescence) than leukocytes obtained from non-infected and from EBL-infected, non-lymphocytotic, cows (p<0.001). Therefore, results show a decreased phagocytic function among circulating leukocytes obtained from BLV-infected, lymphocytotic cows.