Different Follicle-Stimulating Hormone (FSH) sources influence caprine preantral follicle viability and development in vitro

• Autor(a) principal: Magalhães, Deborah de Melo
• Data de Publicação: 2009
• Outros Autores: Araujo, Valdevane Rocha, Verde, Isabel Bezerra Lima, Matos, Maria Helena Tavares de, Silva, Renata Carvalho, Lucci, Carolina Madeira, Báo, Sônia Nair, Campello, Cláudio Cabral, Figueiredo, José Ricardo de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
• Texto Completo: https://www.revistas.usp.br/bjvras/article/view/26787
• topic: Caprine; FSH; Growth; Preantral follicles; Caprinos; FSH; Crescimento folicular; Folículos pré-antrais

The aim of this study was to evaluate the effects of pituitary (pFSH) or recombinant (rFSH) FSH on the survival and growth of caprine preantral follicles. Caprine ovarian tissues were in vitro cultured for one or seven days in Minimum Essential Medium (MEM) alone or containing 10, 50, 100 and 1000 ng/ml of pFSH or rFSH. Control tissues (non-cultured) and those cultured were processed for histological and ultrastructural studies. In addition, follicular and oocyte diameter were analysed. After seven days of culture, only 50 ng/ml of rFSH maintained the percentage of normal follicles similar to control. Moreover, 10 ng/ml of pFSH and all the concentrations of rFSH promoted primordial follicles activation. In addition, the presence of 50 ng/ml of rFSH promoted the highest follicular diameter at day seven of culture. In conclusion, 50 ng/ml of rFSH maintained the ultrastructural integrity of caprine preantral follicles, promoted primordial follicles activation and further growth of cultured follicles.