Padroniza??o de um teste de soroaglutina??o macrosc?pica para diagn?stico da leptospirose em su?nos
Main Author: | |
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Publication Date: | 2008 |
Format: | Master thesis |
Language: | por |
Source: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
Download full: | https://tede.ufrrj.br/jspui/handle/tede/871 |
Summary: | Leptospirosis is considered a global health problem in human and veterinary medicine. It is a zoonotic disease caused by bacteria of the genus Leptospira, prevalent on every continent. The development of an effective macroscopic serum agglutination test against leptospirosis is justified, since the available ones are expensive, cumbersome and restricted to specialized laboratories. In Brazil, leptospirosis in pigs has been a major cause of reproductive failure in several states, mainly in the south and southeast regions of the country. Among the animals used in production of rural ecosystems, pigs have the most frequent clinical manifestations in the reproductive sphere with abortions, usually in the final third of pregnancy. Infertility, sterility or birth of debilitated or dead pigs which dies in the first days of life are signs of the presence of the bacteria in the breeding matrix. In the present study, six suspensions formulations of antigenic serovars pomona, icterohaemorrhagiae and copenhageni were used in single suspension form or in two serovars combinations. These were tested against pig serum with no clinical suspicion of leptospirosis compared with the microscopic agglutination test that is accepted as the gold standard by the World Health Organization (WHO). Among the six proposed suspensions the combination of serovars pomona + icterohaemorrhagiae showed the best result. The results demonstrates a percentage of 89% sensitivity and specificity of 89% for the suspension with the serovars combination of pomona + icterohaemorrhagiae and 87% for positive predictive value and 90% negative predictive value for the same combination, demonstrated by the statistics methods such as Chi-square, Kappa and Linear Logistic Model. Given the importance of leptospirosis in pigs and economic impact that it causes worldwide, in addition to the lack of current information, this study aimed to develop a screening rapid test for the diagnosis of pigs kept in variable dimension farms from rural regions of the Rio de Janeiro state. |
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Flausino, Walter368.511.757-20http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788774D0737.338.307-68http://lattes.cnpq.br/7334395107841698Lima, Gerson Silva de2016-04-28T20:17:28Z2009-02-032008-10-03LIMA, Gerson Silva de. Padroniza??o de um teste de soroaglutina??o macrosc?pica para diagn?stico da Leptospirose em su?nos. 2008. 97 f. Disserta??o (Mestrado em Microbiologia Veterin?ria) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2008.https://tede.ufrrj.br/jspui/handle/tede/871Leptospirosis is considered a global health problem in human and veterinary medicine. It is a zoonotic disease caused by bacteria of the genus Leptospira, prevalent on every continent. The development of an effective macroscopic serum agglutination test against leptospirosis is justified, since the available ones are expensive, cumbersome and restricted to specialized laboratories. In Brazil, leptospirosis in pigs has been a major cause of reproductive failure in several states, mainly in the south and southeast regions of the country. Among the animals used in production of rural ecosystems, pigs have the most frequent clinical manifestations in the reproductive sphere with abortions, usually in the final third of pregnancy. Infertility, sterility or birth of debilitated or dead pigs which dies in the first days of life are signs of the presence of the bacteria in the breeding matrix. In the present study, six suspensions formulations of antigenic serovars pomona, icterohaemorrhagiae and copenhageni were used in single suspension form or in two serovars combinations. These were tested against pig serum with no clinical suspicion of leptospirosis compared with the microscopic agglutination test that is accepted as the gold standard by the World Health Organization (WHO). Among the six proposed suspensions the combination of serovars pomona + icterohaemorrhagiae showed the best result. The results demonstrates a percentage of 89% sensitivity and specificity of 89% for the suspension with the serovars combination of pomona + icterohaemorrhagiae and 87% for positive predictive value and 90% negative predictive value for the same combination, demonstrated by the statistics methods such as Chi-square, Kappa and Linear Logistic Model. Given the importance of leptospirosis in pigs and economic impact that it causes worldwide, in addition to the lack of current information, this study aimed to develop a screening rapid test for the diagnosis of pigs kept in variable dimension farms from rural regions of the Rio de Janeiro state.A leptospirose ? considerada um problema global de sa?de na ?rea humana e veterin?ria. ? uma zoonose causada por bact?rias do g?nero Leptospira, prevalente em todos os continentes. O desenvolvimento de um teste de soro aglutina??o macrosc?pica eficaz contra a leptospirose se justifica, j? que os existentes s?o caros, trabalhosos e restritos a laborat?rios especializados. No Brasil, a leptospirose em su?nos tem sido uma das principais causas de falhas reprodutivas em v?rios estados, principalmente nas regi?es sul e sudeste do pa?s. Dentre os animais de produ??o explorados em ecossistemas rurais os su?nos apresentam as manifesta??es cl?nicas mais freq?entes na esfera reprodutiva com abortamentos, usualmente no ter?o final da gesta??o. A infertilidade, a esterilidade o nascimento de leit?es debilitados que morrem nos primeiros dias de vida s?o sinais da presen?a da bact?ria nas matrizes reprodutoras. No presente estudo, utilizou-se seis formula??es de suspens?es antig?nicas dos sorovares pomona, icterohaemorrhagiae e copenhageni em forma de suspens?o ?nica ou em combina??es de dois sorovares. Foram testadas frente a soros de su?nos sem suspeita cl?nica de leptospirose comparando com a soroaglutina??o microsc?pica que ? o teste aceito como padr?o ouro pela Organiza??o Mundial de Sa?de (OMS). Dentre as seis suspens?es propostas a combina??o dos sorovares pomona + icterohaemorrhagiae apresentou o melhor resultado. Os resultados obtidos demonstraram um percentual de 89% de sensibilidade e 89% de especificidade para a suspens?o com a combina??o dos sorovares pomona + icterohaemorrhagiae e de 87% de valor preditivo positivo e 90% de valor preditivo negativo para a mesma combina??o, comprovado pelos m?todos estat?sticos Qui-quadrado, Kappa e Modelo Linear Log?stico. Dada ? import?ncia da leptospirose em su?nos e o impacto econ?mico que causa mundialmente, al?m da car?ncia de informa??es atuais, este trabalho teve por objetivo desenvolver um teste r?pido de triagem para diagn?stico em su?nos mantidos em cria??es rurais de diferentes regi?es e tamanhos no Estado do Rio de Janeiro.Made available in DSpace on 2016-04-28T20:17:28Z (GMT). No. of bitstreams: 1 2008 - Gerson Silva de Lima.pdf: 864257 bytes, checksum: ca49b40df55461f84d34f8a4d94fd512 (MD5) Previous issue date: 2008-10-03application/pdfhttps://tede.ufrrj.br/retrieve/60242/2008%20-%20Gerson%20Silva%20de%20Lima.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Microbiologia Veterin?riaUFRRJBrasilInstituto de Veterin?riaLeptospirosediagn?sticoMacroaglutina??osu?nos.LeptospirosisdiagnosisMacroagglutinationpigMicrobiologiaPadroniza??o de um teste de soroaglutina??o macrosc?pica para diagn?stico da leptospirose em su?nosStandardization of a Macroscopic Seroagglutination test for the diagnostic of leptospirosis in pigsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2008 - Gerson Silva de Lima.pdf.jpg2008 - Gerson Silva de Lima.pdf.jpgimage/jpeg3801http://localhost:8080/tede/bitstream/tede/871/3/2008+-+Gerson+Silva+de+Lima.pdf.jpg015e7f42510d1ea34889cad4455e1f35MD53TEXT2008 - Gerson Silva de Lima.pdf.txt2008 - Gerson Silva de Lima.pdf.txttext/plain94190http://localhost:8080/tede/bitstream/tede/871/2/2008+-+Gerson+Silva+de+Lima.pdf.txtf49099a6ab636ac714686743df54fb3bMD52ORIGINAL2008 - Gerson Silva de Lima.pdf2008 - Gerson Silva de Lima.pdfapplication/pdf845619http://localhost:8080/tede/bitstream/tede/871/1/2008+-+Gerson+Silva+de+Lima.pdf7ff88373386b59b7cd5b9da1bd3e4488MD51tede/8712022-06-20 10:00:37.105oai:localhost:tede/871Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-06-20T13:00:37Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
dc.title.por.fl_str_mv |
Padroniza??o de um teste de soroaglutina??o macrosc?pica para diagn?stico da leptospirose em su?nos |
dc.title.alternative.eng.fl_str_mv |
Standardization of a Macroscopic Seroagglutination test for the diagnostic of leptospirosis in pigs |
title |
Padroniza??o de um teste de soroaglutina??o macrosc?pica para diagn?stico da leptospirose em su?nos |
spellingShingle |
Padroniza??o de um teste de soroaglutina??o macrosc?pica para diagn?stico da leptospirose em su?nos Lima, Gerson Silva de Leptospirose diagn?stico Macroaglutina??o su?nos. Leptospirosis diagnosis Macroagglutination pig Microbiologia |
title_short |
Padroniza??o de um teste de soroaglutina??o macrosc?pica para diagn?stico da leptospirose em su?nos |
title_full |
Padroniza??o de um teste de soroaglutina??o macrosc?pica para diagn?stico da leptospirose em su?nos |
title_fullStr |
Padroniza??o de um teste de soroaglutina??o macrosc?pica para diagn?stico da leptospirose em su?nos |
title_full_unstemmed |
Padroniza??o de um teste de soroaglutina??o macrosc?pica para diagn?stico da leptospirose em su?nos |
title_sort |
Padroniza??o de um teste de soroaglutina??o macrosc?pica para diagn?stico da leptospirose em su?nos |
author |
Lima, Gerson Silva de |
author_facet |
Lima, Gerson Silva de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Flausino, Walter |
dc.contributor.advisor1ID.fl_str_mv |
368.511.757-20 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788774D0 |
dc.contributor.authorID.fl_str_mv |
737.338.307-68 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7334395107841698 |
dc.contributor.author.fl_str_mv |
Lima, Gerson Silva de |
contributor_str_mv |
Flausino, Walter |
dc.subject.por.fl_str_mv |
Leptospirose diagn?stico Macroaglutina??o su?nos. |
topic |
Leptospirose diagn?stico Macroaglutina??o su?nos. Leptospirosis diagnosis Macroagglutination pig Microbiologia |
dc.subject.eng.fl_str_mv |
Leptospirosis diagnosis Macroagglutination pig |
dc.subject.cnpq.fl_str_mv |
Microbiologia |
description |
Leptospirosis is considered a global health problem in human and veterinary medicine. It is a zoonotic disease caused by bacteria of the genus Leptospira, prevalent on every continent. The development of an effective macroscopic serum agglutination test against leptospirosis is justified, since the available ones are expensive, cumbersome and restricted to specialized laboratories. In Brazil, leptospirosis in pigs has been a major cause of reproductive failure in several states, mainly in the south and southeast regions of the country. Among the animals used in production of rural ecosystems, pigs have the most frequent clinical manifestations in the reproductive sphere with abortions, usually in the final third of pregnancy. Infertility, sterility or birth of debilitated or dead pigs which dies in the first days of life are signs of the presence of the bacteria in the breeding matrix. In the present study, six suspensions formulations of antigenic serovars pomona, icterohaemorrhagiae and copenhageni were used in single suspension form or in two serovars combinations. These were tested against pig serum with no clinical suspicion of leptospirosis compared with the microscopic agglutination test that is accepted as the gold standard by the World Health Organization (WHO). Among the six proposed suspensions the combination of serovars pomona + icterohaemorrhagiae showed the best result. The results demonstrates a percentage of 89% sensitivity and specificity of 89% for the suspension with the serovars combination of pomona + icterohaemorrhagiae and 87% for positive predictive value and 90% negative predictive value for the same combination, demonstrated by the statistics methods such as Chi-square, Kappa and Linear Logistic Model. Given the importance of leptospirosis in pigs and economic impact that it causes worldwide, in addition to the lack of current information, this study aimed to develop a screening rapid test for the diagnosis of pigs kept in variable dimension farms from rural regions of the Rio de Janeiro state. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-10-03 |
dc.date.available.fl_str_mv |
2009-02-03 |
dc.date.accessioned.fl_str_mv |
2016-04-28T20:17:28Z |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
LIMA, Gerson Silva de. Padroniza??o de um teste de soroaglutina??o macrosc?pica para diagn?stico da Leptospirose em su?nos. 2008. 97 f. Disserta??o (Mestrado em Microbiologia Veterin?ria) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2008. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/tede/871 |
identifier_str_mv |
LIMA, Gerson Silva de. Padroniza??o de um teste de soroaglutina??o macrosc?pica para diagn?stico da Leptospirose em su?nos. 2008. 97 f. Disserta??o (Mestrado em Microbiologia Veterin?ria) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2008. |
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https://tede.ufrrj.br/jspui/handle/tede/871 |
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por |
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Universidade Federal Rural do Rio de Janeiro |
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UFRRJ |
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Brasil |
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Instituto de Veterin?ria |
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Universidade Federal Rural do Rio de Janeiro |
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