Qualidade do sêmen equino criopreservado com L-acetil-cisteina
Main Author: | |
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Publication Date: | 2014 |
Format: | Master thesis |
Language: | por |
Source: | Repositório Institucional da UFG |
Download full: | http://repositorio.bc.ufg.br/tede/handle/tede/4034 |
Summary: | This study was conducted to evaluate the effect of adding L-acetyl-cysteine (CIS) as anti oxidant agent to extender used for sperm cryopreservation of equine Mangalarga Marchador. Three semen samples from seven healthy stallions were obtained by artificial vagina, diluted in commercial extender and distributed into two treatments: control without addition and CIS, containing the antioxidant concentration of 2.5 mM. After the stabilization period, the samples were filled into 0.5 mL straws and subjected to cryopreservation protocol manually. Sixty days after the samples were thawed and evaluated in vitro by means of computerized analysis of sperm kinetics (CASA) and the evaluation of the integrity of plasma and acrosomal membranes by fluorescent probes. Regarding the kinetic variables related to sperm was found that in samples supplemented with CIS percentage of total and progressive motility (TM) (MP) was higher (P <0.01) compared to control samples (23.95±3 , 33 X 11 .38±2.35 and 4.66 ± 0.84 X 2.04 ± 0.36, respectively), but variable for straightness (STR) and linearity (LIN) the percentages were highe r (p ≤ 0.01) for the control samples (86.95 ± 1.02 X 82.28 ± 1.02 and 49.85 ± 1.32 X 45.19 ± 1.17, respectively). As those related to the integrity of the membranes of the sperm variables was superior (P <0.05) in the number of sperm with intact acrosome reaction (ICRA) in the samples compared to those CIS Control (4.7 ± 1.6 x 0.8 ± 0.2, respectively). The results suggest that the addition of acetyl-L-cysteine concentration of 2.5 mM in the diluent medium before cryopreservation can improve both the motility as the integrity of the sperm plasma membrane equine Mangalarga Marcher. More studies should be done to confirm this effect in vivo. |
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Meirinhos, Maria Lúcia Gambarinihttp://lattes.cnpq.br/4440003524956701Viu, Marco AntonioOliveira, Rodrigo Arruda deMeirinhos, Maria Lúcia GambariniSantos, Klayto josé Gonçalves dosLopes, Dyomar Toledohttp://lattes.cnpq.br/7450465789196557Francisco Júnior, Arthur2015-01-30T13:36:00Z2014-08-27FRANCISCO JÚNIOR, Arthur. Qualidade do sêmen equino criopreservado com L-acetil-cisteina. 2014. 34 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2014.http://repositorio.bc.ufg.br/tede/handle/tede/4034This study was conducted to evaluate the effect of adding L-acetyl-cysteine (CIS) as anti oxidant agent to extender used for sperm cryopreservation of equine Mangalarga Marchador. Three semen samples from seven healthy stallions were obtained by artificial vagina, diluted in commercial extender and distributed into two treatments: control without addition and CIS, containing the antioxidant concentration of 2.5 mM. After the stabilization period, the samples were filled into 0.5 mL straws and subjected to cryopreservation protocol manually. Sixty days after the samples were thawed and evaluated in vitro by means of computerized analysis of sperm kinetics (CASA) and the evaluation of the integrity of plasma and acrosomal membranes by fluorescent probes. Regarding the kinetic variables related to sperm was found that in samples supplemented with CIS percentage of total and progressive motility (TM) (MP) was higher (P <0.01) compared to control samples (23.95±3 , 33 X 11 .38±2.35 and 4.66 ± 0.84 X 2.04 ± 0.36, respectively), but variable for straightness (STR) and linearity (LIN) the percentages were highe r (p ≤ 0.01) for the control samples (86.95 ± 1.02 X 82.28 ± 1.02 and 49.85 ± 1.32 X 45.19 ± 1.17, respectively). As those related to the integrity of the membranes of the sperm variables was superior (P <0.05) in the number of sperm with intact acrosome reaction (ICRA) in the samples compared to those CIS Control (4.7 ± 1.6 x 0.8 ± 0.2, respectively). The results suggest that the addition of acetyl-L-cysteine concentration of 2.5 mM in the diluent medium before cryopreservation can improve both the motility as the integrity of the sperm plasma membrane equine Mangalarga Marcher. More studies should be done to confirm this effect in vivo.Esse estudo foi conduzido com o objetivo de avaliar o efeito da adição de L-acetilcisteína (CIS) como agente anti oxidante ao meio diluente utilizado para criopreservação de espermatozóides de equinos da raça Mangalarga Marchador. Três amostras seminais de sete garanhões hígidos foram obtidas por meio de vagina artificial, diluídas em meio comercial e distribuídas em dois tratamentos: controle, sem adição e CIS, contendo o antioxidante na concentração de 2,5 mM. Após o período de estabilização as amostras foram envasadas em palhetas de 0,5 mL e submetidas ao protocolo de criopreservação manual. Sessenta dias após as amostras foram descongeladas e avaliadas in vitro por meio da análise computadorizada da cinética espermática (CASA) e da avaliação da inte gridade das membranas plasmática e acrosomal por meio de sondas fluorescentes. Em relação às variáveis relacionadas à cinética espermática verificou-se que nas amostras suplementadas com CIS o percentual de motilidade total (MT) e progressiva (MP) foi superior (P<0,01) em relação às amostras controle (23,95±3,33 X 11,38±2,35 e 4,66±0,84 X 2,04±0,36, respectivamente), mas para as variáveis retilinearidade (STR) e linearidade (LIN) os percentuais foram maiores (P≤0,01) para as amostras Controle (86,95±1,02 X 82,28±1,02 e 49,85±1,32 X 45,19±1,17, respectivamente). Quanto às variáveis relacionadas à integridade das membranas do espermatozóide houve superioridade (P<0,05) no número de espermatozóides íntegros com reação acrossomal (ICRA) nas amostras CIS em relação àquelas controle (4,7±1,6 X 0,8±0,2, respectivamente). Os resultados sugerem que a adição de L-acetil-cisteína na concentração de 2,5 mM ao meio diluente antes da criopreservação pode melhorar tanto a motilidade quanto a integridade da membrana plasmática de espermatozóides de equinos da raça Mangalarga Marchador. Mais estudos devem ser feitos para comprovar esse efeito in vivo.Submitted by Cássia Santos (cassia.bcufg@gmail.com) on 2015-01-30T11:04:36Z No. of bitstreams: 2 license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Dissertação - Arthur Francisco Júnior.pdf: 1488392 bytes, checksum: 48308d252d06dcc28ec7f91907c90eec (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-01-30T13:36:00Z (GMT) No. of bitstreams: 2 license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Dissertação - Arthur Francisco Júnior.pdf: 1488392 bytes, checksum: 48308d252d06dcc28ec7f91907c90eec (MD5)Made available in DSpace on 2015-01-30T13:36:00Z (GMT). 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dc.title.eng.fl_str_mv |
Qualidade do sêmen equino criopreservado com L-acetil-cisteina |
dc.title.alternative.eng.fl_str_mv |
Quality equine semen criopreserved with L-acteil-cisteina |
title |
Qualidade do sêmen equino criopreservado com L-acetil-cisteina |
spellingShingle |
Qualidade do sêmen equino criopreservado com L-acetil-cisteina Francisco Júnior, Arthur Espermatozóides Integridade de membranas Mangalarga marchador Motilidade Mangalarga marchador Membrane integrity Motility Sperm MEDICINA VETERINARIA::REPRODUCAO ANIMAL |
title_short |
Qualidade do sêmen equino criopreservado com L-acetil-cisteina |
title_full |
Qualidade do sêmen equino criopreservado com L-acetil-cisteina |
title_fullStr |
Qualidade do sêmen equino criopreservado com L-acetil-cisteina |
title_full_unstemmed |
Qualidade do sêmen equino criopreservado com L-acetil-cisteina |
title_sort |
Qualidade do sêmen equino criopreservado com L-acetil-cisteina |
author |
Francisco Júnior, Arthur |
author_facet |
Francisco Júnior, Arthur |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Meirinhos, Maria Lúcia Gambarini |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4440003524956701 |
dc.contributor.advisor-co1.fl_str_mv |
Viu, Marco Antonio |
dc.contributor.advisor-co2.fl_str_mv |
Oliveira, Rodrigo Arruda de |
dc.contributor.referee1.fl_str_mv |
Meirinhos, Maria Lúcia Gambarini |
dc.contributor.referee2.fl_str_mv |
Santos, Klayto josé Gonçalves dos |
dc.contributor.referee3.fl_str_mv |
Lopes, Dyomar Toledo |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7450465789196557 |
dc.contributor.author.fl_str_mv |
Francisco Júnior, Arthur |
contributor_str_mv |
Meirinhos, Maria Lúcia Gambarini Viu, Marco Antonio Oliveira, Rodrigo Arruda de Meirinhos, Maria Lúcia Gambarini Santos, Klayto josé Gonçalves dos Lopes, Dyomar Toledo |
dc.subject.por.fl_str_mv |
Espermatozóides Integridade de membranas Mangalarga marchador Motilidade |
topic |
Espermatozóides Integridade de membranas Mangalarga marchador Motilidade Mangalarga marchador Membrane integrity Motility Sperm MEDICINA VETERINARIA::REPRODUCAO ANIMAL |
dc.subject.eng.fl_str_mv |
Mangalarga marchador Membrane integrity Motility Sperm |
dc.subject.cnpq.fl_str_mv |
MEDICINA VETERINARIA::REPRODUCAO ANIMAL |
description |
This study was conducted to evaluate the effect of adding L-acetyl-cysteine (CIS) as anti oxidant agent to extender used for sperm cryopreservation of equine Mangalarga Marchador. Three semen samples from seven healthy stallions were obtained by artificial vagina, diluted in commercial extender and distributed into two treatments: control without addition and CIS, containing the antioxidant concentration of 2.5 mM. After the stabilization period, the samples were filled into 0.5 mL straws and subjected to cryopreservation protocol manually. Sixty days after the samples were thawed and evaluated in vitro by means of computerized analysis of sperm kinetics (CASA) and the evaluation of the integrity of plasma and acrosomal membranes by fluorescent probes. Regarding the kinetic variables related to sperm was found that in samples supplemented with CIS percentage of total and progressive motility (TM) (MP) was higher (P <0.01) compared to control samples (23.95±3 , 33 X 11 .38±2.35 and 4.66 ± 0.84 X 2.04 ± 0.36, respectively), but variable for straightness (STR) and linearity (LIN) the percentages were highe r (p ≤ 0.01) for the control samples (86.95 ± 1.02 X 82.28 ± 1.02 and 49.85 ± 1.32 X 45.19 ± 1.17, respectively). As those related to the integrity of the membranes of the sperm variables was superior (P <0.05) in the number of sperm with intact acrosome reaction (ICRA) in the samples compared to those CIS Control (4.7 ± 1.6 x 0.8 ± 0.2, respectively). The results suggest that the addition of acetyl-L-cysteine concentration of 2.5 mM in the diluent medium before cryopreservation can improve both the motility as the integrity of the sperm plasma membrane equine Mangalarga Marcher. More studies should be done to confirm this effect in vivo. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-08-27 |
dc.date.accessioned.fl_str_mv |
2015-01-30T13:36:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FRANCISCO JÚNIOR, Arthur. Qualidade do sêmen equino criopreservado com L-acetil-cisteina. 2014. 34 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/4034 |
identifier_str_mv |
FRANCISCO JÚNIOR, Arthur. Qualidade do sêmen equino criopreservado com L-acetil-cisteina. 2014. 34 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2014. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/4034 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
4581960685150189167 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-6217552114249094582 |
dc.relation.cnpq.fl_str_mv |
1098345750696262412 |
dc.relation.sponsorship.fl_str_mv |
-2555911436985713659 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Ciência Animal (EVZ) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Escola de Veterinária e Zootecnia - EVZ (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/494e83d7-0722-49c4-986a-f5b93da6808f/download http://repositorio.bc.ufg.br/tede/bitstreams/8e483979-6285-48cd-9b21-3ca08744e77d/download http://repositorio.bc.ufg.br/tede/bitstreams/78b940dc-e43f-4bfd-a16b-00143ac8b615/download http://repositorio.bc.ufg.br/tede/bitstreams/04f9ec06-6251-44ea-81b4-1de1d9a5de4b/download http://repositorio.bc.ufg.br/tede/bitstreams/ea3f5ae0-c68c-4154-b8b7-22e76c0b392d/download http://repositorio.bc.ufg.br/tede/bitstreams/3db5b761-3a2b-480d-b52a-d65dc6f6aba0/download http://repositorio.bc.ufg.br/tede/bitstreams/cb4e4c38-e778-42d1-9110-cd598c97f88b/download |
bitstream.checksum.fl_str_mv |
48308d252d06dcc28ec7f91907c90eec bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f 29b9d5e95be03707f9d4a2e110421c11 9da0b6dfac957114c6a7714714b86306 14634359697d7a17a8ddadb1e401cf20 cc73c4c239a4c332d642ba1e7c7a9fb2 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1798044404353597440 |