Rizogênese in vitro e aclimatização de Luehea divaricata Mart. & Zucc.
Main Author: | |
---|---|
Publication Date: | 2016 |
Format: | Master thesis |
Language: | por |
Source: | Manancial - Repositório Digital da UFSM |
dARK ID: | ark:/26339/001300000gwhc |
Download full: | http://repositorio.ufsm.br/handle/1/8777 |
Summary: | In front of the need to recover the original forest cover of the Atlantic Forest, it is necessary to adopt restoration programs of deforested areas using native species. Luehea divaricata Martius et Zuccarini, popularly known as açoita-cavalo, is a native tree specie of the Atlantic Forest biome widely used for the recovery of areas. It is a forest species that has its wood used for many different purposes, which contributed to the reduction of their natural populations, causing difficulties to find viable seeds of the species. Given this fact, the micropropagation becomes a viable alternative for the production of high quality seedlings, providing thus the formation of healthy populations for use in restoration projects of degraded areas. This way, this study aimed to optimize the rooting in vitro process and acclimatization of the species, and, for this, different concentrations of salts of the WPM nutritive medium for in vitro root formation shoots of Luehea divaricata were initially evaluated. Then, the effects of the addition indolebutyric acid (IBA) as a treatment "pulse" and naphthaleneacetic acid (NAA) in the nutritive medium were studied for rooting in vitro specie. The effect of the combination of vermiculite volume with the nutritive medium WPM/2 in root formation in vitro of Luehea divaricata was also analyzed in this study, as well as the implication of sucrose and different concentrations of substrates in the species acclimatization. The nutritive medium WPM/2 showed the most promising results for the development of Luehea divaricata, reaching values of 44,52% in the formation of primary roots after 45 days of in vitro culture. Treatment "pulse" with different concentrations of IBA and the use of NAA concentrations are not efficient for in vitro root formation in Luehea divaricata. The combinations of 20mL of nutritive medium + 15cm³ vermiculite, 20mL of nutritive medium + 30cm³ of vermiculite and 30mL of nutritive medium + 30cm³ of vermiculite were more promising for in vitro root formation in the species, providing averages of 39,61% in the rooting after 45 days of cultivation. Finally, for acclimatization in vitro and ex vitro substrates based on bio-stabilized pinus bark or based on turf, calcareous calcitic and composted pinus bark may be used, independently of sucrose concentrations tested during in vitro cultivation. |
id |
UFSM_19a000f5659a9a7b9cc3bc8cd19c82cc |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/8777 |
network_acronym_str |
UFSM |
network_name_str |
Manancial - Repositório Digital da UFSM |
repository_id_str |
|
spelling |
Rizogênese in vitro e aclimatização de Luehea divaricata Mart. & Zucc.Rhizogenesis in vitro and acclimatization of Luehea divaricata Mart. & Zucc.Açoita-cavaloMicropropagaçãoAuxinaSubstratos alternativosAçoita-cavaloMicropropagationAuxinAlternative substratesCNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTALIn front of the need to recover the original forest cover of the Atlantic Forest, it is necessary to adopt restoration programs of deforested areas using native species. Luehea divaricata Martius et Zuccarini, popularly known as açoita-cavalo, is a native tree specie of the Atlantic Forest biome widely used for the recovery of areas. It is a forest species that has its wood used for many different purposes, which contributed to the reduction of their natural populations, causing difficulties to find viable seeds of the species. Given this fact, the micropropagation becomes a viable alternative for the production of high quality seedlings, providing thus the formation of healthy populations for use in restoration projects of degraded areas. This way, this study aimed to optimize the rooting in vitro process and acclimatization of the species, and, for this, different concentrations of salts of the WPM nutritive medium for in vitro root formation shoots of Luehea divaricata were initially evaluated. Then, the effects of the addition indolebutyric acid (IBA) as a treatment "pulse" and naphthaleneacetic acid (NAA) in the nutritive medium were studied for rooting in vitro specie. The effect of the combination of vermiculite volume with the nutritive medium WPM/2 in root formation in vitro of Luehea divaricata was also analyzed in this study, as well as the implication of sucrose and different concentrations of substrates in the species acclimatization. The nutritive medium WPM/2 showed the most promising results for the development of Luehea divaricata, reaching values of 44,52% in the formation of primary roots after 45 days of in vitro culture. Treatment "pulse" with different concentrations of IBA and the use of NAA concentrations are not efficient for in vitro root formation in Luehea divaricata. The combinations of 20mL of nutritive medium + 15cm³ vermiculite, 20mL of nutritive medium + 30cm³ of vermiculite and 30mL of nutritive medium + 30cm³ of vermiculite were more promising for in vitro root formation in the species, providing averages of 39,61% in the rooting after 45 days of cultivation. Finally, for acclimatization in vitro and ex vitro substrates based on bio-stabilized pinus bark or based on turf, calcareous calcitic and composted pinus bark may be used, independently of sucrose concentrations tested during in vitro cultivation.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorDiante da necessidade de recuperação da cobertura florestal original do bioma Mata Atlântica, é imprescindível a adoção de programas de restauração de áreas desmatadas utilizando espécies florestais nativas. Luehea divaricata Martius et Zuccarini, conhecida popularmente como açoita-cavalo, é uma espécie florestal nativa do bioma Mata Atlântica muito utilizada para a recuperação de áreas. Trata-se de uma espécie florestal que tem sua madeira empregada para as mais diversas finalidades, fato que contribuiu para a redução de suas populações naturais, ocasionando dificuldades para encontrar sementes viáveis da espécie. Diante desse fato, a micropropagação torna-se uma alternativa viável para a produção de mudas de alta qualidade, propiciando, assim, a formação de populações sadias para o uso em projetos de recuperação de áreas degradadas. Face ao exposto, o presente trabalho teve como objetivo otimizar os processos de enraizamento in vitro e aclimatização da espécie, e, para isso, foram avaliadas, inicialmente, diferentes concentrações dos sais do meio nutritivo WPM na rizogênese in vitro de brotações de Luehea divaricata. Em seguida, foram estudados os efeitos da adição, ao meio nutritivo, do Ácido Indolbutírico (AIB) como tratamento pulse e do Ácido Naftalenoacético (ANA) para o enraizamento in vitro da espécie. O efeito da combinação do volume de vermiculita com o meio nutritivo WPM/2 na rizogênese in vitro de Luehea divaricata também foi analisado, bem como a implicação de concentrações de sacarose e diferentes substratos na aclimatização da espécie. Os resultados obtidos indicam que o meio nutritivo WPM/2 apresentou resultados mais promissores para o desenvolvimento de Luehea divaricata, alcançando valores de 44,52% na formação de raízes primárias aos 45 dias de cultivo in vitro. O tratamento pulse com diferentes concentrações de AIB e o emprego de concentrações de ANA não são eficientes para a rizogênese in vitro de Luehea divaricata. As combinações de 20mL de meio nutritivo + 15cm³ de vermiculita, 20mL de meio nutritivo + 30cm³ de vermiculita e 30mL de meio nutritivo + 30cm³ de vermiculita são mais promissoras na rizogênese in vitro da espécie, proporcionando médias de 39,61% no enraizamento aos 45 dias de cultivo. Por fim, para a fase de aclimatização in vitro e ex vitro podem ser utilizados os substratos à base de casca de pinus bio-estabilizada e à base de turfa, calcário calcítico e casca de pinus compostada, independentemente das concentrações de sacarose testadas durante o cultivo in vitro.Universidade Federal de Santa MariaBRRecursos Florestais e Engenharia FlorestalUFSMPrograma de Pós-Graduação em Engenharia FlorestalReiniger, Lia Rejane Silveirahttp://lattes.cnpq.br/5739294882585391Heinzmann, Berta Mariahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784229Z7León, Enrique Asterio Benítezhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4207719A6Silva, Karol Buuron da2016-07-182016-07-182016-02-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfSILVA, Karol Buuron da. RHIZOGENESIS IN VITRO AND ACCLIMATIZATION OF Luehea divaricata Mart. & Zucc.. 2016. 84 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2016.http://repositorio.ufsm.br/handle/1/8777ark:/26339/001300000gwhcporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2021-09-23T17:21:31Zoai:repositorio.ufsm.br:1/8777Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2024-07-29T10:39:27.901316Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Rizogênese in vitro e aclimatização de Luehea divaricata Mart. & Zucc. Rhizogenesis in vitro and acclimatization of Luehea divaricata Mart. & Zucc. |
title |
Rizogênese in vitro e aclimatização de Luehea divaricata Mart. & Zucc. |
spellingShingle |
Rizogênese in vitro e aclimatização de Luehea divaricata Mart. & Zucc. Silva, Karol Buuron da Açoita-cavalo Micropropagação Auxina Substratos alternativos Açoita-cavalo Micropropagation Auxin Alternative substrates CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
title_short |
Rizogênese in vitro e aclimatização de Luehea divaricata Mart. & Zucc. |
title_full |
Rizogênese in vitro e aclimatização de Luehea divaricata Mart. & Zucc. |
title_fullStr |
Rizogênese in vitro e aclimatização de Luehea divaricata Mart. & Zucc. |
title_full_unstemmed |
Rizogênese in vitro e aclimatização de Luehea divaricata Mart. & Zucc. |
title_sort |
Rizogênese in vitro e aclimatização de Luehea divaricata Mart. & Zucc. |
author |
Silva, Karol Buuron da |
author_facet |
Silva, Karol Buuron da |
author_role |
author |
dc.contributor.none.fl_str_mv |
Reiniger, Lia Rejane Silveira http://lattes.cnpq.br/5739294882585391 Heinzmann, Berta Maria http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784229Z7 León, Enrique Asterio Benítez http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4207719A6 |
dc.contributor.author.fl_str_mv |
Silva, Karol Buuron da |
dc.subject.por.fl_str_mv |
Açoita-cavalo Micropropagação Auxina Substratos alternativos Açoita-cavalo Micropropagation Auxin Alternative substrates CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
topic |
Açoita-cavalo Micropropagação Auxina Substratos alternativos Açoita-cavalo Micropropagation Auxin Alternative substrates CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
description |
In front of the need to recover the original forest cover of the Atlantic Forest, it is necessary to adopt restoration programs of deforested areas using native species. Luehea divaricata Martius et Zuccarini, popularly known as açoita-cavalo, is a native tree specie of the Atlantic Forest biome widely used for the recovery of areas. It is a forest species that has its wood used for many different purposes, which contributed to the reduction of their natural populations, causing difficulties to find viable seeds of the species. Given this fact, the micropropagation becomes a viable alternative for the production of high quality seedlings, providing thus the formation of healthy populations for use in restoration projects of degraded areas. This way, this study aimed to optimize the rooting in vitro process and acclimatization of the species, and, for this, different concentrations of salts of the WPM nutritive medium for in vitro root formation shoots of Luehea divaricata were initially evaluated. Then, the effects of the addition indolebutyric acid (IBA) as a treatment "pulse" and naphthaleneacetic acid (NAA) in the nutritive medium were studied for rooting in vitro specie. The effect of the combination of vermiculite volume with the nutritive medium WPM/2 in root formation in vitro of Luehea divaricata was also analyzed in this study, as well as the implication of sucrose and different concentrations of substrates in the species acclimatization. The nutritive medium WPM/2 showed the most promising results for the development of Luehea divaricata, reaching values of 44,52% in the formation of primary roots after 45 days of in vitro culture. Treatment "pulse" with different concentrations of IBA and the use of NAA concentrations are not efficient for in vitro root formation in Luehea divaricata. The combinations of 20mL of nutritive medium + 15cm³ vermiculite, 20mL of nutritive medium + 30cm³ of vermiculite and 30mL of nutritive medium + 30cm³ of vermiculite were more promising for in vitro root formation in the species, providing averages of 39,61% in the rooting after 45 days of cultivation. Finally, for acclimatization in vitro and ex vitro substrates based on bio-stabilized pinus bark or based on turf, calcareous calcitic and composted pinus bark may be used, independently of sucrose concentrations tested during in vitro cultivation. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-18 2016-07-18 2016-02-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SILVA, Karol Buuron da. RHIZOGENESIS IN VITRO AND ACCLIMATIZATION OF Luehea divaricata Mart. & Zucc.. 2016. 84 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2016. http://repositorio.ufsm.br/handle/1/8777 |
dc.identifier.dark.fl_str_mv |
ark:/26339/001300000gwhc |
identifier_str_mv |
SILVA, Karol Buuron da. RHIZOGENESIS IN VITRO AND ACCLIMATIZATION OF Luehea divaricata Mart. & Zucc.. 2016. 84 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2016. ark:/26339/001300000gwhc |
url |
http://repositorio.ufsm.br/handle/1/8777 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Recursos Florestais e Engenharia Florestal UFSM Programa de Pós-Graduação em Engenharia Florestal |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Recursos Florestais e Engenharia Florestal UFSM Programa de Pós-Graduação em Engenharia Florestal |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1814439790252130304 |