Phytochemical Profile, and Antiproliferative and Proapoptotic Effects ofPouteria ramiflora(Mart.) Radlk. Leaf Extract, and Its Synergism with Cisplatin in HepG2 Cells

Detalhes bibliográficos
Autor(a) principal: Tuttis, Katiuska
Data de Publicação: 2020
Outros Autores: Costa, Daryne Lu Maldonado Gomes da [UNESP], Serpeloni, Juliana Mara, Santos, Lourdes Campaner dos [UNESP], Varanda, Eliana Aparecida [UNESP], Vilegas, Wagner [UNESP], Martinez-Lopez, Wilner, Colus, Ilce Mara de Syllos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1089/jmf.2020.0045
http://hdl.handle.net/11449/197164
Resumo: Different species of the genusPouteriahave been used in folk medicine for the treatment of inflammation, fever, ulcers, diabetes, and diarrhea. We analyzed the phytochemical profile of the hydroethanolic extract fromPouteria ramifloraleaves by electrospray ionization ion trap tandem mass spectrometry and high-performance liquid chromatography-diode array detection, and examined whether it alone and in combination with cisplatin interfered with cell proliferation and death processes in HepG2 (human hepatocellular carcinoma) and FGH (human gingival fibroblasts) cells. Five compounds were identified in the extract: gallic acid, myricetin-3-O-alpha-l-arabinopyranoside, quercetin-3-O-beta-d-galactopyranoside, myricetin-3-O-alpha-l-rhamnopyranoside, and myricetin-3-O-beta-d-galactopyranoside. The extract was cytotoxic to both cell lines by inducing apoptotic cell death and acted in synergy with cisplatin; such effect was stronger in HepG2 cells than in FGH cells, demonstrating some selectivity to tumor cells. In HepG2 cells, the extract exerted antiproliferative effect mediated by induction of cell cycle arrest at the S and G2/M phases. Association of the extract with cisplatin enhanced the latter's antiproliferative effect, arrested the cell cycle at the S phase byCDK2modulation, and reduced the number of anti-cyclin D1-stained HepG2 cells. Simultaneous treatment with the extract and cisplatin increased the latter's cytotoxicity, apoptotic cell death, andBAXexpression in HepG2 cells. Altogether, the results reported herein indicate thatP. ramifloraextract is a possible adjuvant to cancer therapy, which can circumvent the cisplatin-mediated resistance mechanisms in cancer cells.
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spelling Phytochemical Profile, and Antiproliferative and Proapoptotic Effects ofPouteria ramiflora(Mart.) Radlk. Leaf Extract, and Its Synergism with Cisplatin in HepG2 Cellsantitumorapoptosisflow cytometryimmunocytochemistrymedicinal plantRT-qPCRDifferent species of the genusPouteriahave been used in folk medicine for the treatment of inflammation, fever, ulcers, diabetes, and diarrhea. We analyzed the phytochemical profile of the hydroethanolic extract fromPouteria ramifloraleaves by electrospray ionization ion trap tandem mass spectrometry and high-performance liquid chromatography-diode array detection, and examined whether it alone and in combination with cisplatin interfered with cell proliferation and death processes in HepG2 (human hepatocellular carcinoma) and FGH (human gingival fibroblasts) cells. Five compounds were identified in the extract: gallic acid, myricetin-3-O-alpha-l-arabinopyranoside, quercetin-3-O-beta-d-galactopyranoside, myricetin-3-O-alpha-l-rhamnopyranoside, and myricetin-3-O-beta-d-galactopyranoside. The extract was cytotoxic to both cell lines by inducing apoptotic cell death and acted in synergy with cisplatin; such effect was stronger in HepG2 cells than in FGH cells, demonstrating some selectivity to tumor cells. In HepG2 cells, the extract exerted antiproliferative effect mediated by induction of cell cycle arrest at the S and G2/M phases. Association of the extract with cisplatin enhanced the latter's antiproliferative effect, arrested the cell cycle at the S phase byCDK2modulation, and reduced the number of anti-cyclin D1-stained HepG2 cells. Simultaneous treatment with the extract and cisplatin increased the latter's cytotoxicity, apoptotic cell death, andBAXexpression in HepG2 cells. Altogether, the results reported herein indicate thatP. ramifloraextract is a possible adjuvant to cancer therapy, which can circumvent the cisplatin-mediated resistance mechanisms in cancer cells.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Londrina State Univ UEL, Dept Gen Biol, Ctr Biol Sci, Londrina, PR, BrazilSao Paulo State Univ UNESP, Dept Organ Chem, Inst Chem, Araraquara, SP, BrazilBela Vista Campus IFMT, Fed Inst Mato Grosso, Cuiaba, MT, BrazilSao Paulo State Univ UNESP, Dept Biol Sci, Fac Pharmaceut Sci Araraquara, Araraquara, SP, BrazilSao Paulo State Univ UNESP, Expt Campus Paulista Coast, Sao Vicente, SP, BrazilClemente Estable Biol Res Inst IIBCE, Montevideo, UruguaySao Paulo State Univ UNESP, Dept Organ Chem, Inst Chem, Araraquara, SP, BrazilSao Paulo State Univ UNESP, Dept Biol Sci, Fac Pharmaceut Sci Araraquara, Araraquara, SP, BrazilSao Paulo State Univ UNESP, Expt Campus Paulista Coast, Sao Vicente, SP, BrazilFAPESP: 2009/52237-9CAPES: 001Mary Ann Liebert, IncUniversidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)Bela Vista Campus IFMTClemente Estable Biol Res Inst IIBCETuttis, KatiuskaCosta, Daryne Lu Maldonado Gomes da [UNESP]Serpeloni, Juliana MaraSantos, Lourdes Campaner dos [UNESP]Varanda, Eliana Aparecida [UNESP]Vilegas, Wagner [UNESP]Martinez-Lopez, WilnerColus, Ilce Mara de Syllos2020-12-10T20:08:12Z2020-12-10T20:08:12Z2020-08-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.1089/jmf.2020.0045Journal Of Medicinal Food. New Rochelle: Mary Ann Liebert, Inc, 12 p., 2020.1096-620Xhttp://hdl.handle.net/11449/19716410.1089/jmf.2020.0045WOS:000558255100001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Medicinal Foodinfo:eu-repo/semantics/openAccess2024-06-24T13:07:13Zoai:repositorio.unesp.br:11449/197164Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:24:14.474586Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Phytochemical Profile, and Antiproliferative and Proapoptotic Effects ofPouteria ramiflora(Mart.) Radlk. Leaf Extract, and Its Synergism with Cisplatin in HepG2 Cells
title Phytochemical Profile, and Antiproliferative and Proapoptotic Effects ofPouteria ramiflora(Mart.) Radlk. Leaf Extract, and Its Synergism with Cisplatin in HepG2 Cells
spellingShingle Phytochemical Profile, and Antiproliferative and Proapoptotic Effects ofPouteria ramiflora(Mart.) Radlk. Leaf Extract, and Its Synergism with Cisplatin in HepG2 Cells
Tuttis, Katiuska
antitumor
apoptosis
flow cytometry
immunocytochemistry
medicinal plant
RT-qPCR
title_short Phytochemical Profile, and Antiproliferative and Proapoptotic Effects ofPouteria ramiflora(Mart.) Radlk. Leaf Extract, and Its Synergism with Cisplatin in HepG2 Cells
title_full Phytochemical Profile, and Antiproliferative and Proapoptotic Effects ofPouteria ramiflora(Mart.) Radlk. Leaf Extract, and Its Synergism with Cisplatin in HepG2 Cells
title_fullStr Phytochemical Profile, and Antiproliferative and Proapoptotic Effects ofPouteria ramiflora(Mart.) Radlk. Leaf Extract, and Its Synergism with Cisplatin in HepG2 Cells
title_full_unstemmed Phytochemical Profile, and Antiproliferative and Proapoptotic Effects ofPouteria ramiflora(Mart.) Radlk. Leaf Extract, and Its Synergism with Cisplatin in HepG2 Cells
title_sort Phytochemical Profile, and Antiproliferative and Proapoptotic Effects ofPouteria ramiflora(Mart.) Radlk. Leaf Extract, and Its Synergism with Cisplatin in HepG2 Cells
author Tuttis, Katiuska
author_facet Tuttis, Katiuska
Costa, Daryne Lu Maldonado Gomes da [UNESP]
Serpeloni, Juliana Mara
Santos, Lourdes Campaner dos [UNESP]
Varanda, Eliana Aparecida [UNESP]
Vilegas, Wagner [UNESP]
Martinez-Lopez, Wilner
Colus, Ilce Mara de Syllos
author_role author
author2 Costa, Daryne Lu Maldonado Gomes da [UNESP]
Serpeloni, Juliana Mara
Santos, Lourdes Campaner dos [UNESP]
Varanda, Eliana Aparecida [UNESP]
Vilegas, Wagner [UNESP]
Martinez-Lopez, Wilner
Colus, Ilce Mara de Syllos
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Londrina (UEL)
Universidade Estadual Paulista (Unesp)
Bela Vista Campus IFMT
Clemente Estable Biol Res Inst IIBCE
dc.contributor.author.fl_str_mv Tuttis, Katiuska
Costa, Daryne Lu Maldonado Gomes da [UNESP]
Serpeloni, Juliana Mara
Santos, Lourdes Campaner dos [UNESP]
Varanda, Eliana Aparecida [UNESP]
Vilegas, Wagner [UNESP]
Martinez-Lopez, Wilner
Colus, Ilce Mara de Syllos
dc.subject.por.fl_str_mv antitumor
apoptosis
flow cytometry
immunocytochemistry
medicinal plant
RT-qPCR
topic antitumor
apoptosis
flow cytometry
immunocytochemistry
medicinal plant
RT-qPCR
description Different species of the genusPouteriahave been used in folk medicine for the treatment of inflammation, fever, ulcers, diabetes, and diarrhea. We analyzed the phytochemical profile of the hydroethanolic extract fromPouteria ramifloraleaves by electrospray ionization ion trap tandem mass spectrometry and high-performance liquid chromatography-diode array detection, and examined whether it alone and in combination with cisplatin interfered with cell proliferation and death processes in HepG2 (human hepatocellular carcinoma) and FGH (human gingival fibroblasts) cells. Five compounds were identified in the extract: gallic acid, myricetin-3-O-alpha-l-arabinopyranoside, quercetin-3-O-beta-d-galactopyranoside, myricetin-3-O-alpha-l-rhamnopyranoside, and myricetin-3-O-beta-d-galactopyranoside. The extract was cytotoxic to both cell lines by inducing apoptotic cell death and acted in synergy with cisplatin; such effect was stronger in HepG2 cells than in FGH cells, demonstrating some selectivity to tumor cells. In HepG2 cells, the extract exerted antiproliferative effect mediated by induction of cell cycle arrest at the S and G2/M phases. Association of the extract with cisplatin enhanced the latter's antiproliferative effect, arrested the cell cycle at the S phase byCDK2modulation, and reduced the number of anti-cyclin D1-stained HepG2 cells. Simultaneous treatment with the extract and cisplatin increased the latter's cytotoxicity, apoptotic cell death, andBAXexpression in HepG2 cells. Altogether, the results reported herein indicate thatP. ramifloraextract is a possible adjuvant to cancer therapy, which can circumvent the cisplatin-mediated resistance mechanisms in cancer cells.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-10T20:08:12Z
2020-12-10T20:08:12Z
2020-08-04
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1089/jmf.2020.0045
Journal Of Medicinal Food. New Rochelle: Mary Ann Liebert, Inc, 12 p., 2020.
1096-620X
http://hdl.handle.net/11449/197164
10.1089/jmf.2020.0045
WOS:000558255100001
url http://dx.doi.org/10.1089/jmf.2020.0045
http://hdl.handle.net/11449/197164
identifier_str_mv Journal Of Medicinal Food. New Rochelle: Mary Ann Liebert, Inc, 12 p., 2020.
1096-620X
10.1089/jmf.2020.0045
WOS:000558255100001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Medicinal Food
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
dc.publisher.none.fl_str_mv Mary Ann Liebert, Inc
publisher.none.fl_str_mv Mary Ann Liebert, Inc
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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