Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000600203 |
Resumo: | Abstract Purpose To determine by histomorphometric analysis whether CO2 pneumoperitoneum interferes with collagen deposition in surgical wounds in the aponeurosis of rats. Methods This experiment involved 80 male Wistar rats, randomly allocated into four groups according to pneumoperitoneum period (PRE: 30 min preoperatively; POST: 30 min postoperatively; PP: 30 min pre- and postoperatively; C: control group). CO2 pneumoperitoneum was insufflated to 5 mmHg of pressure. A laparotomy was performed; 1 cm of the left colon was then resected, and an end-to-end anastomosis was performed to simulate surgical trauma, after which the abdominal wall was closed. On postoperative days 7 or 14, a sample of the abdominal wall was collected, stained with picrosirius red and observed under polarized light in an optical microscope. The amount of collagen was estimated by computerized histomorphometric analysis. Results There were no significant differences in collagen deposition between the control and experimental groups on postoperative days 7 (p=0.720) or 14 (p=0.933). The amount of collagen increased as expected in all groups between postoperative days 7 and 14 (p=0.0003). Conclusion At 5 mmHg, CO2 pneumoperitoneum does not interfere with collagen deposition in abdominal wall surgical wounds in rats. |
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Acta Cirúrgica Brasileira (Online) |
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Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?Wound HealingIncisional HerniaCollagenPneumoperitoneumLaparoscopyMinimally Invasive Surgical ProceduresRatsAbstract Purpose To determine by histomorphometric analysis whether CO2 pneumoperitoneum interferes with collagen deposition in surgical wounds in the aponeurosis of rats. Methods This experiment involved 80 male Wistar rats, randomly allocated into four groups according to pneumoperitoneum period (PRE: 30 min preoperatively; POST: 30 min postoperatively; PP: 30 min pre- and postoperatively; C: control group). CO2 pneumoperitoneum was insufflated to 5 mmHg of pressure. A laparotomy was performed; 1 cm of the left colon was then resected, and an end-to-end anastomosis was performed to simulate surgical trauma, after which the abdominal wall was closed. On postoperative days 7 or 14, a sample of the abdominal wall was collected, stained with picrosirius red and observed under polarized light in an optical microscope. The amount of collagen was estimated by computerized histomorphometric analysis. Results There were no significant differences in collagen deposition between the control and experimental groups on postoperative days 7 (p=0.720) or 14 (p=0.933). The amount of collagen increased as expected in all groups between postoperative days 7 and 14 (p=0.0003). Conclusion At 5 mmHg, CO2 pneumoperitoneum does not interfere with collagen deposition in abdominal wall surgical wounds in rats.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000600203Acta Cirúrgica Brasileira v.35 n.6 2020reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/s0102-865020200060000005info:eu-repo/semantics/openAccessMorais,Pedro Henrique Alves deSilva,Rafael Francisco AlvesRibeiro,Thiago da SilvaFarias,Igor Eduardo Caetano deLino Junior,Ruy de SouzaCarneiro,Fabiana PiraniDurães,Leonardo de CastroSousa,João Batista deeng2020-07-13T00:00:00Zoai:scielo:S0102-86502020000600203Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2020-07-13T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds? |
title |
Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds? |
spellingShingle |
Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds? Morais,Pedro Henrique Alves de Wound Healing Incisional Hernia Collagen Pneumoperitoneum Laparoscopy Minimally Invasive Surgical Procedures Rats |
title_short |
Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds? |
title_full |
Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds? |
title_fullStr |
Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds? |
title_full_unstemmed |
Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds? |
title_sort |
Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds? |
author |
Morais,Pedro Henrique Alves de |
author_facet |
Morais,Pedro Henrique Alves de Silva,Rafael Francisco Alves Ribeiro,Thiago da Silva Farias,Igor Eduardo Caetano de Lino Junior,Ruy de Souza Carneiro,Fabiana Pirani Durães,Leonardo de Castro Sousa,João Batista de |
author_role |
author |
author2 |
Silva,Rafael Francisco Alves Ribeiro,Thiago da Silva Farias,Igor Eduardo Caetano de Lino Junior,Ruy de Souza Carneiro,Fabiana Pirani Durães,Leonardo de Castro Sousa,João Batista de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Morais,Pedro Henrique Alves de Silva,Rafael Francisco Alves Ribeiro,Thiago da Silva Farias,Igor Eduardo Caetano de Lino Junior,Ruy de Souza Carneiro,Fabiana Pirani Durães,Leonardo de Castro Sousa,João Batista de |
dc.subject.por.fl_str_mv |
Wound Healing Incisional Hernia Collagen Pneumoperitoneum Laparoscopy Minimally Invasive Surgical Procedures Rats |
topic |
Wound Healing Incisional Hernia Collagen Pneumoperitoneum Laparoscopy Minimally Invasive Surgical Procedures Rats |
description |
Abstract Purpose To determine by histomorphometric analysis whether CO2 pneumoperitoneum interferes with collagen deposition in surgical wounds in the aponeurosis of rats. Methods This experiment involved 80 male Wistar rats, randomly allocated into four groups according to pneumoperitoneum period (PRE: 30 min preoperatively; POST: 30 min postoperatively; PP: 30 min pre- and postoperatively; C: control group). CO2 pneumoperitoneum was insufflated to 5 mmHg of pressure. A laparotomy was performed; 1 cm of the left colon was then resected, and an end-to-end anastomosis was performed to simulate surgical trauma, after which the abdominal wall was closed. On postoperative days 7 or 14, a sample of the abdominal wall was collected, stained with picrosirius red and observed under polarized light in an optical microscope. The amount of collagen was estimated by computerized histomorphometric analysis. Results There were no significant differences in collagen deposition between the control and experimental groups on postoperative days 7 (p=0.720) or 14 (p=0.933). The amount of collagen increased as expected in all groups between postoperative days 7 and 14 (p=0.0003). Conclusion At 5 mmHg, CO2 pneumoperitoneum does not interfere with collagen deposition in abdominal wall surgical wounds in rats. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000600203 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000600203 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s0102-865020200060000005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.35 n.6 2020 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
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1752126445810876416 |