Ecofisiologia e biomarcadores de estresse oxidativo na caracteriza??o do ciclo de vida p?s-embrion?rio de uma esp?cie de peixe anual neotropical
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da PUC_RS |
Texto Completo: | http://tede2.pucrs.br/tede2/handle/tede/8731 |
Resumo: | Freshwater fish are considered one of the most threatened groups in the world, as aquatic ecosystems are currently facing constant interference from anthropic actions, mainly with the intensification of agriculture that has impacted and de-characterized diverse habitats of many species. To conserve biological diversity in these aquatic environments, it is extremely important to obtain information about the species that constitute these habitats. In this way, physiological tools can be used to characterized biological diversity and its ecological implications, and also include organisms responses to environmental changes and stressors. Annual fish are organisms that live in temporary wetlands that dry out seasonally. Due to this, the life cycle is considered short and fast presenting great potential as biological models for several researches, mainly those related to the study of aging. The aim of the present study was to analyze physiological markers related to the redox balance of the annual fish Cynopoecilus fulgens, endemic to southern Brazil, with environmental variables and estimates of the reproductive period. A total of 443 individuals were collected, including juveniles, adults and senile, between June to November 2016, around the Parque Nacional da Lagoa do Peixe, located in the Coastal Plain of Rio Grande do Sul, in three sampling units (P1, P2 and P3). These animals were euthanized in the field and in the laboratory were measured and weighed. After, they were homogenized for the Superoxide Dismutase, Catalase, Glutathione Peroxidase and Glutathione S-transferase assays, and for the determination of the levels of Lipoperoxidation, through TBARS, Carbonylated Proteins and Total Proteins. The results showed that males and females of C. fulgens use different physiological strategies of their redox balance during the life cycle, being influenced by the reproductive period and environmental variables (biotic and abiotic). Among the strategies, females show a great deal of investment in reproduction, especially in the period of sexual maturation and egg production, evidenced by the higher levels of TBARS when compared to males that seem to invest in somatic growth and body mass. In addition, the variables that had the greatest influence on the oxidative balance of these animals were: temperature, abundance of predator, abundance of annual fish Austrolebias minuano and abundance of C. fulgens. Also, the population of each temporary wetland presented different physiological responses to the adaptation to their life cycle, corroborating the influence of an environmental component as a modulator of this cycle. Additionally, it was observed that the animals of the pools P2 and P3 behave in a similar way among each other presented a longer cycle; suggesting that the antioxidant system of these animals presents high efficiency keeping the levels of lipoperoxidation and carbonylated proteins low. Fish of P1 have their shorter post-hatch life cycle possibly due to increased oxidative stress evidenced by increased lipoperoxidation. These results demonstrate that the use of Neotropical annual fish in the research is highly promising, allowing in-depth study on aging and toxicology testing. In addition, they emphasize the importance of developing environmental conservation strategies to preserve the characteristics of temporary wetlands, establishing these animals as sentinel species. |
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Oliveira, Guendalina Turcatohttp://lattes.cnpq.br/1189036200852586Lan?s, Luis Esteban Krausehttp://lattes.cnpq.br/4969483972915961http://lattes.cnpq.br/1021228742201107Castro, Bruna Dutra de2019-06-17T19:16:04Z2019-03-15http://tede2.pucrs.br/tede2/handle/tede/8731Freshwater fish are considered one of the most threatened groups in the world, as aquatic ecosystems are currently facing constant interference from anthropic actions, mainly with the intensification of agriculture that has impacted and de-characterized diverse habitats of many species. To conserve biological diversity in these aquatic environments, it is extremely important to obtain information about the species that constitute these habitats. In this way, physiological tools can be used to characterized biological diversity and its ecological implications, and also include organisms responses to environmental changes and stressors. Annual fish are organisms that live in temporary wetlands that dry out seasonally. Due to this, the life cycle is considered short and fast presenting great potential as biological models for several researches, mainly those related to the study of aging. The aim of the present study was to analyze physiological markers related to the redox balance of the annual fish Cynopoecilus fulgens, endemic to southern Brazil, with environmental variables and estimates of the reproductive period. A total of 443 individuals were collected, including juveniles, adults and senile, between June to November 2016, around the Parque Nacional da Lagoa do Peixe, located in the Coastal Plain of Rio Grande do Sul, in three sampling units (P1, P2 and P3). These animals were euthanized in the field and in the laboratory were measured and weighed. After, they were homogenized for the Superoxide Dismutase, Catalase, Glutathione Peroxidase and Glutathione S-transferase assays, and for the determination of the levels of Lipoperoxidation, through TBARS, Carbonylated Proteins and Total Proteins. The results showed that males and females of C. fulgens use different physiological strategies of their redox balance during the life cycle, being influenced by the reproductive period and environmental variables (biotic and abiotic). Among the strategies, females show a great deal of investment in reproduction, especially in the period of sexual maturation and egg production, evidenced by the higher levels of TBARS when compared to males that seem to invest in somatic growth and body mass. In addition, the variables that had the greatest influence on the oxidative balance of these animals were: temperature, abundance of predator, abundance of annual fish Austrolebias minuano and abundance of C. fulgens. Also, the population of each temporary wetland presented different physiological responses to the adaptation to their life cycle, corroborating the influence of an environmental component as a modulator of this cycle. Additionally, it was observed that the animals of the pools P2 and P3 behave in a similar way among each other presented a longer cycle; suggesting that the antioxidant system of these animals presents high efficiency keeping the levels of lipoperoxidation and carbonylated proteins low. Fish of P1 have their shorter post-hatch life cycle possibly due to increased oxidative stress evidenced by increased lipoperoxidation. These results demonstrate that the use of Neotropical annual fish in the research is highly promising, allowing in-depth study on aging and toxicology testing. In addition, they emphasize the importance of developing environmental conservation strategies to preserve the characteristics of temporary wetlands, establishing these animals as sentinel species.Os peixes de ?gua doce s?o considerados um dos grupos mais amea?ados do mundo, visto que os ecossistemas aqu?ticos atualmente est?o enfrentando constantes interfer?ncias de a??es antr?picas, principalmente, com a intensifica??o da agricultura que tem impactado e descaracterizado diversos habitats de muitas esp?cies. Frente a isto, para a conserva??o da diversidade biol?gica nesses ambientes aqu?ticos ? de extrema import?ncia obter informa??es sobre as esp?cies constituintes destes habitats. Desta forma, ferramentas fisiol?gicas podem ser utilizadas para caracterizar a diversidade biol?gica e suas implica??es ecol?gicas, e abranger tamb?m, as respostas dos organismos ?s altera??es ambientais e agentes estressores. Os peixes anuais s?o organismos que vivem em ?reas ?midas tempor?rias que secam sazonalmente. Devido a isto, apresentam um ciclo de vida curto e r?pido, mostrando um grande potencial para serem utilizados como modelos biol?gicos para diversas pesquisas, principalmente, aquelas ligadas ao estudo de envelhecimento. O objetivo do presente trabalho foi analisar marcadores fisiol?gicos relacionados ao balan?o redox do peixe anual Cynopoecilus fulgens, end?mico da regi?o sul do Brasil, com vari?veis ambientais e estimativas do per?odo reprodutivo. Foram coletados 443 indiv?duos compreendendo juvenis, adultos e senis, entre o m?s de junho e novembro de 2016, nos arredores do Parque Nacional da Lagoa do Peixe (PNLP), localizado na Plan?cie Costeira Externa do Rio Grande do Sul, em tr?s diferentes unidades amostrais (P1, P2 e P3). Estes animais foram eutanasiados em campo e em laborat?rio foram medidos (comprimento padr?o) e pesados (massa corporal). Ap?s, foram homogeneizados para a realiza??o dos ensaios da Super?xido Dismutase, Catalase, Glutationa Peroxidase e Glutationa S-transferase, e para determina??o dos n?veis de Lipoperoxida??o, atrav?s do TBARS, Prote?nas Carboniladas e Prote?nas Totais. Os resultados mostraram que machos e f?meas de C. fulgens empregam estrat?gias fisiol?gicas diferentes em seu balan?o redox durante o ciclo de vida, sendo influenciados pelo per?odo reprodutivo e vari?veis ambientais (bi?ticas e abi?ticas). Dentre as estrat?gias, as f?meas mostram investir bastante na reprodu??o, principalmente no per?odo de matura??o sexual e produ??o dos ovos, evidenciado pelos n?veis mais elevados de TBARS quando comparadas aos machos que parecem investir no crescimento som?tico e massa corporal. Al?m disso, as vari?veis que apresentaram maiores influ?ncias no balan?o oxidativo destes animais foram: temperatura, abund?ncia de predador, abund?ncia do peixe anual Austrolebias minuano e abund?ncia de C. fulgens. Tamb?m, a popula??o de cada ?rea ?mida tempor?ria apresenta respostas fisiol?gicas distintas frente ? adapta??o ao seu ciclo de vida, corroborando a influ?ncia de algum componente ambiental como modulador deste ciclo. Adicionalmente, foi observado que os animais das po?as P2 e P3 se comportam de maneira semelhante entre si apresentando um ciclo mais longo; sugerindo que o sistema antioxidante destes animais apresenta alta efici?ncia mantendo os n?veis de lipoperoxida??o e prote?nas carboniladas baixos. Enquanto os da P1 t?m seu ciclo de vida p?s-eclos?o mais curto possivelmente devido ao incremento do estresse oxidativo evidenciado pelo aumento da lipoperoxida??o. Estes resultados demonstram que a utiliza??o dos peixes anuais neotropicais na pesquisa ? altamente promissora, permitindo um estudo aprofundado sobre o envelhecimento e ensaio toxicol?gico. Al?m disso, enfatizam a import?ncia do desenvolvimento de estrat?gias de conserva??o ambiental para preservar as caracter?sticas das ?reas ?midas tempor?rias, estabelecendo estes animais como esp?cies sentinelas.Submitted by PPG Ecologia e Evolu??o da Biodiversidade (eebpg.ciencias@pucrs.br) on 2019-06-04T18:26:51Z No. of bitstreams: 1 DISSERTA??O - BRUNA DUTRA DE CASTRO.pdf: 1794562 bytes, checksum: 00c1201ab2e27b1bf0a431631a6ae202 (MD5)Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2019-06-17T18:54:45Z (GMT) No. of bitstreams: 1 DISSERTA??O - BRUNA DUTRA DE CASTRO.pdf: 1794562 bytes, checksum: 00c1201ab2e27b1bf0a431631a6ae202 (MD5)Made available in DSpace on 2019-06-17T19:16:04Z (GMT). No. of bitstreams: 1 DISSERTA??O - BRUNA DUTRA DE CASTRO.pdf: 1794562 bytes, checksum: 00c1201ab2e27b1bf0a431631a6ae202 (MD5) Previous issue date: 2019-03-15Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPqapplication/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/175629/DIS_BRUNA_DUTRA_DE_CASTRO_CONFIDENCIAL.pdf.jpghttp://tede2.pucrs.br:80/tede2/retrieve/181330/DIS_BRUNA_DUTRA_DE_CASTRO_COMPLETO.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s Gradua??o em Ecologia e Evolu??o da BiodiversidadePUCRSBrasilEscola de Ci?ncias?reas ?midas Tempor?riasBalan?o RedoxConserva??o AmbientalCynopoecilus FulgensEnvelhecimentoVari?veis AmbientaisAgingCynopoecilus FulgensEnvironmental ConservationEnvironmental VariablesRedox BalanceTemporary WetlandsCIENCIAS BIOLOGICAS::ZOOLOGIAEcofisiologia e biomarcadores de estresse oxidativo na caracteriza??o do ciclo de vida p?s-embrion?rio de uma esp?cie de peixe anual neotropicalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisTrabalho ser? publicado como artigo ou livro24 meses17/06/2021-64826523806012675585006001802873727776104890info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSORIGINALDIS_BRUNA_DUTRA_DE_CASTRO_COMPLETO.pdfDIS_BRUNA_DUTRA_DE_CASTRO_COMPLETO.pdfapplication/pdf1794562http://tede2.pucrs.br/tede2/bitstream/tede/8731/5/DIS_BRUNA_DUTRA_DE_CASTRO_COMPLETO.pdf00c1201ab2e27b1bf0a431631a6ae202MD55THUMBNAILDIS_BRUNA_DUTRA_DE_CASTRO_CONFIDENCIAL.pdf.jpgDIS_BRUNA_DUTRA_DE_CASTRO_CONFIDENCIAL.pdf.jpgimage/jpeg4085http://tede2.pucrs.br/tede2/bitstream/tede/8731/4/DIS_BRUNA_DUTRA_DE_CASTRO_CONFIDENCIAL.pdf.jpg24e76319f6e89a47684a6d5cffde7748MD54DIS_BRUNA_DUTRA_DE_CASTRO_COMPLETO.pdf.jpgDIS_BRUNA_DUTRA_DE_CASTRO_COMPLETO.pdf.jpgimage/jpeg5734http://tede2.pucrs.br/tede2/bitstream/tede/8731/7/DIS_BRUNA_DUTRA_DE_CASTRO_COMPLETO.pdf.jpgc0c9aefb8669dab9604cedd50186185cMD57TEXTDIS_BRUNA_DUTRA_DE_CASTRO_CONFIDENCIAL.pdf.txtDIS_BRUNA_DUTRA_DE_CASTRO_CONFIDENCIAL.pdf.txttext/plain1622http://tede2.pucrs.br/tede2/bitstream/tede/8731/3/DIS_BRUNA_DUTRA_DE_CASTRO_CONFIDENCIAL.pdf.txt63da7a468fb1b05615a587985e59ea95MD53DIS_BRUNA_DUTRA_DE_CASTRO_COMPLETO.pdf.txtDIS_BRUNA_DUTRA_DE_CASTRO_COMPLETO.pdf.txttext/plain170581http://tede2.pucrs.br/tede2/bitstream/tede/8731/6/DIS_BRUNA_DUTRA_DE_CASTRO_COMPLETO.pdf.txt330f9bffffbda7e2cd58476633d44331MD56LICENSElicense.txtlicense.txttext/plain; charset=utf-8590http://tede2.pucrs.br/tede2/bitstream/tede/8731/1/license.txt220e11f2d3ba5354f917c7035aadef24MD51tede/87312021-06-17 20:00:12.008oai:tede2.pucrs.br:tede/8731QXV0b3JpemE/P28gcGFyYSBQdWJsaWNhPz9vIEVsZXRyP25pY2E6IENvbSBiYXNlIG5vIGRpc3Bvc3RvIG5hIExlaSBGZWRlcmFsIG4/OS42MTAsIGRlIDE5IGRlIGZldmVyZWlybyBkZSAxOTk4LCBvIGF1dG9yIEFVVE9SSVpBIGEgcHVibGljYT8/byBlbGV0cj9uaWNhIGRhIHByZXNlbnRlIG9icmEgbm8gYWNlcnZvIGRhIEJpYmxpb3RlY2EgRGlnaXRhbCBkYSBQb250aWY/Y2lhIFVuaXZlcnNpZGFkZSBDYXQ/bGljYSBkbyBSaW8gR3JhbmRlIGRvIFN1bCwgc2VkaWFkYSBhIEF2LiBJcGlyYW5nYSA2NjgxLCBQb3J0byBBbGVncmUsIFJpbyBHcmFuZGUgZG8gU3VsLCBjb20gcmVnaXN0cm8gZGUgQ05QSiA4ODYzMDQxMzAwMDItODEgYmVtIGNvbW8gZW0gb3V0cmFzIGJpYmxpb3RlY2FzIGRpZ2l0YWlzLCBuYWNpb25haXMgZSBpbnRlcm5hY2lvbmFpcywgY29ucz9yY2lvcyBlIHJlZGVzID9zIHF1YWlzIGEgYmlibGlvdGVjYSBkYSBQVUNSUyBwb3NzYSBhIHZpciBwYXJ0aWNpcGFyLCBzZW0gP251cyBhbHVzaXZvIGFvcyBkaXJlaXRvcyBhdXRvcmFpcywgYSB0P3R1bG8gZGUgZGl2dWxnYT8/byBkYSBwcm9kdT8/byBjaWVudD9maWNhLgo=Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2021-06-17T23:00:12Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
Ecofisiologia e biomarcadores de estresse oxidativo na caracteriza??o do ciclo de vida p?s-embrion?rio de uma esp?cie de peixe anual neotropical |
title |
Ecofisiologia e biomarcadores de estresse oxidativo na caracteriza??o do ciclo de vida p?s-embrion?rio de uma esp?cie de peixe anual neotropical |
spellingShingle |
Ecofisiologia e biomarcadores de estresse oxidativo na caracteriza??o do ciclo de vida p?s-embrion?rio de uma esp?cie de peixe anual neotropical Castro, Bruna Dutra de ?reas ?midas Tempor?rias Balan?o Redox Conserva??o Ambiental Cynopoecilus Fulgens Envelhecimento Vari?veis Ambientais Aging Cynopoecilus Fulgens Environmental Conservation Environmental Variables Redox Balance Temporary Wetlands CIENCIAS BIOLOGICAS::ZOOLOGIA |
title_short |
Ecofisiologia e biomarcadores de estresse oxidativo na caracteriza??o do ciclo de vida p?s-embrion?rio de uma esp?cie de peixe anual neotropical |
title_full |
Ecofisiologia e biomarcadores de estresse oxidativo na caracteriza??o do ciclo de vida p?s-embrion?rio de uma esp?cie de peixe anual neotropical |
title_fullStr |
Ecofisiologia e biomarcadores de estresse oxidativo na caracteriza??o do ciclo de vida p?s-embrion?rio de uma esp?cie de peixe anual neotropical |
title_full_unstemmed |
Ecofisiologia e biomarcadores de estresse oxidativo na caracteriza??o do ciclo de vida p?s-embrion?rio de uma esp?cie de peixe anual neotropical |
title_sort |
Ecofisiologia e biomarcadores de estresse oxidativo na caracteriza??o do ciclo de vida p?s-embrion?rio de uma esp?cie de peixe anual neotropical |
author |
Castro, Bruna Dutra de |
author_facet |
Castro, Bruna Dutra de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Oliveira, Guendalina Turcato |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1189036200852586 |
dc.contributor.advisor-co1.fl_str_mv |
Lan?s, Luis Esteban Krause |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/4969483972915961 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1021228742201107 |
dc.contributor.author.fl_str_mv |
Castro, Bruna Dutra de |
contributor_str_mv |
Oliveira, Guendalina Turcato Lan?s, Luis Esteban Krause |
dc.subject.por.fl_str_mv |
?reas ?midas Tempor?rias Balan?o Redox Conserva??o Ambiental Cynopoecilus Fulgens Envelhecimento Vari?veis Ambientais |
topic |
?reas ?midas Tempor?rias Balan?o Redox Conserva??o Ambiental Cynopoecilus Fulgens Envelhecimento Vari?veis Ambientais Aging Cynopoecilus Fulgens Environmental Conservation Environmental Variables Redox Balance Temporary Wetlands CIENCIAS BIOLOGICAS::ZOOLOGIA |
dc.subject.eng.fl_str_mv |
Aging Cynopoecilus Fulgens Environmental Conservation Environmental Variables Redox Balance Temporary Wetlands |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::ZOOLOGIA |
description |
Freshwater fish are considered one of the most threatened groups in the world, as aquatic ecosystems are currently facing constant interference from anthropic actions, mainly with the intensification of agriculture that has impacted and de-characterized diverse habitats of many species. To conserve biological diversity in these aquatic environments, it is extremely important to obtain information about the species that constitute these habitats. In this way, physiological tools can be used to characterized biological diversity and its ecological implications, and also include organisms responses to environmental changes and stressors. Annual fish are organisms that live in temporary wetlands that dry out seasonally. Due to this, the life cycle is considered short and fast presenting great potential as biological models for several researches, mainly those related to the study of aging. The aim of the present study was to analyze physiological markers related to the redox balance of the annual fish Cynopoecilus fulgens, endemic to southern Brazil, with environmental variables and estimates of the reproductive period. A total of 443 individuals were collected, including juveniles, adults and senile, between June to November 2016, around the Parque Nacional da Lagoa do Peixe, located in the Coastal Plain of Rio Grande do Sul, in three sampling units (P1, P2 and P3). These animals were euthanized in the field and in the laboratory were measured and weighed. After, they were homogenized for the Superoxide Dismutase, Catalase, Glutathione Peroxidase and Glutathione S-transferase assays, and for the determination of the levels of Lipoperoxidation, through TBARS, Carbonylated Proteins and Total Proteins. The results showed that males and females of C. fulgens use different physiological strategies of their redox balance during the life cycle, being influenced by the reproductive period and environmental variables (biotic and abiotic). Among the strategies, females show a great deal of investment in reproduction, especially in the period of sexual maturation and egg production, evidenced by the higher levels of TBARS when compared to males that seem to invest in somatic growth and body mass. In addition, the variables that had the greatest influence on the oxidative balance of these animals were: temperature, abundance of predator, abundance of annual fish Austrolebias minuano and abundance of C. fulgens. Also, the population of each temporary wetland presented different physiological responses to the adaptation to their life cycle, corroborating the influence of an environmental component as a modulator of this cycle. Additionally, it was observed that the animals of the pools P2 and P3 behave in a similar way among each other presented a longer cycle; suggesting that the antioxidant system of these animals presents high efficiency keeping the levels of lipoperoxidation and carbonylated proteins low. Fish of P1 have their shorter post-hatch life cycle possibly due to increased oxidative stress evidenced by increased lipoperoxidation. These results demonstrate that the use of Neotropical annual fish in the research is highly promising, allowing in-depth study on aging and toxicology testing. In addition, they emphasize the importance of developing environmental conservation strategies to preserve the characteristics of temporary wetlands, establishing these animals as sentinel species. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-06-17T19:16:04Z |
dc.date.issued.fl_str_mv |
2019-03-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://tede2.pucrs.br/tede2/handle/tede/8731 |
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http://tede2.pucrs.br/tede2/handle/tede/8731 |
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por |
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por |
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-6482652380601267558 |
dc.relation.confidence.fl_str_mv |
500 600 |
dc.relation.sponsorship.fl_str_mv |
1802873727776104890 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
dc.publisher.program.fl_str_mv |
Programa de P?s Gradua??o em Ecologia e Evolu??o da Biodiversidade |
dc.publisher.initials.fl_str_mv |
PUCRS |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Escola de Ci?ncias |
publisher.none.fl_str_mv |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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