Papel da terapia fotodinâmica antimicrobiana adjuvante ao tratamento periodontal sob parâmetros de estresse oxidativo plasmático e comportamento vascular

Detalhes bibliográficos
Autor(a) principal: Barin, Luisa Machado
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
Texto Completo: http://repositorio.ufsm.br/handle/1/6159
Resumo: The aim of this study was to evaluate the effects of methylene blue (MB) photosensitizer (PS) solubilized in ethanol in antimicrobial photodynamic therapy (aPDT) as an adjuvant periodontal treatment, at oxidative stress (OS) parameters and vascular behavior in rat model. Besides, investigate greater involvement of angiogenesis at the destruction or healing stages of periodontal disease (PD). One hundred and twenty male adult Wistar rats were divided randomly into five groups. The control groups were negative control (NC) (no PD, n=15) and positive control (PC) (with PD, without any treatment, n=15). The other groups had PD and underwent different types of treatment, such as scaling and root planing (SRP) and irrigation with 1mL of saline solution (SRP, n=30); SRP and aPDT with MB solubilized in water (aPDT I, n=30); SRP and aPDT with MB containing ethanol (aPDT II, n=30). The PD was induced via the installation of a ligature around the mandibular right first molar. After 7 days, the ligature was removed and animals received treatment. At 7, 15 and 30 days, rats were euthanized and the gingival tissue surrounding the induction area was removed for histomorphometric analysis of the number and diameter of blood vessels by staining with hematoxylin and eosin (H&E). The collected blood was centrifuged and the plasma was used to determine lipid peroxidation by quantifying thiobarbituric acid reactive substances (TBARS), vitamin C (VIT C) and glutathione reduced (GSH) levels. The oxidative status showed higher TBARS levels in PC group in 7, 15 and 30 days, and indicated a protective influence of aPDT II on plasma observed from lower lipid peroxidation. GSH levels were consumed in PC, aPDT I and aPDT II groups throughout the experiment. Furthermore, aPDT II also increased antioxidant defenses in plasma: i) higher levels of GSH, and ii) increased levels of VIT C. Interestingly, the VIT C plasmatic levels were restored in the aPDT II group in the 30th experimental day. Histomorphometric findings in 7 days showed that treated groups (SRP, aPDT I and aPDT II) showed higher number of blood vessels, and the aPDT II group showed the highest values among them. From these results, aPDT modifies PD course, reducing oxidative systemic damage and stimulating the antioxidant defense system, thus protecting the areas closely affected by PD in rats. Moreover, was observed a relationship between increased expression of angiogenesis and repair stage of the PD. In summary, we suggest that the aPDT with MB solubilized in ethanol provides better therapeutic responses in periodontal treatment.
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spelling Papel da terapia fotodinâmica antimicrobiana adjuvante ao tratamento periodontal sob parâmetros de estresse oxidativo plasmático e comportamento vascularRole of the adjunctive antimicrobial photodynamic therapy to periodontal treatment at plasmatic oxidative stress parameters and vascular behaviorAzul de metilenoEstresse oxidativoEtanolNeovascularizaçãoTerapia fotodinâmica antimicrobianaAntimicrobial photodynamic therapyEthanolMethylene blueNeovascularizationOxidative stressCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAThe aim of this study was to evaluate the effects of methylene blue (MB) photosensitizer (PS) solubilized in ethanol in antimicrobial photodynamic therapy (aPDT) as an adjuvant periodontal treatment, at oxidative stress (OS) parameters and vascular behavior in rat model. Besides, investigate greater involvement of angiogenesis at the destruction or healing stages of periodontal disease (PD). One hundred and twenty male adult Wistar rats were divided randomly into five groups. The control groups were negative control (NC) (no PD, n=15) and positive control (PC) (with PD, without any treatment, n=15). The other groups had PD and underwent different types of treatment, such as scaling and root planing (SRP) and irrigation with 1mL of saline solution (SRP, n=30); SRP and aPDT with MB solubilized in water (aPDT I, n=30); SRP and aPDT with MB containing ethanol (aPDT II, n=30). The PD was induced via the installation of a ligature around the mandibular right first molar. After 7 days, the ligature was removed and animals received treatment. At 7, 15 and 30 days, rats were euthanized and the gingival tissue surrounding the induction area was removed for histomorphometric analysis of the number and diameter of blood vessels by staining with hematoxylin and eosin (H&E). The collected blood was centrifuged and the plasma was used to determine lipid peroxidation by quantifying thiobarbituric acid reactive substances (TBARS), vitamin C (VIT C) and glutathione reduced (GSH) levels. The oxidative status showed higher TBARS levels in PC group in 7, 15 and 30 days, and indicated a protective influence of aPDT II on plasma observed from lower lipid peroxidation. GSH levels were consumed in PC, aPDT I and aPDT II groups throughout the experiment. Furthermore, aPDT II also increased antioxidant defenses in plasma: i) higher levels of GSH, and ii) increased levels of VIT C. Interestingly, the VIT C plasmatic levels were restored in the aPDT II group in the 30th experimental day. Histomorphometric findings in 7 days showed that treated groups (SRP, aPDT I and aPDT II) showed higher number of blood vessels, and the aPDT II group showed the highest values among them. From these results, aPDT modifies PD course, reducing oxidative systemic damage and stimulating the antioxidant defense system, thus protecting the areas closely affected by PD in rats. Moreover, was observed a relationship between increased expression of angiogenesis and repair stage of the PD. In summary, we suggest that the aPDT with MB solubilized in ethanol provides better therapeutic responses in periodontal treatment.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorO objetivo deste estudo foi avaliar os efeitos do fotossensibilizador (Fs) azul de metileno (AM) solubilizado em etanol na terapia fotodinâmica antimicrobiana (TFDa) como um tratamento periodontal adjuvante, sob parâmetros de estresse oxidativo (EO) e de comportamento vascular em modelo de rato. Bem como, investigar o maior envolvimento da angiogênese no estágio de avanço ou reparo da doença periodontal (DP). Cento e vinte ratos Wistar adultos machos foram randomizados e divididos em cinco grupos. Os grupos controles eram controle negativo (CN) (sem DP, n = 15) e controle positivo (CP) (com DP, sem qualquer tratamento, n = 15). Os demais grupos apresentavam DP e foram submetidos a diferentes tipos de tratamentos, como raspagem e alisamento radicular (RAR) e irrigação com 1 mL de solução salina (RAR, n = 30); RAR e TFDa com AM solubilizado em água (TFDa I, n = 30); RAR e TFDa com AM contendo etanol (TFDa II, n = 30). A DP foi induzida através da colocação de uma ligadura ao redor do primeiro molar inferior direito. Após 7 dias, a ligadura foi removida e os animais receberam tratamento. Aos 7, 15 e 30 dias, os ratos foram eutanasiados e o tecido gengival circundante à área de indução foi removido para análise histomorfométrica do número e diâmetro dos vasos sanguíneos através da coloração com Hematoxilina e Eosina (HE). O sangue recolhido foi centrifugado e o plasma foi utilizado para determinar os níveis de peroxidação lipídica mensurados pelas substâncias reativas ao ácido tiobarbitúrico (TBARS), vitamina C (VIT C) e glutationa reduzida (GSH). O status oxidativo demostrou maiores níveis de TBARS no grupo CP em 7, 15 e 30 dias, e indicou uma influência protetora da TFDa II no plasma observada a partir de menor peroxidação lipídica. Níveis de GSH foram consumidos nos grupos CP, TFDa I e TFDa II durante o experimento. Ainda, TFDa II também aumentou as defesas antioxidantes no plasma: a) níveis mais elevados de GSH, e b) aumento dos níveis de VIT C. Interessantemente, os níveis plasmáticos de VIT C foram restaurados no grupo TFDa II no trigésimo dia experimental. Os achados histomorfométricos mostraram em 7 dias que os grupos tratados (RAR, TFDa I e TFDa II) apresentaram elevado número de vasos sanguíneos, e o grupo TFDa II apresentou os maiores valores entre eles. A partir destes resultados, fica evidente que TFDa modifica a DP, reduzindo o dano oxidativo sistêmico, e estimula o sistema de defesa antioxidante, protegendo, assim, as zonas afetadas intimamente pela DP em ratos. Além disso, foi observado uma relação entre a maior expressão da angiogênese e o estágio de reparo da DP. Em síntese, sugerimos que a TFDa com AM solubilizado em etanol proporciona melhores respostas terapêuticas no tratamento periodontal.Universidade Federal de Santa MariaBROdontologiaUFSMPrograma de Pós-Graduação em Ciências OdontológicasDanesi, Cristiane Cademartorihttp://lattes.cnpq.br/9106848911495258Barcelos, Raquel Cristine Silvahttp://lattes.cnpq.br/3355023039056051Payeras, Márcia Rodrigueshttp://lattes.cnpq.br/6822386948620231Barin, Luisa Machado2016-02-172016-02-172015-07-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfBARIN, Luisa Machado. Role of the adjunctive antimicrobial photodynamic therapy to periodontal treatment at plasmatic oxidative stress parameters and vascular behavior. 2015. 73 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Santa Maria, Santa Maria, 2015.http://repositorio.ufsm.br/handle/1/6159porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-09-06T14:16:10Zoai:repositorio.ufsm.br:1/6159Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-09-06T14:16:10Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Papel da terapia fotodinâmica antimicrobiana adjuvante ao tratamento periodontal sob parâmetros de estresse oxidativo plasmático e comportamento vascular
Role of the adjunctive antimicrobial photodynamic therapy to periodontal treatment at plasmatic oxidative stress parameters and vascular behavior
title Papel da terapia fotodinâmica antimicrobiana adjuvante ao tratamento periodontal sob parâmetros de estresse oxidativo plasmático e comportamento vascular
spellingShingle Papel da terapia fotodinâmica antimicrobiana adjuvante ao tratamento periodontal sob parâmetros de estresse oxidativo plasmático e comportamento vascular
Barin, Luisa Machado
Azul de metileno
Estresse oxidativo
Etanol
Neovascularização
Terapia fotodinâmica antimicrobiana
Antimicrobial photodynamic therapy
Ethanol
Methylene blue
Neovascularization
Oxidative stress
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
title_short Papel da terapia fotodinâmica antimicrobiana adjuvante ao tratamento periodontal sob parâmetros de estresse oxidativo plasmático e comportamento vascular
title_full Papel da terapia fotodinâmica antimicrobiana adjuvante ao tratamento periodontal sob parâmetros de estresse oxidativo plasmático e comportamento vascular
title_fullStr Papel da terapia fotodinâmica antimicrobiana adjuvante ao tratamento periodontal sob parâmetros de estresse oxidativo plasmático e comportamento vascular
title_full_unstemmed Papel da terapia fotodinâmica antimicrobiana adjuvante ao tratamento periodontal sob parâmetros de estresse oxidativo plasmático e comportamento vascular
title_sort Papel da terapia fotodinâmica antimicrobiana adjuvante ao tratamento periodontal sob parâmetros de estresse oxidativo plasmático e comportamento vascular
author Barin, Luisa Machado
author_facet Barin, Luisa Machado
author_role author
dc.contributor.none.fl_str_mv Danesi, Cristiane Cademartori
http://lattes.cnpq.br/9106848911495258
Barcelos, Raquel Cristine Silva
http://lattes.cnpq.br/3355023039056051
Payeras, Márcia Rodrigues
http://lattes.cnpq.br/6822386948620231
dc.contributor.author.fl_str_mv Barin, Luisa Machado
dc.subject.por.fl_str_mv Azul de metileno
Estresse oxidativo
Etanol
Neovascularização
Terapia fotodinâmica antimicrobiana
Antimicrobial photodynamic therapy
Ethanol
Methylene blue
Neovascularization
Oxidative stress
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
topic Azul de metileno
Estresse oxidativo
Etanol
Neovascularização
Terapia fotodinâmica antimicrobiana
Antimicrobial photodynamic therapy
Ethanol
Methylene blue
Neovascularization
Oxidative stress
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
description The aim of this study was to evaluate the effects of methylene blue (MB) photosensitizer (PS) solubilized in ethanol in antimicrobial photodynamic therapy (aPDT) as an adjuvant periodontal treatment, at oxidative stress (OS) parameters and vascular behavior in rat model. Besides, investigate greater involvement of angiogenesis at the destruction or healing stages of periodontal disease (PD). One hundred and twenty male adult Wistar rats were divided randomly into five groups. The control groups were negative control (NC) (no PD, n=15) and positive control (PC) (with PD, without any treatment, n=15). The other groups had PD and underwent different types of treatment, such as scaling and root planing (SRP) and irrigation with 1mL of saline solution (SRP, n=30); SRP and aPDT with MB solubilized in water (aPDT I, n=30); SRP and aPDT with MB containing ethanol (aPDT II, n=30). The PD was induced via the installation of a ligature around the mandibular right first molar. After 7 days, the ligature was removed and animals received treatment. At 7, 15 and 30 days, rats were euthanized and the gingival tissue surrounding the induction area was removed for histomorphometric analysis of the number and diameter of blood vessels by staining with hematoxylin and eosin (H&E). The collected blood was centrifuged and the plasma was used to determine lipid peroxidation by quantifying thiobarbituric acid reactive substances (TBARS), vitamin C (VIT C) and glutathione reduced (GSH) levels. The oxidative status showed higher TBARS levels in PC group in 7, 15 and 30 days, and indicated a protective influence of aPDT II on plasma observed from lower lipid peroxidation. GSH levels were consumed in PC, aPDT I and aPDT II groups throughout the experiment. Furthermore, aPDT II also increased antioxidant defenses in plasma: i) higher levels of GSH, and ii) increased levels of VIT C. Interestingly, the VIT C plasmatic levels were restored in the aPDT II group in the 30th experimental day. Histomorphometric findings in 7 days showed that treated groups (SRP, aPDT I and aPDT II) showed higher number of blood vessels, and the aPDT II group showed the highest values among them. From these results, aPDT modifies PD course, reducing oxidative systemic damage and stimulating the antioxidant defense system, thus protecting the areas closely affected by PD in rats. Moreover, was observed a relationship between increased expression of angiogenesis and repair stage of the PD. In summary, we suggest that the aPDT with MB solubilized in ethanol provides better therapeutic responses in periodontal treatment.
publishDate 2015
dc.date.none.fl_str_mv 2015-07-16
2016-02-17
2016-02-17
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv BARIN, Luisa Machado. Role of the adjunctive antimicrobial photodynamic therapy to periodontal treatment at plasmatic oxidative stress parameters and vascular behavior. 2015. 73 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Santa Maria, Santa Maria, 2015.
http://repositorio.ufsm.br/handle/1/6159
identifier_str_mv BARIN, Luisa Machado. Role of the adjunctive antimicrobial photodynamic therapy to periodontal treatment at plasmatic oxidative stress parameters and vascular behavior. 2015. 73 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Santa Maria, Santa Maria, 2015.
url http://repositorio.ufsm.br/handle/1/6159
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Odontologia
UFSM
Programa de Pós-Graduação em Ciências Odontológicas
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Odontologia
UFSM
Programa de Pós-Graduação em Ciências Odontológicas
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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