Liquid chromatography/tandem mass spectrometry method to determine boldenone in bovine liver tissues
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | |
Tipo de documento: | Artigo |
Título da fonte: | Repositório da Produção Científica e Intelectual da Unicamp |
Texto Completo: | https://hdl.handle.net/20.500.12733/474 |
Resumo: | Abstract: Boldenone, an androgenic steroid, is forbidden for use in meat production in most countries worldwide. Residues of this drug in food present a potential risk to consumers. A sensitive LC/MS/MS method for analysis of 17 beta-boldenone using boldenone-d3 as an internal standard was developed. An enzymatic hydrolysis and extraction using ethyl acetate, methanol, and hexane were performed in the sample preparation. Parameters such as decision limit (CC alpha), detection capability (CC beta), precision, recovery, and ruggedness were evaluated according to the Brazilian Regulation 24/2009 (equivalent to European Union Decision 2002/657/EC) and International Organization for Standardization/International Electrotechnical Commission 17025:2005. CC alpha and CC beta were determined to be 0.17 and 0.29 mu g/kg, respectively. Average recoveries from bovine liver samples fortified with 1, 1.5, and 2 mu g/kg were around 100%. A complete statistical analysis was performed on the results obtained, including an estimation of the method uncertainty. The method is considered robust after being subjected to day-to-day analytical variations and has been used as a standard method in Brazil to report boldenone levels in bovine liver |
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Liquid chromatography/tandem mass spectrometry method to determine boldenone in bovine liver tissuesCromatografia líquida acoplada a espectrometria de massas sequencial (CL-EM-EM)Liquid chromatography coupled to tandem mass spectrometryBoldenone in bovine liver tissuesArtigo originalAbstract: Boldenone, an androgenic steroid, is forbidden for use in meat production in most countries worldwide. Residues of this drug in food present a potential risk to consumers. A sensitive LC/MS/MS method for analysis of 17 beta-boldenone using boldenone-d3 as an internal standard was developed. An enzymatic hydrolysis and extraction using ethyl acetate, methanol, and hexane were performed in the sample preparation. Parameters such as decision limit (CC alpha), detection capability (CC beta), precision, recovery, and ruggedness were evaluated according to the Brazilian Regulation 24/2009 (equivalent to European Union Decision 2002/657/EC) and International Organization for Standardization/International Electrotechnical Commission 17025:2005. CC alpha and CC beta were determined to be 0.17 and 0.29 mu g/kg, respectively. Average recoveries from bovine liver samples fortified with 1, 1.5, and 2 mu g/kg were around 100%. A complete statistical analysis was performed on the results obtained, including an estimation of the method uncertainty. The method is considered robust after being subjected to day-to-day analytical variations and has been used as a standard method in Brazil to report boldenone levels in bovine liverFechadoUNIVERSIDADE ESTADUAL DE CAMPINASSalerno, Alessandro GonzalesWanschel, Amarylis Claudine Bonito Azeredo2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12733/474SALERNO, Alessandro Gonzales; WANSCHEL, Amarylis Claudine Bonito Azeredo. Liquid chromatography/tandem mass spectrometry method to determine boldenone in bovine liver tissues. Journal of AOAC international. Cary, NC : Oxford University Press, 2014. Vol. 97, n. 5 (Sep./Oct.), p. 1476-1480. Disponível em: https://hdl.handle.net/20.500.12733/474. Acesso em: 24 mai. 2023.Inglêshttps://repositorio.unicamp.br/acervo/detalhe/1231642reponame:Repositório da Produção Científica e Intelectual da Unicampinstname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPinfo:eu-repo/semantics/openAccess2021-11-12T11:48:21Zoai:https://www.repositorio.unicamp.br/:1231642Repositório InstitucionalPUBhttp://repositorio.unicamp.br/oai/requestreposip@unicamp.bropendoar:2021-11-12T11:48:21Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP)false |
dc.title.none.fl_str_mv |
Liquid chromatography/tandem mass spectrometry method to determine boldenone in bovine liver tissues |
title |
Liquid chromatography/tandem mass spectrometry method to determine boldenone in bovine liver tissues |
spellingShingle |
Liquid chromatography/tandem mass spectrometry method to determine boldenone in bovine liver tissues Salerno, Alessandro Gonzales Cromatografia líquida acoplada a espectrometria de massas sequencial (CL-EM-EM) Liquid chromatography coupled to tandem mass spectrometry Boldenone in bovine liver tissues Artigo original |
title_short |
Liquid chromatography/tandem mass spectrometry method to determine boldenone in bovine liver tissues |
title_full |
Liquid chromatography/tandem mass spectrometry method to determine boldenone in bovine liver tissues |
title_fullStr |
Liquid chromatography/tandem mass spectrometry method to determine boldenone in bovine liver tissues |
title_full_unstemmed |
Liquid chromatography/tandem mass spectrometry method to determine boldenone in bovine liver tissues |
title_sort |
Liquid chromatography/tandem mass spectrometry method to determine boldenone in bovine liver tissues |
author |
Salerno, Alessandro Gonzales |
author_facet |
Salerno, Alessandro Gonzales Wanschel, Amarylis Claudine Bonito Azeredo |
author_role |
author |
author2 |
Wanschel, Amarylis Claudine Bonito Azeredo |
author2_role |
author |
dc.contributor.none.fl_str_mv |
UNIVERSIDADE ESTADUAL DE CAMPINAS |
dc.contributor.author.fl_str_mv |
Salerno, Alessandro Gonzales Wanschel, Amarylis Claudine Bonito Azeredo |
dc.subject.por.fl_str_mv |
Cromatografia líquida acoplada a espectrometria de massas sequencial (CL-EM-EM) Liquid chromatography coupled to tandem mass spectrometry Boldenone in bovine liver tissues Artigo original |
topic |
Cromatografia líquida acoplada a espectrometria de massas sequencial (CL-EM-EM) Liquid chromatography coupled to tandem mass spectrometry Boldenone in bovine liver tissues Artigo original |
description |
Abstract: Boldenone, an androgenic steroid, is forbidden for use in meat production in most countries worldwide. Residues of this drug in food present a potential risk to consumers. A sensitive LC/MS/MS method for analysis of 17 beta-boldenone using boldenone-d3 as an internal standard was developed. An enzymatic hydrolysis and extraction using ethyl acetate, methanol, and hexane were performed in the sample preparation. Parameters such as decision limit (CC alpha), detection capability (CC beta), precision, recovery, and ruggedness were evaluated according to the Brazilian Regulation 24/2009 (equivalent to European Union Decision 2002/657/EC) and International Organization for Standardization/International Electrotechnical Commission 17025:2005. CC alpha and CC beta were determined to be 0.17 and 0.29 mu g/kg, respectively. Average recoveries from bovine liver samples fortified with 1, 1.5, and 2 mu g/kg were around 100%. A complete statistical analysis was performed on the results obtained, including an estimation of the method uncertainty. The method is considered robust after being subjected to day-to-day analytical variations and has been used as a standard method in Brazil to report boldenone levels in bovine liver |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/20.500.12733/474 SALERNO, Alessandro Gonzales; WANSCHEL, Amarylis Claudine Bonito Azeredo. Liquid chromatography/tandem mass spectrometry method to determine boldenone in bovine liver tissues. Journal of AOAC international. Cary, NC : Oxford University Press, 2014. Vol. 97, n. 5 (Sep./Oct.), p. 1476-1480. Disponível em: https://hdl.handle.net/20.500.12733/474. Acesso em: 24 mai. 2023. |
url |
https://hdl.handle.net/20.500.12733/474 |
identifier_str_mv |
SALERNO, Alessandro Gonzales; WANSCHEL, Amarylis Claudine Bonito Azeredo. Liquid chromatography/tandem mass spectrometry method to determine boldenone in bovine liver tissues. Journal of AOAC international. Cary, NC : Oxford University Press, 2014. Vol. 97, n. 5 (Sep./Oct.), p. 1476-1480. Disponível em: https://hdl.handle.net/20.500.12733/474. Acesso em: 24 mai. 2023. |
dc.language.iso.fl_str_mv |
Inglês |
language_invalid_str_mv |
Inglês |
dc.relation.none.fl_str_mv |
https://repositorio.unicamp.br/acervo/detalhe/1231642 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório da Produção Científica e Intelectual da Unicamp instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
instname_str |
Universidade Estadual de Campinas (UNICAMP) |
instacron_str |
UNICAMP |
institution |
UNICAMP |
reponame_str |
Repositório da Produção Científica e Intelectual da Unicamp |
collection |
Repositório da Produção Científica e Intelectual da Unicamp |
repository.name.fl_str_mv |
Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP) |
repository.mail.fl_str_mv |
reposip@unicamp.br |
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