Experimental infection of vampire bats Desmodus rotundus (E. Geoffroy) maintained in captivity by feeding defibrinated blood added with rabies virus
Main Author: | |
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Publication Date: | 2009 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Brazilian Journal of Veterinary Research and Animal Science |
Download full: | https://www.revistas.usp.br/bjvras/article/view/26754 |
Summary: | In vampire bats, food sharing behavior would contribute for the oral transmission of rabies virus among the roostmates. To test this hypothesis, 10 captive Desmodus rotundus bats were fed defibrinated swine blood containing mice brain suspension of PV-strain of rabies virus. Other 10 bats were fed blood mixed with a mice brain suspension of T-9/95 vampire-bat-field isolate of rabies virus. Another group of 10 bats was inoculated intramuscularly with a mice brain suspension of the T-9/95 isolate. Other 20 bats were maintained without treatment and fed defibrinated swine blood for 158 days. All animals found dead during the observation period or those sacrificed at the end of the experiment were necropsied and specimens such as the brain and non-nervous tissues were collected for rabies examination. Four bats inoculated intramuscularly developed clinical rabies, with signs lasting 1-2 days, and the survival periods ranged from 11-14 days. The initial rabies diagnosis was based on direct fluorescent antibody (dFA) and mouse inoculation test (MIT) performed only on brain specimens, and subsequently, brains and the non-nervous materials were further reexamined by means of dFA, MIT and heminested-polymerase chain reaction (ht-PCR) technique. The intake of the PV-strain caused rabies in 2 bats, with survival period of 25 and 32 days, while the three bats ingesting the T-9/95 isolate presented periods of 26-31 days. Although discrepant results were found among the diagnostic tests, viruses have disseminated to the central nervous system and other organs, as seen in bats inoculated intramuscularly. |
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oai:revistas.usp.br:article/26754 |
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USP-49 |
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Brazilian Journal of Veterinary Research and Animal Science |
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https://www.revistas.usp.br/bjvras/index |
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Experimental infection of vampire bats Desmodus rotundus (E. Geoffroy) maintained in captivity by feeding defibrinated blood added with rabies virusInfecção experimental de morcegos hematófagos Desmodus rotundus (E. Geoffroy) mantidos em cativeiro e alimentados com sangue desfibrinado adicionado de vírus da raivaVírus da raivaInoculação oralSangueChiropteraRabies virusOral inoculationBloodChiropteraIn vampire bats, food sharing behavior would contribute for the oral transmission of rabies virus among the roostmates. To test this hypothesis, 10 captive Desmodus rotundus bats were fed defibrinated swine blood containing mice brain suspension of PV-strain of rabies virus. Other 10 bats were fed blood mixed with a mice brain suspension of T-9/95 vampire-bat-field isolate of rabies virus. Another group of 10 bats was inoculated intramuscularly with a mice brain suspension of the T-9/95 isolate. Other 20 bats were maintained without treatment and fed defibrinated swine blood for 158 days. All animals found dead during the observation period or those sacrificed at the end of the experiment were necropsied and specimens such as the brain and non-nervous tissues were collected for rabies examination. Four bats inoculated intramuscularly developed clinical rabies, with signs lasting 1-2 days, and the survival periods ranged from 11-14 days. The initial rabies diagnosis was based on direct fluorescent antibody (dFA) and mouse inoculation test (MIT) performed only on brain specimens, and subsequently, brains and the non-nervous materials were further reexamined by means of dFA, MIT and heminested-polymerase chain reaction (ht-PCR) technique. The intake of the PV-strain caused rabies in 2 bats, with survival period of 25 and 32 days, while the three bats ingesting the T-9/95 isolate presented periods of 26-31 days. Although discrepant results were found among the diagnostic tests, viruses have disseminated to the central nervous system and other organs, as seen in bats inoculated intramuscularly.Em morcegos hematófagos, o hábito de compartilhar alimento poderia contribuir na transmissão oral do vírus da raiva. Para verificar esta hipótese, 10 morcegos Desmodus rotundus em cativeiro foram alimentados com sangue suíno desfibrinado, contendo suspensão de cérebros de camundongos infectados com vírus rábico PV. Outros 10 camundongos receberam sangue contendo suspensão cerebral de camundongos infectados com vírus de morcego hematófago (T-9/ 95). Um grupo de 10 camundongos foi inoculado intramuscularmente com suspensão de vírus T-9/95. Outros 20 morcegos foram mantidos sem tratamento e alimentados com sangue desfibrinado por 158 dias. Todos os animais encontrados mortos durante o período de observação ou sacrificados no final do experimento foram necropsiados e os cérebros e órgãos não-nervosos foram colhidos para a confirmação da raiva. Quatro morcegos inoculados intramuscularmente apresentaram raiva clínica, com sinais persistindo por 1-2 dias e os períodos de sobrevivência variaram de 11-14 dias. O diagnóstico da raiva inicialmente foi realizado somente com os fragmentos do cérebro, submetendo-os às provas de imunoflurescência direta (IFD) e inoculação em camundongos (IC). Subseqüentemente, os cérebros e os órgãos não-nervosos foram reexaminados com as técnicas de IFD, IC e heminested-polymerase chain reaction (ht-PCR). A ingestão do vírus PV causou raiva em dois morcegos, com período de sobrevivência de 25 e 32 dias, enquanto que os três morcegos que ingeriram o isolado T-9/95 apresentaram períodos de 26-31 dias. Embora encontrando resultados discrepantes entre as técnicas diagnósticas utilizadas, os vírus ingeridos pelos morcegos foram detectados no sistema nervoso central e outros órgãos não-nervosos, como nos morcegos inoculados intramuscularmente.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2009-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2675410.11606/issn.1678-4456.bjvras.2009.26754Brazilian Journal of Veterinary Research and Animal Science; Vol. 46 Núm. 2 (2009); 92-100 Brazilian Journal of Veterinary Research and Animal Science; Vol. 46 No. 2 (2009); 92-100 Brazilian Journal of Veterinary Research and Animal Science; v. 46 n. 2 (2009); 92-100 Brazilian Journal of Veterinary Research and Animal Science; V. 46 N. 2 (2009); 92-100 1678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/26754/28537Souza, Maria Conceição Aparecida MacedoNassar, Alessandra Figueiredo de CastroCortez, AdrianaSakai, TakeoItou, TakuyaCunha, Elenice Maria SequetinRichtzenhain, Leonardo JoséIto, Fumio Honmainfo:eu-repo/semantics/openAccess2020-06-23T04:12:05Zoai:revistas.usp.br:article/26754Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:01.992039Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Experimental infection of vampire bats Desmodus rotundus (E. Geoffroy) maintained in captivity by feeding defibrinated blood added with rabies virus Infecção experimental de morcegos hematófagos Desmodus rotundus (E. Geoffroy) mantidos em cativeiro e alimentados com sangue desfibrinado adicionado de vírus da raiva |
title |
Experimental infection of vampire bats Desmodus rotundus (E. Geoffroy) maintained in captivity by feeding defibrinated blood added with rabies virus |
spellingShingle |
Experimental infection of vampire bats Desmodus rotundus (E. Geoffroy) maintained in captivity by feeding defibrinated blood added with rabies virus Souza, Maria Conceição Aparecida Macedo Vírus da raiva Inoculação oral Sangue Chiroptera Rabies virus Oral inoculation Blood Chiroptera |
title_short |
Experimental infection of vampire bats Desmodus rotundus (E. Geoffroy) maintained in captivity by feeding defibrinated blood added with rabies virus |
title_full |
Experimental infection of vampire bats Desmodus rotundus (E. Geoffroy) maintained in captivity by feeding defibrinated blood added with rabies virus |
title_fullStr |
Experimental infection of vampire bats Desmodus rotundus (E. Geoffroy) maintained in captivity by feeding defibrinated blood added with rabies virus |
title_full_unstemmed |
Experimental infection of vampire bats Desmodus rotundus (E. Geoffroy) maintained in captivity by feeding defibrinated blood added with rabies virus |
title_sort |
Experimental infection of vampire bats Desmodus rotundus (E. Geoffroy) maintained in captivity by feeding defibrinated blood added with rabies virus |
author |
Souza, Maria Conceição Aparecida Macedo |
author_facet |
Souza, Maria Conceição Aparecida Macedo Nassar, Alessandra Figueiredo de Castro Cortez, Adriana Sakai, Takeo Itou, Takuya Cunha, Elenice Maria Sequetin Richtzenhain, Leonardo José Ito, Fumio Honma |
author_role |
author |
author2 |
Nassar, Alessandra Figueiredo de Castro Cortez, Adriana Sakai, Takeo Itou, Takuya Cunha, Elenice Maria Sequetin Richtzenhain, Leonardo José Ito, Fumio Honma |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Souza, Maria Conceição Aparecida Macedo Nassar, Alessandra Figueiredo de Castro Cortez, Adriana Sakai, Takeo Itou, Takuya Cunha, Elenice Maria Sequetin Richtzenhain, Leonardo José Ito, Fumio Honma |
dc.subject.por.fl_str_mv |
Vírus da raiva Inoculação oral Sangue Chiroptera Rabies virus Oral inoculation Blood Chiroptera |
topic |
Vírus da raiva Inoculação oral Sangue Chiroptera Rabies virus Oral inoculation Blood Chiroptera |
description |
In vampire bats, food sharing behavior would contribute for the oral transmission of rabies virus among the roostmates. To test this hypothesis, 10 captive Desmodus rotundus bats were fed defibrinated swine blood containing mice brain suspension of PV-strain of rabies virus. Other 10 bats were fed blood mixed with a mice brain suspension of T-9/95 vampire-bat-field isolate of rabies virus. Another group of 10 bats was inoculated intramuscularly with a mice brain suspension of the T-9/95 isolate. Other 20 bats were maintained without treatment and fed defibrinated swine blood for 158 days. All animals found dead during the observation period or those sacrificed at the end of the experiment were necropsied and specimens such as the brain and non-nervous tissues were collected for rabies examination. Four bats inoculated intramuscularly developed clinical rabies, with signs lasting 1-2 days, and the survival periods ranged from 11-14 days. The initial rabies diagnosis was based on direct fluorescent antibody (dFA) and mouse inoculation test (MIT) performed only on brain specimens, and subsequently, brains and the non-nervous materials were further reexamined by means of dFA, MIT and heminested-polymerase chain reaction (ht-PCR) technique. The intake of the PV-strain caused rabies in 2 bats, with survival period of 25 and 32 days, while the three bats ingesting the T-9/95 isolate presented periods of 26-31 days. Although discrepant results were found among the diagnostic tests, viruses have disseminated to the central nervous system and other organs, as seen in bats inoculated intramuscularly. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26754 10.11606/issn.1678-4456.bjvras.2009.26754 |
url |
https://www.revistas.usp.br/bjvras/article/view/26754 |
identifier_str_mv |
10.11606/issn.1678-4456.bjvras.2009.26754 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26754/28537 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 46 Núm. 2 (2009); 92-100 Brazilian Journal of Veterinary Research and Animal Science; Vol. 46 No. 2 (2009); 92-100 Brazilian Journal of Veterinary Research and Animal Science; v. 46 n. 2 (2009); 92-100 Brazilian Journal of Veterinary Research and Animal Science; V. 46 N. 2 (2009); 92-100 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051559301873664 |