Detection of Chlamydophila felis and Feline Herpesvirus Type-1 in non-domestic felids in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/90277 |
Resumo: | Little is known about the occurrence of feline upper respiratory tract disease agents, namely Feline Herpesvirus type 1 (FHV-1) and Chlamydophila felis, and co-infection of these agents with Feline Immunodeficiency virus (FIV) and Feline Leukemia Virus (FeLV) in non-domestic felids in Brazil. Between 2009 and 2010, 72 conjunctival swab and serum samples were collected from eight non-domestic felid species (Leopardus pardalis, Leopardus tigrinus, Panthera leo, Panthera tigris, Puma concolor, Puma yagouaroundi, Oncifelis colocolo, and Panthera onca) maintained in captivity in Brazilian zoos. DNA extracted from conjunctival swabs were used in PCR assays for the detection of Chlamydophila sp, FHV-1, and retrovirus DNA, respectively. Antibodies to FIV and FeLV antigen were detected in non-domestic felid serum samples using a commercial ELISA kit. Antibodies to FIV were found only in five (6.9%) felids. No sampled non-domestic felid was positive for FeLV antigen detection. One (1.3%) out of 72 non-domestic felid conjunctival swab samples was positive for Chlamydophilasp. and Feline Herpesvirus-1 in PCR. This felid was an ocelot and was negative for FIV and FeLV. The results of this survey showed the occurrence of co-infection with C. felis and FHV-1 in an ocelot (Leopardus pardalis) in Brazil. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Detection of Chlamydophila felis and Feline Herpesvirus Type-1 in non-domestic felids in BrazilDetecção de Chlamydophila felis e Herpesvirus felino tipo 1 em felídeo não doméstico no BrasilChlamydiaJaguatiricaPCRSwabChlamydiaOcelotPCRSwabLittle is known about the occurrence of feline upper respiratory tract disease agents, namely Feline Herpesvirus type 1 (FHV-1) and Chlamydophila felis, and co-infection of these agents with Feline Immunodeficiency virus (FIV) and Feline Leukemia Virus (FeLV) in non-domestic felids in Brazil. Between 2009 and 2010, 72 conjunctival swab and serum samples were collected from eight non-domestic felid species (Leopardus pardalis, Leopardus tigrinus, Panthera leo, Panthera tigris, Puma concolor, Puma yagouaroundi, Oncifelis colocolo, and Panthera onca) maintained in captivity in Brazilian zoos. DNA extracted from conjunctival swabs were used in PCR assays for the detection of Chlamydophila sp, FHV-1, and retrovirus DNA, respectively. Antibodies to FIV and FeLV antigen were detected in non-domestic felid serum samples using a commercial ELISA kit. Antibodies to FIV were found only in five (6.9%) felids. No sampled non-domestic felid was positive for FeLV antigen detection. One (1.3%) out of 72 non-domestic felid conjunctival swab samples was positive for Chlamydophilasp. and Feline Herpesvirus-1 in PCR. This felid was an ocelot and was negative for FIV and FeLV. The results of this survey showed the occurrence of co-infection with C. felis and FHV-1 in an ocelot (Leopardus pardalis) in Brazil. Poucos trabalhos descrevem a ocorrência dos agentes do complexo respiratório felino, Herpesvírus Felino tipo 1 (FHV-1) e Chlamydophila felis, e a coinfecção com o vírus da imunodeficiência felina (FIV) e leucemia viral felina (FeLV) em felinos não domésticos no Brasil. Entre 2009 e 2010, 72 amostras de swab de conjuntiva e de soro foram coletados de oito espécies de felinos não domésticos (Leopardus pardalis, Leopardus tigrinus, Panthera leo, Panthera tigris, Puma concolor, Puma yagouaroundi, Oncifelis colocolo, and Panthera onca) mantidos em cativeiro em zoológicos brasileiros. O DNA foi extraído das amostras de swab de conjuntiva para detecção de Chlamydophila sp e FHV-1 pela PCR. Anticorpos para FIV e antígeno para FeLV foram determinados pelo kit comercial de ELISA. Anticorpos para FIV foram detectados em cinco felídeos (6,9%). Nenhuma amostra foi positiva para a presença de antígeno de FeLV. Um (1,3%) dos 72 felinos não domésticos apresentou fragmentos de DNA de Chlamydophila sp e FHV-1 pela PCR. Este felino era uma jaguatirica que não apresentou anticorpos para FIV e nem antígeno para FelV. Estes resultados demonstram a ocorrência de coinfecção de C. felis e FHV-1 em uma jaguatirica (Leopardus pardalis) no Brasil.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2016-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/9027710.11606/issn.1678-4456.v53i2p169-176Brazilian Journal of Veterinary Research and Animal Science; Vol. 53 Núm. 2 (2016); 169-176Brazilian Journal of Veterinary Research and Animal Science; Vol. 53 No. 2 (2016); 169-176Brazilian Journal of Veterinary Research and Animal Science; v. 53 n. 2 (2016); 169-176Brazilian Journal of Veterinary Research and Animal Science; V. 53 N. 2 (2016); 169-1761678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/90277/114745Copyright (c) 2016 Brazilian Journal of Veterinary Research and Animal Scienceinfo:eu-repo/semantics/openAccessSeki, Meire ChristinaAndré, Marcos RogérioCarrasco, Adriano de Oliveira TorresMachado, Rosangela ZacariasPinto, Aramis Augusto2020-06-23T04:04:49Zoai:revistas.usp.br:article/90277Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:54.603421Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Detection of Chlamydophila felis and Feline Herpesvirus Type-1 in non-domestic felids in Brazil Detecção de Chlamydophila felis e Herpesvirus felino tipo 1 em felídeo não doméstico no Brasil |
title |
Detection of Chlamydophila felis and Feline Herpesvirus Type-1 in non-domestic felids in Brazil |
spellingShingle |
Detection of Chlamydophila felis and Feline Herpesvirus Type-1 in non-domestic felids in Brazil Seki, Meire Christina Chlamydia Jaguatirica PCR Swab Chlamydia Ocelot PCR Swab |
title_short |
Detection of Chlamydophila felis and Feline Herpesvirus Type-1 in non-domestic felids in Brazil |
title_full |
Detection of Chlamydophila felis and Feline Herpesvirus Type-1 in non-domestic felids in Brazil |
title_fullStr |
Detection of Chlamydophila felis and Feline Herpesvirus Type-1 in non-domestic felids in Brazil |
title_full_unstemmed |
Detection of Chlamydophila felis and Feline Herpesvirus Type-1 in non-domestic felids in Brazil |
title_sort |
Detection of Chlamydophila felis and Feline Herpesvirus Type-1 in non-domestic felids in Brazil |
author |
Seki, Meire Christina |
author_facet |
Seki, Meire Christina André, Marcos Rogério Carrasco, Adriano de Oliveira Torres Machado, Rosangela Zacarias Pinto, Aramis Augusto |
author_role |
author |
author2 |
André, Marcos Rogério Carrasco, Adriano de Oliveira Torres Machado, Rosangela Zacarias Pinto, Aramis Augusto |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Seki, Meire Christina André, Marcos Rogério Carrasco, Adriano de Oliveira Torres Machado, Rosangela Zacarias Pinto, Aramis Augusto |
dc.subject.por.fl_str_mv |
Chlamydia Jaguatirica PCR Swab Chlamydia Ocelot PCR Swab |
topic |
Chlamydia Jaguatirica PCR Swab Chlamydia Ocelot PCR Swab |
description |
Little is known about the occurrence of feline upper respiratory tract disease agents, namely Feline Herpesvirus type 1 (FHV-1) and Chlamydophila felis, and co-infection of these agents with Feline Immunodeficiency virus (FIV) and Feline Leukemia Virus (FeLV) in non-domestic felids in Brazil. Between 2009 and 2010, 72 conjunctival swab and serum samples were collected from eight non-domestic felid species (Leopardus pardalis, Leopardus tigrinus, Panthera leo, Panthera tigris, Puma concolor, Puma yagouaroundi, Oncifelis colocolo, and Panthera onca) maintained in captivity in Brazilian zoos. DNA extracted from conjunctival swabs were used in PCR assays for the detection of Chlamydophila sp, FHV-1, and retrovirus DNA, respectively. Antibodies to FIV and FeLV antigen were detected in non-domestic felid serum samples using a commercial ELISA kit. Antibodies to FIV were found only in five (6.9%) felids. No sampled non-domestic felid was positive for FeLV antigen detection. One (1.3%) out of 72 non-domestic felid conjunctival swab samples was positive for Chlamydophilasp. and Feline Herpesvirus-1 in PCR. This felid was an ocelot and was negative for FIV and FeLV. The results of this survey showed the occurrence of co-infection with C. felis and FHV-1 in an ocelot (Leopardus pardalis) in Brazil. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/90277 10.11606/issn.1678-4456.v53i2p169-176 |
url |
https://www.revistas.usp.br/bjvras/article/view/90277 |
identifier_str_mv |
10.11606/issn.1678-4456.v53i2p169-176 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/90277/114745 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2016 Brazilian Journal of Veterinary Research and Animal Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2016 Brazilian Journal of Veterinary Research and Animal Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 53 Núm. 2 (2016); 169-176 Brazilian Journal of Veterinary Research and Animal Science; Vol. 53 No. 2 (2016); 169-176 Brazilian Journal of Veterinary Research and Animal Science; v. 53 n. 2 (2016); 169-176 Brazilian Journal of Veterinary Research and Animal Science; V. 53 N. 2 (2016); 169-176 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051566663925760 |