Experimental Plasmodium vivax infection of key Anopheles species from the Brazilian Amazon

Detalhes bibliográficos
Autor(a) principal: Rios-Velásquez, Claudia M.
Data de Publicação: 2013
Outros Autores: Campos, Keillen M. Martins, Simões, Rejane C., Izzo, Thiago, Santos, Edineuza V. dos, Pessoa, Felipe A. C., Lima, José B. P., Monteiro, Wuelton M., Secundino, Náglia F. C., Lacerda, Marcus V. G., Tadei, Wanderli P., Pimenta, Paulo F. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11751
Resumo: Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
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spelling Rios-Velásquez, Claudia M.Campos, Keillen M. MartinsSimões, Rejane C.Izzo, ThiagoSantos, Edineuza V. dosPessoa, Felipe A. C.Lima, José B. P.Monteiro, Wuelton M.Secundino, Náglia F. C.Lacerda, Marcus V. G.Tadei, Wanderli P.Pimenta, Paulo F. P.2015-09-21T17:25:38Z2015-09-21T17:25:38Z2013RIOS-VELÁSQUEZ, Claudia M.; et al. Experimental Plasmodium vivax infection of key Anopheles species from the Brazilian Amazon. Malaria Journal, v.12, n.460, 10p, 2013.1475-2875https://www.arca.fiocruz.br/handle/icict/1175110.1186/1475-2875-12-460engBioMed CentralExperimental Plasmodium vivax infection of key Anopheles species from the Brazilian Amazoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Manaus, AM, Brasil.Instituto Nacional de Pesquisas da Amazônia, Manaus, AM, Brasil / Universidade do Estado do Amazonas. Manaus, AM, Brasil. / Ministerio da Saúde. Núcleo Amazonas. Fundação de Vigilância em Saúde. Manaus, AM, Brasil.Universidade Federal de Mato Grosso. Cuiabá, MT, Brasil.Instituto Nacional de Pesquisas da Amazônia. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Manaus, AM, Brasil.Instituto Nacional de Pesquisas da Amazônia. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou; Belo Horizonte, MG, Brasil.Background: Anopheles darlingi is the major malaria vector in countries located in the Amazon region. Anopheles aquasalis and Anopheles albitarsis s.l. are also proven vectors in this region. Anopheles nuneztovari s.l. and Anopheles triannulatus s.l. were found infected with Plasmodium vivax; however, their status as vectors is not yet well defined. Knowledge of susceptibility of Amazon anopheline populations to Plasmodium infection is necessary to better understand their vector capacity. Laboratory colonization of An. darlingi, the main Amazon vector, has proven to be difficult and presently An. aquasalis is the only available autonomous colony. Methods: Larvae of An. darlingi, An. albitarsis s.l., An. nuneztovari s.l. and An. triannulatus s.l. were collected in the field and reared until adult stage. Adults of An. aquasalis were obtained from a well-established colony. Mosquitoes were blood-fed using a membrane-feeding device containing infected blood from malarial patients. The infection of the distinct Anopheles species was evaluated by the impact variance of the following parameters: (a) parasitaemia density; (b) blood serum inactivation of the infective bloodmeal; (c) influence of gametocyte number on infection rates and number of oocysts. The goal of this work was to compare the susceptibility to P. vivax of four field-collected Anopheles species with colonized An. aquasalis. Results: All Anopheles species tested were susceptible to P. vivax infection, nevertheless the proportion of infected mosquitoes and the infection intensity measured by oocyst number varied significantly among species. Inactivation of the blood serum prior to mosquito feeding increased infection rates in An. darlingi and An. triannulatus s.l., but was diminished in An. albitarsis s.l. and An. aquasalis. There was a positive correlation between gametocyte density and the infection rate in all tests (Z = −8.37; p < 0.001) but varied among the mosquito species. Anopheles albitarsis s.l., An. aquasalis and An. nuneztovari s.l. had higher infection rates than An. darlingi. Conclusion: All field-collected Anopheles species, as well as colonized An. aquasalis are susceptible to experimental P. vivax infections by membrane feeding assays. Anopheles darlingi, An. albitarsis s.l. and An. aquasalis are very susceptible to P. vivax infection. However, colonized An. aquasalis mosquitoes showed the higher infection intensity represented by infection rate and oocyst numbers. This study is the first to characterize experimental development of Plasmodium infections in Amazon Anopheles vectors and also to endorse that P. vivax infection of colonized An. aquasalis is a feasible laboratory model.AnophelesMalariaMembrane feeding assayInfection rateOocystsAnophelesMalariaOocistosPlasmodium vivaxinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11751/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALjose_lima_etal_IOC_2013.pdfapplication/pdf530553https://www.arca.fiocruz.br/bitstream/icict/11751/2/jose_lima_etal_IOC_2013.pdfecc989c032bd9da39e398d263e88b3aeMD52TEXTjose_lima_etal_IOC_2013.pdf.txtjose_lima_etal_IOC_2013.pdf.txtExtracted texttext/plain53487https://www.arca.fiocruz.br/bitstream/icict/11751/3/jose_lima_etal_IOC_2013.pdf.txt5eca5af03ebd62e2fbcadf9b89df1eacMD53icict/117512022-06-24 13:10:44.239oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:10:44Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Experimental Plasmodium vivax infection of key Anopheles species from the Brazilian Amazon
title Experimental Plasmodium vivax infection of key Anopheles species from the Brazilian Amazon
spellingShingle Experimental Plasmodium vivax infection of key Anopheles species from the Brazilian Amazon
Rios-Velásquez, Claudia M.
Anopheles
Malaria
Membrane feeding assay
Infection rate
Oocysts
Anopheles
Malaria
Oocistos
Plasmodium vivax
title_short Experimental Plasmodium vivax infection of key Anopheles species from the Brazilian Amazon
title_full Experimental Plasmodium vivax infection of key Anopheles species from the Brazilian Amazon
title_fullStr Experimental Plasmodium vivax infection of key Anopheles species from the Brazilian Amazon
title_full_unstemmed Experimental Plasmodium vivax infection of key Anopheles species from the Brazilian Amazon
title_sort Experimental Plasmodium vivax infection of key Anopheles species from the Brazilian Amazon
author Rios-Velásquez, Claudia M.
author_facet Rios-Velásquez, Claudia M.
Campos, Keillen M. Martins
Simões, Rejane C.
Izzo, Thiago
Santos, Edineuza V. dos
Pessoa, Felipe A. C.
Lima, José B. P.
Monteiro, Wuelton M.
Secundino, Náglia F. C.
Lacerda, Marcus V. G.
Tadei, Wanderli P.
Pimenta, Paulo F. P.
author_role author
author2 Campos, Keillen M. Martins
Simões, Rejane C.
Izzo, Thiago
Santos, Edineuza V. dos
Pessoa, Felipe A. C.
Lima, José B. P.
Monteiro, Wuelton M.
Secundino, Náglia F. C.
Lacerda, Marcus V. G.
Tadei, Wanderli P.
Pimenta, Paulo F. P.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rios-Velásquez, Claudia M.
Campos, Keillen M. Martins
Simões, Rejane C.
Izzo, Thiago
Santos, Edineuza V. dos
Pessoa, Felipe A. C.
Lima, José B. P.
Monteiro, Wuelton M.
Secundino, Náglia F. C.
Lacerda, Marcus V. G.
Tadei, Wanderli P.
Pimenta, Paulo F. P.
dc.subject.en.pt_BR.fl_str_mv Anopheles
Malaria
Membrane feeding assay
Infection rate
Oocysts
topic Anopheles
Malaria
Membrane feeding assay
Infection rate
Oocysts
Anopheles
Malaria
Oocistos
Plasmodium vivax
dc.subject.decs.pt_BR.fl_str_mv Anopheles
Malaria
Oocistos
Plasmodium vivax
description Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-09-21T17:25:38Z
dc.date.available.fl_str_mv 2015-09-21T17:25:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RIOS-VELÁSQUEZ, Claudia M.; et al. Experimental Plasmodium vivax infection of key Anopheles species from the Brazilian Amazon. Malaria Journal, v.12, n.460, 10p, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11751
dc.identifier.issn.pt_BR.fl_str_mv 1475-2875
dc.identifier.doi.pt_BR.fl_str_mv 10.1186/1475-2875-12-460
identifier_str_mv RIOS-VELÁSQUEZ, Claudia M.; et al. Experimental Plasmodium vivax infection of key Anopheles species from the Brazilian Amazon. Malaria Journal, v.12, n.460, 10p, 2013.
1475-2875
10.1186/1475-2875-12-460
url https://www.arca.fiocruz.br/handle/icict/11751
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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