Quantificação de α-tocoferol, retinol e carotenóides e seus possíveis efeitos sobre a peroxidação lipídica em trabalhadores expostos a solventes
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/8997 |
Resumo: | Oxidative stress is a process characterized by the antioxidant defense system decrease and/or an excessive reactive species (RS) production. RS are substances capable of attacking proteins, lipids and DNA. The oxidative damage caused to lipids is known as lipid peroxidation, which leads to the increase of malondialdehyde (MDA) levels. Oxidative stress is involved in the pathogenesis of many chronic diseases, including diabetes, cancer, Parkinson s disease and damaged tissue in exposed to chemical agents, such as neurotoxicity, hematotoxicity and nefrotoxicity. It is known that there is a close relation between organic solvents, present in paints, and oxidative stress. Thus, the body has an elaborate antioxidant defense system, the exogenous antioxidants, such as lipid soluble vitamins, and the endogenous system, such as antioxidant enzymes. In this study a method has been validated and optimized for simultaneous quantification of retinol, α-tocopherol, lycopene and β- carotene using high performance liquid chromatography (HPLC-UV/fluorescence). The analytical parameters of validation analyzed were linearity, precision, accuracy, recovery and limits of detection (LOD) and quantification (LOQ). For all the vitamins analyzed, the linear regression coefficients were > 0.99, CV% < 5%;% bias < ± 6% recovery > 92% and the LOD and LOQ values obtained were satisfactory for routine clinical application for all analytes. The validated method was applied to a group of occupationally exposed to paints (n = 45) and a non-exposed group (control group, n = 30). The results indicated that all vitamins, except vitamin E, were significantly lower in the exposed group. Moreover, the possible correlation between endogenous and exogenous antioxidants and lipid damage was evaluated. Quantifications were done to assess endogenous antioxidants, reduced glutathione (GSH) in erythrocytes, the enzymes superoxide dismutase (SOD) and catalase (CAT) in whole blood through spectroscopic methods, and malondialdehyde (MDA) levels in plasma through HPLC-VIS in both study groups, exposed (n=42) and controls (n=28). The biological monitoring was performed by measurement of blood toluene, since in previous studies, it was suggested that this solvent would be the major inducer of lipid peroxidation. Despite the low levels of toluene found, exposed workers presented higher levels of MDA and the antioxidant enzymes (SOD and CAT) activities were significantly elevated when compared with the control group; and this increase was accompanied by depletion of GSH levels. Also, several correlations were observed between MDA and the enzymatic (SOD and CAT) and non-enzymatic antioxidants (GSH), and with lipid-soluble vitamins as well, except vitamin E. Through statistical tests the antioxidants which have a greater influence on the levels of MDA were evaluated. Among the antioxidants tested, GSH and carotenoids (mainly β- carotene) were suggested as main responsible for the reduction of lipid peroxidation. Thus, it can be suggested that high intakes of exogenous antioxidants, such as carotenoids,, tend to decrease lipid damage in occupationally exposed individuals to solvents constituent of paints. |
id |
UFSM_532db5c4cec9bb6a529d54c9cca20784 |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/8997 |
network_acronym_str |
UFSM |
network_name_str |
Manancial - Repositório Digital da UFSM |
repository_id_str |
|
spelling |
Quantificação de α-tocoferol, retinol e carotenóides e seus possíveis efeitos sobre a peroxidação lipídica em trabalhadores expostos a solventesQuantification of α-tocopherol, retinol e carotenoids and their possibles effects on lipid peroxidation in workers exposed to solventsVitaminas lipossolúveisExposição ocupacionalPeroxidação lipídicaEstresse oxidativoAntioxidantesLipid-soluble vitaminsOccupational exposureLipid peroxidationOxidative stressAntioxidantsCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIAOxidative stress is a process characterized by the antioxidant defense system decrease and/or an excessive reactive species (RS) production. RS are substances capable of attacking proteins, lipids and DNA. The oxidative damage caused to lipids is known as lipid peroxidation, which leads to the increase of malondialdehyde (MDA) levels. Oxidative stress is involved in the pathogenesis of many chronic diseases, including diabetes, cancer, Parkinson s disease and damaged tissue in exposed to chemical agents, such as neurotoxicity, hematotoxicity and nefrotoxicity. It is known that there is a close relation between organic solvents, present in paints, and oxidative stress. Thus, the body has an elaborate antioxidant defense system, the exogenous antioxidants, such as lipid soluble vitamins, and the endogenous system, such as antioxidant enzymes. In this study a method has been validated and optimized for simultaneous quantification of retinol, α-tocopherol, lycopene and β- carotene using high performance liquid chromatography (HPLC-UV/fluorescence). The analytical parameters of validation analyzed were linearity, precision, accuracy, recovery and limits of detection (LOD) and quantification (LOQ). For all the vitamins analyzed, the linear regression coefficients were > 0.99, CV% < 5%;% bias < ± 6% recovery > 92% and the LOD and LOQ values obtained were satisfactory for routine clinical application for all analytes. The validated method was applied to a group of occupationally exposed to paints (n = 45) and a non-exposed group (control group, n = 30). The results indicated that all vitamins, except vitamin E, were significantly lower in the exposed group. Moreover, the possible correlation between endogenous and exogenous antioxidants and lipid damage was evaluated. Quantifications were done to assess endogenous antioxidants, reduced glutathione (GSH) in erythrocytes, the enzymes superoxide dismutase (SOD) and catalase (CAT) in whole blood through spectroscopic methods, and malondialdehyde (MDA) levels in plasma through HPLC-VIS in both study groups, exposed (n=42) and controls (n=28). The biological monitoring was performed by measurement of blood toluene, since in previous studies, it was suggested that this solvent would be the major inducer of lipid peroxidation. Despite the low levels of toluene found, exposed workers presented higher levels of MDA and the antioxidant enzymes (SOD and CAT) activities were significantly elevated when compared with the control group; and this increase was accompanied by depletion of GSH levels. Also, several correlations were observed between MDA and the enzymatic (SOD and CAT) and non-enzymatic antioxidants (GSH), and with lipid-soluble vitamins as well, except vitamin E. Through statistical tests the antioxidants which have a greater influence on the levels of MDA were evaluated. Among the antioxidants tested, GSH and carotenoids (mainly β- carotene) were suggested as main responsible for the reduction of lipid peroxidation. Thus, it can be suggested that high intakes of exogenous antioxidants, such as carotenoids,, tend to decrease lipid damage in occupationally exposed individuals to solvents constituent of paints.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorO estresse oxidativo é um processo caracterizado pela diminuição do sistema de defesa antioxidante e/ou por uma produção excessiva de espécies reativas (ERs). As ERs são substâncias capazes de lesar proteínas, lipídios e DNA. Quando os lipídios são atingidos ocorre um processo chamado de peroxidação lipídica que leva ao aumento nos níveis de malondialdeído (MDA). O estresse oxidativo está envolvido com a patogênese de muitas doenças crônicas, como diabetes, câncer, doença de Parkinson e em danos teciduais em expostos a agentes químicos, como neurotoxicidade, hematotoxicidade e nefrotoxicidade. Sabe-se que existe uma estreita relação entre os solventes orgânicos, presentes em tintas, e o estresse oxidativo. Diante disso, o organismo dispõe de um elaborado sistema de defesa antioxidante, os antioxidantes exógenos, como as vitaminas lipossolúveis e o sistema endógeno como enzimas antioxidantes. Dessa forma, nesse estudo foi primeiramente otimizada e validada metodologia para simultânea quantificação de antioxidantes exógenos: retinol, α-tocoferol, licopeno e β-caroteno, utilizando cromatografia líquida de alta eficiência (CLAE-VIS/fluorescência). Os parâmetros analíticos de validação analisados foram linearidade, precisão, exatidão, recuperação e limites de detecção (LD) e quantificação (LQ). Para todas as vitaminas analisadas, os coeficientes de regressão linear foram > 0,99; CV% < 5%; bias% < ± 6%; recuperação > 92% e os valores de LD e LQ obtidos foram satisfatórios para aplicação na rotina clínica. O método validado foi aplicado em um grupo de expostos ocupacionalmente a tintas (n=45) e um grupo de não expostos (controle, n=30). Os resultados indicaram que todas as vitaminas, exceto a vitamina E, foram significativamente menores no grupo exposto. Além disso, avaliou-se o possível efeito protetivo de antioxidantes exógenos e endógenos sobre o dano lipídico. Foram realizadas as dosagens dos antioxidantes endógenos, glutationa reduzida (GSH) em eritrócitos, das enzimas superóxido dismutase (SOD) e catalase (CAT) em sangue total por métodos espectrofotométricos e os níveis de malondialdeído (MDA) em plasma por CLAE-VIS nos dois grupos de estudo, expostos (n=42) e controles (n=28). A monitorização biológica foi realizada através da dosagem de tolueno sanguíneo, uma vez que, em trabalhos prévios, foi sugerido que este solvente seria o principal indutor de peroxidação lipídica. Apesar dos baixos níveis de tolueno sanguíneo encontrados, os trabalhadores expostos apresentaram níveis de MDA e atividade das enzimas antioxidantes (SOD e CAT) significativamente elevados, quando comparados com o grupo controle e esse aumento foi acompanhado de depleção nos níveis de GSH. Ainda, foram observadas várias correlações entre os níveis de MDA e os antioxidantes endógenos enzimáticos (SOD e CAT) e não enzimático (GSH) e ainda com as vitaminas lipossolúveis, exceto vitamina E. Através de testes estatísticos foram avaliados quais antioxidantes teriam uma maior influência nos níveis de MDA. Dentre os antioxidantes analisados, a GSH e os carotenóides (principalmente o β- caroteno) foram sugeridos como principais responsáveis pela redução da peroxidação lipídica. Com isso, pode-se sugerir que aumento nos níveis de carotenóides, via dieta, tendem a diminuir o dano lipídico em indivíduos ocupacionalmente expostos a solventes constituintes de tintas.Universidade Federal de Santa MariaBRFarmacologiaUFSMPrograma de Pós-Graduação em FarmacologiaGarcia, Solange Cristinahttp://lattes.cnpq.br/6687355709603379Cardoso, Simone Gonçalveshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4595183J5Gioda, Adrianahttp://lattes.cnpq.br/3948737758921845Charão, Mariele Feiffer2015-01-282015-01-282011-02-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfCHARÃO, Mariele Feiffer. QUANTIFICATION OF α-TOCOPHEROL, RETINOL E CAROTENOIDS AND THEIR POSSIBLES EFFECTS ON LIPID PEROXIDATION IN WORKERS EXPOSED TO SOLVENTS. 2011. 134 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/8997porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2021-09-21T18:03:32Zoai:repositorio.ufsm.br:1/8997Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2021-09-21T18:03:32Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Quantificação de α-tocoferol, retinol e carotenóides e seus possíveis efeitos sobre a peroxidação lipídica em trabalhadores expostos a solventes Quantification of α-tocopherol, retinol e carotenoids and their possibles effects on lipid peroxidation in workers exposed to solvents |
title |
Quantificação de α-tocoferol, retinol e carotenóides e seus possíveis efeitos sobre a peroxidação lipídica em trabalhadores expostos a solventes |
spellingShingle |
Quantificação de α-tocoferol, retinol e carotenóides e seus possíveis efeitos sobre a peroxidação lipídica em trabalhadores expostos a solventes Charão, Mariele Feiffer Vitaminas lipossolúveis Exposição ocupacional Peroxidação lipídica Estresse oxidativo Antioxidantes Lipid-soluble vitamins Occupational exposure Lipid peroxidation Oxidative stress Antioxidants CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
title_short |
Quantificação de α-tocoferol, retinol e carotenóides e seus possíveis efeitos sobre a peroxidação lipídica em trabalhadores expostos a solventes |
title_full |
Quantificação de α-tocoferol, retinol e carotenóides e seus possíveis efeitos sobre a peroxidação lipídica em trabalhadores expostos a solventes |
title_fullStr |
Quantificação de α-tocoferol, retinol e carotenóides e seus possíveis efeitos sobre a peroxidação lipídica em trabalhadores expostos a solventes |
title_full_unstemmed |
Quantificação de α-tocoferol, retinol e carotenóides e seus possíveis efeitos sobre a peroxidação lipídica em trabalhadores expostos a solventes |
title_sort |
Quantificação de α-tocoferol, retinol e carotenóides e seus possíveis efeitos sobre a peroxidação lipídica em trabalhadores expostos a solventes |
author |
Charão, Mariele Feiffer |
author_facet |
Charão, Mariele Feiffer |
author_role |
author |
dc.contributor.none.fl_str_mv |
Garcia, Solange Cristina http://lattes.cnpq.br/6687355709603379 Cardoso, Simone Gonçalves http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4595183J5 Gioda, Adriana http://lattes.cnpq.br/3948737758921845 |
dc.contributor.author.fl_str_mv |
Charão, Mariele Feiffer |
dc.subject.por.fl_str_mv |
Vitaminas lipossolúveis Exposição ocupacional Peroxidação lipídica Estresse oxidativo Antioxidantes Lipid-soluble vitamins Occupational exposure Lipid peroxidation Oxidative stress Antioxidants CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
topic |
Vitaminas lipossolúveis Exposição ocupacional Peroxidação lipídica Estresse oxidativo Antioxidantes Lipid-soluble vitamins Occupational exposure Lipid peroxidation Oxidative stress Antioxidants CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
description |
Oxidative stress is a process characterized by the antioxidant defense system decrease and/or an excessive reactive species (RS) production. RS are substances capable of attacking proteins, lipids and DNA. The oxidative damage caused to lipids is known as lipid peroxidation, which leads to the increase of malondialdehyde (MDA) levels. Oxidative stress is involved in the pathogenesis of many chronic diseases, including diabetes, cancer, Parkinson s disease and damaged tissue in exposed to chemical agents, such as neurotoxicity, hematotoxicity and nefrotoxicity. It is known that there is a close relation between organic solvents, present in paints, and oxidative stress. Thus, the body has an elaborate antioxidant defense system, the exogenous antioxidants, such as lipid soluble vitamins, and the endogenous system, such as antioxidant enzymes. In this study a method has been validated and optimized for simultaneous quantification of retinol, α-tocopherol, lycopene and β- carotene using high performance liquid chromatography (HPLC-UV/fluorescence). The analytical parameters of validation analyzed were linearity, precision, accuracy, recovery and limits of detection (LOD) and quantification (LOQ). For all the vitamins analyzed, the linear regression coefficients were > 0.99, CV% < 5%;% bias < ± 6% recovery > 92% and the LOD and LOQ values obtained were satisfactory for routine clinical application for all analytes. The validated method was applied to a group of occupationally exposed to paints (n = 45) and a non-exposed group (control group, n = 30). The results indicated that all vitamins, except vitamin E, were significantly lower in the exposed group. Moreover, the possible correlation between endogenous and exogenous antioxidants and lipid damage was evaluated. Quantifications were done to assess endogenous antioxidants, reduced glutathione (GSH) in erythrocytes, the enzymes superoxide dismutase (SOD) and catalase (CAT) in whole blood through spectroscopic methods, and malondialdehyde (MDA) levels in plasma through HPLC-VIS in both study groups, exposed (n=42) and controls (n=28). The biological monitoring was performed by measurement of blood toluene, since in previous studies, it was suggested that this solvent would be the major inducer of lipid peroxidation. Despite the low levels of toluene found, exposed workers presented higher levels of MDA and the antioxidant enzymes (SOD and CAT) activities were significantly elevated when compared with the control group; and this increase was accompanied by depletion of GSH levels. Also, several correlations were observed between MDA and the enzymatic (SOD and CAT) and non-enzymatic antioxidants (GSH), and with lipid-soluble vitamins as well, except vitamin E. Through statistical tests the antioxidants which have a greater influence on the levels of MDA were evaluated. Among the antioxidants tested, GSH and carotenoids (mainly β- carotene) were suggested as main responsible for the reduction of lipid peroxidation. Thus, it can be suggested that high intakes of exogenous antioxidants, such as carotenoids,, tend to decrease lipid damage in occupationally exposed individuals to solvents constituent of paints. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02-18 2015-01-28 2015-01-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
CHARÃO, Mariele Feiffer. QUANTIFICATION OF α-TOCOPHEROL, RETINOL E CAROTENOIDS AND THEIR POSSIBLES EFFECTS ON LIPID PEROXIDATION IN WORKERS EXPOSED TO SOLVENTS. 2011. 134 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2011. http://repositorio.ufsm.br/handle/1/8997 |
identifier_str_mv |
CHARÃO, Mariele Feiffer. QUANTIFICATION OF α-TOCOPHEROL, RETINOL E CAROTENOIDS AND THEIR POSSIBLES EFFECTS ON LIPID PEROXIDATION IN WORKERS EXPOSED TO SOLVENTS. 2011. 134 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
url |
http://repositorio.ufsm.br/handle/1/8997 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Farmacologia UFSM Programa de Pós-Graduação em Farmacologia |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Farmacologia UFSM Programa de Pós-Graduação em Farmacologia |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922088672821248 |