Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | São Paulo medical journal (Online) |
Texto Completo: | https://periodicosapm.emnuvens.com.br/spmj/article/view/2083 |
Resumo: | In the present study, a combined method (CM) for attaining simultaneous identification of leukemic cell morphology, karyotype and immunophenotype has been evaluated in 21 patients with acute leukemia and 1 with CML in blast crisis were studied for morphology, citochemistry, immunophenotype and karyotype. Karyotype was performed in a bone marrow sample by using conventional techniques. In each case, direct method (DM) and/or three cultures were tried. The CM consisted in separating a small part of the material resulting from any of the cultures or DM, preparing slides through cytospin and immunophenotyping through APAAP method using the same monoclonal antibodies (MoAb) as for diagnosis. In 14 cases, the metaphases proved positive to the MoAb: in 4, the cells with abnormality had their origin defined; in other 4 the karyotype was normal preventing any identification; 6 cases had minimal abnormalities not visible through CM; and in two cases abnormal karyotypes were detected only in the cultures with GM-CSF. This study showed that CM is feasible in cases where evident numerical or structural chromosomal abnormalties are present. |
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Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemiasLeukemiachromosomesimunophenotypeIn the present study, a combined method (CM) for attaining simultaneous identification of leukemic cell morphology, karyotype and immunophenotype has been evaluated in 21 patients with acute leukemia and 1 with CML in blast crisis were studied for morphology, citochemistry, immunophenotype and karyotype. Karyotype was performed in a bone marrow sample by using conventional techniques. In each case, direct method (DM) and/or three cultures were tried. The CM consisted in separating a small part of the material resulting from any of the cultures or DM, preparing slides through cytospin and immunophenotyping through APAAP method using the same monoclonal antibodies (MoAb) as for diagnosis. In 14 cases, the metaphases proved positive to the MoAb: in 4, the cells with abnormality had their origin defined; in other 4 the karyotype was normal preventing any identification; 6 cases had minimal abnormalities not visible through CM; and in two cases abnormal karyotypes were detected only in the cultures with GM-CSF. This study showed that CM is feasible in cases where evident numerical or structural chromosomal abnormalties are present.Este trabalho avaliou um método combinado (MC) para identificação simultânea da morfologia, cariótipo e imunofenótipo da célula leucêmica. Foram estudados 21 pacientes com leucemia aguda e 1 com LMC em crise blástica através da morfologia, citoquimica, imunofenotipagem e citogenética. O cariótipo foi feito em material proveniente da medula óssea pelas técnicas convencionais e para cada caso, foi feito o método direto (MD) e/ou três culturas. O MC consistia em separar pequena parte do material de qualquer cultura ou MD, preparar as laminas através de citocentrífuga e fazer a imunofenotipagem pelo método do APAAP com os mesmos anticorpos monoclonais (AcMo) usados ao diagnóstico. Em 14 casos as metáfases mostraram positividade para os AcMo: em 4 as células com anormalidades tiveram sua origem definida, em 4 casos o cariótipo era normal impedindo identificações e 6 tinham aberrações mínimas impossíveis de serem vistas através do MC. Em dois casos o cariótipo anormal foi encontrado apenas nas culturas com GM-CSF. O estudo mostrou que o MC é válido para casos com alterações cromossômicas numéricas ou estruturais evidentes.São Paulo Medical JournalSão Paulo Medical Journal1997-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicosapm.emnuvens.com.br/spmj/article/view/2083São Paulo Medical Journal; Vol. 115 No. 1 (1997); 1336-1342São Paulo Medical Journal; v. 115 n. 1 (1997); 1336-13421806-9460reponame:São Paulo medical journal (Online)instname:Associação Paulista de Medicinainstacron:APMenghttps://periodicosapm.emnuvens.com.br/spmj/article/view/2083/1980https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessChauffaille, Maria de Lourdes Lopes FerrariCoutinho, VicenteYamamoto, MihokoKerbauy, José2023-09-27T12:25:53Zoai:ojs.diagnosticoetratamento.emnuvens.com.br:article/2083Revistahttp://www.scielo.br/spmjPUBhttps://old.scielo.br/oai/scielo-oai.phprevistas@apm.org.br1806-94601516-3180opendoar:2023-09-27T12:25:53São Paulo medical journal (Online) - Associação Paulista de Medicinafalse |
dc.title.none.fl_str_mv |
Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias |
title |
Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias |
spellingShingle |
Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias Chauffaille, Maria de Lourdes Lopes Ferrari Leukemia chromosomes imunophenotype |
title_short |
Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias |
title_full |
Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias |
title_fullStr |
Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias |
title_full_unstemmed |
Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias |
title_sort |
Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias |
author |
Chauffaille, Maria de Lourdes Lopes Ferrari |
author_facet |
Chauffaille, Maria de Lourdes Lopes Ferrari Coutinho, Vicente Yamamoto, Mihoko Kerbauy, José |
author_role |
author |
author2 |
Coutinho, Vicente Yamamoto, Mihoko Kerbauy, José |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Chauffaille, Maria de Lourdes Lopes Ferrari Coutinho, Vicente Yamamoto, Mihoko Kerbauy, José |
dc.subject.por.fl_str_mv |
Leukemia chromosomes imunophenotype |
topic |
Leukemia chromosomes imunophenotype |
description |
In the present study, a combined method (CM) for attaining simultaneous identification of leukemic cell morphology, karyotype and immunophenotype has been evaluated in 21 patients with acute leukemia and 1 with CML in blast crisis were studied for morphology, citochemistry, immunophenotype and karyotype. Karyotype was performed in a bone marrow sample by using conventional techniques. In each case, direct method (DM) and/or three cultures were tried. The CM consisted in separating a small part of the material resulting from any of the cultures or DM, preparing slides through cytospin and immunophenotyping through APAAP method using the same monoclonal antibodies (MoAb) as for diagnosis. In 14 cases, the metaphases proved positive to the MoAb: in 4, the cells with abnormality had their origin defined; in other 4 the karyotype was normal preventing any identification; 6 cases had minimal abnormalities not visible through CM; and in two cases abnormal karyotypes were detected only in the cultures with GM-CSF. This study showed that CM is feasible in cases where evident numerical or structural chromosomal abnormalties are present. |
publishDate |
1997 |
dc.date.none.fl_str_mv |
1997-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicosapm.emnuvens.com.br/spmj/article/view/2083 |
url |
https://periodicosapm.emnuvens.com.br/spmj/article/view/2083 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicosapm.emnuvens.com.br/spmj/article/view/2083/1980 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
São Paulo Medical Journal São Paulo Medical Journal |
publisher.none.fl_str_mv |
São Paulo Medical Journal São Paulo Medical Journal |
dc.source.none.fl_str_mv |
São Paulo Medical Journal; Vol. 115 No. 1 (1997); 1336-1342 São Paulo Medical Journal; v. 115 n. 1 (1997); 1336-1342 1806-9460 reponame:São Paulo medical journal (Online) instname:Associação Paulista de Medicina instacron:APM |
instname_str |
Associação Paulista de Medicina |
instacron_str |
APM |
institution |
APM |
reponame_str |
São Paulo medical journal (Online) |
collection |
São Paulo medical journal (Online) |
repository.name.fl_str_mv |
São Paulo medical journal (Online) - Associação Paulista de Medicina |
repository.mail.fl_str_mv |
revistas@apm.org.br |
_version_ |
1825135071901777920 |