Vesicular Stomatitis: Serological Survey in beef cattle from region of Araçatuba, São Paulo State, Brazil in 2000
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/11388 |
Resumo: | In 2000 a total of 1099 serum samples were obtained of adult beef cattle, from 6 herds located at Auriflama, Gastão Vidigal,, Magda, Santo Antônio do Aracanguá, Sebastianópolis do Sul e Turiúba, counties from the region of Araçatuba. Sera were tested for neutralizing antibodies to vesicular stomatitis virus by microtiter neutralization assay, using the virus strain Indiana 1 Costa Rica/72 with 10(6,0) DICT50/50mL. The indicator cells were African green monkey kidney (VERO-CCL 81), purchased from American Type Cell Colection (ATCC). The neutralization titers of each serum were the reciprocal of the highest dilution expressed at log10 that protected 100.00% of the cell monolayer. Sera with values above 1,6 log10 (1/40) were considered positive for Vesicular Stomatitis virus. Twenty-eight (2.50%) out of 1.099 samples were positive for Vesicular Stomatitis virus Indiana 1, with antibodies titers ranging from 1.9 to 2.8. Except in Turiuba, in the others 5 herds there were positive animals. The presente survey showed the presence the Vesicular Stomatitis virus in the herds stimulating the humoral response. More studies are necessary to verify the site of persistence of the virus. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Vesicular Stomatitis: Serological Survey in beef cattle from region of Araçatuba, São Paulo State, Brazil in 2000Pesquisa de anticorpos contra o vírus da Estomatite Vesicular em bovinos de corte criados na região de Araçatuba, Estado de São Paulo, Brasil em 2000Estomatite vesicular animalAnticorposBovinos de corteVesicular StomatitisAntibodiesBeef cattleIn 2000 a total of 1099 serum samples were obtained of adult beef cattle, from 6 herds located at Auriflama, Gastão Vidigal,, Magda, Santo Antônio do Aracanguá, Sebastianópolis do Sul e Turiúba, counties from the region of Araçatuba. Sera were tested for neutralizing antibodies to vesicular stomatitis virus by microtiter neutralization assay, using the virus strain Indiana 1 Costa Rica/72 with 10(6,0) DICT50/50mL. The indicator cells were African green monkey kidney (VERO-CCL 81), purchased from American Type Cell Colection (ATCC). The neutralization titers of each serum were the reciprocal of the highest dilution expressed at log10 that protected 100.00% of the cell monolayer. Sera with values above 1,6 log10 (1/40) were considered positive for Vesicular Stomatitis virus. Twenty-eight (2.50%) out of 1.099 samples were positive for Vesicular Stomatitis virus Indiana 1, with antibodies titers ranging from 1.9 to 2.8. Except in Turiuba, in the others 5 herds there were positive animals. The presente survey showed the presence the Vesicular Stomatitis virus in the herds stimulating the humoral response. More studies are necessary to verify the site of persistence of the virus.No ano de 2000 foram pesquisadas 1099 amostras de soro de bovinos de corte, adultos, pertencentes à seis rebanhos situados nos municípios de Auriflama, Gastão Vidigal,, Magda, Santo Antônio do Aracanguá, Sebastianópolis do Sul e Turiúba, região de Araçatuba. A técnica empregada para a dosagem de anticorpos para o vírus da Estomatite Vesicular foi a soroneutralização em microplacas, utilizando a cepa Indiana 1 Costa Rica/72 com título de 10(6,0) DICT50/50mL. As células utilizadas foram as da linhagem de Rim de Macaco Verde Africano (VERO-CCL 81), provenientes do American Type Cell Colection (ATCC). O título de anticorpos de cada amostra de soro, foi calculado como a recíproca da maior diluição do soro expresso em log10, que protegeu 100,00% da monocamada celular. Foram consideradas positivas as amostras com título superior a 1,6 log10 (1/40). Das 1099 amostras de soro analisadas, 28 (2,50%) foram positivas ao vírus da Estomatite Vesicular tipo Indiana 1, com títulos de anticorpos variando de 1,9 à 2,8. Com exceção do rebanho situado no município de Turiúba, nos outros cinco foram encontrados animais reagentes ao vírus. Esta avaliação mostrou que houve circulação do vírus da Estomatite Vesicular nestes rebanhos provocando resposta humoral. Sendo assim é necessário a realização de estudos para se determinar o sítio de permanência do vírus.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2003-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/1138810.1590/S1413-95962003000100003Brazilian Journal of Veterinary Research and Animal Science; Vol. 40 Núm. 1 (2003); 29-35Brazilian Journal of Veterinary Research and Animal Science; Vol. 40 No. 1 (2003); 29-35Brazilian Journal of Veterinary Research and Animal Science; v. 40 n. 1 (2003); 29-35Brazilian Journal of Veterinary Research and Animal Science; V. 40 N. 1 (2003); 29-351678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/11388/13156De Stefano, ElianaAraújo, Wanderley Pereira dePassos, Estevão de CamargoPituco, Edviges Maristelainfo:eu-repo/semantics/openAccess2020-06-23T04:34:20Zoai:revistas.usp.br:article/11388Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:40.138204Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Vesicular Stomatitis: Serological Survey in beef cattle from region of Araçatuba, São Paulo State, Brazil in 2000 Pesquisa de anticorpos contra o vírus da Estomatite Vesicular em bovinos de corte criados na região de Araçatuba, Estado de São Paulo, Brasil em 2000 |
title |
Vesicular Stomatitis: Serological Survey in beef cattle from region of Araçatuba, São Paulo State, Brazil in 2000 |
spellingShingle |
Vesicular Stomatitis: Serological Survey in beef cattle from region of Araçatuba, São Paulo State, Brazil in 2000 De Stefano, Eliana Estomatite vesicular animal Anticorpos Bovinos de corte Vesicular Stomatitis Antibodies Beef cattle |
title_short |
Vesicular Stomatitis: Serological Survey in beef cattle from region of Araçatuba, São Paulo State, Brazil in 2000 |
title_full |
Vesicular Stomatitis: Serological Survey in beef cattle from region of Araçatuba, São Paulo State, Brazil in 2000 |
title_fullStr |
Vesicular Stomatitis: Serological Survey in beef cattle from region of Araçatuba, São Paulo State, Brazil in 2000 |
title_full_unstemmed |
Vesicular Stomatitis: Serological Survey in beef cattle from region of Araçatuba, São Paulo State, Brazil in 2000 |
title_sort |
Vesicular Stomatitis: Serological Survey in beef cattle from region of Araçatuba, São Paulo State, Brazil in 2000 |
author |
De Stefano, Eliana |
author_facet |
De Stefano, Eliana Araújo, Wanderley Pereira de Passos, Estevão de Camargo Pituco, Edviges Maristela |
author_role |
author |
author2 |
Araújo, Wanderley Pereira de Passos, Estevão de Camargo Pituco, Edviges Maristela |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
De Stefano, Eliana Araújo, Wanderley Pereira de Passos, Estevão de Camargo Pituco, Edviges Maristela |
dc.subject.por.fl_str_mv |
Estomatite vesicular animal Anticorpos Bovinos de corte Vesicular Stomatitis Antibodies Beef cattle |
topic |
Estomatite vesicular animal Anticorpos Bovinos de corte Vesicular Stomatitis Antibodies Beef cattle |
description |
In 2000 a total of 1099 serum samples were obtained of adult beef cattle, from 6 herds located at Auriflama, Gastão Vidigal,, Magda, Santo Antônio do Aracanguá, Sebastianópolis do Sul e Turiúba, counties from the region of Araçatuba. Sera were tested for neutralizing antibodies to vesicular stomatitis virus by microtiter neutralization assay, using the virus strain Indiana 1 Costa Rica/72 with 10(6,0) DICT50/50mL. The indicator cells were African green monkey kidney (VERO-CCL 81), purchased from American Type Cell Colection (ATCC). The neutralization titers of each serum were the reciprocal of the highest dilution expressed at log10 that protected 100.00% of the cell monolayer. Sera with values above 1,6 log10 (1/40) were considered positive for Vesicular Stomatitis virus. Twenty-eight (2.50%) out of 1.099 samples were positive for Vesicular Stomatitis virus Indiana 1, with antibodies titers ranging from 1.9 to 2.8. Except in Turiuba, in the others 5 herds there were positive animals. The presente survey showed the presence the Vesicular Stomatitis virus in the herds stimulating the humoral response. More studies are necessary to verify the site of persistence of the virus. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/11388 10.1590/S1413-95962003000100003 |
url |
https://www.revistas.usp.br/bjvras/article/view/11388 |
identifier_str_mv |
10.1590/S1413-95962003000100003 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/11388/13156 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 40 Núm. 1 (2003); 29-35 Brazilian Journal of Veterinary Research and Animal Science; Vol. 40 No. 1 (2003); 29-35 Brazilian Journal of Veterinary Research and Animal Science; v. 40 n. 1 (2003); 29-35 Brazilian Journal of Veterinary Research and Animal Science; V. 40 N. 1 (2003); 29-35 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
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1797051557125029888 |