Envolvimento do receptor TRPA1 na nocicepção e neuroinflamação observada em modelos de esclerose múltipla em camundongos
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/25029 |
Resumo: | Multiple sclerosis (MS) is a demyelinating disease of the central nervous system (CNS), and neuropathic pain and headache are significant sensory alterations that are difficult to manage in these patients. In the different clinical forms of MS, pain and neuroinflammation are related to oxidative stress and the infiltration of inflammatory cells in the CNS. These oxidative stress products could activate ion channels, such as transient receptor potential ankyrin 1 (TRPA1). This study aimed to identify the role of the TRPA1 receptor in the nociception and neuroinflammation observed in experimental rodent models of MS. C57BL/6 female mice and mice with TRPA1 gene deletion were used (25-30 g). In both models, the antigen MOG35-55 (oligodendrocyte myelin glycoprotein) and the adjuvants Quil A and Complete Freund's Adjuvant (CFA) were administered for the relapsing-remitting MS (RRMS) and progressive MS (PMS) models, respectively. Nociception was measured by the von Frey test (mechanical allodynia in the paw and periorbital region) and the acetone test (cold allodynia in the paw region). The following treatments were performed: pregabalin, sumatriptan, olcegepant, TRPA1 antagonists (HC-030031 and A-967079), antioxidants (alfa-lipoic acid and apocynin), and TRPA1 antisense oligonucleotide by intragastrical or intrathecal route. The levels of endogenous TRPA1 agonists (hydrogen peroxide and 4-hydroxynonenal), and NADPH oxidase and superoxide dismutase (SOD) activity were evaluated in the spinal cord, trigeminal ganglion, and brainstem samples. Different markers of demyelination, neuroinflammation, and TRPA1 expression were also evaluated using the RNA expression technique for the EMRR mouse model. Immunohistochemistry was performed evaluating the Iba-1, GFAP, and OLIG-2 markers for the two MS models. Firstly, the induced mice to RRMS mouse model developed mechanical (hind paw and periorbital region) and cold (hind paw) allodynia. After, the administration of pregabalin, sumatriptan, olgepant, TRPA1 antagonists, antioxidants and the TRPA1 antisense oligonucleotide showed an antinociceptive effect in the EMRR mouse model. The levels of hydrogen peroxide and 4-hydroxynonenal and NADPH oxidase activity were increased in the spinal cord and trigeminal ganglion samples of RRMS induced mice. The deletion of TRPA1 channels attenuated the development of nociception in the EMRR and EMP induced mice. Genetic deletion of TRPA1 channels was also able to attenuate the increase in Iba-1 GFAP and OLIG-2 markers in the induced animals in both MS models. By Fastblue staining the TRPA1 deletion might prevent the demyelinating process. However, the TRPA1 knockout animals showed no attenuation in clinical scores after the two MS inductions. These results show the involvement of TRPA1 in nociception and neuroinflammation behaviors in these two MS models. |
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Envolvimento do receptor TRPA1 na nocicepção e neuroinflamação observada em modelos de esclerose múltipla em camundongosRole of TRPA1 in nociception and neuroinflamation caused by multiple sclerosis mouse modelsMOG35-55Estresse oxidativoHC-030031Peróxido de hidrogênio4-hidroxinonenalNADPH oxidaseOxidative stressHydrogen peroxide4-hydroxynonenalCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIAMultiple sclerosis (MS) is a demyelinating disease of the central nervous system (CNS), and neuropathic pain and headache are significant sensory alterations that are difficult to manage in these patients. In the different clinical forms of MS, pain and neuroinflammation are related to oxidative stress and the infiltration of inflammatory cells in the CNS. These oxidative stress products could activate ion channels, such as transient receptor potential ankyrin 1 (TRPA1). This study aimed to identify the role of the TRPA1 receptor in the nociception and neuroinflammation observed in experimental rodent models of MS. C57BL/6 female mice and mice with TRPA1 gene deletion were used (25-30 g). In both models, the antigen MOG35-55 (oligodendrocyte myelin glycoprotein) and the adjuvants Quil A and Complete Freund's Adjuvant (CFA) were administered for the relapsing-remitting MS (RRMS) and progressive MS (PMS) models, respectively. Nociception was measured by the von Frey test (mechanical allodynia in the paw and periorbital region) and the acetone test (cold allodynia in the paw region). The following treatments were performed: pregabalin, sumatriptan, olcegepant, TRPA1 antagonists (HC-030031 and A-967079), antioxidants (alfa-lipoic acid and apocynin), and TRPA1 antisense oligonucleotide by intragastrical or intrathecal route. The levels of endogenous TRPA1 agonists (hydrogen peroxide and 4-hydroxynonenal), and NADPH oxidase and superoxide dismutase (SOD) activity were evaluated in the spinal cord, trigeminal ganglion, and brainstem samples. Different markers of demyelination, neuroinflammation, and TRPA1 expression were also evaluated using the RNA expression technique for the EMRR mouse model. Immunohistochemistry was performed evaluating the Iba-1, GFAP, and OLIG-2 markers for the two MS models. Firstly, the induced mice to RRMS mouse model developed mechanical (hind paw and periorbital region) and cold (hind paw) allodynia. After, the administration of pregabalin, sumatriptan, olgepant, TRPA1 antagonists, antioxidants and the TRPA1 antisense oligonucleotide showed an antinociceptive effect in the EMRR mouse model. The levels of hydrogen peroxide and 4-hydroxynonenal and NADPH oxidase activity were increased in the spinal cord and trigeminal ganglion samples of RRMS induced mice. The deletion of TRPA1 channels attenuated the development of nociception in the EMRR and EMP induced mice. Genetic deletion of TRPA1 channels was also able to attenuate the increase in Iba-1 GFAP and OLIG-2 markers in the induced animals in both MS models. By Fastblue staining the TRPA1 deletion might prevent the demyelinating process. However, the TRPA1 knockout animals showed no attenuation in clinical scores after the two MS inductions. These results show the involvement of TRPA1 in nociception and neuroinflammation behaviors in these two MS models.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESA esclerose múltipla (EM) é uma doença desmielinizante do sistema nervoso central (SNC), sendo a dor neuropática e a dor de cabeça alterações sensoriais relevantes e de difícil manejo nos pacientes. A dor e a neuroinflamação nas diferentes formas clínicas da EM estão relacionadas com o estresse oxidativo e a infiltração de células inflamatórias no SNC. Estes produtos do estresse oxidativo poderiam ativar canais iônicos, como os receptores de potencial transitório anquirina 1 (TRPA1). O objetivo deste estudo foi identificar o papel do receptor TRPA1 na nocicepção e neuroinflamação observadas em modelos experimentais de EM. Para isso foram utilizados camundongos fêmeas C57BL/6 e camundongos com deleção gênica para o TRPA1 (25-30 g). Em ambos os modelos foram administrados o antígeno MOG35-55 (glicoproteína da mielina de oligodendrócito), e os adjuvantes Quil A e Completo de Freund (ACF) para os modelos de EM recorrente-remitente (EMRR) e EM progressiva (EMP), respectivamente. A nocicepção foi mensurada pelo teste de von Frey (alodinia mecânica, na região da pata e periorbital) e o teste de acetona (alodinia ao frio, na região da pata). Foram realizados os seguintes tratamentos: pregabalina, sumatriptana, olcegepant, antagonistas do TRPA1 (HC-030031 e A-967079), antioxidantes (ácido alfa-lipoico e apocinina) e oligonucleotídeo antissentido para o TRPA1 por via oral ou intratecal. Foram avaliados os níveis de agonistas endógenos do TRPA1 (peróxido de hidrogênio e 4-hidroxinonenal) e a atividade da NADPH oxidase e superóxido dismutase (SOD) na medula espinal, gânglio trigeminal e tronco encefálico. Diferentes marcadores de desmielinização, neuroinflamação e a expressão do TRPA1 foram também avaliados através da técnica de expressão gênica para o modelo de EMRR. Por imunoistoquímica foram avaliados os marcadores Iba-1, GFAP e OLIG-2 para os dois modelos. Como resultados, no modelo de EMRR, observou-se a presença de alodinia mecânica (pata traseira e região periorbital) e ao frio (pata traseira). A administração de pregabalina, sumatriptana, olgepant, antagonistas do TRPA1, antioxidantes e o oligonucleotideo antisentido do TRPA1 apresentaram efeito antinociceptivo no modelo EMRR. Foi observado o aumento dos níveis de peróxido de hidrogênio e 4-hidroxinonenal, e a atividade da NADPH oxidase na medula espinal e gânglio trigeminal. Houve uma atenuação da nocicepção nos animais com deleção dos canais TRPA1 induzidos aos modelos de EMRR e EMP. A deleção dos canais TRPA1 também foi capaz de atenuar o aumento dos marcadores Iba-1 e GFAP, bem como o OLIG-2 em animais induzidos aos dois modelos. Pela coloração fast blue observou-se uma prevenção nos animais nocautes para o TRPA1. Porém os animais nocautes para o TRPA1 não apresentaram atenuação nos escores clínicos após as duas induções. Portanto, esses resultados mostram o envolvimento do TRPA1 nos comportamentos de nocicepção e neuroinflamação nos dois modelos de EM.Universidade Federal de Santa MariaBrasilFarmacologiaUFSMPrograma de Pós-Graduação em FarmacologiaCentro de Ciências da SaúdeSantos, Gabriela Trevisan doshttp://lattes.cnpq.br/7186082133291911Fernandes, Elizabeth SoaresWerner, Maria Fernanda de PaulaOliveira, Mauro SchneiderNascimento, Patrícia Severo doDalenogare, Diéssica Padilha2022-06-23T14:11:14Z2022-06-23T14:11:14Z2022-05-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/25029porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-06-23T14:11:14Zoai:repositorio.ufsm.br:1/25029Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-06-23T14:11:14Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Envolvimento do receptor TRPA1 na nocicepção e neuroinflamação observada em modelos de esclerose múltipla em camundongos Role of TRPA1 in nociception and neuroinflamation caused by multiple sclerosis mouse models |
title |
Envolvimento do receptor TRPA1 na nocicepção e neuroinflamação observada em modelos de esclerose múltipla em camundongos |
spellingShingle |
Envolvimento do receptor TRPA1 na nocicepção e neuroinflamação observada em modelos de esclerose múltipla em camundongos Dalenogare, Diéssica Padilha MOG35-55 Estresse oxidativo HC-030031 Peróxido de hidrogênio 4-hidroxinonenal NADPH oxidase Oxidative stress Hydrogen peroxide 4-hydroxynonenal CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
title_short |
Envolvimento do receptor TRPA1 na nocicepção e neuroinflamação observada em modelos de esclerose múltipla em camundongos |
title_full |
Envolvimento do receptor TRPA1 na nocicepção e neuroinflamação observada em modelos de esclerose múltipla em camundongos |
title_fullStr |
Envolvimento do receptor TRPA1 na nocicepção e neuroinflamação observada em modelos de esclerose múltipla em camundongos |
title_full_unstemmed |
Envolvimento do receptor TRPA1 na nocicepção e neuroinflamação observada em modelos de esclerose múltipla em camundongos |
title_sort |
Envolvimento do receptor TRPA1 na nocicepção e neuroinflamação observada em modelos de esclerose múltipla em camundongos |
author |
Dalenogare, Diéssica Padilha |
author_facet |
Dalenogare, Diéssica Padilha |
author_role |
author |
dc.contributor.none.fl_str_mv |
Santos, Gabriela Trevisan dos http://lattes.cnpq.br/7186082133291911 Fernandes, Elizabeth Soares Werner, Maria Fernanda de Paula Oliveira, Mauro Schneider Nascimento, Patrícia Severo do |
dc.contributor.author.fl_str_mv |
Dalenogare, Diéssica Padilha |
dc.subject.por.fl_str_mv |
MOG35-55 Estresse oxidativo HC-030031 Peróxido de hidrogênio 4-hidroxinonenal NADPH oxidase Oxidative stress Hydrogen peroxide 4-hydroxynonenal CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
topic |
MOG35-55 Estresse oxidativo HC-030031 Peróxido de hidrogênio 4-hidroxinonenal NADPH oxidase Oxidative stress Hydrogen peroxide 4-hydroxynonenal CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
description |
Multiple sclerosis (MS) is a demyelinating disease of the central nervous system (CNS), and neuropathic pain and headache are significant sensory alterations that are difficult to manage in these patients. In the different clinical forms of MS, pain and neuroinflammation are related to oxidative stress and the infiltration of inflammatory cells in the CNS. These oxidative stress products could activate ion channels, such as transient receptor potential ankyrin 1 (TRPA1). This study aimed to identify the role of the TRPA1 receptor in the nociception and neuroinflammation observed in experimental rodent models of MS. C57BL/6 female mice and mice with TRPA1 gene deletion were used (25-30 g). In both models, the antigen MOG35-55 (oligodendrocyte myelin glycoprotein) and the adjuvants Quil A and Complete Freund's Adjuvant (CFA) were administered for the relapsing-remitting MS (RRMS) and progressive MS (PMS) models, respectively. Nociception was measured by the von Frey test (mechanical allodynia in the paw and periorbital region) and the acetone test (cold allodynia in the paw region). The following treatments were performed: pregabalin, sumatriptan, olcegepant, TRPA1 antagonists (HC-030031 and A-967079), antioxidants (alfa-lipoic acid and apocynin), and TRPA1 antisense oligonucleotide by intragastrical or intrathecal route. The levels of endogenous TRPA1 agonists (hydrogen peroxide and 4-hydroxynonenal), and NADPH oxidase and superoxide dismutase (SOD) activity were evaluated in the spinal cord, trigeminal ganglion, and brainstem samples. Different markers of demyelination, neuroinflammation, and TRPA1 expression were also evaluated using the RNA expression technique for the EMRR mouse model. Immunohistochemistry was performed evaluating the Iba-1, GFAP, and OLIG-2 markers for the two MS models. Firstly, the induced mice to RRMS mouse model developed mechanical (hind paw and periorbital region) and cold (hind paw) allodynia. After, the administration of pregabalin, sumatriptan, olgepant, TRPA1 antagonists, antioxidants and the TRPA1 antisense oligonucleotide showed an antinociceptive effect in the EMRR mouse model. The levels of hydrogen peroxide and 4-hydroxynonenal and NADPH oxidase activity were increased in the spinal cord and trigeminal ganglion samples of RRMS induced mice. The deletion of TRPA1 channels attenuated the development of nociception in the EMRR and EMP induced mice. Genetic deletion of TRPA1 channels was also able to attenuate the increase in Iba-1 GFAP and OLIG-2 markers in the induced animals in both MS models. By Fastblue staining the TRPA1 deletion might prevent the demyelinating process. However, the TRPA1 knockout animals showed no attenuation in clinical scores after the two MS inductions. These results show the involvement of TRPA1 in nociception and neuroinflammation behaviors in these two MS models. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-23T14:11:14Z 2022-06-23T14:11:14Z 2022-05-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/25029 |
url |
http://repositorio.ufsm.br/handle/1/25029 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Farmacologia UFSM Programa de Pós-Graduação em Farmacologia Centro de Ciências da Saúde |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Farmacologia UFSM Programa de Pós-Graduação em Farmacologia Centro de Ciências da Saúde |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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1805922078362173440 |