Effective Pre-Clinical Treatment of Fish Envenoming with Polyclonal Antiserum
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijms24098338 http://hdl.handle.net/11449/247373 |
Resumo: | Envenomation by venomous fish, although not always fatal, is capable of causing damage to homeostasis by activating the inflammatory process, with the formation of edema, excruciating pain, necrosis that is difficult to heal, as well as hemodynamic and cardiorespiratory changes. Despite the wide variety of pharmacological treatments used to manage acute symptoms, none are effective in controlling envenomation. Knowing the essential role of neutralizing polyclonal antibodies in the treatment of envenoming for other species, such as snakes, this work aimed to produce a polyclonal antiserum in mice and test its ability to neutralize the main toxic effects induced by the venoms of the main venomous Brazilian fish. We found that the antiserum recognizes the main toxins present in the different venoms of Thalassophryne nattereri, Scorpaena plumieri, Potamotrygon gr. Orbignyi, and Cathorops spixii and was effective in pre-incubation trials. In an independent test, the antiserum applied immediately to the topical application of T. nattereri, P. gr orbygnyi, and C. spixii venoms completely abolished the toxic effects on the microcirculation, preventing alterations such as arteriolar contraction, slowing of blood flow in postcapillary venules, venular stasis, myofibrillar hypercontraction, and increased leukocyte rolling and adherence. The edematogenic and nociceptive activities induced by these venoms were also neutralized by the immediate application of the antiserum. Importantly, the antiserum prevented the acute inflammatory response in the lungs induced by the S. plumieri venom. The success of antiserum containing neutralizing polyclonal antibodies in controlling the toxic effects induced by different venoms offers a new strategy for the treatment of fish envenomation in Brazil. |
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Effective Pre-Clinical Treatment of Fish Envenoming with Polyclonal AntiserumCathrops spixiifish venomneutralizing antibodypolyclonal antiserumPotamotrygon gr orbignyiScorpaena plumieriThalassophryne nattereritoxic effectsEnvenomation by venomous fish, although not always fatal, is capable of causing damage to homeostasis by activating the inflammatory process, with the formation of edema, excruciating pain, necrosis that is difficult to heal, as well as hemodynamic and cardiorespiratory changes. Despite the wide variety of pharmacological treatments used to manage acute symptoms, none are effective in controlling envenomation. Knowing the essential role of neutralizing polyclonal antibodies in the treatment of envenoming for other species, such as snakes, this work aimed to produce a polyclonal antiserum in mice and test its ability to neutralize the main toxic effects induced by the venoms of the main venomous Brazilian fish. We found that the antiserum recognizes the main toxins present in the different venoms of Thalassophryne nattereri, Scorpaena plumieri, Potamotrygon gr. Orbignyi, and Cathorops spixii and was effective in pre-incubation trials. In an independent test, the antiserum applied immediately to the topical application of T. nattereri, P. gr orbygnyi, and C. spixii venoms completely abolished the toxic effects on the microcirculation, preventing alterations such as arteriolar contraction, slowing of blood flow in postcapillary venules, venular stasis, myofibrillar hypercontraction, and increased leukocyte rolling and adherence. The edematogenic and nociceptive activities induced by these venoms were also neutralized by the immediate application of the antiserum. Importantly, the antiserum prevented the acute inflammatory response in the lungs induced by the S. plumieri venom. The success of antiserum containing neutralizing polyclonal antibodies in controlling the toxic effects induced by different venoms offers a new strategy for the treatment of fish envenomation in Brazil.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Immunoregulation Unit of Laboratory of Applied Toxinology (CeTICs/FAPESP) Butantan InstituteFaculdade de Medicina Universidade Estadual PaulistaEnvironmental Sciences Federal University of Tocantins, Campus of PalmasFaculdade de Medicina Universidade Estadual PaulistaButantan InstituteUniversidade Estadual Paulista (UNESP)Federal University of TocantinsLopes Ferreira, MonicaFalcão, Maria Alice PimentelBruni, Fernanda MirianeHaddad, Vidal [UNESP]Marques, Elineide EugênioSeibert, Carla SimoneLima, Carla2023-07-29T13:14:16Z2023-07-29T13:14:16Z2023-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ijms24098338International Journal of Molecular Sciences, v. 24, n. 9, 2023.1422-00671661-6596http://hdl.handle.net/11449/24737310.3390/ijms240983382-s2.0-85159271978Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciencesinfo:eu-repo/semantics/openAccess2023-07-29T13:14:16Zoai:repositorio.unesp.br:11449/247373Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:14:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effective Pre-Clinical Treatment of Fish Envenoming with Polyclonal Antiserum |
title |
Effective Pre-Clinical Treatment of Fish Envenoming with Polyclonal Antiserum |
spellingShingle |
Effective Pre-Clinical Treatment of Fish Envenoming with Polyclonal Antiserum Lopes Ferreira, Monica Cathrops spixii fish venom neutralizing antibody polyclonal antiserum Potamotrygon gr orbignyi Scorpaena plumieri Thalassophryne nattereri toxic effects |
title_short |
Effective Pre-Clinical Treatment of Fish Envenoming with Polyclonal Antiserum |
title_full |
Effective Pre-Clinical Treatment of Fish Envenoming with Polyclonal Antiserum |
title_fullStr |
Effective Pre-Clinical Treatment of Fish Envenoming with Polyclonal Antiserum |
title_full_unstemmed |
Effective Pre-Clinical Treatment of Fish Envenoming with Polyclonal Antiserum |
title_sort |
Effective Pre-Clinical Treatment of Fish Envenoming with Polyclonal Antiserum |
author |
Lopes Ferreira, Monica |
author_facet |
Lopes Ferreira, Monica Falcão, Maria Alice Pimentel Bruni, Fernanda Miriane Haddad, Vidal [UNESP] Marques, Elineide Eugênio Seibert, Carla Simone Lima, Carla |
author_role |
author |
author2 |
Falcão, Maria Alice Pimentel Bruni, Fernanda Miriane Haddad, Vidal [UNESP] Marques, Elineide Eugênio Seibert, Carla Simone Lima, Carla |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Butantan Institute Universidade Estadual Paulista (UNESP) Federal University of Tocantins |
dc.contributor.author.fl_str_mv |
Lopes Ferreira, Monica Falcão, Maria Alice Pimentel Bruni, Fernanda Miriane Haddad, Vidal [UNESP] Marques, Elineide Eugênio Seibert, Carla Simone Lima, Carla |
dc.subject.por.fl_str_mv |
Cathrops spixii fish venom neutralizing antibody polyclonal antiserum Potamotrygon gr orbignyi Scorpaena plumieri Thalassophryne nattereri toxic effects |
topic |
Cathrops spixii fish venom neutralizing antibody polyclonal antiserum Potamotrygon gr orbignyi Scorpaena plumieri Thalassophryne nattereri toxic effects |
description |
Envenomation by venomous fish, although not always fatal, is capable of causing damage to homeostasis by activating the inflammatory process, with the formation of edema, excruciating pain, necrosis that is difficult to heal, as well as hemodynamic and cardiorespiratory changes. Despite the wide variety of pharmacological treatments used to manage acute symptoms, none are effective in controlling envenomation. Knowing the essential role of neutralizing polyclonal antibodies in the treatment of envenoming for other species, such as snakes, this work aimed to produce a polyclonal antiserum in mice and test its ability to neutralize the main toxic effects induced by the venoms of the main venomous Brazilian fish. We found that the antiserum recognizes the main toxins present in the different venoms of Thalassophryne nattereri, Scorpaena plumieri, Potamotrygon gr. Orbignyi, and Cathorops spixii and was effective in pre-incubation trials. In an independent test, the antiserum applied immediately to the topical application of T. nattereri, P. gr orbygnyi, and C. spixii venoms completely abolished the toxic effects on the microcirculation, preventing alterations such as arteriolar contraction, slowing of blood flow in postcapillary venules, venular stasis, myofibrillar hypercontraction, and increased leukocyte rolling and adherence. The edematogenic and nociceptive activities induced by these venoms were also neutralized by the immediate application of the antiserum. Importantly, the antiserum prevented the acute inflammatory response in the lungs induced by the S. plumieri venom. The success of antiserum containing neutralizing polyclonal antibodies in controlling the toxic effects induced by different venoms offers a new strategy for the treatment of fish envenomation in Brazil. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:14:16Z 2023-07-29T13:14:16Z 2023-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijms24098338 International Journal of Molecular Sciences, v. 24, n. 9, 2023. 1422-0067 1661-6596 http://hdl.handle.net/11449/247373 10.3390/ijms24098338 2-s2.0-85159271978 |
url |
http://dx.doi.org/10.3390/ijms24098338 http://hdl.handle.net/11449/247373 |
identifier_str_mv |
International Journal of Molecular Sciences, v. 24, n. 9, 2023. 1422-0067 1661-6596 10.3390/ijms24098338 2-s2.0-85159271978 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Molecular Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965300939030528 |