Occurrence of anti-Toxoplasma gondii antibodies in caprines from Pitanga City, Paraná State, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/26619 |
Resumo: | The aim of this study was to verify the prevalence of anti-Toxoplasma gondii antibodies in sera from naturally infected milk-producing caprines using indirect immunofluorescence assay (IFA) and modified agglutination test (MAT), determine specificity and sensibility of MAT and to evaluate the agreement of results between the tests. For this study, 282 caprine serum samples were collected from farms in the city of Pitanga, Paraná State, a region with a high pecuary of small ruminants. Prevalence found by IFA was of 44.68% and 23.05% by MAT. There was statistically significant difference (p=0.0086) in relation to the gender of animals, being females more seropositive. Sensitivity and specificity results for MAT were, respectively, 42.2% and 94.9%. By comparing results, a moderate agreement was observed (Kappa = 0.42). The high prevalence for the infection, associated to high titers found by IFA and clinical signs presented in the herds suggest toxoplasmosis as one of the causes of reproductive problems in caprines. Sensitivity and specificity results for MAT obtained in this study allow this test to be recommended as a confirmatory test for the caprine species. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Occurrence of anti-Toxoplasma gondii antibodies in caprines from Pitanga City, Paraná State, BrazilOcorrência de anticorpos anti-Toxoplasma gondii em caprinos de Pitanga, Paraná, BrasilToxoplasma gondiiImunofluorescência indiretaTeste de aglutinação modificadaCaprinosProblemas reprodutivosToxoplasma gondiiIndirect immunofluorescenceModified agglutination testCaprinesReproductive problemsThe aim of this study was to verify the prevalence of anti-Toxoplasma gondii antibodies in sera from naturally infected milk-producing caprines using indirect immunofluorescence assay (IFA) and modified agglutination test (MAT), determine specificity and sensibility of MAT and to evaluate the agreement of results between the tests. For this study, 282 caprine serum samples were collected from farms in the city of Pitanga, Paraná State, a region with a high pecuary of small ruminants. Prevalence found by IFA was of 44.68% and 23.05% by MAT. There was statistically significant difference (p=0.0086) in relation to the gender of animals, being females more seropositive. Sensitivity and specificity results for MAT were, respectively, 42.2% and 94.9%. By comparing results, a moderate agreement was observed (Kappa = 0.42). The high prevalence for the infection, associated to high titers found by IFA and clinical signs presented in the herds suggest toxoplasmosis as one of the causes of reproductive problems in caprines. Sensitivity and specificity results for MAT obtained in this study allow this test to be recommended as a confirmatory test for the caprine species.Objetivou-se verificar a prevalência de anticorpos anti-Toxoplasma gondii em soros de caprinos de produção leiteira naturalmente infectados utilizando os testes de imunofluorescência indireta (IFI) e o de aglutinação modificada (MAT), determinar a especificidade e a sensibilidade do MAT e avaliar a concordância dos resultados entre os testes. Para o estudo foram coletadas 282 amostras de soro caprino, de propriedades do município de Pitanga, Paraná, região com uma grande pecuária de pequenos ruminantes. A prevalência encontrada pela IFI foi de 44,68% e 23,05% pelo MAT. Houve diferença estatisticamente significativa (p=0,0086) em relação ao sexo dos animais, sendo que as fêmeas foram mais soropositivas. Os resultados de sensibilidade e especificidade para o MAT foram, respectivamente, 42,2% e 94,9%. Na comparação dos resultados foi observada uma concordância moderada (Kappa = 0,42). A alta prevalência da infecção, aliada aos altos títulos obtidos pela IFI e aos sinais clínicos presentes nos rebanhos sugere a toxoplasmose como uma possível causa de problemas reprodutivos em caprinos. Os resultados de sensibilidade e especificidade para o MAT obtidos neste estudo permitem recomendá-lo como teste confirmatório para a espécie caprina.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2007-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2661910.11606/issn.1678-4456.bjvras.2007.26619Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 Núm. 5 (2007); 358-363Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 No. 5 (2007); 358-363Brazilian Journal of Veterinary Research and Animal Science; v. 44 n. 5 (2007); 358-363Brazilian Journal of Veterinary Research and Animal Science; V. 44 N. 5 (2007); 358-3631678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/26619/28402Reis, Célia Rosimarie dosLopes, Fabiana Maria RuizGonçalves, Daniela DibFreire, Roberta LemosGarcia, João LuisNavarro, Italmar Teodoricoinfo:eu-repo/semantics/openAccess2020-06-23T04:14:44Zoai:revistas.usp.br:article/26619Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:53.962872Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Occurrence of anti-Toxoplasma gondii antibodies in caprines from Pitanga City, Paraná State, Brazil Ocorrência de anticorpos anti-Toxoplasma gondii em caprinos de Pitanga, Paraná, Brasil |
title |
Occurrence of anti-Toxoplasma gondii antibodies in caprines from Pitanga City, Paraná State, Brazil |
spellingShingle |
Occurrence of anti-Toxoplasma gondii antibodies in caprines from Pitanga City, Paraná State, Brazil Reis, Célia Rosimarie dos Toxoplasma gondii Imunofluorescência indireta Teste de aglutinação modificada Caprinos Problemas reprodutivos Toxoplasma gondii Indirect immunofluorescence Modified agglutination test Caprines Reproductive problems |
title_short |
Occurrence of anti-Toxoplasma gondii antibodies in caprines from Pitanga City, Paraná State, Brazil |
title_full |
Occurrence of anti-Toxoplasma gondii antibodies in caprines from Pitanga City, Paraná State, Brazil |
title_fullStr |
Occurrence of anti-Toxoplasma gondii antibodies in caprines from Pitanga City, Paraná State, Brazil |
title_full_unstemmed |
Occurrence of anti-Toxoplasma gondii antibodies in caprines from Pitanga City, Paraná State, Brazil |
title_sort |
Occurrence of anti-Toxoplasma gondii antibodies in caprines from Pitanga City, Paraná State, Brazil |
author |
Reis, Célia Rosimarie dos |
author_facet |
Reis, Célia Rosimarie dos Lopes, Fabiana Maria Ruiz Gonçalves, Daniela Dib Freire, Roberta Lemos Garcia, João Luis Navarro, Italmar Teodorico |
author_role |
author |
author2 |
Lopes, Fabiana Maria Ruiz Gonçalves, Daniela Dib Freire, Roberta Lemos Garcia, João Luis Navarro, Italmar Teodorico |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Reis, Célia Rosimarie dos Lopes, Fabiana Maria Ruiz Gonçalves, Daniela Dib Freire, Roberta Lemos Garcia, João Luis Navarro, Italmar Teodorico |
dc.subject.por.fl_str_mv |
Toxoplasma gondii Imunofluorescência indireta Teste de aglutinação modificada Caprinos Problemas reprodutivos Toxoplasma gondii Indirect immunofluorescence Modified agglutination test Caprines Reproductive problems |
topic |
Toxoplasma gondii Imunofluorescência indireta Teste de aglutinação modificada Caprinos Problemas reprodutivos Toxoplasma gondii Indirect immunofluorescence Modified agglutination test Caprines Reproductive problems |
description |
The aim of this study was to verify the prevalence of anti-Toxoplasma gondii antibodies in sera from naturally infected milk-producing caprines using indirect immunofluorescence assay (IFA) and modified agglutination test (MAT), determine specificity and sensibility of MAT and to evaluate the agreement of results between the tests. For this study, 282 caprine serum samples were collected from farms in the city of Pitanga, Paraná State, a region with a high pecuary of small ruminants. Prevalence found by IFA was of 44.68% and 23.05% by MAT. There was statistically significant difference (p=0.0086) in relation to the gender of animals, being females more seropositive. Sensitivity and specificity results for MAT were, respectively, 42.2% and 94.9%. By comparing results, a moderate agreement was observed (Kappa = 0.42). The high prevalence for the infection, associated to high titers found by IFA and clinical signs presented in the herds suggest toxoplasmosis as one of the causes of reproductive problems in caprines. Sensitivity and specificity results for MAT obtained in this study allow this test to be recommended as a confirmatory test for the caprine species. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26619 10.11606/issn.1678-4456.bjvras.2007.26619 |
url |
https://www.revistas.usp.br/bjvras/article/view/26619 |
identifier_str_mv |
10.11606/issn.1678-4456.bjvras.2007.26619 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26619/28402 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 Núm. 5 (2007); 358-363 Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 No. 5 (2007); 358-363 Brazilian Journal of Veterinary Research and Animal Science; v. 44 n. 5 (2007); 358-363 Brazilian Journal of Veterinary Research and Animal Science; V. 44 N. 5 (2007); 358-363 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051558349766656 |