Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/15069 |
Resumo: | Geranium robertianum L. is used in folk medicine and herbalism practice for the treatment of a variety of ailments. Recently, we studied the bioactivity of several aqueous and organic extracts of this plant. In this work, the more active extracts were fractionated and the fractions evaluated for their antioxidant activity and cytotoxicity against several human tumor cell lines and non-tumor porcine liver primary cells. Some of the fractions from the acetone extract consistently displayed low EC 50 and GI 50 values and presented the higher contents of total phenolic compounds in comparison to other fractions. The phenolic compounds profile of the fractions was determined. The bio-guided fractionation of the extracts resulted in several fractions with improved bioactivity relative to the corresponding extracts. Their lower compositional complexity allowed the identification of more than two dozen compounds, to the best of our knowledge, so far not reported in G. robertianum. |
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Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activityGeranium robertianum L.FractionationAntioxidant activityAntiproliferative activityPhenolic compoundsGeranium robertianum L. is used in folk medicine and herbalism practice for the treatment of a variety of ailments. Recently, we studied the bioactivity of several aqueous and organic extracts of this plant. In this work, the more active extracts were fractionated and the fractions evaluated for their antioxidant activity and cytotoxicity against several human tumor cell lines and non-tumor porcine liver primary cells. Some of the fractions from the acetone extract consistently displayed low EC 50 and GI 50 values and presented the higher contents of total phenolic compounds in comparison to other fractions. The phenolic compounds profile of the fractions was determined. The bio-guided fractionation of the extracts resulted in several fractions with improved bioactivity relative to the corresponding extracts. Their lower compositional complexity allowed the identification of more than two dozen compounds, to the best of our knowledge, so far not reported in G. robertianum.This work was supported by European Investment Funds by FEDER/COMPETE/POCI- Operational Competitiveness and Internationalization Programme, under Project POCI-01-0145-FEDER-006958 and National Funds by FCT − Portuguese Foundation for Science and Technology under the projects UID/AGR/04033, UID/AGR/00690/2013 (CIMO) and UID/QUI/00616/2013 (CQ-VR). V.C. Graça, M.I. Dias and L. Barros thank FCT for SFRH/BD/52542/2014, SFRH/BD/84485/2012, and SFRH/BPD/107855/2015 grants, respectively.Biblioteca Digital do IPBGraça, Vânia C.Barros, LillianCalhelha, Ricardo C.Dias, Maria InêsFerreira, Isabel C.F.R.Santos, Paulo F.2018-01-25T10:00:00Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/15069engGraça, V.C.; Barros, Lillian; Calhelha, Ricardo C.; Dias, Maria Inês; Ferreira, Isabel C.F.R.; Santos, P.F. (2017). Bio-guided fractionation of extracts of Geranium robertianum L.: Relationship between phenolic profile and biological activity. Industrial Crops & Products. ISSN 0926-6690. 108, p. 543-5520926669010.1016/j.indcrop.2017.07.016info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:35:36Zoai:bibliotecadigital.ipb.pt:10198/15069Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:04:51.926853Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity |
title |
Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity |
spellingShingle |
Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity Graça, Vânia C. Geranium robertianum L. Fractionation Antioxidant activity Antiproliferative activity Phenolic compounds |
title_short |
Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity |
title_full |
Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity |
title_fullStr |
Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity |
title_full_unstemmed |
Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity |
title_sort |
Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity |
author |
Graça, Vânia C. |
author_facet |
Graça, Vânia C. Barros, Lillian Calhelha, Ricardo C. Dias, Maria Inês Ferreira, Isabel C.F.R. Santos, Paulo F. |
author_role |
author |
author2 |
Barros, Lillian Calhelha, Ricardo C. Dias, Maria Inês Ferreira, Isabel C.F.R. Santos, Paulo F. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Graça, Vânia C. Barros, Lillian Calhelha, Ricardo C. Dias, Maria Inês Ferreira, Isabel C.F.R. Santos, Paulo F. |
dc.subject.por.fl_str_mv |
Geranium robertianum L. Fractionation Antioxidant activity Antiproliferative activity Phenolic compounds |
topic |
Geranium robertianum L. Fractionation Antioxidant activity Antiproliferative activity Phenolic compounds |
description |
Geranium robertianum L. is used in folk medicine and herbalism practice for the treatment of a variety of ailments. Recently, we studied the bioactivity of several aqueous and organic extracts of this plant. In this work, the more active extracts were fractionated and the fractions evaluated for their antioxidant activity and cytotoxicity against several human tumor cell lines and non-tumor porcine liver primary cells. Some of the fractions from the acetone extract consistently displayed low EC 50 and GI 50 values and presented the higher contents of total phenolic compounds in comparison to other fractions. The phenolic compounds profile of the fractions was determined. The bio-guided fractionation of the extracts resulted in several fractions with improved bioactivity relative to the corresponding extracts. Their lower compositional complexity allowed the identification of more than two dozen compounds, to the best of our knowledge, so far not reported in G. robertianum. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z 2018-01-25T10:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/15069 |
url |
http://hdl.handle.net/10198/15069 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Graça, V.C.; Barros, Lillian; Calhelha, Ricardo C.; Dias, Maria Inês; Ferreira, Isabel C.F.R.; Santos, P.F. (2017). Bio-guided fractionation of extracts of Geranium robertianum L.: Relationship between phenolic profile and biological activity. Industrial Crops & Products. ISSN 0926-6690. 108, p. 543-552 09266690 10.1016/j.indcrop.2017.07.016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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