First cultivation and characterization of mycobacterium ulcerans from the environment

Detalhes bibliográficos
Autor(a) principal: Portaels, Françoise
Data de Publicação: 2008
Outros Autores: Meyers, Wayne M., Ablordey, Anthony, Castro, António G., Chemlal, Karim, Rijk, Pim de, Elsen, Pierre, Fissette, Krista, Fraga, Alexandra G., Lee, Richard, Mahrous, Engy, Small, Pamela L. C., Stragier, Pieter, Torrado, Egídio, Aerde, Anita Van, Silva, Manuel T., Pedrosa, Jorge
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/8728
Resumo: Background: Mycobacterium ulcerans disease, or Buruli ulcer (BU), is an indolent, necrotizing infection of skin, subcutaneous tissue and, occasionally, bones. It is the third most common human mycobacteriosis worldwide, after tuberculosis and leprosy. There is evidence that M. ulcerans is an environmental pathogen transmitted to humans from aquatic niches; however, well-characterized pure cultures of M. ulcerans from the environment have never been reported. Here we present details of the isolation and characterization of an M. ulcerans strain (00-1441) obtained from an aquatic Hemiptera (common name Water Strider, Gerris sp.) from Benin. Methodology/Principal Findings: One culture from a homogenate of a Gerris sp. in BACTEC became positive for IS2404, an insertion sequence with more than 200 copies in M. ulcerans. A pure culture of M. ulcerans 00-1441 was obtained on Lo¨ wenstein-Jensen medium after inoculation of BACTEC culture in mouse footpads followed by two other mouse footpad passages. The phenotypic characteristics of 00-1441 were identical to those of African M. ulcerans, including production of mycolactone A/B. The nucleotide sequence of the 59 end of 16S rRNA gene of 00-1441 was 100% identical to M. ulcerans and M. marinum, and the sequence of the 39 end was identical to that of the African type except for a single nucleotide substitution at position 1317. This mutation in M. ulcerans was recently discovered in BU patients living in the same geographic area. Various genotyping methods confirmed that strain 00-1441 has a profile identical to that of the predominant African type. Strain 00-1441 produced severe progressive infection and disease in mouse footpads with involvement of bone. Conclusion: Strain 00-1441 represents the first genetically and phenotypically identified strain of M. ulcerans isolated in pure culture from the environment. This isolation supports the concept that the agent of BU is a human pathogen with an environmental niche.
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spelling First cultivation and characterization of mycobacterium ulcerans from the environmentScience & TechnologyBackground: Mycobacterium ulcerans disease, or Buruli ulcer (BU), is an indolent, necrotizing infection of skin, subcutaneous tissue and, occasionally, bones. It is the third most common human mycobacteriosis worldwide, after tuberculosis and leprosy. There is evidence that M. ulcerans is an environmental pathogen transmitted to humans from aquatic niches; however, well-characterized pure cultures of M. ulcerans from the environment have never been reported. Here we present details of the isolation and characterization of an M. ulcerans strain (00-1441) obtained from an aquatic Hemiptera (common name Water Strider, Gerris sp.) from Benin. Methodology/Principal Findings: One culture from a homogenate of a Gerris sp. in BACTEC became positive for IS2404, an insertion sequence with more than 200 copies in M. ulcerans. A pure culture of M. ulcerans 00-1441 was obtained on Lo¨ wenstein-Jensen medium after inoculation of BACTEC culture in mouse footpads followed by two other mouse footpad passages. The phenotypic characteristics of 00-1441 were identical to those of African M. ulcerans, including production of mycolactone A/B. The nucleotide sequence of the 59 end of 16S rRNA gene of 00-1441 was 100% identical to M. ulcerans and M. marinum, and the sequence of the 39 end was identical to that of the African type except for a single nucleotide substitution at position 1317. This mutation in M. ulcerans was recently discovered in BU patients living in the same geographic area. Various genotyping methods confirmed that strain 00-1441 has a profile identical to that of the predominant African type. Strain 00-1441 produced severe progressive infection and disease in mouse footpads with involvement of bone. Conclusion: Strain 00-1441 represents the first genetically and phenotypically identified strain of M. ulcerans isolated in pure culture from the environment. This isolation supports the concept that the agent of BU is a human pathogen with an environmental niche.This work was partly supported by the Directorate-General for Development Cooperation (DGDC) Brussels (Belgium), the Fund for Scientific Research, Flanders (Belgium) (FWO grant nu G. 0301.01), by the Damien Foundation, Brussels (Belgium), by the European Commission, project nr. INCO-CT-2005-051476-BURULICO "Buruli ulcer: multidisciplinary research for improvement of control in Africa'', and by the National Institute of Allergy and Infectious Diseases, National Institutes of Health. This work was also supported by a grant from the Health Services of Fundacao Calouste Gulbenkian and by FCT fellowships Praxis SFRH/BD/9757/2003 and SFRH/BD/15911/2005 to E. Torrado and A. G. Fraga, respectively. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Public Library of ScienceUniversidade do MinhoPortaels, FrançoiseMeyers, Wayne M.Ablordey, AnthonyCastro, António G.Chemlal, KarimRijk, Pim deElsen, PierreFissette, KristaFraga, Alexandra G.Lee, RichardMahrous, EngySmall, Pamela L. C.Stragier, PieterTorrado, EgídioAerde, Anita VanSilva, Manuel T.Pedrosa, Jorge2008-032008-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/8728eng"PLOS Neglected Tropical Diseases." ISSN 1935-2735. 2:3 (Mar. 2008) 178.1935-273510.1371/journal.pntd.000017818365032http://www.plosntds.org/home.actioninfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:56:05Zoai:repositorium.sdum.uminho.pt:1822/8728Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:45:42.090654Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv First cultivation and characterization of mycobacterium ulcerans from the environment
title First cultivation and characterization of mycobacterium ulcerans from the environment
spellingShingle First cultivation and characterization of mycobacterium ulcerans from the environment
Portaels, Françoise
Science & Technology
title_short First cultivation and characterization of mycobacterium ulcerans from the environment
title_full First cultivation and characterization of mycobacterium ulcerans from the environment
title_fullStr First cultivation and characterization of mycobacterium ulcerans from the environment
title_full_unstemmed First cultivation and characterization of mycobacterium ulcerans from the environment
title_sort First cultivation and characterization of mycobacterium ulcerans from the environment
author Portaels, Françoise
author_facet Portaels, Françoise
Meyers, Wayne M.
Ablordey, Anthony
Castro, António G.
Chemlal, Karim
Rijk, Pim de
Elsen, Pierre
Fissette, Krista
Fraga, Alexandra G.
Lee, Richard
Mahrous, Engy
Small, Pamela L. C.
Stragier, Pieter
Torrado, Egídio
Aerde, Anita Van
Silva, Manuel T.
Pedrosa, Jorge
author_role author
author2 Meyers, Wayne M.
Ablordey, Anthony
Castro, António G.
Chemlal, Karim
Rijk, Pim de
Elsen, Pierre
Fissette, Krista
Fraga, Alexandra G.
Lee, Richard
Mahrous, Engy
Small, Pamela L. C.
Stragier, Pieter
Torrado, Egídio
Aerde, Anita Van
Silva, Manuel T.
Pedrosa, Jorge
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Portaels, Françoise
Meyers, Wayne M.
Ablordey, Anthony
Castro, António G.
Chemlal, Karim
Rijk, Pim de
Elsen, Pierre
Fissette, Krista
Fraga, Alexandra G.
Lee, Richard
Mahrous, Engy
Small, Pamela L. C.
Stragier, Pieter
Torrado, Egídio
Aerde, Anita Van
Silva, Manuel T.
Pedrosa, Jorge
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description Background: Mycobacterium ulcerans disease, or Buruli ulcer (BU), is an indolent, necrotizing infection of skin, subcutaneous tissue and, occasionally, bones. It is the third most common human mycobacteriosis worldwide, after tuberculosis and leprosy. There is evidence that M. ulcerans is an environmental pathogen transmitted to humans from aquatic niches; however, well-characterized pure cultures of M. ulcerans from the environment have never been reported. Here we present details of the isolation and characterization of an M. ulcerans strain (00-1441) obtained from an aquatic Hemiptera (common name Water Strider, Gerris sp.) from Benin. Methodology/Principal Findings: One culture from a homogenate of a Gerris sp. in BACTEC became positive for IS2404, an insertion sequence with more than 200 copies in M. ulcerans. A pure culture of M. ulcerans 00-1441 was obtained on Lo¨ wenstein-Jensen medium after inoculation of BACTEC culture in mouse footpads followed by two other mouse footpad passages. The phenotypic characteristics of 00-1441 were identical to those of African M. ulcerans, including production of mycolactone A/B. The nucleotide sequence of the 59 end of 16S rRNA gene of 00-1441 was 100% identical to M. ulcerans and M. marinum, and the sequence of the 39 end was identical to that of the African type except for a single nucleotide substitution at position 1317. This mutation in M. ulcerans was recently discovered in BU patients living in the same geographic area. Various genotyping methods confirmed that strain 00-1441 has a profile identical to that of the predominant African type. Strain 00-1441 produced severe progressive infection and disease in mouse footpads with involvement of bone. Conclusion: Strain 00-1441 represents the first genetically and phenotypically identified strain of M. ulcerans isolated in pure culture from the environment. This isolation supports the concept that the agent of BU is a human pathogen with an environmental niche.
publishDate 2008
dc.date.none.fl_str_mv 2008-03
2008-03-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/8728
url http://hdl.handle.net/1822/8728
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "PLOS Neglected Tropical Diseases." ISSN 1935-2735. 2:3 (Mar. 2008) 178.
1935-2735
10.1371/journal.pntd.0000178
18365032
http://www.plosntds.org/home.action
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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