Isolation and molecular characterization of Cordyceps sp. from Bemisia tabaci (Hemiptera: Aleyrodidae) and pathogenic to Glycaspis brimblecombei (Hemiptera: Aphalaridae)

Detalhes bibliográficos
Autor(a) principal: Domingues, M. M. [UNESP]
Data de Publicação: 2022
Outros Autores: Santos, P. L. [UNESP], Gêa, B. C. C. [UNESP], Carvalho, V. R. [UNESP], Oliveira, F. N. [UNESP], Soliman, E. P., Silva, W. M., Zanuncio, J. C., Santos, V. C., Wilcken, C. F. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1519-6984.253028
http://hdl.handle.net/11449/236977
Resumo: The Brazilian forestry sector stands out for its technology, forestry management practices, social and environmental responsibility and, mainly, for its high productivity and exotic pests can reduce it. The red gum lerp psyllid Glycaspis brimblecombei (Moore, 1964) (Hemiptera: Aphalaridae) is an important pest in Eucalyptus plantations. The parasitoid Psyllaephagus bliteus (Riek, 1962) (Hymenoptera: Encyrtidae), predatory bugs and entomopathogenic fungi such as Beauveria bassiana and Metarhizium anisopliae are the natural enemies and used in the biological control of the red gum lerp psyllid. The use of entomopathogenic fungi against exotic pests is increasing in the forestry sector and the prospecting and identification of fungus isolates is important for integrated pest management. The objective of this work was the isolation and molecular identification of Cordyceps spp. And to evaluate the pathogenicity of isolates, obtained from Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) adults, against to the red gum lerp psyllid G. brimblecombei. The fungi were isolated from B. tabaci adults found in soybean and tomato crops and molecularly identified. The conidia obtained were suspended in solution with Tween 80 (0.1%) at a concentration of 1.0 × 108 conidia/mL and sprayed on ten G. brimblecombei nymphs per Eucalyptus leaf cut and placed on a hydroretentive gel inside per Petri dishes as a replication. The number of dead insects was quantified, daily, for seven days, and transferred to humid chambers. Cordyceps javanica (LCBPF 11) and C. fumosorosea (LCBPF 12 and LCBPF 63) were identified with a molecular analysis and all isolates were pathogenic to the insects and indicates that they could be used to manage G. brimblecombei and adds to reports that, normally, fungi cause greater mortality on insects of the same order as that from which they were isolated.
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spelling Isolation and molecular characterization of Cordyceps sp. from Bemisia tabaci (Hemiptera: Aleyrodidae) and pathogenic to Glycaspis brimblecombei (Hemiptera: Aphalaridae)Isolamento e caracterização molecular de Cordyceps sp. de Bemisia tabaci e patogênico para Glycaspis brimblecombei (Hemiptera: Aphalaridae)biological controlCordyceps fumosoroseaCordyceps javanicaentomopathogenic fungiEucalyptuscontrole biológicoCordyceps fumosoroseaCordyceps javanicafungos entomopatogênicosEucalyptusThe Brazilian forestry sector stands out for its technology, forestry management practices, social and environmental responsibility and, mainly, for its high productivity and exotic pests can reduce it. The red gum lerp psyllid Glycaspis brimblecombei (Moore, 1964) (Hemiptera: Aphalaridae) is an important pest in Eucalyptus plantations. The parasitoid Psyllaephagus bliteus (Riek, 1962) (Hymenoptera: Encyrtidae), predatory bugs and entomopathogenic fungi such as Beauveria bassiana and Metarhizium anisopliae are the natural enemies and used in the biological control of the red gum lerp psyllid. The use of entomopathogenic fungi against exotic pests is increasing in the forestry sector and the prospecting and identification of fungus isolates is important for integrated pest management. The objective of this work was the isolation and molecular identification of Cordyceps spp. And to evaluate the pathogenicity of isolates, obtained from Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) adults, against to the red gum lerp psyllid G. brimblecombei. The fungi were isolated from B. tabaci adults found in soybean and tomato crops and molecularly identified. The conidia obtained were suspended in solution with Tween 80 (0.1%) at a concentration of 1.0 × 108 conidia/mL and sprayed on ten G. brimblecombei nymphs per Eucalyptus leaf cut and placed on a hydroretentive gel inside per Petri dishes as a replication. The number of dead insects was quantified, daily, for seven days, and transferred to humid chambers. Cordyceps javanica (LCBPF 11) and C. fumosorosea (LCBPF 12 and LCBPF 63) were identified with a molecular analysis and all isolates were pathogenic to the insects and indicates that they could be used to manage G. brimblecombei and adds to reports that, normally, fungi cause greater mortality on insects of the same order as that from which they were isolated.O setor florestal brasileiro se destaca pela tecnologia, práticas de manejo florestal, responsabilidade social e ambiental e, principalmente, alta produtividade, mas pragas exóticas podem reduzir isto. O parasitoide Psyllaephagus bliteus (Riek, 1962) (Hymenoptera: Encyrtidae), percevejos predadores e fungos entomopatogênicos como Beauveria bassiana e Metarhizium anisopliae são inimigos naturais usados no controle biológico do psilídeo-de-concha do eucalipto Glycaspis brimblecombei (Moore, 1964) (Hemiptera: Aphalaridae), praga em plantios de eucalipto. A utilização de fungos entomopatogênicos contra pragas exóticas vem aumentando no setor florestal e a prospecção e identificação de isolados fúngicos é importante para o manejo integrado de pragas. O objetivo deste estudo foi o isolamento e identificação molecular de Cordyceps spp. e avaliar a patogenicidade dos isolados de Cordyceps javanica (LCBPF 11) e Cordyceps fumosorosea (LCBPF 12 e LCBPF 63), obtidos de adultos de Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae), ao psilídeo-de-concha G. brimblecombei. Os fungos foram isolados de adultos de B. tabaci encontrados em culturas de soja e tomate e identificados molecularmente. Os conídios foram suspensos em solução com Tween 80 (0,1%) na concentração de 1,0 x 108 conídios/mL e pulverizados sobre dez ninfas de G. brimblecombei por folha de Eucalyptus cortada e colocada sobre gel hidrorretentor no interior de placas de Petri por repetição. O número de insetos mortos foi quantificado, diariamente, por sete dias e transferidos para câmaras úmidas. Cordyceps javanica (LCBPF 11) e C. fumosorosea (LCBPF 12 e LCBPF 63) foram identificados por análise molecular e todos os isolados foram patogênicos para os insetos. Isto indica serem importantes para o manejo de G. brimblecombei e confirma relatos de que, normalmente, fungos causam maiores mortalidades em insetos da mesma ordem daquela da qual foram isolados.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista (UNESP), Faculdade de Ciências AgronômicasSuzano Papel e Celulose/Tecnologia FlorestalUniversidade Federal de Viçosa, Departamento de Engenharia FlorestalUniversidade Federal de Viçosa, Instituto de Biotecnologia Aplicada à AgropecuáriaUniversidade Estadual Paulista (UNESP), Faculdade de Ciências AgronômicasCAPES: 001Instituto Internacional de EcologiaUniversidade Estadual Paulista (UNESP)Suzano Papel e Celulose/Tecnologia FlorestalUniversidade Federal de ViçosaDomingues, M. M. [UNESP]Santos, P. L. [UNESP]Gêa, B. C. C. [UNESP]Carvalho, V. R. [UNESP]Oliveira, F. N. [UNESP]Soliman, E. P.Silva, W. M.Zanuncio, J. C.Santos, V. C.Wilcken, C. F. [UNESP]2022-10-10T13:56:24Z2022-10-10T13:56:24Z2022-04-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1519-6984.253028Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 84, p. -, 2022.1519-69841678-4375http://hdl.handle.net/11449/23697710.1590/1519-6984.253028S1519-69842024000100284S1519-69842024000100284.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Biologyinfo:eu-repo/semantics/openAccess2024-04-30T18:07:44Zoai:repositorio.unesp.br:11449/236977Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:04:40.515434Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Isolation and molecular characterization of Cordyceps sp. from Bemisia tabaci (Hemiptera: Aleyrodidae) and pathogenic to Glycaspis brimblecombei (Hemiptera: Aphalaridae)
Isolamento e caracterização molecular de Cordyceps sp. de Bemisia tabaci e patogênico para Glycaspis brimblecombei (Hemiptera: Aphalaridae)
title Isolation and molecular characterization of Cordyceps sp. from Bemisia tabaci (Hemiptera: Aleyrodidae) and pathogenic to Glycaspis brimblecombei (Hemiptera: Aphalaridae)
spellingShingle Isolation and molecular characterization of Cordyceps sp. from Bemisia tabaci (Hemiptera: Aleyrodidae) and pathogenic to Glycaspis brimblecombei (Hemiptera: Aphalaridae)
Domingues, M. M. [UNESP]
biological control
Cordyceps fumosorosea
Cordyceps javanica
entomopathogenic fungi
Eucalyptus
controle biológico
Cordyceps fumosorosea
Cordyceps javanica
fungos entomopatogênicos
Eucalyptus
title_short Isolation and molecular characterization of Cordyceps sp. from Bemisia tabaci (Hemiptera: Aleyrodidae) and pathogenic to Glycaspis brimblecombei (Hemiptera: Aphalaridae)
title_full Isolation and molecular characterization of Cordyceps sp. from Bemisia tabaci (Hemiptera: Aleyrodidae) and pathogenic to Glycaspis brimblecombei (Hemiptera: Aphalaridae)
title_fullStr Isolation and molecular characterization of Cordyceps sp. from Bemisia tabaci (Hemiptera: Aleyrodidae) and pathogenic to Glycaspis brimblecombei (Hemiptera: Aphalaridae)
title_full_unstemmed Isolation and molecular characterization of Cordyceps sp. from Bemisia tabaci (Hemiptera: Aleyrodidae) and pathogenic to Glycaspis brimblecombei (Hemiptera: Aphalaridae)
title_sort Isolation and molecular characterization of Cordyceps sp. from Bemisia tabaci (Hemiptera: Aleyrodidae) and pathogenic to Glycaspis brimblecombei (Hemiptera: Aphalaridae)
author Domingues, M. M. [UNESP]
author_facet Domingues, M. M. [UNESP]
Santos, P. L. [UNESP]
Gêa, B. C. C. [UNESP]
Carvalho, V. R. [UNESP]
Oliveira, F. N. [UNESP]
Soliman, E. P.
Silva, W. M.
Zanuncio, J. C.
Santos, V. C.
Wilcken, C. F. [UNESP]
author_role author
author2 Santos, P. L. [UNESP]
Gêa, B. C. C. [UNESP]
Carvalho, V. R. [UNESP]
Oliveira, F. N. [UNESP]
Soliman, E. P.
Silva, W. M.
Zanuncio, J. C.
Santos, V. C.
Wilcken, C. F. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Suzano Papel e Celulose/Tecnologia Florestal
Universidade Federal de Viçosa
dc.contributor.author.fl_str_mv Domingues, M. M. [UNESP]
Santos, P. L. [UNESP]
Gêa, B. C. C. [UNESP]
Carvalho, V. R. [UNESP]
Oliveira, F. N. [UNESP]
Soliman, E. P.
Silva, W. M.
Zanuncio, J. C.
Santos, V. C.
Wilcken, C. F. [UNESP]
dc.subject.por.fl_str_mv biological control
Cordyceps fumosorosea
Cordyceps javanica
entomopathogenic fungi
Eucalyptus
controle biológico
Cordyceps fumosorosea
Cordyceps javanica
fungos entomopatogênicos
Eucalyptus
topic biological control
Cordyceps fumosorosea
Cordyceps javanica
entomopathogenic fungi
Eucalyptus
controle biológico
Cordyceps fumosorosea
Cordyceps javanica
fungos entomopatogênicos
Eucalyptus
description The Brazilian forestry sector stands out for its technology, forestry management practices, social and environmental responsibility and, mainly, for its high productivity and exotic pests can reduce it. The red gum lerp psyllid Glycaspis brimblecombei (Moore, 1964) (Hemiptera: Aphalaridae) is an important pest in Eucalyptus plantations. The parasitoid Psyllaephagus bliteus (Riek, 1962) (Hymenoptera: Encyrtidae), predatory bugs and entomopathogenic fungi such as Beauveria bassiana and Metarhizium anisopliae are the natural enemies and used in the biological control of the red gum lerp psyllid. The use of entomopathogenic fungi against exotic pests is increasing in the forestry sector and the prospecting and identification of fungus isolates is important for integrated pest management. The objective of this work was the isolation and molecular identification of Cordyceps spp. And to evaluate the pathogenicity of isolates, obtained from Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) adults, against to the red gum lerp psyllid G. brimblecombei. The fungi were isolated from B. tabaci adults found in soybean and tomato crops and molecularly identified. The conidia obtained were suspended in solution with Tween 80 (0.1%) at a concentration of 1.0 × 108 conidia/mL and sprayed on ten G. brimblecombei nymphs per Eucalyptus leaf cut and placed on a hydroretentive gel inside per Petri dishes as a replication. The number of dead insects was quantified, daily, for seven days, and transferred to humid chambers. Cordyceps javanica (LCBPF 11) and C. fumosorosea (LCBPF 12 and LCBPF 63) were identified with a molecular analysis and all isolates were pathogenic to the insects and indicates that they could be used to manage G. brimblecombei and adds to reports that, normally, fungi cause greater mortality on insects of the same order as that from which they were isolated.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-10T13:56:24Z
2022-10-10T13:56:24Z
2022-04-29
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1519-6984.253028
Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 84, p. -, 2022.
1519-6984
1678-4375
http://hdl.handle.net/11449/236977
10.1590/1519-6984.253028
S1519-69842024000100284
S1519-69842024000100284.pdf
url http://dx.doi.org/10.1590/1519-6984.253028
http://hdl.handle.net/11449/236977
identifier_str_mv Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 84, p. -, 2022.
1519-6984
1678-4375
10.1590/1519-6984.253028
S1519-69842024000100284
S1519-69842024000100284.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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application/pdf
dc.publisher.none.fl_str_mv Instituto Internacional de Ecologia
publisher.none.fl_str_mv Instituto Internacional de Ecologia
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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