Caracterização molecular de Rhodococcus equi de potros pela PCR multiplex dos genes da família vap
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/10016 |
Resumo: | This study evaluated molecular characteristic of Rhodococcus equi isolates obtained from horses and standardized by PCR multiplex assay, which amplifies the vap gene family (vapA, B, C, D, E, F, G e H). One hundred eighty Rhodococcus equi isolates from different sources: healthy horse s feces (112), soil (12), stalls (23) and clinical isolates (33) of horsebreeding farms, were studied. The technique was standardized and confirmed by sequencing of amplified vap gene family controls. Thirty-two (17.8%) R. equi isolates evaluated were positive for vapA gene and carried at least three another vap genes associated. All 147 isolates from equine feces, stalls and soil from horse-breeding farms did not demonstrate any virulence-associated proteins genes. Thirty-two (97.0%) out of 33 clinical equines isolates were positive to multiplex PCR assay for vap gene family and demonstrated six molecular profile, 100% with vapA, vapD and vapG genes, 86.6% vapF, 76,6% vapH, 43.3% vapC, 36.6% vapE and none vapB. The most frequent molecular profile was vap A, D, F, G and H present in 37.5% of strains. Morever, there was no molecular epidemiological pattern for R. equi isolates from each horse-breeding farm studied. Thus this technique allows the identification of eight vap genes family (vapA, B, C, D, E, F, G e H), it is a practical an efficient method of conducting clinical and epidemiological studies on R. equi isolates. |
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2017-06-022017-06-022008-02-19MONEGO, Fernanda. Molecular characterization of Rhodococcus equi from foals by multiplex PCR for vap genes. 2008. 49 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2008.http://repositorio.ufsm.br/handle/1/10016This study evaluated molecular characteristic of Rhodococcus equi isolates obtained from horses and standardized by PCR multiplex assay, which amplifies the vap gene family (vapA, B, C, D, E, F, G e H). One hundred eighty Rhodococcus equi isolates from different sources: healthy horse s feces (112), soil (12), stalls (23) and clinical isolates (33) of horsebreeding farms, were studied. The technique was standardized and confirmed by sequencing of amplified vap gene family controls. Thirty-two (17.8%) R. equi isolates evaluated were positive for vapA gene and carried at least three another vap genes associated. All 147 isolates from equine feces, stalls and soil from horse-breeding farms did not demonstrate any virulence-associated proteins genes. Thirty-two (97.0%) out of 33 clinical equines isolates were positive to multiplex PCR assay for vap gene family and demonstrated six molecular profile, 100% with vapA, vapD and vapG genes, 86.6% vapF, 76,6% vapH, 43.3% vapC, 36.6% vapE and none vapB. The most frequent molecular profile was vap A, D, F, G and H present in 37.5% of strains. Morever, there was no molecular epidemiological pattern for R. equi isolates from each horse-breeding farm studied. Thus this technique allows the identification of eight vap genes family (vapA, B, C, D, E, F, G e H), it is a practical an efficient method of conducting clinical and epidemiological studies on R. equi isolates.O presente estudo tem por objetivo a caracterização molecular de isolados de Rhodococcus equi de eqüinos pela padronização de uma técnica de PCR multiplex para detecção dos genes da família vap (vapA, B, C, D, E, F, G e H). Foram analisadas 180 amostras de Rhodococcus equi de diferentes origens: fezes (112), solo (12) instalações (23) e isolados clínicos (33). A técnica foi padronizada e confirmada pelo sequenciamento da cepa padrão de R. equi (ATCC 33701), e de uma amostra de paciente humano contendo o gene vapB. Trinta e dois (17,8%) foram positivos para vapA e carregavam no mínimo 4 genes vap associados. Os 147 isolados oriundos de fezes, instalações e sola não apresentavam genes vap. Trinta e dois (97.0%) dos isolados clinicos foram positivos na PCR multiplex e demonstraram seis padrões moleculares, 100% com vapA, vapD and vapG, 86.6% vapF, 76,6% vapH, 43.3% vapC, 36.6% vapE e nenhum com vapB. O perfil molecular mais freqüente foi vap A, D, F, G eH presente em 37.5% das cepas. foram obtidos, sendo que os genes vapA, vapD e vapG estavam presentes em todas as amostras. Não foi obtido nenhum padrão molecular para cada propriedade estudada. Esta nova técnica constitui-se um método prático e eficaz para condução de estudos clínicos e epidemiológicos, bem como, por relevar os aspectos moleculares da infecção.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Medicina VeterináriaUFSMBRMedicina VeterináriaRhodococcus equiVapPCR multiplexPotrosFezesRhodococcus equiVap genesPCR multiplexFoalsFecesCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIACaracterização molecular de Rhodococcus equi de potros pela PCR multiplex dos genes da família vapMolecular characterization of Rhodococcus equi from foals by multiplex PCR for vap genesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisVargas, Agueda Palmira Castagna dehttp://lattes.cnpq.br/1383126157031968Ribeiro, Marcio Garciahttp://lattes.cnpq.br/2209124317273797Loreto, Elgion Lucio da Silvahttp://lattes.cnpq.br/6493669115018157http://lattes.cnpq.br/7197850876016537Monego, Fernanda5005000000074005003003005007561081c-bfab-4ea7-ace8-2bf6488cb0b592938a59-7941-4d6e-8af6-b54668a93934e67befc3-dd28-4638-890f-0a49e4182ccbab41f5b8-3dea-4caa-86ab-c37a1044a379info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALFERNANDAMONEGO.pdfapplication/pdf239375http://repositorio.ufsm.br/bitstream/1/10016/1/FERNANDAMONEGO.pdffdc6c63a44bae74c615b641ba68b2b26MD51TEXTFERNANDAMONEGO.pdf.txtFERNANDAMONEGO.pdf.txtExtracted texttext/plain76236http://repositorio.ufsm.br/bitstream/1/10016/2/FERNANDAMONEGO.pdf.txt0eb3a1023c9d7be25d5ee75b0f937416MD52THUMBNAILFERNANDAMONEGO.pdf.jpgFERNANDAMONEGO.pdf.jpgIM Thumbnailimage/jpeg4943http://repositorio.ufsm.br/bitstream/1/10016/3/FERNANDAMONEGO.pdf.jpg7296ad134a825d28ff91a6016047784dMD531/100162022-03-15 14:29:48.21oai:repositorio.ufsm.br:1/10016Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-03-15T17:29:48Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Caracterização molecular de Rhodococcus equi de potros pela PCR multiplex dos genes da família vap |
dc.title.alternative.eng.fl_str_mv |
Molecular characterization of Rhodococcus equi from foals by multiplex PCR for vap genes |
title |
Caracterização molecular de Rhodococcus equi de potros pela PCR multiplex dos genes da família vap |
spellingShingle |
Caracterização molecular de Rhodococcus equi de potros pela PCR multiplex dos genes da família vap Monego, Fernanda Rhodococcus equi Vap PCR multiplex Potros Fezes Rhodococcus equi Vap genes PCR multiplex Foals Feces CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Caracterização molecular de Rhodococcus equi de potros pela PCR multiplex dos genes da família vap |
title_full |
Caracterização molecular de Rhodococcus equi de potros pela PCR multiplex dos genes da família vap |
title_fullStr |
Caracterização molecular de Rhodococcus equi de potros pela PCR multiplex dos genes da família vap |
title_full_unstemmed |
Caracterização molecular de Rhodococcus equi de potros pela PCR multiplex dos genes da família vap |
title_sort |
Caracterização molecular de Rhodococcus equi de potros pela PCR multiplex dos genes da família vap |
author |
Monego, Fernanda |
author_facet |
Monego, Fernanda |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Vargas, Agueda Palmira Castagna de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1383126157031968 |
dc.contributor.referee1.fl_str_mv |
Ribeiro, Marcio Garcia |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/2209124317273797 |
dc.contributor.referee2.fl_str_mv |
Loreto, Elgion Lucio da Silva |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/6493669115018157 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7197850876016537 |
dc.contributor.author.fl_str_mv |
Monego, Fernanda |
contributor_str_mv |
Vargas, Agueda Palmira Castagna de Ribeiro, Marcio Garcia Loreto, Elgion Lucio da Silva |
dc.subject.por.fl_str_mv |
Rhodococcus equi Vap PCR multiplex Potros Fezes |
topic |
Rhodococcus equi Vap PCR multiplex Potros Fezes Rhodococcus equi Vap genes PCR multiplex Foals Feces CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Rhodococcus equi Vap genes PCR multiplex Foals Feces |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
This study evaluated molecular characteristic of Rhodococcus equi isolates obtained from horses and standardized by PCR multiplex assay, which amplifies the vap gene family (vapA, B, C, D, E, F, G e H). One hundred eighty Rhodococcus equi isolates from different sources: healthy horse s feces (112), soil (12), stalls (23) and clinical isolates (33) of horsebreeding farms, were studied. The technique was standardized and confirmed by sequencing of amplified vap gene family controls. Thirty-two (17.8%) R. equi isolates evaluated were positive for vapA gene and carried at least three another vap genes associated. All 147 isolates from equine feces, stalls and soil from horse-breeding farms did not demonstrate any virulence-associated proteins genes. Thirty-two (97.0%) out of 33 clinical equines isolates were positive to multiplex PCR assay for vap gene family and demonstrated six molecular profile, 100% with vapA, vapD and vapG genes, 86.6% vapF, 76,6% vapH, 43.3% vapC, 36.6% vapE and none vapB. The most frequent molecular profile was vap A, D, F, G and H present in 37.5% of strains. Morever, there was no molecular epidemiological pattern for R. equi isolates from each horse-breeding farm studied. Thus this technique allows the identification of eight vap genes family (vapA, B, C, D, E, F, G e H), it is a practical an efficient method of conducting clinical and epidemiological studies on R. equi isolates. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-02-19 |
dc.date.accessioned.fl_str_mv |
2017-06-02 |
dc.date.available.fl_str_mv |
2017-06-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MONEGO, Fernanda. Molecular characterization of Rhodococcus equi from foals by multiplex PCR for vap genes. 2008. 49 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2008. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/10016 |
identifier_str_mv |
MONEGO, Fernanda. Molecular characterization of Rhodococcus equi from foals by multiplex PCR for vap genes. 2008. 49 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2008. |
url |
http://repositorio.ufsm.br/handle/1/10016 |
dc.language.iso.fl_str_mv |
por |
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por |
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500500000007 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
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Programa de Pós-Graduação em Medicina Veterinária |
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UFSM |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Medicina Veterinária |
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Universidade Federal de Santa Maria |
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