Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels.
Main Author: | |
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Publication Date: | 2010 |
Other Authors: | , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Download full: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/660121 |
Summary: | During earlier experiments, an SSR molecularmarker (176 SoyHSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean. After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the promoter region of Gmhsp17.6-L gene. It was also detected in this region that resistant genotypes presented insertions between AT(31) and AT(33) in size and susceptible genotypes, AT(9). Gmhsp17.6-L gene coding region presented a perfect match in amino acid sequence in all soybean genotypes. A ribonuclease protection assay showed that Gmhsp17.6-L gene mRNA transcripts were present in all genotypes. A real-time relative quantification (qPCR) indicated in the resistant individuals higher mRNA transcripts levels, which presented in the sequencing more AT(n) insertions. These results suggest that the number ofAT(n) insertions inside this promoter region couldmodulate up or down gene levels. Those findings can lead to the possibility of manipulating, between some limits, the mRNA transcripts levels using different sizes of AT(n) insertions. |
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Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels.Marcadores molecularesSoybeanNematóide de galhaMeloidogyne JavanicaNematóideSojaDuring earlier experiments, an SSR molecularmarker (176 SoyHSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean. After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the promoter region of Gmhsp17.6-L gene. It was also detected in this region that resistant genotypes presented insertions between AT(31) and AT(33) in size and susceptible genotypes, AT(9). Gmhsp17.6-L gene coding region presented a perfect match in amino acid sequence in all soybean genotypes. A ribonuclease protection assay showed that Gmhsp17.6-L gene mRNA transcripts were present in all genotypes. A real-time relative quantification (qPCR) indicated in the resistant individuals higher mRNA transcripts levels, which presented in the sequencing more AT(n) insertions. These results suggest that the number ofAT(n) insertions inside this promoter region couldmodulate up or down gene levels. Those findings can lead to the possibility of manipulating, between some limits, the mRNA transcripts levels using different sizes of AT(n) insertions.RENATA FUGANTI, UEM; MARIA DE FÁTIMA PIRES DA SILVA MACHADO, UEM; VALÉRIA STEFANIA LOPES, UNIFIL; JOAO FLAVIO VELOSO SILVA, CPAMT; CARLOS ALBERTO ARRABAL ARIAS, CNPSo; SILVANA REGINA ROCKENBACH MARIN, CNPSo; ELISEU BINNECK, CNPSo; RICARDO VILELA ABDELNOOR, CNPSo; FRANCISMAR CORREA MARCELINO, CNPSo; ALEXANDRE LIMA NEPOMUCENO, CNPSo.FUGANTI, R.MACHADO, M. de F. P. da S.LOPES, V. S.SILVA, J. F. V.ARIAS, C. A. A.MARIN, S. R. R.BINNECK, E.ABDELNOOR, R. V.MARCELINO, F. C.NEPOMUCENO, A. L.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2010-03-0820102017-08-03T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9 p.Journal of Biomedicine and Biotechnology, 2010.1110-7251http://www.alice.cnptia.embrapa.br/alice/handle/doc/66012110.1155/2010/847673enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:43:20Zoai:www.alice.cnptia.embrapa.br:doc/660121Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T02:43:20falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:43:20Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels. |
title |
Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels. |
spellingShingle |
Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels. FUGANTI, R. Marcadores moleculares Soybean Nematóide de galha Meloidogyne Javanica Nematóide Soja |
title_short |
Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels. |
title_full |
Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels. |
title_fullStr |
Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels. |
title_full_unstemmed |
Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels. |
title_sort |
Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels. |
author |
FUGANTI, R. |
author_facet |
FUGANTI, R. MACHADO, M. de F. P. da S. LOPES, V. S. SILVA, J. F. V. ARIAS, C. A. A. MARIN, S. R. R. BINNECK, E. ABDELNOOR, R. V. MARCELINO, F. C. NEPOMUCENO, A. L. |
author_role |
author |
author2 |
MACHADO, M. de F. P. da S. LOPES, V. S. SILVA, J. F. V. ARIAS, C. A. A. MARIN, S. R. R. BINNECK, E. ABDELNOOR, R. V. MARCELINO, F. C. NEPOMUCENO, A. L. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
RENATA FUGANTI, UEM; MARIA DE FÁTIMA PIRES DA SILVA MACHADO, UEM; VALÉRIA STEFANIA LOPES, UNIFIL; JOAO FLAVIO VELOSO SILVA, CPAMT; CARLOS ALBERTO ARRABAL ARIAS, CNPSo; SILVANA REGINA ROCKENBACH MARIN, CNPSo; ELISEU BINNECK, CNPSo; RICARDO VILELA ABDELNOOR, CNPSo; FRANCISMAR CORREA MARCELINO, CNPSo; ALEXANDRE LIMA NEPOMUCENO, CNPSo. |
dc.contributor.author.fl_str_mv |
FUGANTI, R. MACHADO, M. de F. P. da S. LOPES, V. S. SILVA, J. F. V. ARIAS, C. A. A. MARIN, S. R. R. BINNECK, E. ABDELNOOR, R. V. MARCELINO, F. C. NEPOMUCENO, A. L. |
dc.subject.por.fl_str_mv |
Marcadores moleculares Soybean Nematóide de galha Meloidogyne Javanica Nematóide Soja |
topic |
Marcadores moleculares Soybean Nematóide de galha Meloidogyne Javanica Nematóide Soja |
description |
During earlier experiments, an SSR molecularmarker (176 SoyHSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean. After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the promoter region of Gmhsp17.6-L gene. It was also detected in this region that resistant genotypes presented insertions between AT(31) and AT(33) in size and susceptible genotypes, AT(9). Gmhsp17.6-L gene coding region presented a perfect match in amino acid sequence in all soybean genotypes. A ribonuclease protection assay showed that Gmhsp17.6-L gene mRNA transcripts were present in all genotypes. A real-time relative quantification (qPCR) indicated in the resistant individuals higher mRNA transcripts levels, which presented in the sequencing more AT(n) insertions. These results suggest that the number ofAT(n) insertions inside this promoter region couldmodulate up or down gene levels. Those findings can lead to the possibility of manipulating, between some limits, the mRNA transcripts levels using different sizes of AT(n) insertions. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-03-08 2010 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2017-08-03T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of Biomedicine and Biotechnology, 2010. 1110-7251 http://www.alice.cnptia.embrapa.br/alice/handle/doc/660121 10.1155/2010/847673 |
identifier_str_mv |
Journal of Biomedicine and Biotechnology, 2010. 1110-7251 10.1155/2010/847673 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/660121 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 p. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503440164978688 |