In vitro development of bovine embryos cultured under different fetal calf serum concentrations and cell types
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/34374 |
Resumo: | The present work was carried out to evaluate the in vitro development of bovine embryo co-cultured in granulosa, oviduct, BRL or VERO cells co-cultures, supplemented with 5% or 10% of Fetal Calf Serum (FCS). Cummulus oocyte complexes were aspirated, matured and fertilized in vitro. Embryonic structures were divided into eight treatments. They were placed in culture media TCM 199 containing granulosa, oviduct, BRL or VERO cells, each of them added with 5% or 10% FCS. The conditions for the co-culture were 38.5 ºC, 5% CO2 in air and high humidity for ten consecutive days. Cleavage, blastocyst and hatching rates did not differ (p > 0.05) in co-culture with primary cells (granulosa and oviduct) when FCS concentration increased from 5 to 10%. However, in continuous cells co-culture (BRL and VERO), when FCS concentration increased from 5% to 10%, the blastocyst development rate decreased significantly (p < 0.05) from 33.6 to 16.3% and from 40 to 16.5% in embryo co-culture with BRL and VERO cells, respectively. |
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Brazilian Journal of Veterinary Research and Animal Science |
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In vitro development of bovine embryos cultured under different fetal calf serum concentrations and cell typesDesenvolvimento in vitro de embriões bovinos cultivados sob diferentes concentrações de soro fetal bovino e tipos celularesCo-cultivoBlastocistoGranulosaOvidutoVeroBRLCo-cultureBlastocystGranulosaOviductVeroBRLThe present work was carried out to evaluate the in vitro development of bovine embryo co-cultured in granulosa, oviduct, BRL or VERO cells co-cultures, supplemented with 5% or 10% of Fetal Calf Serum (FCS). Cummulus oocyte complexes were aspirated, matured and fertilized in vitro. Embryonic structures were divided into eight treatments. They were placed in culture media TCM 199 containing granulosa, oviduct, BRL or VERO cells, each of them added with 5% or 10% FCS. The conditions for the co-culture were 38.5 ºC, 5% CO2 in air and high humidity for ten consecutive days. Cleavage, blastocyst and hatching rates did not differ (p > 0.05) in co-culture with primary cells (granulosa and oviduct) when FCS concentration increased from 5 to 10%. However, in continuous cells co-culture (BRL and VERO), when FCS concentration increased from 5% to 10%, the blastocyst development rate decreased significantly (p < 0.05) from 33.6 to 16.3% and from 40 to 16.5% in embryo co-culture with BRL and VERO cells, respectively.O presente trabalho foi realizado com o objetivo de avaliar o desenvolvimento in vitro de embriões bovinos co-cultivados em células da granulosa, do oviduto, BRL e VERO, suplementados com 5% ou 10% de Soro Fetal Bovino (SFB). Os complexos cummulus oócitos foram aspirados, maturados e fecundados in vitro. As estruturas embrionárias foram divididas em oito tratamentos: co-cultivo em TCM 199 contendo células da granulosa, do oviduto, BRL ou VERO adicionadas com 5% ou 10% de SFB. As condições de cultivo foram 38.5 ºC, 5% CO2 em ar e alta humidade por dez dias consecutivos. Os índices de clivagem, blastocisto e eclosão não diferiram (p > 0,05) no co-cultivo com células primárias (granulosa e oviduto) quando a concentração de SFB aumentou de 5 para 10%. Entretanto, no co-cultivo com células de linhagens contínuas (BRL e VERO), quando a concentração de SFB aumentou de 5% para 10%, os índices de blastocistos diminuíram significativamente (p < 0,05) de 33,6 para 16,3 % e de 40 para 16,5% nos embriões bovinos co-cultivados com células VERO e BRL, respectivamente.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2011-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/3437410.11606/S1413-95962011000100005Brazilian Journal of Veterinary Research and Animal Science; Vol. 48 Núm. 1 (2011); 38-45 Brazilian Journal of Veterinary Research and Animal Science; Vol. 48 No. 1 (2011); 38-45 Brazilian Journal of Veterinary Research and Animal Science; v. 48 n. 1 (2011); 38-45 Brazilian Journal of Veterinary Research and Animal Science; V. 48 N. 1 (2011); 38-45 1678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/34374/37112Tavares, Lilian Mara TrevisanFeitosa, Weber BeringuiOliveira, Viviani Purri deNichi, MarcilioAssumpção, Mayra Elena Ortiz D'ÁvilaVisintin, José Antonioinfo:eu-repo/semantics/openAccess2020-06-23T04:09:52Zoai:revistas.usp.br:article/34374Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:10.019813Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
In vitro development of bovine embryos cultured under different fetal calf serum concentrations and cell types Desenvolvimento in vitro de embriões bovinos cultivados sob diferentes concentrações de soro fetal bovino e tipos celulares |
title |
In vitro development of bovine embryos cultured under different fetal calf serum concentrations and cell types |
spellingShingle |
In vitro development of bovine embryos cultured under different fetal calf serum concentrations and cell types Tavares, Lilian Mara Trevisan Co-cultivo Blastocisto Granulosa Oviduto Vero BRL Co-culture Blastocyst Granulosa Oviduct Vero BRL |
title_short |
In vitro development of bovine embryos cultured under different fetal calf serum concentrations and cell types |
title_full |
In vitro development of bovine embryos cultured under different fetal calf serum concentrations and cell types |
title_fullStr |
In vitro development of bovine embryos cultured under different fetal calf serum concentrations and cell types |
title_full_unstemmed |
In vitro development of bovine embryos cultured under different fetal calf serum concentrations and cell types |
title_sort |
In vitro development of bovine embryos cultured under different fetal calf serum concentrations and cell types |
author |
Tavares, Lilian Mara Trevisan |
author_facet |
Tavares, Lilian Mara Trevisan Feitosa, Weber Beringui Oliveira, Viviani Purri de Nichi, Marcilio Assumpção, Mayra Elena Ortiz D'Ávila Visintin, José Antonio |
author_role |
author |
author2 |
Feitosa, Weber Beringui Oliveira, Viviani Purri de Nichi, Marcilio Assumpção, Mayra Elena Ortiz D'Ávila Visintin, José Antonio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Tavares, Lilian Mara Trevisan Feitosa, Weber Beringui Oliveira, Viviani Purri de Nichi, Marcilio Assumpção, Mayra Elena Ortiz D'Ávila Visintin, José Antonio |
dc.subject.por.fl_str_mv |
Co-cultivo Blastocisto Granulosa Oviduto Vero BRL Co-culture Blastocyst Granulosa Oviduct Vero BRL |
topic |
Co-cultivo Blastocisto Granulosa Oviduto Vero BRL Co-culture Blastocyst Granulosa Oviduct Vero BRL |
description |
The present work was carried out to evaluate the in vitro development of bovine embryo co-cultured in granulosa, oviduct, BRL or VERO cells co-cultures, supplemented with 5% or 10% of Fetal Calf Serum (FCS). Cummulus oocyte complexes were aspirated, matured and fertilized in vitro. Embryonic structures were divided into eight treatments. They were placed in culture media TCM 199 containing granulosa, oviduct, BRL or VERO cells, each of them added with 5% or 10% FCS. The conditions for the co-culture were 38.5 ºC, 5% CO2 in air and high humidity for ten consecutive days. Cleavage, blastocyst and hatching rates did not differ (p > 0.05) in co-culture with primary cells (granulosa and oviduct) when FCS concentration increased from 5 to 10%. However, in continuous cells co-culture (BRL and VERO), when FCS concentration increased from 5% to 10%, the blastocyst development rate decreased significantly (p < 0.05) from 33.6 to 16.3% and from 40 to 16.5% in embryo co-culture with BRL and VERO cells, respectively. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/34374 10.11606/S1413-95962011000100005 |
url |
https://www.revistas.usp.br/bjvras/article/view/34374 |
identifier_str_mv |
10.11606/S1413-95962011000100005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/34374/37112 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 48 Núm. 1 (2011); 38-45 Brazilian Journal of Veterinary Research and Animal Science; Vol. 48 No. 1 (2011); 38-45 Brazilian Journal of Veterinary Research and Animal Science; v. 48 n. 1 (2011); 38-45 Brazilian Journal of Veterinary Research and Animal Science; V. 48 N. 1 (2011); 38-45 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051560005468160 |