Adaptation of the tetrazolium test for evaluation of Ceiba speciosa seeds
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Publication Date: | 2011 |
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Format: | Article |
Language: | por |
Source: | Semina. Ciências Agrárias (Online) |
Download full: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4771 |
Summary: | The objective of this research was to define the tetrazolium test methodology for Ceiba speciosa seeds. Two C. speciosa seeds samples were used and, for each of these, it were determinate the moisture content and germination. For the tetrazolium test it were tested two periods of tetrazolium solution exposition (three and four hours), and three tetrazolium solutions concentrations (0.1, 0.5 and 1.0%). All the treatments were submitted to the temperature of 40°C in dark. The statistical design was completely randomized, in a factorial scheme 2x2x3 (samples x periods x concentrations) with four replications of 25 seeds. The means comparison was by the Duncan test at the 0.05 level of probability and were done correlation tests between tetrazolium and germination tests. Although the tetrazolium solution 0.1% allowed identify high percentages of viable seeds for both samples and periods, only the 0.5% concentration for four hours had a positive and significant correlation with the germination for both samples. Thus, the tetrazolium test for Ceiba speciosa seeds should be realized with the tetrazolium solution concentration of 0.5%, for four hours. |
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Semina. Ciências Agrárias (Online) |
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Adaptation of the tetrazolium test for evaluation of Ceiba speciosa seedsAdequação do teste de tetrazólio para avaliação da qualidade de sementes de Ceiba speciosaCeiba speciosaViabilityForest seeds.PaineiraViabilidadeSementes florestais.The objective of this research was to define the tetrazolium test methodology for Ceiba speciosa seeds. Two C. speciosa seeds samples were used and, for each of these, it were determinate the moisture content and germination. For the tetrazolium test it were tested two periods of tetrazolium solution exposition (three and four hours), and three tetrazolium solutions concentrations (0.1, 0.5 and 1.0%). All the treatments were submitted to the temperature of 40°C in dark. The statistical design was completely randomized, in a factorial scheme 2x2x3 (samples x periods x concentrations) with four replications of 25 seeds. The means comparison was by the Duncan test at the 0.05 level of probability and were done correlation tests between tetrazolium and germination tests. Although the tetrazolium solution 0.1% allowed identify high percentages of viable seeds for both samples and periods, only the 0.5% concentration for four hours had a positive and significant correlation with the germination for both samples. Thus, the tetrazolium test for Ceiba speciosa seeds should be realized with the tetrazolium solution concentration of 0.5%, for four hours. O objetivo deste estudo foi definir a metodologia mais adequada para a realização do teste de tetrazólio em sementes de Ceiba speciosa (A. St. - Hil.) Ravenna – Malvaceae (paineira). Duas amostras de sementes foram utilizadas, para as quais, inicialmente, determinaram-se o teor de água e a germinação. Para o teste de tetrazólio foram testados dois tempos de exposição, três e quatro horas, e três concentrações da solução de tetrazólio, 0,1, 0,5 e 1%. O delineamento estatístico foi inteiramente casualizado em esquema fatorial 2x2x3 (amostras x tempos x concentrações) com quatro repetições de 25 sementes. A comparação das médias foi feita pelo teste de Duncan a 5% e após foi realizada análise de correlação entre os resultados do tetrazólio e de germinação. Embora a concentração de 0,1% de tetrazólio tenha permitido a visualização de uma maior porcentagem de sementes viáveis, para ambas as amostras e ambos os tempos de exposição, apenas a concentração de 0,5% com quatro horas de exposição, se correlacionou positiva e significativamente com a germinação para ambas as amostras. Portanto, o teste de tetrazólio para sementes de paineira deve ser realizado utilizando-se para coloração a solução de tetrazólio a 0,5%, por quatro horas.UEL2011-10-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa empírica controladaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/477110.5433/1679-0359.2011v32n4p1243Semina: Ciências Agrárias; Vol. 32 No. 4 (2011); 1243-1250Semina: Ciências Agrárias; v. 32 n. 4 (2011); 1243-12501679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4771/8889Copyright (c) 2011 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessLazarotto, MaríliaPiveta, GrazielaMuniz, Marlove Fátima BriãoReiniger, Lia Rejane Silveira2023-01-27T15:40:40Zoai:ojs.pkp.sfu.ca:article/4771Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-27T15:40:40Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Adaptation of the tetrazolium test for evaluation of Ceiba speciosa seeds Adequação do teste de tetrazólio para avaliação da qualidade de sementes de Ceiba speciosa |
title |
Adaptation of the tetrazolium test for evaluation of Ceiba speciosa seeds |
spellingShingle |
Adaptation of the tetrazolium test for evaluation of Ceiba speciosa seeds Lazarotto, Marília Ceiba speciosa Viability Forest seeds. Paineira Viabilidade Sementes florestais. |
title_short |
Adaptation of the tetrazolium test for evaluation of Ceiba speciosa seeds |
title_full |
Adaptation of the tetrazolium test for evaluation of Ceiba speciosa seeds |
title_fullStr |
Adaptation of the tetrazolium test for evaluation of Ceiba speciosa seeds |
title_full_unstemmed |
Adaptation of the tetrazolium test for evaluation of Ceiba speciosa seeds |
title_sort |
Adaptation of the tetrazolium test for evaluation of Ceiba speciosa seeds |
author |
Lazarotto, Marília |
author_facet |
Lazarotto, Marília Piveta, Graziela Muniz, Marlove Fátima Brião Reiniger, Lia Rejane Silveira |
author_role |
author |
author2 |
Piveta, Graziela Muniz, Marlove Fátima Brião Reiniger, Lia Rejane Silveira |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Lazarotto, Marília Piveta, Graziela Muniz, Marlove Fátima Brião Reiniger, Lia Rejane Silveira |
dc.subject.por.fl_str_mv |
Ceiba speciosa Viability Forest seeds. Paineira Viabilidade Sementes florestais. |
topic |
Ceiba speciosa Viability Forest seeds. Paineira Viabilidade Sementes florestais. |
description |
The objective of this research was to define the tetrazolium test methodology for Ceiba speciosa seeds. Two C. speciosa seeds samples were used and, for each of these, it were determinate the moisture content and germination. For the tetrazolium test it were tested two periods of tetrazolium solution exposition (three and four hours), and three tetrazolium solutions concentrations (0.1, 0.5 and 1.0%). All the treatments were submitted to the temperature of 40°C in dark. The statistical design was completely randomized, in a factorial scheme 2x2x3 (samples x periods x concentrations) with four replications of 25 seeds. The means comparison was by the Duncan test at the 0.05 level of probability and were done correlation tests between tetrazolium and germination tests. Although the tetrazolium solution 0.1% allowed identify high percentages of viable seeds for both samples and periods, only the 0.5% concentration for four hours had a positive and significant correlation with the germination for both samples. Thus, the tetrazolium test for Ceiba speciosa seeds should be realized with the tetrazolium solution concentration of 0.5%, for four hours. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-10-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa empírica controlada |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4771 10.5433/1679-0359.2011v32n4p1243 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4771 |
identifier_str_mv |
10.5433/1679-0359.2011v32n4p1243 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4771/8889 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2011 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2011 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 32 No. 4 (2011); 1243-1250 Semina: Ciências Agrárias; v. 32 n. 4 (2011); 1243-1250 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306060896403456 |