Production of biosurfactants by Mucoralean fungi isolated from Caatinga bioma soil using industrial waste as renewable substrates
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/25332 |
Resumo: | In this work it was investigated the potential of Mucorales fungi isolated from the Caatinga of Pernambuco state for production of biosurfactants using renewable substrates. The strains (Mucor circinelloides UCP 0005, M. circinelloides UCP 0006 and Rhizopus arrhizus UCP 1609) were cultivated in alternative culture media consisting of instant noodle waste (INW), corn steep liquor (CSL) and post-frying soybean oil (PFSO), according to conditions established by a 23 full-factorial design (FFD). The production of biosurfactants was evaluated by determining surface tension and emulsification index (EI24) and statistical analysis was performed using Pareto diagram. The presence of the main sources of carbon and nitrogen in production medium was confirmed by FTIR spectroscopy. According to the results, the three fungi evaluated were able of produce biosurfactant in media containing renewable sources. However, the strain that showed the greatest reduction in surface tension (72 to 27 mN/m) was M. circinelloides UCP 0006 in condition 3 of the FFD (1% INW and 4% CSL, in absence of PFSO). The infrared analysis of the INW showed the presence of carbohydrates, fatty acids and proteins, proving that this is a suitable substrate for the cultivation of fungi. The biosurfactants produced by M. circinelloides UCP 0005 and M. circinelloides UCP 0006 were able to form water-in-oil emulsions and the biosurfactant from R. arrhizus UCP 1609 formed oil-in-water emulsions. The present study demonstrated that the three Mucorales fungi tested were able to produce biosurfactants from renewable sources, with emphasis on M. circinelloides UCP 0006. |
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Production of biosurfactants by Mucoralean fungi isolated from Caatinga bioma soil using industrial waste as renewable substrates Producción de biosurfactantes por hongos Mucorales aislados del suelo del bioma Caatinga utilizando residuos industriales como sustratos renovables Produção de biossurfactantes por fungos Mucorales isolados do solo do bioma Caatinga usando resíduos industriais como substratos renováveisBiomoléculaHongosTensión superficialEmulsificaciónSostenibilidad.BiomoleculeFungiSurface tensionEmulsificationSustainability.BiomoléculaFungosTensão superficialEmulsificaçãoSustentabilidade.In this work it was investigated the potential of Mucorales fungi isolated from the Caatinga of Pernambuco state for production of biosurfactants using renewable substrates. The strains (Mucor circinelloides UCP 0005, M. circinelloides UCP 0006 and Rhizopus arrhizus UCP 1609) were cultivated in alternative culture media consisting of instant noodle waste (INW), corn steep liquor (CSL) and post-frying soybean oil (PFSO), according to conditions established by a 23 full-factorial design (FFD). The production of biosurfactants was evaluated by determining surface tension and emulsification index (EI24) and statistical analysis was performed using Pareto diagram. The presence of the main sources of carbon and nitrogen in production medium was confirmed by FTIR spectroscopy. According to the results, the three fungi evaluated were able of produce biosurfactant in media containing renewable sources. However, the strain that showed the greatest reduction in surface tension (72 to 27 mN/m) was M. circinelloides UCP 0006 in condition 3 of the FFD (1% INW and 4% CSL, in absence of PFSO). The infrared analysis of the INW showed the presence of carbohydrates, fatty acids and proteins, proving that this is a suitable substrate for the cultivation of fungi. The biosurfactants produced by M. circinelloides UCP 0005 and M. circinelloides UCP 0006 were able to form water-in-oil emulsions and the biosurfactant from R. arrhizus UCP 1609 formed oil-in-water emulsions. The present study demonstrated that the three Mucorales fungi tested were able to produce biosurfactants from renewable sources, with emphasis on M. circinelloides UCP 0006.En este trabajo se investigó el potencial de hongos Mucorales aislados de la Caatinga do estado de Pernambuco para la producción de biosurfactantes utilizando sustratos renovables. Las cepas (Mucor circinelloides UCP 0005, M. circinelloides UCP 0006 y Rhizopus arrhizus UCP 1609) se cultivaron en medios de cultivo compuestos por residuo de fideos instantáneos, agua residual de maíz y aceite de soya post fritura, de acuerdo con un diseño factorial completo 23. La producción de biosurfactante se evaluó por la determinación de la tensión superficial y el índice de emulsificación (IE24) y el análisis estadístico se realizó mediante el diagrama de Pareto. La presencia de las principales fuentes de carbono y nitrógeno en el medio de producción fue confirmada por espectroscopía FTIR. De acuerdo con los resultados, los tres hongos evaluados fueron capaces de producir biosurfactantes en medios que contenían fuentes renovables. Sin embargo, la cepa que mostró la mayor reducción de la tensión superficial (72 a 27 mN/m) fue M. circinelloides UCP 0006 en la condición 3 del diseño factorial (1% residuo de fideos instantáneos, 4% agua residual de maíz, en ausencia de aceite de soya post fritura). El análisis infrarrojo del residuo de fideos instantáneos mostró la presencia de carbohidratos, ácidos grasos y proteínas, comprobando que este es un sustrato adecuado para el cultivo de hongos. Los biosurfactantes producidos por M. circinelloides UCP 0005 y M. circinelloides UCP 0006 formaron emulsiones de tipo agua en aceite y el biossurfactante de R. arrhizus UCP 1609 formó emulsiones de tipo aceite en agua. El presente estudio demostró que los tres hongos Mucorales evaluados produjeron biosurfactantes a partir de fuentes renovables, destacándose M. circinelloides UCP 0006.Neste trabalho foi investigado o potencial de fungos Mucorales isolados da Caatinga do estado de Pernambuco para produção de biossurfactantes utilizando substratos renováveis. As cepas (Mucor circinelloides UCP 0005, M. circinelloides UCP 0006 e Rhizopus arrhizus UCP 1609) foram cultivadas em meios de cultivo alternativos constituídos por resíduo de macarrão instantâneo, milhocina e óleo de soja pós-fritura, de acordo com um planejamento fatorial completo 23. A produção de biossurfactantes foi avaliada pela determinação da tensão superficial e índice de emulsificação (IE24) e a análise estatística foi realizada por meio do diagrama de Pareto. A presença das principais fontes de carbono e nitrogênio no meio de produção foi confirmada por espectroscopia FTIR. De acordo com os resultados, os três fungos avaliados foram capazes de produzir biossurfactante em meios contendo as fontes renováveis. No entanto, a cepa que apresentou maior redução na tensão superficial (72 a 27 mN/m) foi M. circinelloides UCP 0006 na condição 3 do planejamento (1% resíduo de macarrão instantâneo, 4% de milhocina, na ausência do óleo de soja pós-fritura). A análise no infravermelho do resíduo de macarrão instantâneo mostrou a presença de carboidratos, ácidos graxos e proteínas, comprovando que este é um substrato adequado para o cultivo de fungos. Os biossurfactantes produzidos por M. circinelloides UCP 0005 e M. circinelloides UCP 0006 foram capazes de formar emulsões do tipo água em óleo e o biossurfactante de R. arrhizus UCP 1609 formou emulsões do tipo óleo em água. O presente estudo demonstrou que os três fungos Mucorales testados foram capazes de produzir biossurfactantes a partir de fontes renováveis, com destaque para M. circinelloides UCP 0006.Research, Society and Development2022-01-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2533210.33448/rsd-v11i2.25332Research, Society and Development; Vol. 11 No. 2; e13411225332Research, Society and Development; Vol. 11 Núm. 2; e13411225332Research, Society and Development; v. 11 n. 2; e134112253322525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/25332/22387Copyright (c) 2022 Thayná Rhomana da Silva Cândido; Rafael de Souza Mendonça ; Uiara Maria de Barros Lira Lins; Adriana Ferreira de Souza; Dayana Montero Rodriguez; Galba Maria de Campos-Takaki; Rosileide Fontenele da Silva Andradehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCândido, Thayná Rhomana da Silva Mendonça , Rafael de SouzaLins, Uiara Maria de Barros Lira Souza, Adriana Ferreira de Montero Rodriguez, DayanaCampos-Takaki, Galba Maria de Andrade, Rosileide Fontenele da Silva 2022-02-07T01:42:50Zoai:ojs.pkp.sfu.ca:article/25332Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:43:38.596673Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Production of biosurfactants by Mucoralean fungi isolated from Caatinga bioma soil using industrial waste as renewable substrates Producción de biosurfactantes por hongos Mucorales aislados del suelo del bioma Caatinga utilizando residuos industriales como sustratos renovables Produção de biossurfactantes por fungos Mucorales isolados do solo do bioma Caatinga usando resíduos industriais como substratos renováveis |
title |
Production of biosurfactants by Mucoralean fungi isolated from Caatinga bioma soil using industrial waste as renewable substrates |
spellingShingle |
Production of biosurfactants by Mucoralean fungi isolated from Caatinga bioma soil using industrial waste as renewable substrates Cândido, Thayná Rhomana da Silva Biomolécula Hongos Tensión superficial Emulsificación Sostenibilidad. Biomolecule Fungi Surface tension Emulsification Sustainability. Biomolécula Fungos Tensão superficial Emulsificação Sustentabilidade. |
title_short |
Production of biosurfactants by Mucoralean fungi isolated from Caatinga bioma soil using industrial waste as renewable substrates |
title_full |
Production of biosurfactants by Mucoralean fungi isolated from Caatinga bioma soil using industrial waste as renewable substrates |
title_fullStr |
Production of biosurfactants by Mucoralean fungi isolated from Caatinga bioma soil using industrial waste as renewable substrates |
title_full_unstemmed |
Production of biosurfactants by Mucoralean fungi isolated from Caatinga bioma soil using industrial waste as renewable substrates |
title_sort |
Production of biosurfactants by Mucoralean fungi isolated from Caatinga bioma soil using industrial waste as renewable substrates |
author |
Cândido, Thayná Rhomana da Silva |
author_facet |
Cândido, Thayná Rhomana da Silva Mendonça , Rafael de Souza Lins, Uiara Maria de Barros Lira Souza, Adriana Ferreira de Montero Rodriguez, Dayana Campos-Takaki, Galba Maria de Andrade, Rosileide Fontenele da Silva |
author_role |
author |
author2 |
Mendonça , Rafael de Souza Lins, Uiara Maria de Barros Lira Souza, Adriana Ferreira de Montero Rodriguez, Dayana Campos-Takaki, Galba Maria de Andrade, Rosileide Fontenele da Silva |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Cândido, Thayná Rhomana da Silva Mendonça , Rafael de Souza Lins, Uiara Maria de Barros Lira Souza, Adriana Ferreira de Montero Rodriguez, Dayana Campos-Takaki, Galba Maria de Andrade, Rosileide Fontenele da Silva |
dc.subject.por.fl_str_mv |
Biomolécula Hongos Tensión superficial Emulsificación Sostenibilidad. Biomolecule Fungi Surface tension Emulsification Sustainability. Biomolécula Fungos Tensão superficial Emulsificação Sustentabilidade. |
topic |
Biomolécula Hongos Tensión superficial Emulsificación Sostenibilidad. Biomolecule Fungi Surface tension Emulsification Sustainability. Biomolécula Fungos Tensão superficial Emulsificação Sustentabilidade. |
description |
In this work it was investigated the potential of Mucorales fungi isolated from the Caatinga of Pernambuco state for production of biosurfactants using renewable substrates. The strains (Mucor circinelloides UCP 0005, M. circinelloides UCP 0006 and Rhizopus arrhizus UCP 1609) were cultivated in alternative culture media consisting of instant noodle waste (INW), corn steep liquor (CSL) and post-frying soybean oil (PFSO), according to conditions established by a 23 full-factorial design (FFD). The production of biosurfactants was evaluated by determining surface tension and emulsification index (EI24) and statistical analysis was performed using Pareto diagram. The presence of the main sources of carbon and nitrogen in production medium was confirmed by FTIR spectroscopy. According to the results, the three fungi evaluated were able of produce biosurfactant in media containing renewable sources. However, the strain that showed the greatest reduction in surface tension (72 to 27 mN/m) was M. circinelloides UCP 0006 in condition 3 of the FFD (1% INW and 4% CSL, in absence of PFSO). The infrared analysis of the INW showed the presence of carbohydrates, fatty acids and proteins, proving that this is a suitable substrate for the cultivation of fungi. The biosurfactants produced by M. circinelloides UCP 0005 and M. circinelloides UCP 0006 were able to form water-in-oil emulsions and the biosurfactant from R. arrhizus UCP 1609 formed oil-in-water emulsions. The present study demonstrated that the three Mucorales fungi tested were able to produce biosurfactants from renewable sources, with emphasis on M. circinelloides UCP 0006. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/25332 10.33448/rsd-v11i2.25332 |
url |
https://rsdjournal.org/index.php/rsd/article/view/25332 |
identifier_str_mv |
10.33448/rsd-v11i2.25332 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/25332/22387 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 2; e13411225332 Research, Society and Development; Vol. 11 Núm. 2; e13411225332 Research, Society and Development; v. 11 n. 2; e13411225332 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052820215562240 |