Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias

Detalhes bibliográficos
Autor(a) principal: Chauffaille, Maria de Lourdes Lopes Ferrari
Data de Publicação: 1997
Outros Autores: Coutinho, Vicente, Yamamoto, Mihoko, Kerbauy, José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: São Paulo medical journal (Online)
Texto Completo: https://periodicosapm.emnuvens.com.br/spmj/article/view/2083
Resumo: In the present study, a combined method (CM) for attaining simultaneous identification of leukemic cell morphology, karyotype and immunophenotype has been evaluated in 21 patients with acute leukemia and 1 with CML in blast crisis were studied for morphology, citochemistry, immunophenotype and karyotype. Karyotype was performed in a bone marrow sample by using conventional techniques. In each case, direct method (DM) and/or three cultures were tried. The CM consisted in separating a small part of the material resulting from any of the cultures or DM, preparing slides through cytospin and immunophenotyping through APAAP method using the same monoclonal antibodies (MoAb) as for diagnosis. In 14 cases, the metaphases proved positive to the MoAb: in 4, the cells with abnormality had their origin defined; in other 4 the karyotype was normal preventing any identification; 6 cases had minimal abnormalities not visible through CM; and in two cases abnormal karyotypes were detected only in the cultures with GM-CSF. This study showed that CM is feasible in cases where evident numerical or structural chromosomal abnormalties are present.
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spelling Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemiasLeukemiachromosomesimunophenotypeIn the present study, a combined method (CM) for attaining simultaneous identification of leukemic cell morphology, karyotype and immunophenotype has been evaluated in 21 patients with acute leukemia and 1 with CML in blast crisis were studied for morphology, citochemistry, immunophenotype and karyotype. Karyotype was performed in a bone marrow sample by using conventional techniques. In each case, direct method (DM) and/or three cultures were tried. The CM consisted in separating a small part of the material resulting from any of the cultures or DM, preparing slides through cytospin and immunophenotyping through APAAP method using the same monoclonal antibodies (MoAb) as for diagnosis. In 14 cases, the metaphases proved positive to the MoAb: in 4, the cells with abnormality had their origin defined; in other 4 the karyotype was normal preventing any identification; 6 cases had minimal abnormalities not visible through CM; and in two cases abnormal karyotypes were detected only in the cultures with GM-CSF. This study showed that CM is feasible in cases where evident numerical or structural chromosomal abnormalties are present.Este trabalho avaliou um método combinado (MC) para identificação simultânea da morfologia, cariótipo e imunofenótipo da célula leucêmica. Foram estudados 21 pacientes com leucemia aguda e 1 com LMC em crise blástica através da morfologia, citoquimica, imunofenotipagem e citogenética. O cariótipo foi feito em material proveniente da medula óssea pelas técnicas convencionais e para cada caso, foi feito o método direto (MD) e/ou três culturas. O MC consistia em separar pequena parte do material de qualquer cultura ou MD, preparar as laminas através de citocentrífuga e fazer a imunofenotipagem pelo método do APAAP com os mesmos anticorpos monoclonais (AcMo) usados ao diagnóstico. Em 14 casos as metáfases mostraram positividade para os AcMo: em 4 as células com anormalidades tiveram sua origem definida, em 4 casos o cariótipo era normal impedindo identificações e 6 tinham aberrações mínimas impossíveis de serem vistas através do MC. Em dois casos o cariótipo anormal foi encontrado apenas nas culturas com GM-CSF. O estudo mostrou que o MC é válido para casos com alterações cromossômicas numéricas ou estruturais evidentes.São Paulo Medical JournalSão Paulo Medical Journal1997-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicosapm.emnuvens.com.br/spmj/article/view/2083São Paulo Medical Journal; Vol. 115 No. 1 (1997); 1336-1342São Paulo Medical Journal; v. 115 n. 1 (1997); 1336-13421806-9460reponame:São Paulo medical journal (Online)instname:Associação Paulista de Medicinainstacron:APMenghttps://periodicosapm.emnuvens.com.br/spmj/article/view/2083/1980https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessChauffaille, Maria de Lourdes Lopes FerrariCoutinho, VicenteYamamoto, MihokoKerbauy, José2023-09-27T12:25:53Zoai:ojs.diagnosticoetratamento.emnuvens.com.br:article/2083Revistahttp://www.scielo.br/spmjPUBhttps://old.scielo.br/oai/scielo-oai.phprevistas@apm.org.br1806-94601516-3180opendoar:2023-09-27T12:25:53São Paulo medical journal (Online) - Associação Paulista de Medicinafalse
dc.title.none.fl_str_mv Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias
title Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias
spellingShingle Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias
Chauffaille, Maria de Lourdes Lopes Ferrari
Leukemia
chromosomes
imunophenotype
title_short Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias
title_full Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias
title_fullStr Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias
title_full_unstemmed Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias
title_sort Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias
author Chauffaille, Maria de Lourdes Lopes Ferrari
author_facet Chauffaille, Maria de Lourdes Lopes Ferrari
Coutinho, Vicente
Yamamoto, Mihoko
Kerbauy, José
author_role author
author2 Coutinho, Vicente
Yamamoto, Mihoko
Kerbauy, José
author2_role author
author
author
dc.contributor.author.fl_str_mv Chauffaille, Maria de Lourdes Lopes Ferrari
Coutinho, Vicente
Yamamoto, Mihoko
Kerbauy, José
dc.subject.por.fl_str_mv Leukemia
chromosomes
imunophenotype
topic Leukemia
chromosomes
imunophenotype
description In the present study, a combined method (CM) for attaining simultaneous identification of leukemic cell morphology, karyotype and immunophenotype has been evaluated in 21 patients with acute leukemia and 1 with CML in blast crisis were studied for morphology, citochemistry, immunophenotype and karyotype. Karyotype was performed in a bone marrow sample by using conventional techniques. In each case, direct method (DM) and/or three cultures were tried. The CM consisted in separating a small part of the material resulting from any of the cultures or DM, preparing slides through cytospin and immunophenotyping through APAAP method using the same monoclonal antibodies (MoAb) as for diagnosis. In 14 cases, the metaphases proved positive to the MoAb: in 4, the cells with abnormality had their origin defined; in other 4 the karyotype was normal preventing any identification; 6 cases had minimal abnormalities not visible through CM; and in two cases abnormal karyotypes were detected only in the cultures with GM-CSF. This study showed that CM is feasible in cases where evident numerical or structural chromosomal abnormalties are present.
publishDate 1997
dc.date.none.fl_str_mv 1997-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicosapm.emnuvens.com.br/spmj/article/view/2083
url https://periodicosapm.emnuvens.com.br/spmj/article/view/2083
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicosapm.emnuvens.com.br/spmj/article/view/2083/1980
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv São Paulo Medical Journal
São Paulo Medical Journal
publisher.none.fl_str_mv São Paulo Medical Journal
São Paulo Medical Journal
dc.source.none.fl_str_mv São Paulo Medical Journal; Vol. 115 No. 1 (1997); 1336-1342
São Paulo Medical Journal; v. 115 n. 1 (1997); 1336-1342
1806-9460
reponame:São Paulo medical journal (Online)
instname:Associação Paulista de Medicina
instacron:APM
instname_str Associação Paulista de Medicina
instacron_str APM
institution APM
reponame_str São Paulo medical journal (Online)
collection São Paulo medical journal (Online)
repository.name.fl_str_mv São Paulo medical journal (Online) - Associação Paulista de Medicina
repository.mail.fl_str_mv revistas@apm.org.br
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