Metabolismo de nucleotídeos e nucleosídeo da adenina em linfócitos e plaquetas de pacientes com hepatite C
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/6039 |
Resumo: | Hepatitis C is an infectious disease caused by Hepatitis C virus (HCV) and characterized by development of inflammation as well as fibrosis. On the other hand, the extracellular nucleotides and adenine nucleosides are important signaling molecules that can modulate inflammatory responses in platelets and lymphocytes. This study sought to determine the activities of ectoenzymes [ectonucleoside triphosphate diphosphohydrolase (E-NTPDase), ecto-5 -nucleotidase (E-5 -NT) and ecto-adenosine deaminase (E-ADA)] that metabolize nucleotides in platelets and lymphocytes and to evaluate cytokine levels of patients with HCV. Twenty five HCV patients and fifty healthy subjects (control group) were selected for this study. In lymphocytes, was observed a lower ATP hydrolysis (P<0.001), and higher ADP hydrolysis (P<0.001) and E- ADA activity (P<0.001) in HCV patients when compared to the control group. In addition, the results revealed a higher ATP and ADP hydrolysis by E-NTPDase and higher E-ADA activity in platelets of HCV patients when compared with the control group. Also, IL-4 and IL-10 were significantly (P<0.05) increased while IL-6 was decreased (P<0.05) in HCV patients. In conclusion, the results showed that the activities of E-NTPDase and E-ADA were altered in both lymphocytes and platelets of HCV patients. These alterations in enzymes activities may be possible regulatory mechanisms in an attempt to manage liver damage and ongoing inflammatory process. Furthermore, decreased IL-6 as well as increased IL-4 and IL-10 in HCV patients may suggest an antiinflammatory process and possible compensatory mechanisms in minimizing liver injury. |
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Metabolismo de nucleotídeos e nucleosídeo da adenina em linfócitos e plaquetas de pacientes com hepatite CMetabolism of nucleotide and nucleoside adenine in lymphocytes and platelets of patients with hepatitis CHepatite COs linfócitosPlaquetasEctoenzimasCitocinasSinalização purinérgicaHepatitis CLymphocytesPlateletsEctoenzymesCytokinesPurinergic signalingCNPQ::CIENCIAS DA SAUDE::FARMACIAHepatitis C is an infectious disease caused by Hepatitis C virus (HCV) and characterized by development of inflammation as well as fibrosis. On the other hand, the extracellular nucleotides and adenine nucleosides are important signaling molecules that can modulate inflammatory responses in platelets and lymphocytes. This study sought to determine the activities of ectoenzymes [ectonucleoside triphosphate diphosphohydrolase (E-NTPDase), ecto-5 -nucleotidase (E-5 -NT) and ecto-adenosine deaminase (E-ADA)] that metabolize nucleotides in platelets and lymphocytes and to evaluate cytokine levels of patients with HCV. Twenty five HCV patients and fifty healthy subjects (control group) were selected for this study. In lymphocytes, was observed a lower ATP hydrolysis (P<0.001), and higher ADP hydrolysis (P<0.001) and E- ADA activity (P<0.001) in HCV patients when compared to the control group. In addition, the results revealed a higher ATP and ADP hydrolysis by E-NTPDase and higher E-ADA activity in platelets of HCV patients when compared with the control group. Also, IL-4 and IL-10 were significantly (P<0.05) increased while IL-6 was decreased (P<0.05) in HCV patients. In conclusion, the results showed that the activities of E-NTPDase and E-ADA were altered in both lymphocytes and platelets of HCV patients. These alterations in enzymes activities may be possible regulatory mechanisms in an attempt to manage liver damage and ongoing inflammatory process. Furthermore, decreased IL-6 as well as increased IL-4 and IL-10 in HCV patients may suggest an antiinflammatory process and possible compensatory mechanisms in minimizing liver injury.Hepatitis C is an infectious disease caused by Hepatitis C virus (HCV) and characterized by development of inflammation as well as fibrosis. On the other hand, the extracellular nucleotides and adenine nucleosides are important signaling molecules that can modulate inflammatory responses in platelets and lymphocytes. This study sought to determine the activities of ectoenzymes [ectonucleoside triphosphate diphosphohydrolase (E-NTPDase), ecto-5 -nucleotidase (E-5 -NT) and ecto-adenosine deaminase (E-ADA)] that metabolize nucleotides in platelets and lymphocytes and to evaluate cytokine levels of patients with HCV. Twenty five HCV patients and fifty healthy subjects (control group) were selected for this study. In lymphocytes, was observed a lower ATP hydrolysis (P<0.001), and higher ADP hydrolysis (P<0.001) and E- ADA activity (P<0.001) in HCV patients when compared to the control group. In addition, the results revealed a higher ATP and ADP hydrolysis by E-NTPDase and higher E-ADA activity in platelets of HCV patients when compared with the control group. Also, IL-4 and IL-10 were significantly (P<0.05) increased while IL-6 was decreased (P<0.05) in HCV patients. In conclusion, the results showed that the activities of E-NTPDase and E-ADA were altered in both lymphocytes and platelets of HCV patients. These alterations in enzymes activities may be possible regulatory mechanisms in an attempt to manage liver damage and ongoing inflammatory process. Furthermore, decreased IL-6 as well as increased IL-4 and IL-10 in HCV patients may suggest an antiinflammatory process and possible compensatory mechanisms in minimizing liver injury.Universidade Federal de Santa MariaBRAnálises Clínicas e ToxicológicasUFSMPrograma de Pós-Graduação em Ciências FarmacêuticasLeal, Daniela Bitencourt Rosahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706487E7Silva, Jose Edson Paz dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799809H4Linares, Carlos Eduardo Blancohttp://lattes.cnpq.br/8135690917054350Basso, Maria Emilha2016-09-122016-09-122016-07-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfBASSO, Maria Emilha. METABOLISM OF NUCLEOTIDE AND NUCLEOSIDE ADENINE IN LYMPHOCYTES AND PLATELETS OF PATIENTS WITH HEPATITIS C. 2016. 69 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2016.http://repositorio.ufsm.br/handle/1/6039porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-10-10T17:13:11Zoai:repositorio.ufsm.br:1/6039Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-10-10T17:13:11Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Metabolismo de nucleotídeos e nucleosídeo da adenina em linfócitos e plaquetas de pacientes com hepatite C Metabolism of nucleotide and nucleoside adenine in lymphocytes and platelets of patients with hepatitis C |
title |
Metabolismo de nucleotídeos e nucleosídeo da adenina em linfócitos e plaquetas de pacientes com hepatite C |
spellingShingle |
Metabolismo de nucleotídeos e nucleosídeo da adenina em linfócitos e plaquetas de pacientes com hepatite C Basso, Maria Emilha Hepatite C Os linfócitos Plaquetas Ectoenzimas Citocinas Sinalização purinérgica Hepatitis C Lymphocytes Platelets Ectoenzymes Cytokines Purinergic signaling CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Metabolismo de nucleotídeos e nucleosídeo da adenina em linfócitos e plaquetas de pacientes com hepatite C |
title_full |
Metabolismo de nucleotídeos e nucleosídeo da adenina em linfócitos e plaquetas de pacientes com hepatite C |
title_fullStr |
Metabolismo de nucleotídeos e nucleosídeo da adenina em linfócitos e plaquetas de pacientes com hepatite C |
title_full_unstemmed |
Metabolismo de nucleotídeos e nucleosídeo da adenina em linfócitos e plaquetas de pacientes com hepatite C |
title_sort |
Metabolismo de nucleotídeos e nucleosídeo da adenina em linfócitos e plaquetas de pacientes com hepatite C |
author |
Basso, Maria Emilha |
author_facet |
Basso, Maria Emilha |
author_role |
author |
dc.contributor.none.fl_str_mv |
Leal, Daniela Bitencourt Rosa http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706487E7 Silva, Jose Edson Paz da http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799809H4 Linares, Carlos Eduardo Blanco http://lattes.cnpq.br/8135690917054350 |
dc.contributor.author.fl_str_mv |
Basso, Maria Emilha |
dc.subject.por.fl_str_mv |
Hepatite C Os linfócitos Plaquetas Ectoenzimas Citocinas Sinalização purinérgica Hepatitis C Lymphocytes Platelets Ectoenzymes Cytokines Purinergic signaling CNPQ::CIENCIAS DA SAUDE::FARMACIA |
topic |
Hepatite C Os linfócitos Plaquetas Ectoenzimas Citocinas Sinalização purinérgica Hepatitis C Lymphocytes Platelets Ectoenzymes Cytokines Purinergic signaling CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
Hepatitis C is an infectious disease caused by Hepatitis C virus (HCV) and characterized by development of inflammation as well as fibrosis. On the other hand, the extracellular nucleotides and adenine nucleosides are important signaling molecules that can modulate inflammatory responses in platelets and lymphocytes. This study sought to determine the activities of ectoenzymes [ectonucleoside triphosphate diphosphohydrolase (E-NTPDase), ecto-5 -nucleotidase (E-5 -NT) and ecto-adenosine deaminase (E-ADA)] that metabolize nucleotides in platelets and lymphocytes and to evaluate cytokine levels of patients with HCV. Twenty five HCV patients and fifty healthy subjects (control group) were selected for this study. In lymphocytes, was observed a lower ATP hydrolysis (P<0.001), and higher ADP hydrolysis (P<0.001) and E- ADA activity (P<0.001) in HCV patients when compared to the control group. In addition, the results revealed a higher ATP and ADP hydrolysis by E-NTPDase and higher E-ADA activity in platelets of HCV patients when compared with the control group. Also, IL-4 and IL-10 were significantly (P<0.05) increased while IL-6 was decreased (P<0.05) in HCV patients. In conclusion, the results showed that the activities of E-NTPDase and E-ADA were altered in both lymphocytes and platelets of HCV patients. These alterations in enzymes activities may be possible regulatory mechanisms in an attempt to manage liver damage and ongoing inflammatory process. Furthermore, decreased IL-6 as well as increased IL-4 and IL-10 in HCV patients may suggest an antiinflammatory process and possible compensatory mechanisms in minimizing liver injury. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-12 2016-09-12 2016-07-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BASSO, Maria Emilha. METABOLISM OF NUCLEOTIDE AND NUCLEOSIDE ADENINE IN LYMPHOCYTES AND PLATELETS OF PATIENTS WITH HEPATITIS C. 2016. 69 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2016. http://repositorio.ufsm.br/handle/1/6039 |
identifier_str_mv |
BASSO, Maria Emilha. METABOLISM OF NUCLEOTIDE AND NUCLEOSIDE ADENINE IN LYMPHOCYTES AND PLATELETS OF PATIENTS WITH HEPATITIS C. 2016. 69 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2016. |
url |
http://repositorio.ufsm.br/handle/1/6039 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Análises Clínicas e Toxicológicas UFSM Programa de Pós-Graduação em Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Análises Clínicas e Toxicológicas UFSM Programa de Pós-Graduação em Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922130995445760 |