Atividade biológica e parâmetros bioquímicos e fisiológicos influenciados por fatores abióticos em Achillea millefolium L
Main Author: | |
---|---|
Publication Date: | 2013 |
Format: | Doctoral thesis |
Language: | por |
Source: | Repositório Institucional da UFPel - Guaiaca |
Download full: | http://repositorio.ufpel.edu.br/handle/ri/1996 |
Summary: | Several abiotic factors can coordinate or alter metabolic processes of growth and development plants such light, temperature, water availability, climate, soil nutrition and inorganic compounds. The objective of this study was to evaluate performance of A. millefolium L. (yarrow) against abiotic factors. To achieve this objective were have conducted two experiments in which the first was observed antimicrobial activity in vitro and essential oil yield of yarrow grown with nitrogen levels (0, 75 and 150 kg urea ha-1) and subjected different levels of shading 0% (full light) and 70% (70% shading). The second experiment were observed growth characteristics, production photosynthetic pigments, antioxidant enzymes activity, concentrations hydrogen peroxide, malondialdehyde, ascorbate and deidroascorbato, antioxidant activity by method DPPH and secondary metabolites synthesis in yarrow cultivated under light conditions (full light and blue, red and black shade net) and subjected different collection periods (eight weeks shading, two and four weeks after shading). The results show that oil yield extracted from flowers was stimulated by medium nitrogen level and full light increased the oil yield extracted from leaves. The highest essential oil yield was observed in reproductive structures. Antimicrobial activity of oil was confirmed against microorganisms species. Yeast strains were inhibited by the lowest concentration. The dry mass of leaves and flowers was favored in plants grown under full light. The content of chlorophyll a and b were higher in plants cultivated under blue net, total chlorophyll content was lower in plants under full light and plants two weeks after shading was increase of chlorophyll a, b and total, equivalent did not shaded plants. Catalase activity increased plants acclimated to high light intensity and low light did not stimulate ascorbate peroxidase, but increased superoxide dismutase and content of hydrogen peroxide. Lipid peroxidation was reduced presence of the spectrum blue and red light and low light intensity generated by nets black and blue, reduced accumulation of ascorbate. Content of deidroascorbato increased acclimation process to sunlight and antioxidant activity was more pronounced extracts of flowers. Levels total phenols and flavonoids were stimulated by full light. Oil yield extracted from flowers was higher than leaves. Major metabolite oil extracted from leaves was farnesol and flowers were farnesol and chamazulene. Results for medicinal species A. millefolium L. indicated that light intensity, light quality, collection periods and nitrogen fertilization influence growth, photosynthetic pigments production, secondary metabolites and antioxidant response. |
id |
UFPL_353f22f6c3340210f016770a0c606ea1 |
---|---|
oai_identifier_str |
oai:guaiaca.ufpel.edu.br:123456789/1996 |
network_acronym_str |
UFPL |
network_name_str |
Repositório Institucional da UFPel - Guaiaca |
repository_id_str |
|
spelling |
2014-08-20T13:59:06Z2013-05-242014-08-20T13:59:06Z2013-04-05LIMA, Milene Conceição. Biological activity and biochemical and physiological parameters influenced by abiotic factors in Achillea millefolium L.. 2013. 118 f. Tese (Doutorado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2013.http://repositorio.ufpel.edu.br/handle/ri/1996Several abiotic factors can coordinate or alter metabolic processes of growth and development plants such light, temperature, water availability, climate, soil nutrition and inorganic compounds. The objective of this study was to evaluate performance of A. millefolium L. (yarrow) against abiotic factors. To achieve this objective were have conducted two experiments in which the first was observed antimicrobial activity in vitro and essential oil yield of yarrow grown with nitrogen levels (0, 75 and 150 kg urea ha-1) and subjected different levels of shading 0% (full light) and 70% (70% shading). The second experiment were observed growth characteristics, production photosynthetic pigments, antioxidant enzymes activity, concentrations hydrogen peroxide, malondialdehyde, ascorbate and deidroascorbato, antioxidant activity by method DPPH and secondary metabolites synthesis in yarrow cultivated under light conditions (full light and blue, red and black shade net) and subjected different collection periods (eight weeks shading, two and four weeks after shading). The results show that oil yield extracted from flowers was stimulated by medium nitrogen level and full light increased the oil yield extracted from leaves. The highest essential oil yield was observed in reproductive structures. Antimicrobial activity of oil was confirmed against microorganisms species. Yeast strains were inhibited by the lowest concentration. The dry mass of leaves and flowers was favored in plants grown under full light. The content of chlorophyll a and b were higher in plants cultivated under blue net, total chlorophyll content was lower in plants under full light and plants two weeks after shading was increase of chlorophyll a, b and total, equivalent did not shaded plants. Catalase activity increased plants acclimated to high light intensity and low light did not stimulate ascorbate peroxidase, but increased superoxide dismutase and content of hydrogen peroxide. Lipid peroxidation was reduced presence of the spectrum blue and red light and low light intensity generated by nets black and blue, reduced accumulation of ascorbate. Content of deidroascorbato increased acclimation process to sunlight and antioxidant activity was more pronounced extracts of flowers. Levels total phenols and flavonoids were stimulated by full light. Oil yield extracted from flowers was higher than leaves. Major metabolite oil extracted from leaves was farnesol and flowers were farnesol and chamazulene. Results for medicinal species A. millefolium L. indicated that light intensity, light quality, collection periods and nitrogen fertilization influence growth, photosynthetic pigments production, secondary metabolites and antioxidant response.Diversos fatores abióticos podem coordenar ou alterar os processos metabólicos de crescimento e desenvolvimento em plantas como a luz, temperatura, disponibilidade hídrica, clima, nutrição do solo e compostos inorgânicos. O objetivo deste estudo foi avaliar o comportamento de A. millefolium L. (mil-folhas) frente aos fatores abióticos. Para atingir este objetivo foram realizados dois experimentos, na qual no primeiro foi verificada a atividade antimicrobiana in vitro e rendimento do óleo essencial de mil-folhas cultivada com concentrações de nitrogênio (0, 75 e 150 Kg ureia ha-1) e submetida a diferentes níveis de sombreamento 0% (luz plena) e 70% (sombreamento 70%). No segundo experimento foram avaliadas características de crescimento, produção de pigmentos fotossintéticos, atividade de enzimas antioxidantes, teores de peróxido de hidrogênio, malondialdeído, ascorbato e deidroascorbato, atividade antioxidante pelo método DPPH e síntese de metabólitos secundários em mil-folhas cultivada sob condições de luminosidade (luz plena e sombreamento com malha azul, vermelha e preta) e submetida a diferentes períodos de coleta (oito semanas de sombreamento, duas e quatro semanas após o sombreamento). Os principais resultados mostram que o rendimento do óleo extraído de flores foi estimulado pela concentração nitrogenada intermediária e o extraído de folhas foi favorecido pela luz plena, sendo maior nas estruturas reprodutivas. A atividade antimicrobiana do óleo foi confirmada frente aos microrganismos, sendo as cepas fúngicas inibidas pela menor concentração. A massa seca das folhas e flores foi favorecida nas plantas cultivadas com luz plena. Os teores de clorofila a e b foram maiores nas plantas cultivadas com a malha azul, o conteúdo de clorofila total foi menor nas plantas sob luz plena e em plantas com duas semanas após o sombreamento ocorreu um incremento de clorofila a, b e total, equivalendo-se às plantas não sombreadas. A atividade da catalase aumentou em plantas aclimatadas em alta intensidade de luz e a baixa luminosidade não estimulou a ascorbato peroxidase, mas incrementou a superóxido dismutase e o teor de peróxido de hidrogênio. A peroxidação lipídica foi reduzida na presença dos espectros de luz azul e vermelha e a baixa intensidade de luz, gerada pelas malhas preta e azul, reduziu o acúmulo de ascorbato. O conteúdo de deidroascorbato aumentou em processo de aclimatação à luz solar e a atividade antioxidante foi mais acentuada em extratos de flores. Os teores de fenois e flavonoides totais foram estimulados pela luz plena. O rendimento do óleo extraído de flores foi maior em relação ao de folhas. O metabólito majoritário do óleo extraído de folhas foi o farnesol e os extraídos de flores foram o farnesol e camazuleno. Os resultados encontrados para a espécie medicinal A. millefolium L. indicaram que a intensidade luminosa, qualidade de luz, períodos de coleta e fertilização nitrogenada influenciam no crescimento, produção de pigmentos fotossintéticos, de metabólitos secundários e resposta antioxidante.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em Fisiologia VegetalUFPelBRBiologiaMil-folhasQualidade de luzPeríodos de coletaConcentrações de nitrogênioTerpenoidesÓleo essencialYarrowLight qualityCollection periodsNitrogen levelsTerpenoidsEssential oilCNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETALAtividade biológica e parâmetros bioquímicos e fisiológicos influenciados por fatores abióticos em Achillea millefolium LBiological activity and biochemical and physiological parameters influenced by abiotic factors in Achillea millefolium L.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesishttp://lattes.cnpq.br/7741550959957775http://lattes.cnpq.br/2414061342279670Amarante, Luciano doLima, Milene Conceiçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALtese_milene_conceicao_lima.pdfapplication/pdf1117046http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1996/1/tese_milene_conceicao_lima.pdf61412e1bba4614df5d623d5245902770MD51open accessTEXTtese_milene_conceicao_lima.pdf.txttese_milene_conceicao_lima.pdf.txtExtracted Texttext/plain235445http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1996/2/tese_milene_conceicao_lima.pdf.txt01c9a6a8e48b54c7bbb4eb79e8a9e17eMD52open accessTHUMBNAILtese_milene_conceicao_lima.pdf.jpgtese_milene_conceicao_lima.pdf.jpgGenerated Thumbnailimage/jpeg1340http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1996/3/tese_milene_conceicao_lima.pdf.jpgf89c33fee44e73a56c948e6210b31ecfMD53open access123456789/19962019-08-19 12:01:49.793open accessoai:guaiaca.ufpel.edu.br:123456789/1996Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-08-19T15:01:49Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Atividade biológica e parâmetros bioquímicos e fisiológicos influenciados por fatores abióticos em Achillea millefolium L |
dc.title.alternative.eng.fl_str_mv |
Biological activity and biochemical and physiological parameters influenced by abiotic factors in Achillea millefolium L. |
title |
Atividade biológica e parâmetros bioquímicos e fisiológicos influenciados por fatores abióticos em Achillea millefolium L |
spellingShingle |
Atividade biológica e parâmetros bioquímicos e fisiológicos influenciados por fatores abióticos em Achillea millefolium L Lima, Milene Conceição Mil-folhas Qualidade de luz Períodos de coleta Concentrações de nitrogênio Terpenoides Óleo essencial Yarrow Light quality Collection periods Nitrogen levels Terpenoids Essential oil CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
title_short |
Atividade biológica e parâmetros bioquímicos e fisiológicos influenciados por fatores abióticos em Achillea millefolium L |
title_full |
Atividade biológica e parâmetros bioquímicos e fisiológicos influenciados por fatores abióticos em Achillea millefolium L |
title_fullStr |
Atividade biológica e parâmetros bioquímicos e fisiológicos influenciados por fatores abióticos em Achillea millefolium L |
title_full_unstemmed |
Atividade biológica e parâmetros bioquímicos e fisiológicos influenciados por fatores abióticos em Achillea millefolium L |
title_sort |
Atividade biológica e parâmetros bioquímicos e fisiológicos influenciados por fatores abióticos em Achillea millefolium L |
author |
Lima, Milene Conceição |
author_facet |
Lima, Milene Conceição |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/7741550959957775 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/2414061342279670 |
dc.contributor.advisor1.fl_str_mv |
Amarante, Luciano do |
dc.contributor.author.fl_str_mv |
Lima, Milene Conceição |
contributor_str_mv |
Amarante, Luciano do |
dc.subject.por.fl_str_mv |
Mil-folhas Qualidade de luz Períodos de coleta Concentrações de nitrogênio Terpenoides Óleo essencial |
topic |
Mil-folhas Qualidade de luz Períodos de coleta Concentrações de nitrogênio Terpenoides Óleo essencial Yarrow Light quality Collection periods Nitrogen levels Terpenoids Essential oil CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
dc.subject.eng.fl_str_mv |
Yarrow Light quality Collection periods Nitrogen levels Terpenoids Essential oil |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
description |
Several abiotic factors can coordinate or alter metabolic processes of growth and development plants such light, temperature, water availability, climate, soil nutrition and inorganic compounds. The objective of this study was to evaluate performance of A. millefolium L. (yarrow) against abiotic factors. To achieve this objective were have conducted two experiments in which the first was observed antimicrobial activity in vitro and essential oil yield of yarrow grown with nitrogen levels (0, 75 and 150 kg urea ha-1) and subjected different levels of shading 0% (full light) and 70% (70% shading). The second experiment were observed growth characteristics, production photosynthetic pigments, antioxidant enzymes activity, concentrations hydrogen peroxide, malondialdehyde, ascorbate and deidroascorbato, antioxidant activity by method DPPH and secondary metabolites synthesis in yarrow cultivated under light conditions (full light and blue, red and black shade net) and subjected different collection periods (eight weeks shading, two and four weeks after shading). The results show that oil yield extracted from flowers was stimulated by medium nitrogen level and full light increased the oil yield extracted from leaves. The highest essential oil yield was observed in reproductive structures. Antimicrobial activity of oil was confirmed against microorganisms species. Yeast strains were inhibited by the lowest concentration. The dry mass of leaves and flowers was favored in plants grown under full light. The content of chlorophyll a and b were higher in plants cultivated under blue net, total chlorophyll content was lower in plants under full light and plants two weeks after shading was increase of chlorophyll a, b and total, equivalent did not shaded plants. Catalase activity increased plants acclimated to high light intensity and low light did not stimulate ascorbate peroxidase, but increased superoxide dismutase and content of hydrogen peroxide. Lipid peroxidation was reduced presence of the spectrum blue and red light and low light intensity generated by nets black and blue, reduced accumulation of ascorbate. Content of deidroascorbato increased acclimation process to sunlight and antioxidant activity was more pronounced extracts of flowers. Levels total phenols and flavonoids were stimulated by full light. Oil yield extracted from flowers was higher than leaves. Major metabolite oil extracted from leaves was farnesol and flowers were farnesol and chamazulene. Results for medicinal species A. millefolium L. indicated that light intensity, light quality, collection periods and nitrogen fertilization influence growth, photosynthetic pigments production, secondary metabolites and antioxidant response. |
publishDate |
2013 |
dc.date.available.fl_str_mv |
2013-05-24 2014-08-20T13:59:06Z |
dc.date.issued.fl_str_mv |
2013-04-05 |
dc.date.accessioned.fl_str_mv |
2014-08-20T13:59:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LIMA, Milene Conceição. Biological activity and biochemical and physiological parameters influenced by abiotic factors in Achillea millefolium L.. 2013. 118 f. Tese (Doutorado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/1996 |
identifier_str_mv |
LIMA, Milene Conceição. Biological activity and biochemical and physiological parameters influenced by abiotic factors in Achillea millefolium L.. 2013. 118 f. Tese (Doutorado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2013. |
url |
http://repositorio.ufpel.edu.br/handle/ri/1996 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Fisiologia Vegetal |
dc.publisher.initials.fl_str_mv |
UFPel |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Biologia |
publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFPel - Guaiaca instname:Universidade Federal de Pelotas (UFPEL) instacron:UFPEL |
instname_str |
Universidade Federal de Pelotas (UFPEL) |
instacron_str |
UFPEL |
institution |
UFPEL |
reponame_str |
Repositório Institucional da UFPel - Guaiaca |
collection |
Repositório Institucional da UFPel - Guaiaca |
bitstream.url.fl_str_mv |
http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1996/1/tese_milene_conceicao_lima.pdf http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1996/2/tese_milene_conceicao_lima.pdf.txt http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1996/3/tese_milene_conceicao_lima.pdf.jpg |
bitstream.checksum.fl_str_mv |
61412e1bba4614df5d623d5245902770 01c9a6a8e48b54c7bbb4eb79e8a9e17e f89c33fee44e73a56c948e6210b31ecf |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL) |
repository.mail.fl_str_mv |
rippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.br |
_version_ |
1801846938553810944 |