EVOLUÇÃO DIRIGIDA DE ENZIMAS: PEQUENAS MODIFICAÇÕES, MELHORES BIOCATALISADORES

Detalhes bibliográficos
Autor(a) principal: Teixeira,Iris S.
Data de Publicação: 2020
Outros Autores: Milagre,Cintia D. F.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000600773
Resumo: Biocatalysis is now a mature field, both in the laboratory and industrial scale. This approach counts on enzyme high selectivity, biodegradability, elegant control over the outcome of reaction conditions and as a possible solution to address some challenges in Green Chemistry faced by synthetic organic chemists. However, many wild-type ready-to-use enzymes were not designed to accommodate the organic substrates needed by today’s demands. Or they do not fit a predefined optimum process condition or even, useful reactions are not accessible because there is no enzyme counterpart for metallo- and organocatalysts. This review will give a brief introduction on the protein engineering tools (directed evolution, rational design, and semi-rational design) and will focus on directed evolution of enzymes, their impact in chemistry with examples from production of commodity chemicals to pharmaceutical intermediates and its potential as a tool to achieve greener chemistry criteria will be discussed.
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spelling EVOLUÇÃO DIRIGIDA DE ENZIMAS: PEQUENAS MODIFICAÇÕES, MELHORES BIOCATALISADORESprotein engineeringbiocatalysisgreen chemistryorganic synthesisBiocatalysis is now a mature field, both in the laboratory and industrial scale. This approach counts on enzyme high selectivity, biodegradability, elegant control over the outcome of reaction conditions and as a possible solution to address some challenges in Green Chemistry faced by synthetic organic chemists. However, many wild-type ready-to-use enzymes were not designed to accommodate the organic substrates needed by today’s demands. Or they do not fit a predefined optimum process condition or even, useful reactions are not accessible because there is no enzyme counterpart for metallo- and organocatalysts. This review will give a brief introduction on the protein engineering tools (directed evolution, rational design, and semi-rational design) and will focus on directed evolution of enzymes, their impact in chemistry with examples from production of commodity chemicals to pharmaceutical intermediates and its potential as a tool to achieve greener chemistry criteria will be discussed.Sociedade Brasileira de Química2020-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000600773Química Nova v.43 n.6 2020reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20170538info:eu-repo/semantics/openAccessTeixeira,Iris S.Milagre,Cintia D. F.por2020-07-20T00:00:00Zoai:scielo:S0100-40422020000600773Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2020-07-20T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv EVOLUÇÃO DIRIGIDA DE ENZIMAS: PEQUENAS MODIFICAÇÕES, MELHORES BIOCATALISADORES
title EVOLUÇÃO DIRIGIDA DE ENZIMAS: PEQUENAS MODIFICAÇÕES, MELHORES BIOCATALISADORES
spellingShingle EVOLUÇÃO DIRIGIDA DE ENZIMAS: PEQUENAS MODIFICAÇÕES, MELHORES BIOCATALISADORES
Teixeira,Iris S.
protein engineering
biocatalysis
green chemistry
organic synthesis
title_short EVOLUÇÃO DIRIGIDA DE ENZIMAS: PEQUENAS MODIFICAÇÕES, MELHORES BIOCATALISADORES
title_full EVOLUÇÃO DIRIGIDA DE ENZIMAS: PEQUENAS MODIFICAÇÕES, MELHORES BIOCATALISADORES
title_fullStr EVOLUÇÃO DIRIGIDA DE ENZIMAS: PEQUENAS MODIFICAÇÕES, MELHORES BIOCATALISADORES
title_full_unstemmed EVOLUÇÃO DIRIGIDA DE ENZIMAS: PEQUENAS MODIFICAÇÕES, MELHORES BIOCATALISADORES
title_sort EVOLUÇÃO DIRIGIDA DE ENZIMAS: PEQUENAS MODIFICAÇÕES, MELHORES BIOCATALISADORES
author Teixeira,Iris S.
author_facet Teixeira,Iris S.
Milagre,Cintia D. F.
author_role author
author2 Milagre,Cintia D. F.
author2_role author
dc.contributor.author.fl_str_mv Teixeira,Iris S.
Milagre,Cintia D. F.
dc.subject.por.fl_str_mv protein engineering
biocatalysis
green chemistry
organic synthesis
topic protein engineering
biocatalysis
green chemistry
organic synthesis
description Biocatalysis is now a mature field, both in the laboratory and industrial scale. This approach counts on enzyme high selectivity, biodegradability, elegant control over the outcome of reaction conditions and as a possible solution to address some challenges in Green Chemistry faced by synthetic organic chemists. However, many wild-type ready-to-use enzymes were not designed to accommodate the organic substrates needed by today’s demands. Or they do not fit a predefined optimum process condition or even, useful reactions are not accessible because there is no enzyme counterpart for metallo- and organocatalysts. This review will give a brief introduction on the protein engineering tools (directed evolution, rational design, and semi-rational design) and will focus on directed evolution of enzymes, their impact in chemistry with examples from production of commodity chemicals to pharmaceutical intermediates and its potential as a tool to achieve greener chemistry criteria will be discussed.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000600773
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000600773
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.21577/0100-4042.20170538
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.43 n.6 2020
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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