Determinação multirresíduo de agrotóxicos em bebidas à base de soja empregando método QuEChERS e UHPLC-MS/MS

• Autor(a) principal: May, Marília Melina
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Manancial - Repositório Digital da UFSM
• Texto Completo: http://repositorio.ufsm.br/handle/1/10617
• topic: Bebidas à base de soja; Agrotóxicos; QuEChERS; Planejamento experimental; UHPLC-MS/MS; Soy-based beverages; Pesticides; QuEChERS; Experimental design; UHPLC-MS/MS; CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA

Soy beverages are aqueous extracts of soybeans which are a source of high quality protein and no contains cholesterol or lactose, can be considered functional foods. These beverages have a large acceptance among consumers and due to they are extracted from the grain, the beneficial components are preserved, but may also contain residues of pesticides used to control pest infestation during the growing of the grains. This study aimed to develop and validate a multiresidue method based on QuEChERS extraction for the simultaneous determination of 41 pesticides in soy-based beverages using ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). In the sample preparation step, it was used 10 mL of sample and 10 mL of acetonitrile and addition of salts. The clean-up procedure was optimized by experimental design, evaluating different quantities of the sorbents primary secondary amine (PSA) and octadecylsilane (C18).The clean-up step was performed with magnesium sulfate anhydrous and C18. To assess the accuracy of the method, blank samples were spiked at 10, 25 and 50 μg L-1 with n = 6. To evaluate the matrix effect, inclinations of curves in acetonitrile and blank extract of the matrix were compared. The results of the validation are satisfactory, since the method presented optimized recoveries between 70 and 112% with RSD less than 19%. Curves were prepared in the matrix extract, due to some compounds presented pronounced matrix effect. The curves showed r² ≥ 0,99 for all compounds in the range between the respective limit of quantification and 50 μg L-1. The method proved to be suitable for the determination of the compounds and may be used for routine analysis.