Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?

Detalhes bibliográficos
Autor(a) principal: Morais,Pedro Henrique Alves de
Data de Publicação: 2020
Outros Autores: Silva,Rafael Francisco Alves, Ribeiro,Thiago da Silva, Farias,Igor Eduardo Caetano de, Lino Junior,Ruy de Souza, Carneiro,Fabiana Pirani, Durães,Leonardo de Castro, Sousa,João Batista de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000600203
Resumo: Abstract Purpose To determine by histomorphometric analysis whether CO2 pneumoperitoneum interferes with collagen deposition in surgical wounds in the aponeurosis of rats. Methods This experiment involved 80 male Wistar rats, randomly allocated into four groups according to pneumoperitoneum period (PRE: 30 min preoperatively; POST: 30 min postoperatively; PP: 30 min pre- and postoperatively; C: control group). CO2 pneumoperitoneum was insufflated to 5 mmHg of pressure. A laparotomy was performed; 1 cm of the left colon was then resected, and an end-to-end anastomosis was performed to simulate surgical trauma, after which the abdominal wall was closed. On postoperative days 7 or 14, a sample of the abdominal wall was collected, stained with picrosirius red and observed under polarized light in an optical microscope. The amount of collagen was estimated by computerized histomorphometric analysis. Results There were no significant differences in collagen deposition between the control and experimental groups on postoperative days 7 (p=0.720) or 14 (p=0.933). The amount of collagen increased as expected in all groups between postoperative days 7 and 14 (p=0.0003). Conclusion At 5 mmHg, CO2 pneumoperitoneum does not interfere with collagen deposition in abdominal wall surgical wounds in rats.
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spelling Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?Wound HealingIncisional HerniaCollagenPneumoperitoneumLaparoscopyMinimally Invasive Surgical ProceduresRatsAbstract Purpose To determine by histomorphometric analysis whether CO2 pneumoperitoneum interferes with collagen deposition in surgical wounds in the aponeurosis of rats. Methods This experiment involved 80 male Wistar rats, randomly allocated into four groups according to pneumoperitoneum period (PRE: 30 min preoperatively; POST: 30 min postoperatively; PP: 30 min pre- and postoperatively; C: control group). CO2 pneumoperitoneum was insufflated to 5 mmHg of pressure. A laparotomy was performed; 1 cm of the left colon was then resected, and an end-to-end anastomosis was performed to simulate surgical trauma, after which the abdominal wall was closed. On postoperative days 7 or 14, a sample of the abdominal wall was collected, stained with picrosirius red and observed under polarized light in an optical microscope. The amount of collagen was estimated by computerized histomorphometric analysis. Results There were no significant differences in collagen deposition between the control and experimental groups on postoperative days 7 (p=0.720) or 14 (p=0.933). The amount of collagen increased as expected in all groups between postoperative days 7 and 14 (p=0.0003). Conclusion At 5 mmHg, CO2 pneumoperitoneum does not interfere with collagen deposition in abdominal wall surgical wounds in rats.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000600203Acta Cirúrgica Brasileira v.35 n.6 2020reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/s0102-865020200060000005info:eu-repo/semantics/openAccessMorais,Pedro Henrique Alves deSilva,Rafael Francisco AlvesRibeiro,Thiago da SilvaFarias,Igor Eduardo Caetano deLino Junior,Ruy de SouzaCarneiro,Fabiana PiraniDurães,Leonardo de CastroSousa,João Batista deeng2020-07-13T00:00:00Zoai:scielo:S0102-86502020000600203Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2020-07-13T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?
title Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?
spellingShingle Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?
Morais,Pedro Henrique Alves de
Wound Healing
Incisional Hernia
Collagen
Pneumoperitoneum
Laparoscopy
Minimally Invasive Surgical Procedures
Rats
title_short Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?
title_full Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?
title_fullStr Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?
title_full_unstemmed Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?
title_sort Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?
author Morais,Pedro Henrique Alves de
author_facet Morais,Pedro Henrique Alves de
Silva,Rafael Francisco Alves
Ribeiro,Thiago da Silva
Farias,Igor Eduardo Caetano de
Lino Junior,Ruy de Souza
Carneiro,Fabiana Pirani
Durães,Leonardo de Castro
Sousa,João Batista de
author_role author
author2 Silva,Rafael Francisco Alves
Ribeiro,Thiago da Silva
Farias,Igor Eduardo Caetano de
Lino Junior,Ruy de Souza
Carneiro,Fabiana Pirani
Durães,Leonardo de Castro
Sousa,João Batista de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Morais,Pedro Henrique Alves de
Silva,Rafael Francisco Alves
Ribeiro,Thiago da Silva
Farias,Igor Eduardo Caetano de
Lino Junior,Ruy de Souza
Carneiro,Fabiana Pirani
Durães,Leonardo de Castro
Sousa,João Batista de
dc.subject.por.fl_str_mv Wound Healing
Incisional Hernia
Collagen
Pneumoperitoneum
Laparoscopy
Minimally Invasive Surgical Procedures
Rats
topic Wound Healing
Incisional Hernia
Collagen
Pneumoperitoneum
Laparoscopy
Minimally Invasive Surgical Procedures
Rats
description Abstract Purpose To determine by histomorphometric analysis whether CO2 pneumoperitoneum interferes with collagen deposition in surgical wounds in the aponeurosis of rats. Methods This experiment involved 80 male Wistar rats, randomly allocated into four groups according to pneumoperitoneum period (PRE: 30 min preoperatively; POST: 30 min postoperatively; PP: 30 min pre- and postoperatively; C: control group). CO2 pneumoperitoneum was insufflated to 5 mmHg of pressure. A laparotomy was performed; 1 cm of the left colon was then resected, and an end-to-end anastomosis was performed to simulate surgical trauma, after which the abdominal wall was closed. On postoperative days 7 or 14, a sample of the abdominal wall was collected, stained with picrosirius red and observed under polarized light in an optical microscope. The amount of collagen was estimated by computerized histomorphometric analysis. Results There were no significant differences in collagen deposition between the control and experimental groups on postoperative days 7 (p=0.720) or 14 (p=0.933). The amount of collagen increased as expected in all groups between postoperative days 7 and 14 (p=0.0003). Conclusion At 5 mmHg, CO2 pneumoperitoneum does not interfere with collagen deposition in abdominal wall surgical wounds in rats.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000600203
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000600203
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s0102-865020200060000005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.35 n.6 2020
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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