Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis

Bibliographic Details
Main Author: Almeida-Val, Vera Maria Fonseca
Publication Date: 2011
Other Authors: Oliveira, Alice Reis, Silva, Maria de Nazaré Paula da, Ferreira-Nozawa, Mônica Stropa, Araújo, Roziete Mendes, Val, Adalberto Luis, Nozawa, Sérgio Ricardo
Format: Article
Language: eng
Source: Repositório Institucional do INPA
Download full: https://repositorio.inpa.gov.br/handle/1/14955
Summary: Adaptation or acclimation to hypoxia occurs via the modulation of physiologically relevant genes, such aserythropoietin,transferrin, vascular endothelial growth factor, phosphofructokinase and lactate dehydrogenase A. In the present study, we have cloned, sequenced and examined the modulation of the LDH-A gene after an Amazonian fish species, Astronotus crassipinis(the Oscar), was exposed to hypoxia and anoxia. In earlier studies, we have discovered that adults of this species are extremely tolerant to hypoxia and anoxia, while the juveniles are less tolerant. Exposure of juveniles to acute hypoxia and anoxia resulted in increased LDH-A gene expression in skeletal and cardiac muscles. When exposed to graded hypoxia juveniles show decreased LDH-A expression. In adults, the levels of LDH-A mRNA did not increase in hypoxic or anoxic conditions. Our results demonstrate that, when given time for acclimation, fish at different life-stages are able to respond differently to survive hypoxic episodes. © 2011, Sociedade Brasileira de Genética.
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spelling Almeida-Val, Vera Maria FonsecaOliveira, Alice ReisSilva, Maria de Nazaré Paula daFerreira-Nozawa, Mônica StropaAraújo, Roziete MendesVal, Adalberto LuisNozawa, Sérgio Ricardo2020-05-07T13:59:06Z2020-05-07T13:59:06Z2011https://repositorio.inpa.gov.br/handle/1/1495510.1590/S1415-47572011000200025Adaptation or acclimation to hypoxia occurs via the modulation of physiologically relevant genes, such aserythropoietin,transferrin, vascular endothelial growth factor, phosphofructokinase and lactate dehydrogenase A. In the present study, we have cloned, sequenced and examined the modulation of the LDH-A gene after an Amazonian fish species, Astronotus crassipinis(the Oscar), was exposed to hypoxia and anoxia. In earlier studies, we have discovered that adults of this species are extremely tolerant to hypoxia and anoxia, while the juveniles are less tolerant. Exposure of juveniles to acute hypoxia and anoxia resulted in increased LDH-A gene expression in skeletal and cardiac muscles. When exposed to graded hypoxia juveniles show decreased LDH-A expression. In adults, the levels of LDH-A mRNA did not increase in hypoxic or anoxic conditions. Our results demonstrate that, when given time for acclimation, fish at different life-stages are able to respond differently to survive hypoxic episodes. © 2011, Sociedade Brasileira de Genética.Volume 34, Número 2, Pags. 315-322Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessLactate DehydrogenaseLactate Dehydrogenase Isoenzyme 5Rna, MessengerUnclassified DrugAcclimatizationAgeAnimals ExperimentAnimals TissueAnoxiaAstronotus CrassipinisBrasilControlled StudyFishGene ExpressionGene SequenceHeart MuscleHypoxiaLdh A GeneCloning, MolecularNonhumanNucleotide SequenceMuscle, SkeletalAstronotusPiscesAnoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Biologyengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf1808629https://repositorio.inpa.gov.br/bitstream/1/14955/1/artigo-inpa.pdfbf0e7be2dd857025bc15f2611fd2294fMD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/14955/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/149552020-07-14 10:29:50.476oai:repositorio:1/14955Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T14:29:50Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis
title Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis
spellingShingle Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis
Almeida-Val, Vera Maria Fonseca
Lactate Dehydrogenase
Lactate Dehydrogenase Isoenzyme 5
Rna, Messenger
Unclassified Drug
Acclimatization
Age
Animals Experiment
Animals Tissue
Anoxia
Astronotus Crassipinis
Brasil
Controlled Study
Fish
Gene Expression
Gene Sequence
Heart Muscle
Hypoxia
Ldh A Gene
Cloning, Molecular
Nonhuman
Nucleotide Sequence
Muscle, Skeletal
Astronotus
Pisces
title_short Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis
title_full Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis
title_fullStr Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis
title_full_unstemmed Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis
title_sort Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis
author Almeida-Val, Vera Maria Fonseca
author_facet Almeida-Val, Vera Maria Fonseca
Oliveira, Alice Reis
Silva, Maria de Nazaré Paula da
Ferreira-Nozawa, Mônica Stropa
Araújo, Roziete Mendes
Val, Adalberto Luis
Nozawa, Sérgio Ricardo
author_role author
author2 Oliveira, Alice Reis
Silva, Maria de Nazaré Paula da
Ferreira-Nozawa, Mônica Stropa
Araújo, Roziete Mendes
Val, Adalberto Luis
Nozawa, Sérgio Ricardo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida-Val, Vera Maria Fonseca
Oliveira, Alice Reis
Silva, Maria de Nazaré Paula da
Ferreira-Nozawa, Mônica Stropa
Araújo, Roziete Mendes
Val, Adalberto Luis
Nozawa, Sérgio Ricardo
dc.subject.eng.fl_str_mv Lactate Dehydrogenase
Lactate Dehydrogenase Isoenzyme 5
Rna, Messenger
Unclassified Drug
Acclimatization
Age
Animals Experiment
Animals Tissue
Anoxia
Astronotus Crassipinis
Brasil
Controlled Study
Fish
Gene Expression
Gene Sequence
Heart Muscle
Hypoxia
Ldh A Gene
Cloning, Molecular
Nonhuman
Nucleotide Sequence
Muscle, Skeletal
Astronotus
Pisces
topic Lactate Dehydrogenase
Lactate Dehydrogenase Isoenzyme 5
Rna, Messenger
Unclassified Drug
Acclimatization
Age
Animals Experiment
Animals Tissue
Anoxia
Astronotus Crassipinis
Brasil
Controlled Study
Fish
Gene Expression
Gene Sequence
Heart Muscle
Hypoxia
Ldh A Gene
Cloning, Molecular
Nonhuman
Nucleotide Sequence
Muscle, Skeletal
Astronotus
Pisces
description Adaptation or acclimation to hypoxia occurs via the modulation of physiologically relevant genes, such aserythropoietin,transferrin, vascular endothelial growth factor, phosphofructokinase and lactate dehydrogenase A. In the present study, we have cloned, sequenced and examined the modulation of the LDH-A gene after an Amazonian fish species, Astronotus crassipinis(the Oscar), was exposed to hypoxia and anoxia. In earlier studies, we have discovered that adults of this species are extremely tolerant to hypoxia and anoxia, while the juveniles are less tolerant. Exposure of juveniles to acute hypoxia and anoxia resulted in increased LDH-A gene expression in skeletal and cardiac muscles. When exposed to graded hypoxia juveniles show decreased LDH-A expression. In adults, the levels of LDH-A mRNA did not increase in hypoxic or anoxic conditions. Our results demonstrate that, when given time for acclimation, fish at different life-stages are able to respond differently to survive hypoxic episodes. © 2011, Sociedade Brasileira de Genética.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2020-05-07T13:59:06Z
dc.date.available.fl_str_mv 2020-05-07T13:59:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/14955
dc.identifier.doi.none.fl_str_mv 10.1590/S1415-47572011000200025
url https://repositorio.inpa.gov.br/handle/1/14955
identifier_str_mv 10.1590/S1415-47572011000200025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 34, Número 2, Pags. 315-322
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Genetics and Molecular Biology
publisher.none.fl_str_mv Genetics and Molecular Biology
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Repositório Institucional do INPA
collection Repositório Institucional do INPA
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