Exploratory studies for cryopreservation of Coffea arabica L. seeds
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of Seed Science |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372017000200150 |
Resumo: | Abstract: Cryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests. Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling. |
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Exploratory studies for cryopreservation of Coffea arabica L. seedssilica gelwater contentslow coolingliquid nitrogencoffeeAbstract: Cryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests. Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling.ABRATES - Associação Brasileira de Tecnologia de Sementes2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372017000200150Journal of Seed Science v.39 n.2 2017reponame:Journal of Seed Scienceinstname:Associação Brasileira de Tecnologia de Sementes (ABRATES)instacron:ABRATES10.1590/2317-1545v39n2171123info:eu-repo/semantics/openAccessFigueiredo,Madeleine Alves deCoelho,Stefania Vilas BoasRosa,Sttela Dellyzete Veiga Franco daVilela,Amanda LimaSilva,Luciano Coutinhoeng2017-08-17T00:00:00Zoai:scielo:S2317-15372017000200150Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=2317-1537&lng=en&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||abrates@abrates.org.br2317-15452317-1537opendoar:2017-08-17T00:00Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES)false |
dc.title.none.fl_str_mv |
Exploratory studies for cryopreservation of Coffea arabica L. seeds |
title |
Exploratory studies for cryopreservation of Coffea arabica L. seeds |
spellingShingle |
Exploratory studies for cryopreservation of Coffea arabica L. seeds Figueiredo,Madeleine Alves de silica gel water content slow cooling liquid nitrogen coffee |
title_short |
Exploratory studies for cryopreservation of Coffea arabica L. seeds |
title_full |
Exploratory studies for cryopreservation of Coffea arabica L. seeds |
title_fullStr |
Exploratory studies for cryopreservation of Coffea arabica L. seeds |
title_full_unstemmed |
Exploratory studies for cryopreservation of Coffea arabica L. seeds |
title_sort |
Exploratory studies for cryopreservation of Coffea arabica L. seeds |
author |
Figueiredo,Madeleine Alves de |
author_facet |
Figueiredo,Madeleine Alves de Coelho,Stefania Vilas Boas Rosa,Sttela Dellyzete Veiga Franco da Vilela,Amanda Lima Silva,Luciano Coutinho |
author_role |
author |
author2 |
Coelho,Stefania Vilas Boas Rosa,Sttela Dellyzete Veiga Franco da Vilela,Amanda Lima Silva,Luciano Coutinho |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Figueiredo,Madeleine Alves de Coelho,Stefania Vilas Boas Rosa,Sttela Dellyzete Veiga Franco da Vilela,Amanda Lima Silva,Luciano Coutinho |
dc.subject.por.fl_str_mv |
silica gel water content slow cooling liquid nitrogen coffee |
topic |
silica gel water content slow cooling liquid nitrogen coffee |
description |
Abstract: Cryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests. Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372017000200150 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372017000200150 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/2317-1545v39n2171123 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
ABRATES - Associação Brasileira de Tecnologia de Sementes |
publisher.none.fl_str_mv |
ABRATES - Associação Brasileira de Tecnologia de Sementes |
dc.source.none.fl_str_mv |
Journal of Seed Science v.39 n.2 2017 reponame:Journal of Seed Science instname:Associação Brasileira de Tecnologia de Sementes (ABRATES) instacron:ABRATES |
instname_str |
Associação Brasileira de Tecnologia de Sementes (ABRATES) |
instacron_str |
ABRATES |
institution |
ABRATES |
reponame_str |
Journal of Seed Science |
collection |
Journal of Seed Science |
repository.name.fl_str_mv |
Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES) |
repository.mail.fl_str_mv |
||abrates@abrates.org.br |
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1754212982462611456 |