The carcinogenic potential of cadmium in the palatal and gingival epithelium of rats: a morphologic and morphometric analysis
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/6275 |
Resumo: | Cadmium (Cd) is a heavy metal that exerts a variety of toxic effects, chronic and acute, in exposed organisms. The aim of this study was to investigate the carcinogenic potential of Cd in the palatal and gingival epithelium of Wistar rats. Two groups of animals were studied: group 1 consisted of 5 rats exposed to cadmium chloride (CdCl2) in drinking water (300 mg/L) for 6 months; group 2 also consisted of 5 rats placed in the same conditions as those of group 1, but kept free of Cd for 6 additional months. Two other groups (C1 and C2) with the same number of animals, but not exposed to CdCl2, were used as a control for groups 1 and 2, respectively. All animals were weighed before and after the experimental period. After animals being killed, tissues of interest were fixed in solution of 10% formalin, processed by standard histologic techniques, stained with HE, and analyzed under light microscopy using karyometric and stereologic parameters. Loss of body weight, atrophy of the gingiva and soft-palate epithelium were the principal findings of this study, and verified only in the group 1 (p < 0.05). In conclusion, at the concentration tested, Cd presented no carcinogenic effect on the oral tissues within the experimental time period. |
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Brazilian Journal of Veterinary Research and Animal Science |
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The carcinogenic potential of cadmium in the palatal and gingival epithelium of rats: a morphologic and morphometric analysisAvaliação do potencial carcinogênico do cadmio no epitélio de revestimento do palato e gengiva de ratos: estudo morfológico e morfométricoCloreto de cádmioCancer oralCarcinogêneseRatoCadmium chlorideOoral cancerCarcinogenesisRatCadmium (Cd) is a heavy metal that exerts a variety of toxic effects, chronic and acute, in exposed organisms. The aim of this study was to investigate the carcinogenic potential of Cd in the palatal and gingival epithelium of Wistar rats. Two groups of animals were studied: group 1 consisted of 5 rats exposed to cadmium chloride (CdCl2) in drinking water (300 mg/L) for 6 months; group 2 also consisted of 5 rats placed in the same conditions as those of group 1, but kept free of Cd for 6 additional months. Two other groups (C1 and C2) with the same number of animals, but not exposed to CdCl2, were used as a control for groups 1 and 2, respectively. All animals were weighed before and after the experimental period. After animals being killed, tissues of interest were fixed in solution of 10% formalin, processed by standard histologic techniques, stained with HE, and analyzed under light microscopy using karyometric and stereologic parameters. Loss of body weight, atrophy of the gingiva and soft-palate epithelium were the principal findings of this study, and verified only in the group 1 (p < 0.05). In conclusion, at the concentration tested, Cd presented no carcinogenic effect on the oral tissues within the experimental time period.Cádmio (Cd) é um metal pesado que exerce uma variedade de efeitos tóxicos, crônicos e agudos, em organismos expostos. O objetivo deste estudo foi investigar o potencial carcinogênico do Cd no epitélio do palato e da gengiva de ratos Wistar. Dois grupos de animais foram estudados: grupo 1 que consistiu de 5 ratos expostos a cloreto de cádmio (CdCl2) em água de bebedouro (300 mg/L) por um período de 6 meses; grupo 2 que também consistiu de 5 ratos submetidos às mesmas condições dos animais do grupo 1, mas permaneceu livre de Cd por um período adicional de 6 meses. Dois outros grupos (C1 and C2) com o mesmo número de animais, entretanto não expostos ao CdCl2, foram usados como controle para o grupo 1 e 2, respectivamente. Todos os animais foram pesados antes e após o período experimental. Após terem sido sacrificados, os tecidos de interesse para o estudo foram fixados em formalina a 10%, processados por meio de técnica histopatológica padrão, corados em HE, e analisados sob microscopia de luz, utilizando parâmetros cariométricos e estereológicos. Perda de peso, atrofia do epitélio da gengiva e do palato mole foram os principais achados deste estudo, e verificados apenas no grupo 1 (p < 0.05). Em conclusão, Cd não produziu efeito carcinogênico nos tecidos orais, nas condições experimentais empregadas neste estudo.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2004-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/627510.1590/S1413-95962004000300006Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 Núm. 3 (2004); 183-188 Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 No. 3 (2004); 183-188 Brazilian Journal of Veterinary Research and Animal Science; v. 41 n. 3 (2004); 183-188 Brazilian Journal of Veterinary Research and Animal Science; V. 41 N. 3 (2004); 183-188 1678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/6275/7806Motta, Ana Carolina FragosoMigliari, Dante AntonioGioso, Marco AntonioKomesu, Marilena ChinaliSala, Miguel AngelLopes, Ruberval Armandoinfo:eu-repo/semantics/openAccess2020-06-23T04:29:37Zoai:revistas.usp.br:article/6275Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:35.003587Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
The carcinogenic potential of cadmium in the palatal and gingival epithelium of rats: a morphologic and morphometric analysis Avaliação do potencial carcinogênico do cadmio no epitélio de revestimento do palato e gengiva de ratos: estudo morfológico e morfométrico |
title |
The carcinogenic potential of cadmium in the palatal and gingival epithelium of rats: a morphologic and morphometric analysis |
spellingShingle |
The carcinogenic potential of cadmium in the palatal and gingival epithelium of rats: a morphologic and morphometric analysis Motta, Ana Carolina Fragoso Cloreto de cádmio Cancer oral Carcinogênese Rato Cadmium chloride Ooral cancer Carcinogenesis Rat |
title_short |
The carcinogenic potential of cadmium in the palatal and gingival epithelium of rats: a morphologic and morphometric analysis |
title_full |
The carcinogenic potential of cadmium in the palatal and gingival epithelium of rats: a morphologic and morphometric analysis |
title_fullStr |
The carcinogenic potential of cadmium in the palatal and gingival epithelium of rats: a morphologic and morphometric analysis |
title_full_unstemmed |
The carcinogenic potential of cadmium in the palatal and gingival epithelium of rats: a morphologic and morphometric analysis |
title_sort |
The carcinogenic potential of cadmium in the palatal and gingival epithelium of rats: a morphologic and morphometric analysis |
author |
Motta, Ana Carolina Fragoso |
author_facet |
Motta, Ana Carolina Fragoso Migliari, Dante Antonio Gioso, Marco Antonio Komesu, Marilena Chinali Sala, Miguel Angel Lopes, Ruberval Armando |
author_role |
author |
author2 |
Migliari, Dante Antonio Gioso, Marco Antonio Komesu, Marilena Chinali Sala, Miguel Angel Lopes, Ruberval Armando |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Motta, Ana Carolina Fragoso Migliari, Dante Antonio Gioso, Marco Antonio Komesu, Marilena Chinali Sala, Miguel Angel Lopes, Ruberval Armando |
dc.subject.por.fl_str_mv |
Cloreto de cádmio Cancer oral Carcinogênese Rato Cadmium chloride Ooral cancer Carcinogenesis Rat |
topic |
Cloreto de cádmio Cancer oral Carcinogênese Rato Cadmium chloride Ooral cancer Carcinogenesis Rat |
description |
Cadmium (Cd) is a heavy metal that exerts a variety of toxic effects, chronic and acute, in exposed organisms. The aim of this study was to investigate the carcinogenic potential of Cd in the palatal and gingival epithelium of Wistar rats. Two groups of animals were studied: group 1 consisted of 5 rats exposed to cadmium chloride (CdCl2) in drinking water (300 mg/L) for 6 months; group 2 also consisted of 5 rats placed in the same conditions as those of group 1, but kept free of Cd for 6 additional months. Two other groups (C1 and C2) with the same number of animals, but not exposed to CdCl2, were used as a control for groups 1 and 2, respectively. All animals were weighed before and after the experimental period. After animals being killed, tissues of interest were fixed in solution of 10% formalin, processed by standard histologic techniques, stained with HE, and analyzed under light microscopy using karyometric and stereologic parameters. Loss of body weight, atrophy of the gingiva and soft-palate epithelium were the principal findings of this study, and verified only in the group 1 (p < 0.05). In conclusion, at the concentration tested, Cd presented no carcinogenic effect on the oral tissues within the experimental time period. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/6275 10.1590/S1413-95962004000300006 |
url |
https://www.revistas.usp.br/bjvras/article/view/6275 |
identifier_str_mv |
10.1590/S1413-95962004000300006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/6275/7806 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 Núm. 3 (2004); 183-188 Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 No. 3 (2004); 183-188 Brazilian Journal of Veterinary Research and Animal Science; v. 41 n. 3 (2004); 183-188 Brazilian Journal of Veterinary Research and Animal Science; V. 41 N. 3 (2004); 183-188 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
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1797051556337549312 |