Zygotes and two cells mouse embryos in co-culture of mouse and bovine epithelial cells in CZB glucose free, TCM199 and Ménézo B2 mediuns
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/5727 |
Resumo: | Mouse embryos were cultured and co-cultured in mouse (MOEC) and bovine (BOEC) oviduct epithelial cells suspension. Embryos were co-cultured from one cell and two-cell stage to blastocyst stage. Cell culture (MOEC and BOEC) was made at a 100 µl drop under oil. Embryos were recovered 24-26 hours in the case of zygotes and 46-48 hours in the case of 2 cell embryos after hCG injection. Total embryos were randomly split into 3 groups: control (cultured), MOEC and BOEC (co-cultured) with CZB, TCM199 and B2 mediums. In CZB group, 44 zygotes (38.26%) from the control achieved blastocyst stage (expanded or hatched), being statistically different (p<=0.05) from MOEC (1.89%) and BOEC (6.72%). Co-culture with zygotes in TCM199 and B2 didnt pass 2 cell block. Two cell embryos cultured in CZB achieved blastocyst stage at a rate of 43.80% for the control, 38.79% for MOEC and 29.60% for BOEC group. Statistical difference (p<=0.05) appears from the control with BOEC groups. In TCM 199 medium, total of blastocyst were 48.15% for the control, 39.08% for MOEC and 48.49% for the BOEC groups, having no statistical difference among groups. This study allowed concluding that zygote block in TCM199 and B2 medium may be related to the presence of glucose. And that mouse embryos development is not species-specific. |
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oai:revistas.usp.br:article/5727 |
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USP-49 |
network_name_str |
Brazilian Journal of Veterinary Research and Animal Science |
repository_id_str |
https://www.revistas.usp.br/bjvras/index |
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Zygotes and two cells mouse embryos in co-culture of mouse and bovine epithelial cells in CZB glucose free, TCM199 and Ménézo B2 mediunsCo-cultivo de zigotos e embriões de duas células de camundongos (Mus musculus domesticus) em suspensão de células epiteliais de ovidutos de camundongos e bovinos nos meios CZB sem glicose, TCM199 e Ménézo B2Cultivos celularesCélulas epiteliaisEmbriõesCamundongosCells culturedEpithelial cellsEmbryosMiceMouse embryos were cultured and co-cultured in mouse (MOEC) and bovine (BOEC) oviduct epithelial cells suspension. Embryos were co-cultured from one cell and two-cell stage to blastocyst stage. Cell culture (MOEC and BOEC) was made at a 100 µl drop under oil. Embryos were recovered 24-26 hours in the case of zygotes and 46-48 hours in the case of 2 cell embryos after hCG injection. Total embryos were randomly split into 3 groups: control (cultured), MOEC and BOEC (co-cultured) with CZB, TCM199 and B2 mediums. In CZB group, 44 zygotes (38.26%) from the control achieved blastocyst stage (expanded or hatched), being statistically different (p<=0.05) from MOEC (1.89%) and BOEC (6.72%). Co-culture with zygotes in TCM199 and B2 didnt pass 2 cell block. Two cell embryos cultured in CZB achieved blastocyst stage at a rate of 43.80% for the control, 38.79% for MOEC and 29.60% for BOEC group. Statistical difference (p<=0.05) appears from the control with BOEC groups. In TCM 199 medium, total of blastocyst were 48.15% for the control, 39.08% for MOEC and 48.49% for the BOEC groups, having no statistical difference among groups. This study allowed concluding that zygote block in TCM199 and B2 medium may be related to the presence of glucose. And that mouse embryos development is not species-specific.Zigotos e embriões de 2 células de camundongos F1 (C57/DBA) foram cultivados (controle) e co-cultivados em suspensões de células epiteliais de ovidutos de camundongos (MOEC) e de bovinos (BOEC) até o estádio de blastocistos nos meios CZB, TCM199 e Ménézo B2. Os zigotos que atingiram os estádios de blastocistos expandido e eclodido no CZB foram 44 (38,26%) para o controle, 2 (1,89%) para as MOEC e 8 (6,72%) para as BOEC, sendo que o controle apresentou-se estatisticamente diferente (p<=0,05) em relação às MOEC e às BOEC. Os zigotos cultivados e co-cultivados nos meios TCM199 e B2 não clivaram além de 2 células. Os embriões de 2 células que desenvolveram até blastocistos no CZB foram 46 (43,80%) para o controle, 45 (38,79%) para as MOEC e 37 (29,60%) para as BOEC, sendo que o controle apresentou-se estatisticamente diferente (p<=0,05) em relação às BOEC. No TCM199, o total de blastocistos foi de 52 (48,15%) para o controle, 68 (39,08%) para as MOEC e 64 (48,49%) para as BOEC, não havendo diferença estatística (p>;0,05) entre os três grupos. No B2, o total de blastocistos foi de 28 (24,78%) para o controle, 34 (28,10%) para as MOEC e 25 (23,81%) para as BOEC, não havendo diferença estatística (p>;0,05) entre os três grupos. Concluiu-se que o bloqueio dos zigotos nos meios TCM199 e B2 pode estar relacionado à presença da glicose e que o oviduto não é espécie-específico quanto a sua capacidade em promover o desenvolvimento de embriões de camundongos.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1999-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/572710.1590/S1413-95961999000600006Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 Núm. 6 (1999); 308-315Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 No. 6 (1999); 308-315Brazilian Journal of Veterinary Research and Animal Science; v. 36 n. 6 (1999); 308-315Brazilian Journal of Veterinary Research and Animal Science; V. 36 N. 6 (1999); 308-3151678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/5727/64810Tavares, Liliam Mara TrerrisanAssumpção, Mayra Elena Ortiz D'AvilaFerreira, FernandoLima, Alecsandra SobreiraMello, Marco Roberto BourgVisintin, José Antonioinfo:eu-repo/semantics/openAccess2020-06-23T04:44:38Zoai:revistas.usp.br:article/5727Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:19.367658Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Zygotes and two cells mouse embryos in co-culture of mouse and bovine epithelial cells in CZB glucose free, TCM199 and Ménézo B2 mediuns Co-cultivo de zigotos e embriões de duas células de camundongos (Mus musculus domesticus) em suspensão de células epiteliais de ovidutos de camundongos e bovinos nos meios CZB sem glicose, TCM199 e Ménézo B2 |
title |
Zygotes and two cells mouse embryos in co-culture of mouse and bovine epithelial cells in CZB glucose free, TCM199 and Ménézo B2 mediuns |
spellingShingle |
Zygotes and two cells mouse embryos in co-culture of mouse and bovine epithelial cells in CZB glucose free, TCM199 and Ménézo B2 mediuns Tavares, Liliam Mara Trerrisan Cultivos celulares Células epiteliais Embriões Camundongos Cells cultured Epithelial cells Embryos Mice |
title_short |
Zygotes and two cells mouse embryos in co-culture of mouse and bovine epithelial cells in CZB glucose free, TCM199 and Ménézo B2 mediuns |
title_full |
Zygotes and two cells mouse embryos in co-culture of mouse and bovine epithelial cells in CZB glucose free, TCM199 and Ménézo B2 mediuns |
title_fullStr |
Zygotes and two cells mouse embryos in co-culture of mouse and bovine epithelial cells in CZB glucose free, TCM199 and Ménézo B2 mediuns |
title_full_unstemmed |
Zygotes and two cells mouse embryos in co-culture of mouse and bovine epithelial cells in CZB glucose free, TCM199 and Ménézo B2 mediuns |
title_sort |
Zygotes and two cells mouse embryos in co-culture of mouse and bovine epithelial cells in CZB glucose free, TCM199 and Ménézo B2 mediuns |
author |
Tavares, Liliam Mara Trerrisan |
author_facet |
Tavares, Liliam Mara Trerrisan Assumpção, Mayra Elena Ortiz D'Avila Ferreira, Fernando Lima, Alecsandra Sobreira Mello, Marco Roberto Bourg Visintin, José Antonio |
author_role |
author |
author2 |
Assumpção, Mayra Elena Ortiz D'Avila Ferreira, Fernando Lima, Alecsandra Sobreira Mello, Marco Roberto Bourg Visintin, José Antonio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Tavares, Liliam Mara Trerrisan Assumpção, Mayra Elena Ortiz D'Avila Ferreira, Fernando Lima, Alecsandra Sobreira Mello, Marco Roberto Bourg Visintin, José Antonio |
dc.subject.por.fl_str_mv |
Cultivos celulares Células epiteliais Embriões Camundongos Cells cultured Epithelial cells Embryos Mice |
topic |
Cultivos celulares Células epiteliais Embriões Camundongos Cells cultured Epithelial cells Embryos Mice |
description |
Mouse embryos were cultured and co-cultured in mouse (MOEC) and bovine (BOEC) oviduct epithelial cells suspension. Embryos were co-cultured from one cell and two-cell stage to blastocyst stage. Cell culture (MOEC and BOEC) was made at a 100 µl drop under oil. Embryos were recovered 24-26 hours in the case of zygotes and 46-48 hours in the case of 2 cell embryos after hCG injection. Total embryos were randomly split into 3 groups: control (cultured), MOEC and BOEC (co-cultured) with CZB, TCM199 and B2 mediums. In CZB group, 44 zygotes (38.26%) from the control achieved blastocyst stage (expanded or hatched), being statistically different (p<=0.05) from MOEC (1.89%) and BOEC (6.72%). Co-culture with zygotes in TCM199 and B2 didnt pass 2 cell block. Two cell embryos cultured in CZB achieved blastocyst stage at a rate of 43.80% for the control, 38.79% for MOEC and 29.60% for BOEC group. Statistical difference (p<=0.05) appears from the control with BOEC groups. In TCM 199 medium, total of blastocyst were 48.15% for the control, 39.08% for MOEC and 48.49% for the BOEC groups, having no statistical difference among groups. This study allowed concluding that zygote block in TCM199 and B2 medium may be related to the presence of glucose. And that mouse embryos development is not species-specific. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5727 10.1590/S1413-95961999000600006 |
url |
https://www.revistas.usp.br/bjvras/article/view/5727 |
identifier_str_mv |
10.1590/S1413-95961999000600006 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5727/64810 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 Núm. 6 (1999); 308-315 Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 No. 6 (1999); 308-315 Brazilian Journal of Veterinary Research and Animal Science; v. 36 n. 6 (1999); 308-315 Brazilian Journal of Veterinary Research and Animal Science; V. 36 N. 6 (1999); 308-315 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051554629419008 |