Comparison of the broth microdilution technique and ETEST to ketoconazole front Malassezia pachydermatis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/26770 |
Resumo: | Malassezia pachydermatis is recognized as a normal inhabitant and an opportunistic pathogen of the external ear canal and skin of dogs and cats. In special clinical conditions, and mainly in the cases of therapeutic failure related to external otitis and dermatitis complicated by this yeast, is recommended testing susceptibility to antifungal drugs. Different approaches of evaluating the susceptibility of yeasts faced to antifungals in laboratory exist, some of them are commercial approaches and others previously standardized by the CLSI (NCCLS, 2002). The purpose of this study was to evaluate the susceptibility of 17 samples of M. pachydermatis from canine external otitis using two different in vitro antifungal susceptibility methods: the Etest® and the broth Microdilution Method (MD) with ketoconazole. The mean MIC observed between the 17 samples were 0.103mg/mL to ETEST and 0.0012mg/mL to MD ranging from 0.004 to 0.75mg/mL in ETEST and 0.0019 and 0.03mg/mL in MD using the same samples. By ETEST, two (11.8%) samples were resistant, eight (47.1%) susceptible and seven (41.1%) showed intermediate susceptibility. Through the MD it was observed four (23.5%) resistant samples, seven (41.2%) susceptible and six (35.3%) samples with intermediate susceptibility. Despite of the percentages being equivalent in each rank of susceptibility through the two techniques, the results do not correspond to the same sample. These results showed that there is an urgent need to standardize those values considered as parameters for growth inhibition of this yeast. Then a simple and efficient method could be used routinely in the laboratory practice. |
id |
USP-49_fdd4ab104eae70e696ea9a8b2c617862 |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/26770 |
network_acronym_str |
USP-49 |
network_name_str |
Brazilian Journal of Veterinary Research and Animal Science |
repository_id_str |
https://www.revistas.usp.br/bjvras/index |
spelling |
Comparison of the broth microdilution technique and ETEST to ketoconazole front Malassezia pachydermatisComparação da técnica de microdiluição em caldo e ETEST para o cetoconazol frente à Malassezia pachydermatisMalassezia pachydermatisCLSIETESTMicrodiluição em caldoMalassezia pachydermatisCLSIETESTBroth microdilutionMalassezia pachydermatis is recognized as a normal inhabitant and an opportunistic pathogen of the external ear canal and skin of dogs and cats. In special clinical conditions, and mainly in the cases of therapeutic failure related to external otitis and dermatitis complicated by this yeast, is recommended testing susceptibility to antifungal drugs. Different approaches of evaluating the susceptibility of yeasts faced to antifungals in laboratory exist, some of them are commercial approaches and others previously standardized by the CLSI (NCCLS, 2002). The purpose of this study was to evaluate the susceptibility of 17 samples of M. pachydermatis from canine external otitis using two different in vitro antifungal susceptibility methods: the Etest® and the broth Microdilution Method (MD) with ketoconazole. The mean MIC observed between the 17 samples were 0.103mg/mL to ETEST and 0.0012mg/mL to MD ranging from 0.004 to 0.75mg/mL in ETEST and 0.0019 and 0.03mg/mL in MD using the same samples. By ETEST, two (11.8%) samples were resistant, eight (47.1%) susceptible and seven (41.1%) showed intermediate susceptibility. Through the MD it was observed four (23.5%) resistant samples, seven (41.2%) susceptible and six (35.3%) samples with intermediate susceptibility. Despite of the percentages being equivalent in each rank of susceptibility through the two techniques, the results do not correspond to the same sample. These results showed that there is an urgent need to standardize those values considered as parameters for growth inhibition of this yeast. Then a simple and efficient method could be used routinely in the laboratory practice.Malassezia pachydermatis é reconhecida como um habitante normal e patógeno oportunista do meato acústico externo e da pele de cães e gatos. Em condições clínicas especiais e em casos de falha terapêutica relatada em otite externa e dermatite complicada por esta levedura, é recomendado o teste de suscetibilidade antifúngica. Existem diferentes métodos de avaliação da suscetibilidade da levedura frente a antifúngicos em laboratório, alguns métodos comerciais e outros previamente padronizados pelo CLSI (NCCLS, 2002). O objetivo deste estudo foi o de avaliar a suscetibilidade de 17 amostras de M. pachydermatis proveniente de otite externa canina por meio de duas técnicas in vitro de antifungigramas: o Etest® e a microdiluição em caldo (MC) com o cetoconazol. A media da Concentração Inibitória Mínima (CIM) observada entre as 17 amostras foram 0.103mg/mL para o ETEST e 0.0012mg/mL para a MC variando de 0.004 a 0.75mg/mL no ETEST e entre 0.0019 e 0.03mg/mL na MC usando as mesmas amostras. Pelo ETEST, duas (11.8%) amostras foram resistentes, oito (47.1%) sensíveis e sete (41.1%) mostraram sensibilidade intermediária. Na MC foram observadas quatro (23.5%) amostras resistentes, sete (41.2%) sensíveis e seis (35.3%) amostras com sensibilidade intermediária. Apesar das porcentagens de sensibilidade serem semelhantes pelas duas técnicas, os resultados de CIM não correspondem na uma mesma amostra. Estes resultados mostraram que há uma urgente necessidade de padronização dos valores considerados como parâmetros para inibição do crescimento da levedura. Portanto, um método simples e eficiente deveria ser usado rotina na prática de laboratório.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2009-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2677010.11606/issn.1678-4456.bjvras.2009.26770Brazilian Journal of Veterinary Research and Animal Science; Vol. 46 Núm. 3 (2009); 222-227Brazilian Journal of Veterinary Research and Animal Science; Vol. 46 No. 3 (2009); 222-227Brazilian Journal of Veterinary Research and Animal Science; v. 46 n. 3 (2009); 222-227Brazilian Journal of Veterinary Research and Animal Science; V. 46 N. 3 (2009); 222-2271678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/26770/28553Nascente, Patrícia da SilvaCleff, Marlete BrumMeinerz, Ana Raquel ManoXavier, Melissa OrzechowskiSchuch, Luiz Filipe DaméMeireles, Mário Carlos AraújoMello, João Roberto Braga deinfo:eu-repo/semantics/openAccess2020-06-23T04:11:45Zoai:revistas.usp.br:article/26770Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:02.916137Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Comparison of the broth microdilution technique and ETEST to ketoconazole front Malassezia pachydermatis Comparação da técnica de microdiluição em caldo e ETEST para o cetoconazol frente à Malassezia pachydermatis |
title |
Comparison of the broth microdilution technique and ETEST to ketoconazole front Malassezia pachydermatis |
spellingShingle |
Comparison of the broth microdilution technique and ETEST to ketoconazole front Malassezia pachydermatis Nascente, Patrícia da Silva Malassezia pachydermatis CLSI ETEST Microdiluição em caldo Malassezia pachydermatis CLSI ETEST Broth microdilution |
title_short |
Comparison of the broth microdilution technique and ETEST to ketoconazole front Malassezia pachydermatis |
title_full |
Comparison of the broth microdilution technique and ETEST to ketoconazole front Malassezia pachydermatis |
title_fullStr |
Comparison of the broth microdilution technique and ETEST to ketoconazole front Malassezia pachydermatis |
title_full_unstemmed |
Comparison of the broth microdilution technique and ETEST to ketoconazole front Malassezia pachydermatis |
title_sort |
Comparison of the broth microdilution technique and ETEST to ketoconazole front Malassezia pachydermatis |
author |
Nascente, Patrícia da Silva |
author_facet |
Nascente, Patrícia da Silva Cleff, Marlete Brum Meinerz, Ana Raquel Mano Xavier, Melissa Orzechowski Schuch, Luiz Filipe Damé Meireles, Mário Carlos Araújo Mello, João Roberto Braga de |
author_role |
author |
author2 |
Cleff, Marlete Brum Meinerz, Ana Raquel Mano Xavier, Melissa Orzechowski Schuch, Luiz Filipe Damé Meireles, Mário Carlos Araújo Mello, João Roberto Braga de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Nascente, Patrícia da Silva Cleff, Marlete Brum Meinerz, Ana Raquel Mano Xavier, Melissa Orzechowski Schuch, Luiz Filipe Damé Meireles, Mário Carlos Araújo Mello, João Roberto Braga de |
dc.subject.por.fl_str_mv |
Malassezia pachydermatis CLSI ETEST Microdiluição em caldo Malassezia pachydermatis CLSI ETEST Broth microdilution |
topic |
Malassezia pachydermatis CLSI ETEST Microdiluição em caldo Malassezia pachydermatis CLSI ETEST Broth microdilution |
description |
Malassezia pachydermatis is recognized as a normal inhabitant and an opportunistic pathogen of the external ear canal and skin of dogs and cats. In special clinical conditions, and mainly in the cases of therapeutic failure related to external otitis and dermatitis complicated by this yeast, is recommended testing susceptibility to antifungal drugs. Different approaches of evaluating the susceptibility of yeasts faced to antifungals in laboratory exist, some of them are commercial approaches and others previously standardized by the CLSI (NCCLS, 2002). The purpose of this study was to evaluate the susceptibility of 17 samples of M. pachydermatis from canine external otitis using two different in vitro antifungal susceptibility methods: the Etest® and the broth Microdilution Method (MD) with ketoconazole. The mean MIC observed between the 17 samples were 0.103mg/mL to ETEST and 0.0012mg/mL to MD ranging from 0.004 to 0.75mg/mL in ETEST and 0.0019 and 0.03mg/mL in MD using the same samples. By ETEST, two (11.8%) samples were resistant, eight (47.1%) susceptible and seven (41.1%) showed intermediate susceptibility. Through the MD it was observed four (23.5%) resistant samples, seven (41.2%) susceptible and six (35.3%) samples with intermediate susceptibility. Despite of the percentages being equivalent in each rank of susceptibility through the two techniques, the results do not correspond to the same sample. These results showed that there is an urgent need to standardize those values considered as parameters for growth inhibition of this yeast. Then a simple and efficient method could be used routinely in the laboratory practice. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26770 10.11606/issn.1678-4456.bjvras.2009.26770 |
url |
https://www.revistas.usp.br/bjvras/article/view/26770 |
identifier_str_mv |
10.11606/issn.1678-4456.bjvras.2009.26770 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26770/28553 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 46 Núm. 3 (2009); 222-227 Brazilian Journal of Veterinary Research and Animal Science; Vol. 46 No. 3 (2009); 222-227 Brazilian Journal of Veterinary Research and Animal Science; v. 46 n. 3 (2009); 222-227 Brazilian Journal of Veterinary Research and Animal Science; V. 46 N. 3 (2009); 222-227 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051559345913856 |