Ornamental plant Pachystachys lutea as a source of promising endophytes for plant growth and phytoprotective activity
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/51737 |
Resumo: | Endophytes are growth-promoting agents capable of synthesizing phytohormones, uptaking nutrients, and controlling pathogens. There is a strong potential to exploit them in the agriculture field like biofertilizers and biocontrol agents. In this work, we aimed to evaluate endophytic fungi isolated from Pachystachys lutea for their potential to solubilize phosphate, synthesise indole acetic acid (IAA), antagonize phytopathogens, and promote plant growth under greenhouse conditions. The phosphate solubilization efficiency was assessed on Pikovskaya’s agar medium. For analysis of IAA production, mycelia plugs of endophytes were cultured in Potato Dextrose Broth medium supplemented with L-tryptophan, with Salkowski Reagent, and the absorbance of the culture was measured. The antagonism evaluation of strain Alternaria sp. PL75 against phytopathogens was performed using the paired-culture technique. The promotion of plant growth provided by Alternaria sp. PL75 was evaluated in tomato plants. All strains evaluated were able to solubilize phosphate; however, the strain Alternaria sp. PL75 was the most effective (4.29). Two strains, Nemania sp. PL27 and Alternaria sp. PL75, produced 1.86 and 1.73 μg mL-1 of IAA, respectively. In the antagonism assay, the endophyte Alternaria sp. PL75 and its fungal extract showed the best results against the pathogen Moniliophthora perniciosa. The greenhouse experiment result showed the endophyte Alternaria sp. PL75 increased the plantlets emergency speed index and the percentage of germination from 60 to 81.63%. It was also observed a statistical significance in the shoot length of the treated plants with the endophyte suspension (55.38 cm) compared to the control (41.67 cm). |
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Ornamental plant Pachystachys lutea as a source of promising endophytes for plant growth and phytoprotective activityOrnamental plant Pachystachys lutea as a source of promising endophytes for plant growth and phytoprotective activityphosphate solubilization; IAA; endophytic fungi; plant growth; antagonism.phosphate solubilization; IAA; endophytic fungi; plant growth; antagonism.Endophytes are growth-promoting agents capable of synthesizing phytohormones, uptaking nutrients, and controlling pathogens. There is a strong potential to exploit them in the agriculture field like biofertilizers and biocontrol agents. In this work, we aimed to evaluate endophytic fungi isolated from Pachystachys lutea for their potential to solubilize phosphate, synthesise indole acetic acid (IAA), antagonize phytopathogens, and promote plant growth under greenhouse conditions. The phosphate solubilization efficiency was assessed on Pikovskaya’s agar medium. For analysis of IAA production, mycelia plugs of endophytes were cultured in Potato Dextrose Broth medium supplemented with L-tryptophan, with Salkowski Reagent, and the absorbance of the culture was measured. The antagonism evaluation of strain Alternaria sp. PL75 against phytopathogens was performed using the paired-culture technique. The promotion of plant growth provided by Alternaria sp. PL75 was evaluated in tomato plants. All strains evaluated were able to solubilize phosphate; however, the strain Alternaria sp. PL75 was the most effective (4.29). Two strains, Nemania sp. PL27 and Alternaria sp. PL75, produced 1.86 and 1.73 μg mL-1 of IAA, respectively. In the antagonism assay, the endophyte Alternaria sp. PL75 and its fungal extract showed the best results against the pathogen Moniliophthora perniciosa. The greenhouse experiment result showed the endophyte Alternaria sp. PL75 increased the plantlets emergency speed index and the percentage of germination from 60 to 81.63%. It was also observed a statistical significance in the shoot length of the treated plants with the endophyte suspension (55.38 cm) compared to the control (41.67 cm).Endophytes are growth-promoting agents capable of synthesizing phytohormones, uptaking nutrients, and controlling pathogens. There is a strong potential to exploit them in the agriculture field like biofertilizers and biocontrol agents. In this work, we aimed to evaluate endophytic fungi isolated from Pachystachys lutea for their potential to solubilize phosphate, synthesise indole acetic acid (IAA), antagonize phytopathogens, and promote plant growth under greenhouse conditions. The phosphate solubilization efficiency was assessed on Pikovskaya’s agar medium. For analysis of IAA production, mycelia plugs of endophytes were cultured in Potato Dextrose Broth medium supplemented with L-tryptophan, with Salkowski Reagent, and the absorbance of the culture was measured. The antagonism evaluation of strain Alternaria sp. PL75 against phytopathogens was performed using the paired-culture technique. The promotion of plant growth provided by Alternaria sp. PL75 was evaluated in tomato plants. All strains evaluated were able to solubilize phosphate; however, the strain Alternaria sp. PL75 was the most effective (4.29). Two strains, Nemania sp. PL27 and Alternaria sp. PL75, produced 1.86 and 1.73 μg mL-1 of IAA, respectively. In the antagonism assay, the endophyte Alternaria sp. PL75 and its fungal extract showed the best results against the pathogen Moniliophthora perniciosa. The greenhouse experiment result showed the endophyte Alternaria sp. PL75 increased the plantlets emergency speed index and the percentage of germination from 60 to 81.63%. It was also observed a statistical significance in the shoot length of the treated plants with the endophyte suspension (55.38 cm) compared to the control (41.67 cm).Universidade Estadual De Maringá2021-03-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5173710.4025/actascibiolsci.v43i1.51737Acta Scientiarum. Biological Sciences; Vol 43 (2021): Publicação contínua; e51737Acta Scientiarum. Biological Sciences; v. 43 (2021): Publicação contínua; e517371807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/51737/751375151828Copyright (c) 2021 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRibeiro, Amanda da SilvaPolli, Andressa DomingosOliveira, AmandaOliveira, João Arthur dos Santos deEmmer, AndersonAlves, Leonardo HamamuraPereira, Omar Cléo NevesPamphile (In memorian), João Alencar2022-02-17T21:59:02Zoai:periodicos.uem.br/ojs:article/51737Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-17T21:59:02Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Ornamental plant Pachystachys lutea as a source of promising endophytes for plant growth and phytoprotective activity Ornamental plant Pachystachys lutea as a source of promising endophytes for plant growth and phytoprotective activity |
title |
Ornamental plant Pachystachys lutea as a source of promising endophytes for plant growth and phytoprotective activity |
spellingShingle |
Ornamental plant Pachystachys lutea as a source of promising endophytes for plant growth and phytoprotective activity Ribeiro, Amanda da Silva phosphate solubilization; IAA; endophytic fungi; plant growth; antagonism. phosphate solubilization; IAA; endophytic fungi; plant growth; antagonism. |
title_short |
Ornamental plant Pachystachys lutea as a source of promising endophytes for plant growth and phytoprotective activity |
title_full |
Ornamental plant Pachystachys lutea as a source of promising endophytes for plant growth and phytoprotective activity |
title_fullStr |
Ornamental plant Pachystachys lutea as a source of promising endophytes for plant growth and phytoprotective activity |
title_full_unstemmed |
Ornamental plant Pachystachys lutea as a source of promising endophytes for plant growth and phytoprotective activity |
title_sort |
Ornamental plant Pachystachys lutea as a source of promising endophytes for plant growth and phytoprotective activity |
author |
Ribeiro, Amanda da Silva |
author_facet |
Ribeiro, Amanda da Silva Polli, Andressa Domingos Oliveira, Amanda Oliveira, João Arthur dos Santos de Emmer, Anderson Alves, Leonardo Hamamura Pereira, Omar Cléo Neves Pamphile (In memorian), João Alencar |
author_role |
author |
author2 |
Polli, Andressa Domingos Oliveira, Amanda Oliveira, João Arthur dos Santos de Emmer, Anderson Alves, Leonardo Hamamura Pereira, Omar Cléo Neves Pamphile (In memorian), João Alencar |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Amanda da Silva Polli, Andressa Domingos Oliveira, Amanda Oliveira, João Arthur dos Santos de Emmer, Anderson Alves, Leonardo Hamamura Pereira, Omar Cléo Neves Pamphile (In memorian), João Alencar |
dc.subject.por.fl_str_mv |
phosphate solubilization; IAA; endophytic fungi; plant growth; antagonism. phosphate solubilization; IAA; endophytic fungi; plant growth; antagonism. |
topic |
phosphate solubilization; IAA; endophytic fungi; plant growth; antagonism. phosphate solubilization; IAA; endophytic fungi; plant growth; antagonism. |
description |
Endophytes are growth-promoting agents capable of synthesizing phytohormones, uptaking nutrients, and controlling pathogens. There is a strong potential to exploit them in the agriculture field like biofertilizers and biocontrol agents. In this work, we aimed to evaluate endophytic fungi isolated from Pachystachys lutea for their potential to solubilize phosphate, synthesise indole acetic acid (IAA), antagonize phytopathogens, and promote plant growth under greenhouse conditions. The phosphate solubilization efficiency was assessed on Pikovskaya’s agar medium. For analysis of IAA production, mycelia plugs of endophytes were cultured in Potato Dextrose Broth medium supplemented with L-tryptophan, with Salkowski Reagent, and the absorbance of the culture was measured. The antagonism evaluation of strain Alternaria sp. PL75 against phytopathogens was performed using the paired-culture technique. The promotion of plant growth provided by Alternaria sp. PL75 was evaluated in tomato plants. All strains evaluated were able to solubilize phosphate; however, the strain Alternaria sp. PL75 was the most effective (4.29). Two strains, Nemania sp. PL27 and Alternaria sp. PL75, produced 1.86 and 1.73 μg mL-1 of IAA, respectively. In the antagonism assay, the endophyte Alternaria sp. PL75 and its fungal extract showed the best results against the pathogen Moniliophthora perniciosa. The greenhouse experiment result showed the endophyte Alternaria sp. PL75 increased the plantlets emergency speed index and the percentage of germination from 60 to 81.63%. It was also observed a statistical significance in the shoot length of the treated plants with the endophyte suspension (55.38 cm) compared to the control (41.67 cm). |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03-23 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/51737 10.4025/actascibiolsci.v43i1.51737 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/51737 |
identifier_str_mv |
10.4025/actascibiolsci.v43i1.51737 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/51737/751375151828 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 43 (2021): Publicação contínua; e51737 Acta Scientiarum. Biological Sciences; v. 43 (2021): Publicação contínua; e51737 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
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1799317397821194240 |