Controle biológico (Glomus etunicatum), químico (fipronil) e estudo molecular (PCR-ITS) do nematóide do cisto da soja
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/5530 |
Resumo: | The studies of cist nematodes were evaluated in the greenhouse condition and in the Prof. Marcos Rubens Fries Environment and Soil Microbiology and Biology Laboratory, in the Soil Department of the Federal University of Santa Maria, in three stages. The first stage aimed to study through a survey of the presence of cyst nematodeos in soybean (NCS), in five counties, in Rio Grande do Sul state. The survey was carried out in eigth place with 28 samples with soybean culture. The counties were Espumoso, Eugênio de Castro, São Miguel das Missões and Tupanciretã. The soil samples had been processed in laboratory, and the NCS had been extracted by wet technique. It was detected presence of NCS in all five studied counties. The highest number of cysts was found in the counties of Espumoso and São Miguel das Missões. The second stage of the aimed to study the suppression of the NCS population using plants and to test the action of the fipronil on the egg hatching of NCS. Two experiments had been developed, the first with 10 treatments (without eggs of NCS and mycorrhizal, four treatments with diferents levels of eggs of NCS, 500, 1000, 2000 e 4000 eggs; with mycorrhizal; four treatment with mycorrhizal and eggs of NCS). In this experiment was observed that the presence of Glomus etunicatum fungi improved the plant grow. The study with fipronil showed significant reduction in hacthing when the eggs were exposed to the diferent concentrations of fipronil. It was observed, that the addition of small levels of fipronil can reduce the hatching in 50%. The third stage of the work was to evaluate the DNA characterization of the Heterodera glycines cists. It was obtained a good DNA extraction and experiments are carring out to do the PCR-ITS studies. |
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Controle biológico (Glomus etunicatum), químico (fipronil) e estudo molecular (PCR-ITS) do nematóide do cisto da sojaBiological (Glomus etunicatum), chemistry (fipronil) control and molecular (PCR-ITS) studies of cist cematodes in soybeanAgriculturaSojaNematóidesControle biológicoCNPQ::CIENCIAS AGRARIAS::AGRONOMIAThe studies of cist nematodes were evaluated in the greenhouse condition and in the Prof. Marcos Rubens Fries Environment and Soil Microbiology and Biology Laboratory, in the Soil Department of the Federal University of Santa Maria, in three stages. The first stage aimed to study through a survey of the presence of cyst nematodeos in soybean (NCS), in five counties, in Rio Grande do Sul state. The survey was carried out in eigth place with 28 samples with soybean culture. The counties were Espumoso, Eugênio de Castro, São Miguel das Missões and Tupanciretã. The soil samples had been processed in laboratory, and the NCS had been extracted by wet technique. It was detected presence of NCS in all five studied counties. The highest number of cysts was found in the counties of Espumoso and São Miguel das Missões. The second stage of the aimed to study the suppression of the NCS population using plants and to test the action of the fipronil on the egg hatching of NCS. Two experiments had been developed, the first with 10 treatments (without eggs of NCS and mycorrhizal, four treatments with diferents levels of eggs of NCS, 500, 1000, 2000 e 4000 eggs; with mycorrhizal; four treatment with mycorrhizal and eggs of NCS). In this experiment was observed that the presence of Glomus etunicatum fungi improved the plant grow. The study with fipronil showed significant reduction in hacthing when the eggs were exposed to the diferent concentrations of fipronil. It was observed, that the addition of small levels of fipronil can reduce the hatching in 50%. The third stage of the work was to evaluate the DNA characterization of the Heterodera glycines cists. It was obtained a good DNA extraction and experiments are carring out to do the PCR-ITS studies.O trabalho foi conduzido em casa de vegetação e no laboratório de Microbiologia e Biologia do Solo e do Ambiente Prof. Marcos Rubens Fries, do Departamento de Solos da Universidade Federal de Santa Maria, em três etapas. A primeira etapa objetivou realizar um levantamento da presença do nematóide de cisto da soja (NCS) em cinco municípios produtores de soja no estado do Rio Grande do Sul. O levantamento foi conduzido em oito diferentes áreas com cultivo da soja, tendo 28 locais de coleta de amostras, nos municípios de Espumoso, Eugênio de Castro, Jóia, São Miguel das Missões e Tupanciretã. As amostras de solo foram processadas em laboratório e os cistos do NCS foram extraídos pela técnica de peneiramento úmido. Detectou-se a presença do nematóide nos cinco municípios estudados, sendo que o maior número de cistos foi encontrado nos municípios de Espumoso e São Miguel das Missões. A segunda etapa da pesquisa objetivou avaliar a supressão da população do nematóide do cisto da soja através de plantas micorrizadas e testar a ação do fipronil na eclosão de juvenis do nematóide. Dois experimentos foram desenvolvidos. O primeiro foi composto por 10 tratamentos (testemunha, quatro tratamentos com ovos do NCS, 500, 1000, 2000 e 4000 sem micorriza; quatro tratamentos com micorriza e ovos de NCS). Neste experimento observou-se que a presença de Glomus etunicatum beneficiou o crescimento da planta. O segundo constituiu-se de experimento com fipronil e mostrou uma significativa redução na eclosão dos ovos de NCS. Observouse que a adição de pequenas doses do fipronil foi capaz de diminuir a eclosão dos juvenis do NCS em 50%. A terceira etapa do trabalho teve por objetivo testar a caracterização do NCS via extração de DNA e técnica de PCR-ITS. Nesta etapa foi obtida uma boa extração de DNA dos cistos de Heterodera glycines e estudos estão sendo realizados para efetuar a caracterização via PCR-ITS.Universidade Federal de Santa MariaBRAgronomiaUFSMPrograma de Pós-Graduação em Ciência do SoloAntoniolli, Zaida Ineshttp://lattes.cnpq.br/4692942549618168Muniz, Marlove Fátima Briãohttp://lattes.cnpq.br/3148312031889388Bertagnolli, Paulo Fernandohttp://lattes.cnpq.br/3164927231278535Benedetti, Tatiana2017-04-112017-04-112005-02-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfBENEDETTI, Tatiana. Biological (Glomus etunicatum), chemistry (fipronil) control and molecular (PCR-ITS) studies of cist cematodes in soybean. 2005. 57 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2005.http://repositorio.ufsm.br/handle/1/5530porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-03-18T19:16:57Zoai:repositorio.ufsm.br:1/5530Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-03-18T19:16:57Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Controle biológico (Glomus etunicatum), químico (fipronil) e estudo molecular (PCR-ITS) do nematóide do cisto da soja Biological (Glomus etunicatum), chemistry (fipronil) control and molecular (PCR-ITS) studies of cist cematodes in soybean |
title |
Controle biológico (Glomus etunicatum), químico (fipronil) e estudo molecular (PCR-ITS) do nematóide do cisto da soja |
spellingShingle |
Controle biológico (Glomus etunicatum), químico (fipronil) e estudo molecular (PCR-ITS) do nematóide do cisto da soja Benedetti, Tatiana Agricultura Soja Nematóides Controle biológico CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Controle biológico (Glomus etunicatum), químico (fipronil) e estudo molecular (PCR-ITS) do nematóide do cisto da soja |
title_full |
Controle biológico (Glomus etunicatum), químico (fipronil) e estudo molecular (PCR-ITS) do nematóide do cisto da soja |
title_fullStr |
Controle biológico (Glomus etunicatum), químico (fipronil) e estudo molecular (PCR-ITS) do nematóide do cisto da soja |
title_full_unstemmed |
Controle biológico (Glomus etunicatum), químico (fipronil) e estudo molecular (PCR-ITS) do nematóide do cisto da soja |
title_sort |
Controle biológico (Glomus etunicatum), químico (fipronil) e estudo molecular (PCR-ITS) do nematóide do cisto da soja |
author |
Benedetti, Tatiana |
author_facet |
Benedetti, Tatiana |
author_role |
author |
dc.contributor.none.fl_str_mv |
Antoniolli, Zaida Ines http://lattes.cnpq.br/4692942549618168 Muniz, Marlove Fátima Brião http://lattes.cnpq.br/3148312031889388 Bertagnolli, Paulo Fernando http://lattes.cnpq.br/3164927231278535 |
dc.contributor.author.fl_str_mv |
Benedetti, Tatiana |
dc.subject.por.fl_str_mv |
Agricultura Soja Nematóides Controle biológico CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
topic |
Agricultura Soja Nematóides Controle biológico CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
The studies of cist nematodes were evaluated in the greenhouse condition and in the Prof. Marcos Rubens Fries Environment and Soil Microbiology and Biology Laboratory, in the Soil Department of the Federal University of Santa Maria, in three stages. The first stage aimed to study through a survey of the presence of cyst nematodeos in soybean (NCS), in five counties, in Rio Grande do Sul state. The survey was carried out in eigth place with 28 samples with soybean culture. The counties were Espumoso, Eugênio de Castro, São Miguel das Missões and Tupanciretã. The soil samples had been processed in laboratory, and the NCS had been extracted by wet technique. It was detected presence of NCS in all five studied counties. The highest number of cysts was found in the counties of Espumoso and São Miguel das Missões. The second stage of the aimed to study the suppression of the NCS population using plants and to test the action of the fipronil on the egg hatching of NCS. Two experiments had been developed, the first with 10 treatments (without eggs of NCS and mycorrhizal, four treatments with diferents levels of eggs of NCS, 500, 1000, 2000 e 4000 eggs; with mycorrhizal; four treatment with mycorrhizal and eggs of NCS). In this experiment was observed that the presence of Glomus etunicatum fungi improved the plant grow. The study with fipronil showed significant reduction in hacthing when the eggs were exposed to the diferent concentrations of fipronil. It was observed, that the addition of small levels of fipronil can reduce the hatching in 50%. The third stage of the work was to evaluate the DNA characterization of the Heterodera glycines cists. It was obtained a good DNA extraction and experiments are carring out to do the PCR-ITS studies. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-02-25 2017-04-11 2017-04-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BENEDETTI, Tatiana. Biological (Glomus etunicatum), chemistry (fipronil) control and molecular (PCR-ITS) studies of cist cematodes in soybean. 2005. 57 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2005. http://repositorio.ufsm.br/handle/1/5530 |
identifier_str_mv |
BENEDETTI, Tatiana. Biological (Glomus etunicatum), chemistry (fipronil) control and molecular (PCR-ITS) studies of cist cematodes in soybean. 2005. 57 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2005. |
url |
http://repositorio.ufsm.br/handle/1/5530 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Agronomia UFSM Programa de Pós-Graduação em Ciência do Solo |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Agronomia UFSM Programa de Pós-Graduação em Ciência do Solo |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922106863517696 |