Improved ROS defense in the swimbladder of a facultative air-breathing erythrinid fish, jeju, compared to a non-air-breathing close relative, traira
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/15806 |
Resumo: | The jeju Hoplerythrinus unitaeniatus and the traira Hoplias malabaricus are two closely related erythrinid fish, both possessing a two-chambered physostomous swimbladder. In the jeju the anterior section of the posterior bladder is highly vascularized and the swimbladder is used for aerial respiration; the traira, in turn, is a water-breather that uses the swimbladder as a buoyancy organ and not for aerial oxygen uptake. Observation of the breathing behavior under different levels of water oxygenation revealed that the traira started aquatic surface respiration only under severe hypoxic conditions and did not breathe air. In the jeju air-breathing behavior was observed under normoxic conditions, and the frequency of air-breathing was significantly increased under hypoxic conditions. Unexpectedly, even under hyperoxic conditions (30 mg O2 L−1) the jeju continued to take air breaths, and compared with normoxic conditions the frequency was not reduced. Because the frequently air-exposed swimbladder tissue faces higher oxygen partial pressures than normally experienced by other fish tissues, it was hypothesized that in the facultative air-breathing jeju, swimbladder tissue would have a higher antioxidative capacity than the swimbladder tissue of the water breathing traira. Measurement of total glutathione (GSSG/GSH) concentration in anterior and posterior swimbladder tissue revealed a higher concentration of this antioxidant in swimbladder tissue as compared to muscle tissue in the jeju. Furthermore, the GSSG/GSH concentration in jeju tissues was significantly higher than in traira tissues. Similarly, activities of enzymes involved in the breakdown of reactive oxygen species were significantly higher in the jeju swimbladder as compared to the traira swimbladder. The results show that the jeju, using the swimbladder as an additional breathing organ, has an enhanced antioxidative capacity in the swimbladder as compared to the traira, using the swimbladder only as a buoyancy organ. © 2016, The Author(s). |
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Pelster, BerndGiacomin, Marina MussoiWood, Chris M.Val, Adalberto Luis2020-05-19T13:43:46Z2020-05-19T13:43:46Z2016https://repositorio.inpa.gov.br/handle/1/1580610.1007/s00360-016-0981-5The jeju Hoplerythrinus unitaeniatus and the traira Hoplias malabaricus are two closely related erythrinid fish, both possessing a two-chambered physostomous swimbladder. In the jeju the anterior section of the posterior bladder is highly vascularized and the swimbladder is used for aerial respiration; the traira, in turn, is a water-breather that uses the swimbladder as a buoyancy organ and not for aerial oxygen uptake. Observation of the breathing behavior under different levels of water oxygenation revealed that the traira started aquatic surface respiration only under severe hypoxic conditions and did not breathe air. In the jeju air-breathing behavior was observed under normoxic conditions, and the frequency of air-breathing was significantly increased under hypoxic conditions. Unexpectedly, even under hyperoxic conditions (30 mg O2 L−1) the jeju continued to take air breaths, and compared with normoxic conditions the frequency was not reduced. Because the frequently air-exposed swimbladder tissue faces higher oxygen partial pressures than normally experienced by other fish tissues, it was hypothesized that in the facultative air-breathing jeju, swimbladder tissue would have a higher antioxidative capacity than the swimbladder tissue of the water breathing traira. Measurement of total glutathione (GSSG/GSH) concentration in anterior and posterior swimbladder tissue revealed a higher concentration of this antioxidant in swimbladder tissue as compared to muscle tissue in the jeju. Furthermore, the GSSG/GSH concentration in jeju tissues was significantly higher than in traira tissues. Similarly, activities of enzymes involved in the breakdown of reactive oxygen species were significantly higher in the jeju swimbladder as compared to the traira swimbladder. The results show that the jeju, using the swimbladder as an additional breathing organ, has an enhanced antioxidative capacity in the swimbladder as compared to the traira, using the swimbladder only as a buoyancy organ. © 2016, The Author(s).Volume 186, Número 5, Pags. 615-624Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessFish ProteinGlutathioneGlutathione DisulfideOxidoreductaseReactive Oxygen MetaboliteAirAir SacAnimalsCharaciformesMetabolismMusclePhysiologyRespiratory FunctionAirAir SacsAnimalCharaciformesFish ProteinsGlutathioneGlutathione DisulfideMusclesOxidoreductasesReactive Oxygen SpeciesRespiratory Physiological PhenomenaImproved ROS defense in the swimbladder of a facultative air-breathing erythrinid fish, jeju, compared to a non-air-breathing close relative, trairainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiologyengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf1046416https://repositorio.inpa.gov.br/bitstream/1/15806/1/artigo-inpa.pdf1665e29eace763f78a6917689b0d5f8bMD511/158062020-05-19 10:22:03.89oai:repositorio:1/15806Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-19T14:22:03Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Improved ROS defense in the swimbladder of a facultative air-breathing erythrinid fish, jeju, compared to a non-air-breathing close relative, traira |
title |
Improved ROS defense in the swimbladder of a facultative air-breathing erythrinid fish, jeju, compared to a non-air-breathing close relative, traira |
spellingShingle |
Improved ROS defense in the swimbladder of a facultative air-breathing erythrinid fish, jeju, compared to a non-air-breathing close relative, traira Pelster, Bernd Fish Protein Glutathione Glutathione Disulfide Oxidoreductase Reactive Oxygen Metabolite Air Air Sac Animals Characiformes Metabolism Muscle Physiology Respiratory Function Air Air Sacs Animal Characiformes Fish Proteins Glutathione Glutathione Disulfide Muscles Oxidoreductases Reactive Oxygen Species Respiratory Physiological Phenomena |
title_short |
Improved ROS defense in the swimbladder of a facultative air-breathing erythrinid fish, jeju, compared to a non-air-breathing close relative, traira |
title_full |
Improved ROS defense in the swimbladder of a facultative air-breathing erythrinid fish, jeju, compared to a non-air-breathing close relative, traira |
title_fullStr |
Improved ROS defense in the swimbladder of a facultative air-breathing erythrinid fish, jeju, compared to a non-air-breathing close relative, traira |
title_full_unstemmed |
Improved ROS defense in the swimbladder of a facultative air-breathing erythrinid fish, jeju, compared to a non-air-breathing close relative, traira |
title_sort |
Improved ROS defense in the swimbladder of a facultative air-breathing erythrinid fish, jeju, compared to a non-air-breathing close relative, traira |
author |
Pelster, Bernd |
author_facet |
Pelster, Bernd Giacomin, Marina Mussoi Wood, Chris M. Val, Adalberto Luis |
author_role |
author |
author2 |
Giacomin, Marina Mussoi Wood, Chris M. Val, Adalberto Luis |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Pelster, Bernd Giacomin, Marina Mussoi Wood, Chris M. Val, Adalberto Luis |
dc.subject.eng.fl_str_mv |
Fish Protein Glutathione Glutathione Disulfide Oxidoreductase Reactive Oxygen Metabolite Air Air Sac Animals Characiformes Metabolism Muscle Physiology Respiratory Function Air Air Sacs Animal Characiformes Fish Proteins Glutathione Glutathione Disulfide Muscles Oxidoreductases Reactive Oxygen Species Respiratory Physiological Phenomena |
topic |
Fish Protein Glutathione Glutathione Disulfide Oxidoreductase Reactive Oxygen Metabolite Air Air Sac Animals Characiformes Metabolism Muscle Physiology Respiratory Function Air Air Sacs Animal Characiformes Fish Proteins Glutathione Glutathione Disulfide Muscles Oxidoreductases Reactive Oxygen Species Respiratory Physiological Phenomena |
description |
The jeju Hoplerythrinus unitaeniatus and the traira Hoplias malabaricus are two closely related erythrinid fish, both possessing a two-chambered physostomous swimbladder. In the jeju the anterior section of the posterior bladder is highly vascularized and the swimbladder is used for aerial respiration; the traira, in turn, is a water-breather that uses the swimbladder as a buoyancy organ and not for aerial oxygen uptake. Observation of the breathing behavior under different levels of water oxygenation revealed that the traira started aquatic surface respiration only under severe hypoxic conditions and did not breathe air. In the jeju air-breathing behavior was observed under normoxic conditions, and the frequency of air-breathing was significantly increased under hypoxic conditions. Unexpectedly, even under hyperoxic conditions (30 mg O2 L−1) the jeju continued to take air breaths, and compared with normoxic conditions the frequency was not reduced. Because the frequently air-exposed swimbladder tissue faces higher oxygen partial pressures than normally experienced by other fish tissues, it was hypothesized that in the facultative air-breathing jeju, swimbladder tissue would have a higher antioxidative capacity than the swimbladder tissue of the water breathing traira. Measurement of total glutathione (GSSG/GSH) concentration in anterior and posterior swimbladder tissue revealed a higher concentration of this antioxidant in swimbladder tissue as compared to muscle tissue in the jeju. Furthermore, the GSSG/GSH concentration in jeju tissues was significantly higher than in traira tissues. Similarly, activities of enzymes involved in the breakdown of reactive oxygen species were significantly higher in the jeju swimbladder as compared to the traira swimbladder. The results show that the jeju, using the swimbladder as an additional breathing organ, has an enhanced antioxidative capacity in the swimbladder as compared to the traira, using the swimbladder only as a buoyancy organ. © 2016, The Author(s). |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2020-05-19T13:43:46Z |
dc.date.available.fl_str_mv |
2020-05-19T13:43:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/15806 |
dc.identifier.doi.none.fl_str_mv |
10.1007/s00360-016-0981-5 |
url |
https://repositorio.inpa.gov.br/handle/1/15806 |
identifier_str_mv |
10.1007/s00360-016-0981-5 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 186, Número 5, Pags. 615-624 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology |
publisher.none.fl_str_mv |
Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
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Instituto Nacional de Pesquisas da Amazônia (INPA) |
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INPA |
institution |
INPA |
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Repositório Institucional do INPA |
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Repositório Institucional do INPA |
bitstream.url.fl_str_mv |
https://repositorio.inpa.gov.br/bitstream/1/15806/1/artigo-inpa.pdf |
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Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA) |
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