Adição de ácido fítico na dieta de jundiá (Rhamdia quelen)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/16109 |
Resumo: | This work presents a study on the action of phytic acid in performance, metabolism and antioxidant activity of jundiá (Rhamdia quelen). Thirteen juveniles of jundúia averaging 7 ± 2.30g were randomly distributed in 20 polyethylene tanks of 70L (15 fish per tank) in a water recirculation system, and were fed for 50 days. Five diets were formulated, four with different levels of phytic acid (0.5% PA, 1.0% PA, 1.5% PA or 2.0% PA) and a control diet, that is, without the addition of phytic acid (0% PA). Four replicates were used for each set. The animals were fed three times daily to an apparent satiation. At the end of the experimental period, growth variables (final weight gain, specific growth rate, protein ratio and redundant food production) and carcass parameters (carcass yield, intestinal quotient, digestive index) were evaluated. hepatic-somatic and visceral fat). In addition, metabolic parameters (albumin, amino acids, total proteins, triglycerides, cholesterol, glucose) and hepatic parameters (protein, glycogen, glucose and ammonia) were selected. Also tested as activities of the enzymes trypsin, chymotrypsin, intestinal lipase and acid protease. No whole fish was analyzed at a centesimal (dry matter, mineral matter, crude protein and fat). The action of phytic acid as a pro-oxidant was tested with carbonyl and glutathione reductase proteins, in addition to the activity of the enzymes superoxide dismutase, catalase, glutathione peroxidase and glutathione S-transferase. Genes that received 0.5% AF had the visceral fat index and hepatic glycogen decreased. The 2.0% PA diet promotes the activity of the enzyme protease acid. The 1.5% of PA increased protein levels of any level of phytic acid in the diet, decreased fat concentration in whole fish composition. Fish that were fed 0.5% and 1.0% AF had lower carbonyl protein formation in both the liver and gills. Animals receiving 0.5% phytic acid in the diet had higher levels of GSH in the liver and gills, as well as greater activities of the GST and GPX enzymes (in the hepatic tissue). SOD and CAT activities in the liver were higher in fish receiving 0.5% and 1.0% PA. With this study it was possible to conclude that the presence of low concentrations of phytic acid in the diet of jundiá contributes to the improvement in fish quality and as an oxidative moderator. |
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Adição de ácido fítico na dieta de jundiá (Rhamdia quelen)Addition of phytic acid in silver catfish (Rhamdia quelen) dietPeixeNutrição animalAditivoModerador oxidativoMioinositol 6-fosfatoFishAnimal nutritionAdditiveOxidative moderatorMyoinositol 6 phosphateCNPQ::CIENCIAS AGRARIAS::ZOOTECNIAThis work presents a study on the action of phytic acid in performance, metabolism and antioxidant activity of jundiá (Rhamdia quelen). Thirteen juveniles of jundúia averaging 7 ± 2.30g were randomly distributed in 20 polyethylene tanks of 70L (15 fish per tank) in a water recirculation system, and were fed for 50 days. Five diets were formulated, four with different levels of phytic acid (0.5% PA, 1.0% PA, 1.5% PA or 2.0% PA) and a control diet, that is, without the addition of phytic acid (0% PA). Four replicates were used for each set. The animals were fed three times daily to an apparent satiation. At the end of the experimental period, growth variables (final weight gain, specific growth rate, protein ratio and redundant food production) and carcass parameters (carcass yield, intestinal quotient, digestive index) were evaluated. hepatic-somatic and visceral fat). In addition, metabolic parameters (albumin, amino acids, total proteins, triglycerides, cholesterol, glucose) and hepatic parameters (protein, glycogen, glucose and ammonia) were selected. Also tested as activities of the enzymes trypsin, chymotrypsin, intestinal lipase and acid protease. No whole fish was analyzed at a centesimal (dry matter, mineral matter, crude protein and fat). The action of phytic acid as a pro-oxidant was tested with carbonyl and glutathione reductase proteins, in addition to the activity of the enzymes superoxide dismutase, catalase, glutathione peroxidase and glutathione S-transferase. Genes that received 0.5% AF had the visceral fat index and hepatic glycogen decreased. The 2.0% PA diet promotes the activity of the enzyme protease acid. The 1.5% of PA increased protein levels of any level of phytic acid in the diet, decreased fat concentration in whole fish composition. Fish that were fed 0.5% and 1.0% AF had lower carbonyl protein formation in both the liver and gills. Animals receiving 0.5% phytic acid in the diet had higher levels of GSH in the liver and gills, as well as greater activities of the GST and GPX enzymes (in the hepatic tissue). SOD and CAT activities in the liver were higher in fish receiving 0.5% and 1.0% PA. With this study it was possible to conclude that the presence of low concentrations of phytic acid in the diet of jundiá contributes to the improvement in fish quality and as an oxidative moderator.Este trabalho apresenta um estudo sobre a ação do ácido fítico no desempenho produtivo, metabolismo e atividade antioxidante de jundiá (Rhamdia quelen). Trezentos juvenis de jundiá com média de 7±2,30g foram distribuídos aleatoriamente em 20 tanques de polietileno de 70L (15 peixes por tanque) em sistema de recirculação de água, e foram alimentados durante 50 dias. Foram formuladas cinco dietas, sendo quatro com diferentes níveis de ácido fítico (0,5%AF, 1,0%AF, 1,5%AF ou 2,0%AF) e uma dieta controle, ou seja, sem adição de ácido fítico (0%AF). Para cada tratamento foram utilizadas quatro repetições. Os animais foram alimentados três vezes ao dia até a saciedade aparente. Ao final do período experimental foram avaliadas: variáveis de crescimento (ganho de peso final, ganho de peso relativo, taxa de crescimento específico, taxa de eficiência proteica e conversão alimentar aparente) e parâmetros de carcaça (rendimento de caracaça, quociente intestinal, índice digestivo-somático, hepato-somático e de gordura visceral). Além disso, foram determinados parâmetros metabolicos (albumina, aminoácidos, proteínas totais, triglicerídeos, colesterol, glicose) e hepáticos (proteína, glicogênio, glicose e amônia). Também foram aferidas as atividades das enzimas tripsina, quimiotripsina, lipase intestinal e protease ácida. No peixe inteiro foi analisada a composição centesimal (matéria seca, matéria mineral, proteína bruta e gordura). A ação do ácido fítico como pró-oxidante foi testada com as análises de proteína carbonil e glutationa redutase, além da atividade das enzimas superóxido dismutase, catalase, glutationa peroxidase e glutationa S-transferase. Os peixes que receberam 0,5%AF tiveram o índice de gordura visceral e glicogênio hepático diminuídos. A dieta com de 2,0% de AF resultou em menor atividade da enzima protease ácida. A adição de 1,5%AF aumentou os teores de proteína, e qualquer nível de ácido fítico na dieta diminuiu a concentração de gordura na composição do peixe inteiro. Os peixes que foram alimentados com 0,5% e 1,0% AF tiveram menor formação de proteína carbonil no fígado e nas brânquias. Os animais que receberam 0.5% de ácido fítico na dieta tiveram maiores níveis de GSH no fígado e nas brânquias, assim como maiores atividades das enzimas GST e GPX (no tecido hepático). As atividades de SOD e CAT no fígado foram maiores nos peixes que receberam 0,5% e 1,0% AF. Com este estudo foi possível concluir que a presença de baixas concentrações de ácido fítico na dieta do jundiá contribui para melhor a qualidade do pescado e como moderador oxidativo.Universidade Federal de Santa MariaBrasilZootecniaUFSMPrograma de Pós-Graduação em ZootecniaCentro de Ciências RuraisSilva, Leila Picolli dahttp://lattes.cnpq.br/9378190351379861Loro, Vania Luciahttp://lattes.cnpq.br/6392817606416780Veiverberg, Cátia Alinehttp://lattes.cnpq.br/9474062270578041Costa, Letícia Lopes da2019-04-09T13:01:58Z2019-04-09T13:01:58Z2018-08-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/16109porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2019-04-10T06:01:54Zoai:repositorio.ufsm.br:1/16109Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2019-04-10T06:01:54Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Adição de ácido fítico na dieta de jundiá (Rhamdia quelen) Addition of phytic acid in silver catfish (Rhamdia quelen) diet |
title |
Adição de ácido fítico na dieta de jundiá (Rhamdia quelen) |
spellingShingle |
Adição de ácido fítico na dieta de jundiá (Rhamdia quelen) Costa, Letícia Lopes da Peixe Nutrição animal Aditivo Moderador oxidativo Mioinositol 6-fosfato Fish Animal nutrition Additive Oxidative moderator Myoinositol 6 phosphate CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
title_short |
Adição de ácido fítico na dieta de jundiá (Rhamdia quelen) |
title_full |
Adição de ácido fítico na dieta de jundiá (Rhamdia quelen) |
title_fullStr |
Adição de ácido fítico na dieta de jundiá (Rhamdia quelen) |
title_full_unstemmed |
Adição de ácido fítico na dieta de jundiá (Rhamdia quelen) |
title_sort |
Adição de ácido fítico na dieta de jundiá (Rhamdia quelen) |
author |
Costa, Letícia Lopes da |
author_facet |
Costa, Letícia Lopes da |
author_role |
author |
dc.contributor.none.fl_str_mv |
Silva, Leila Picolli da http://lattes.cnpq.br/9378190351379861 Loro, Vania Lucia http://lattes.cnpq.br/6392817606416780 Veiverberg, Cátia Aline http://lattes.cnpq.br/9474062270578041 |
dc.contributor.author.fl_str_mv |
Costa, Letícia Lopes da |
dc.subject.por.fl_str_mv |
Peixe Nutrição animal Aditivo Moderador oxidativo Mioinositol 6-fosfato Fish Animal nutrition Additive Oxidative moderator Myoinositol 6 phosphate CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
topic |
Peixe Nutrição animal Aditivo Moderador oxidativo Mioinositol 6-fosfato Fish Animal nutrition Additive Oxidative moderator Myoinositol 6 phosphate CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
description |
This work presents a study on the action of phytic acid in performance, metabolism and antioxidant activity of jundiá (Rhamdia quelen). Thirteen juveniles of jundúia averaging 7 ± 2.30g were randomly distributed in 20 polyethylene tanks of 70L (15 fish per tank) in a water recirculation system, and were fed for 50 days. Five diets were formulated, four with different levels of phytic acid (0.5% PA, 1.0% PA, 1.5% PA or 2.0% PA) and a control diet, that is, without the addition of phytic acid (0% PA). Four replicates were used for each set. The animals were fed three times daily to an apparent satiation. At the end of the experimental period, growth variables (final weight gain, specific growth rate, protein ratio and redundant food production) and carcass parameters (carcass yield, intestinal quotient, digestive index) were evaluated. hepatic-somatic and visceral fat). In addition, metabolic parameters (albumin, amino acids, total proteins, triglycerides, cholesterol, glucose) and hepatic parameters (protein, glycogen, glucose and ammonia) were selected. Also tested as activities of the enzymes trypsin, chymotrypsin, intestinal lipase and acid protease. No whole fish was analyzed at a centesimal (dry matter, mineral matter, crude protein and fat). The action of phytic acid as a pro-oxidant was tested with carbonyl and glutathione reductase proteins, in addition to the activity of the enzymes superoxide dismutase, catalase, glutathione peroxidase and glutathione S-transferase. Genes that received 0.5% AF had the visceral fat index and hepatic glycogen decreased. The 2.0% PA diet promotes the activity of the enzyme protease acid. The 1.5% of PA increased protein levels of any level of phytic acid in the diet, decreased fat concentration in whole fish composition. Fish that were fed 0.5% and 1.0% AF had lower carbonyl protein formation in both the liver and gills. Animals receiving 0.5% phytic acid in the diet had higher levels of GSH in the liver and gills, as well as greater activities of the GST and GPX enzymes (in the hepatic tissue). SOD and CAT activities in the liver were higher in fish receiving 0.5% and 1.0% PA. With this study it was possible to conclude that the presence of low concentrations of phytic acid in the diet of jundiá contributes to the improvement in fish quality and as an oxidative moderator. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-08-10 2019-04-09T13:01:58Z 2019-04-09T13:01:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/16109 |
url |
http://repositorio.ufsm.br/handle/1/16109 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Zootecnia UFSM Programa de Pós-Graduação em Zootecnia Centro de Ciências Rurais |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Zootecnia UFSM Programa de Pós-Graduação em Zootecnia Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922084097884160 |