Capacitação espermática de sêmem congelado de onça pintada (Panthera onca) e ensaio de penetração em oócitos de hamster livres de zona pelúcida

• Autor(a) principal: Paz, Regina Celia Rodrigues da
• Data de Publicação: 2007
• Outros Autores: Züge, Roberta Mara, Barnabe, Valquíria Hyppólito
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
• Texto Completo: https://www.revistas.usp.br/bjvras/article/view/26616
• topic: Panthera onca; Ensaio de penetração; Capacitação espermática; Criopreservação de sêmen; Panthera onca; Penetration assay; Sperm capacitation; Sperm cryopreservation

Assisted reproductive technologies in endangered species, such as artificial insemination, in vitro fertilization and embryo transfer, can be viewed as one potential approach for safeguarding species. Toward this aim, the objective of this study was to evaluate the fertility of frozen jaguar (Panthera onca) sperm and Tyrod's Talp PVA capacitation medium using the hamster zona free oocyte penetration assay. Ejaculates were collected from nine animals using electroejaculation and cryopreserved. Sperm capacitation was performed by swim-up technique using Tyrod's Talp PVA medium at room temperature. Penetration was considered when the spermatozoa head decondensation was visualized within the oocyte. This assay showed 15.4% penetrations (350/2275 oocytes). Results of this study showed high sperm abnormalities, low sperm quality after cryopreservation, and low percentage of penetrations. However, the penetration results showed that the cryopreserved jaguar's semen can be used for artificial insemination, in vitro fertilization and intra cytoplasmatic sperm injection, supporting the semen bank creation for this specie.