Development of a Portable Impedance Spectrometer
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da USP |
Texto Completo: | https://doi.org/10.11606/D.76.2022.tde-19072022-120227 |
Resumo: | Electrical impedance spectroscopy has been used extensively for sensing and biosensing due to the multiple electrical properties that can be interrogated through varying the frequency of the electrical excitation. An impedance spectroscopy system comprises three main units: signal processing, sensing and data analysis. This Masters Dissertation starts with a detailed revision of these units. We elaborate upon the methods to fabricate sensing units, including the choice of nanomaterials and biomolecules in controlled molecular architectures. Using arrays of sensing units, as electronic tongues, generates large amounts of data that require the use of data analysis methods, which we also revise and include examples of information visualization and machine learning techniques. The main focus of the revision, however, is on the signal processing unit, responsible for generating the excitation signal and performing the impedance readout. This focus is due to the high cost of the impedance spectrometers available in the market, which hampers integration of biosensing systems to be used in the field. A detailed description is given of the methods to develop a portable, fully integrated low-cost impedance analyzer that offers wide impedance magnitude, signal amplitude and frequency ranges. The device referred to as Simple-Z includes the integrated circuit AD5933 and peripheral circuits that allow for fine output amplitude regulation, flexible response amplification, sampling rate control, automatic calibration and external communication. We were able to fabricate a few units of Simple-Z with reproducible results, develop a graphical user interface and apply it successfully in sensing and biosensing, including for SARS-CoV-2 detection. The validity of Simple-Z was confirmed by comparing its results in some of the applications with those obtained with benchtop commercial impedance analyzers. Simple-Z can now be deployed in point-of-care diagnosis systems. Furthermore, owing to its low cost it can be fabricated with mass production to also be used in teaching labs for training students in electrical impedance spectroscopy. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis Development of a Portable Impedance Spectrometer Desenvolvimento de um espectrômetro de impedância portátil 2022-02-21Osvaldo Novais de Oliveira JuniorCarlos Frederico de Oliveira GraeffLucas Fugikawa SantosLorenzo Antonio BuscagliaUniversidade de São PauloFísicaUSPBR Biosensing Biossensoriamento Espectroscopia de impedância Impedance spectroscopy Processamento de sinais SARS-CoV-2 SARS-CoV-2 Signal processing Simple-Z Simple-Z Electrical impedance spectroscopy has been used extensively for sensing and biosensing due to the multiple electrical properties that can be interrogated through varying the frequency of the electrical excitation. An impedance spectroscopy system comprises three main units: signal processing, sensing and data analysis. This Masters Dissertation starts with a detailed revision of these units. We elaborate upon the methods to fabricate sensing units, including the choice of nanomaterials and biomolecules in controlled molecular architectures. Using arrays of sensing units, as electronic tongues, generates large amounts of data that require the use of data analysis methods, which we also revise and include examples of information visualization and machine learning techniques. The main focus of the revision, however, is on the signal processing unit, responsible for generating the excitation signal and performing the impedance readout. This focus is due to the high cost of the impedance spectrometers available in the market, which hampers integration of biosensing systems to be used in the field. A detailed description is given of the methods to develop a portable, fully integrated low-cost impedance analyzer that offers wide impedance magnitude, signal amplitude and frequency ranges. The device referred to as Simple-Z includes the integrated circuit AD5933 and peripheral circuits that allow for fine output amplitude regulation, flexible response amplification, sampling rate control, automatic calibration and external communication. We were able to fabricate a few units of Simple-Z with reproducible results, develop a graphical user interface and apply it successfully in sensing and biosensing, including for SARS-CoV-2 detection. The validity of Simple-Z was confirmed by comparing its results in some of the applications with those obtained with benchtop commercial impedance analyzers. Simple-Z can now be deployed in point-of-care diagnosis systems. Furthermore, owing to its low cost it can be fabricated with mass production to also be used in teaching labs for training students in electrical impedance spectroscopy. A espectroscopia de impedância elétrica tem sido usada extensivamente para (bio) sensoriamento graças às múltiplas propriedades elétricas que podem ser interrogadas por meio da variação da frequência da excitação. Um sistema de espectroscopia de impedância compreende três unidades principais: processador de sinais, sensor e analisador de dados. Esta Dissertação de Mestrado começa com uma revisão detalhada dessas unidades. Revisamos os métodos para fabricar sensores, incluindo a escolha de nanomateriais e biomoléculas em arquiteturas moleculares controladas. O uso de matrizes de sensores, como línguas eletrônicas, gera grandes quantidades de dados que exigem o uso de métodos de análise de dados que também revisamos e incluímos exemplos de visualização de informações e técnicas de aprendizado de máquina. O foco principal da revisão, entretanto, está no processador de sinais, responsável por gerar o sinal de excitação e realizar a leitura de impedância. Esse foco se deve ao alto custo dos espectrômetros de impedância disponíveis no mercado, o que dificulta a integração de sistemas de biossensoriamento para serem utilizados em campo. É fornecida uma descrição detalhada dos métodos para desenvolver um analisador de impedância portátil, totalmente integrado e de baixo custo que oferece amplas faixas de magnitude de impedância, de amplitude de sinal e de frequência. O dispositivo, nomeado Simple-Z, inclui o circuito integrado AD5933 e circuitos periféricos para regulagem fina de amplitude de saída, amplificação flexível de resposta, controle da frequência de amostragem, calibração automática e comunicação externa. Fabricamos algumas unidades de Simple-Z com resultados reprodutíveis com uma interface gráfica e aplicamos com sucesso em (bio)sensoriamento, incluindo a detecção de SARS-CoV-2. A validade do Simple-Z foi confirmada comparando seus resultados em algumas das aplicações com aqueles obtidos com analisadores de impedância comerciais de bancada. O Simple-Z agora pode ser implantado em sistemas de diagnóstico para uso em campo. Além disso, graças ao seu baixo custo, pode ser fabricado com produção em massa para ser utilizado também em laboratórios de ensino para treinamento de alunos em espectroscopia de impedância elétrica. https://doi.org/10.11606/D.76.2022.tde-19072022-120227info:eu-repo/semantics/openAccessengreponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USP2023-12-21T19:25:20Zoai:teses.usp.br:tde-19072022-120227Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212023-12-22T12:55:18.577865Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false |
dc.title.en.fl_str_mv |
Development of a Portable Impedance Spectrometer |
dc.title.alternative.pt.fl_str_mv |
Desenvolvimento de um espectrômetro de impedância portátil |
title |
Development of a Portable Impedance Spectrometer |
spellingShingle |
Development of a Portable Impedance Spectrometer Lorenzo Antonio Buscaglia |
title_short |
Development of a Portable Impedance Spectrometer |
title_full |
Development of a Portable Impedance Spectrometer |
title_fullStr |
Development of a Portable Impedance Spectrometer |
title_full_unstemmed |
Development of a Portable Impedance Spectrometer |
title_sort |
Development of a Portable Impedance Spectrometer |
author |
Lorenzo Antonio Buscaglia |
author_facet |
Lorenzo Antonio Buscaglia |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Osvaldo Novais de Oliveira Junior |
dc.contributor.referee1.fl_str_mv |
Carlos Frederico de Oliveira Graeff |
dc.contributor.referee2.fl_str_mv |
Lucas Fugikawa Santos |
dc.contributor.author.fl_str_mv |
Lorenzo Antonio Buscaglia |
contributor_str_mv |
Osvaldo Novais de Oliveira Junior Carlos Frederico de Oliveira Graeff Lucas Fugikawa Santos |
description |
Electrical impedance spectroscopy has been used extensively for sensing and biosensing due to the multiple electrical properties that can be interrogated through varying the frequency of the electrical excitation. An impedance spectroscopy system comprises three main units: signal processing, sensing and data analysis. This Masters Dissertation starts with a detailed revision of these units. We elaborate upon the methods to fabricate sensing units, including the choice of nanomaterials and biomolecules in controlled molecular architectures. Using arrays of sensing units, as electronic tongues, generates large amounts of data that require the use of data analysis methods, which we also revise and include examples of information visualization and machine learning techniques. The main focus of the revision, however, is on the signal processing unit, responsible for generating the excitation signal and performing the impedance readout. This focus is due to the high cost of the impedance spectrometers available in the market, which hampers integration of biosensing systems to be used in the field. A detailed description is given of the methods to develop a portable, fully integrated low-cost impedance analyzer that offers wide impedance magnitude, signal amplitude and frequency ranges. The device referred to as Simple-Z includes the integrated circuit AD5933 and peripheral circuits that allow for fine output amplitude regulation, flexible response amplification, sampling rate control, automatic calibration and external communication. We were able to fabricate a few units of Simple-Z with reproducible results, develop a graphical user interface and apply it successfully in sensing and biosensing, including for SARS-CoV-2 detection. The validity of Simple-Z was confirmed by comparing its results in some of the applications with those obtained with benchtop commercial impedance analyzers. Simple-Z can now be deployed in point-of-care diagnosis systems. Furthermore, owing to its low cost it can be fabricated with mass production to also be used in teaching labs for training students in electrical impedance spectroscopy. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022-02-21 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.11606/D.76.2022.tde-19072022-120227 |
url |
https://doi.org/10.11606/D.76.2022.tde-19072022-120227 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo |
dc.publisher.program.fl_str_mv |
Física |
dc.publisher.initials.fl_str_mv |
USP |
dc.publisher.country.fl_str_mv |
BR |
publisher.none.fl_str_mv |
Universidade de São Paulo |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da USP instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Biblioteca Digital de Teses e Dissertações da USP |
collection |
Biblioteca Digital de Teses e Dissertações da USP |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br |
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1794502865464590336 |