Atividade antipsoriática de extratos de tropaeolum majus (tropaeolaceae) padronizados em antocianinas sobre lesões Psoriasiformes induzidas em camundongos com imiquimode
Main Author: | |
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Publication Date: | 2017 |
Format: | Master thesis |
Language: | por |
Source: | Repositório Universitário da Ânima (RUNA) |
Download full: | https://repositorio.animaeducacao.com.br/handle/ANIMA/3098 |
Summary: | Introduction: Two components served as the basis for the development of this study. On the one hand, a disease whose treatment is palliative, limited in efficacy and related to adverse effects; psoriasis. On the other hand, a plant with a history in popular medicine (Tropaeolum majus, known mainly as capuchinha) for which there was a body of literature that allowed to hypothesize that any product derived from it could have a therapeutic effect on psoriatic lesions. Objective: To evaluate the antipsoriatic potential of Tropaeolum majus extracts on psoriasis-like wounds induced in mice with imiquimod. Methods: The ethyl acetate, ethanolic and hydroethanolic extracts were obtained from the plant’s flowers by exhaustive maceration. Concentration of anthocyanins in the extracts was determined by the differential pH method. Balb-c mice (n = 12) groups were formed: normal and negative control groups treated with water and 10% glycerol, positive control treated with 0.05 mg/mL clobetasol and groups receiving extract (50 mg/mL). Wounds were induced on the back of mice. The treatments were daily for five days. Two hours after the administration of the last doses, half of each group was euthanized serving to collect cutaneous tissue for analyzes of concentration of TNF-α, IL-1β, ERK phosphorylation, lipid peroxidation, protein carbonylation and reduced glutathione (GSH). The other half of animals were kept alive and served to determine the time required for wounds’ clearance. Results: The concentration of anthocyanins was 0.62 ± 0.09; 6.96 ± 0.90 and 10.86 ± 0.64 mg/g in the ethyl acetate, ethanolic and hydroethanolic extracts, respectively. The severity of wounds was reduced by 35% and up to 45% in mice treated with ethanolic and hydroethanolic extract, as well as time for wounds’ clearance (~ twice and > 3 times), respectively. The production of TNF-α and IL-1β was normalized by ethanolic and hydroethanolic extracts, as well as lipid peroxidation and protein carbonylation. Only these extracts recovered GSH levels, being the most potent activity caused by the hydroethanolic extract. Conclusion: Data suggested a relationship between antipsoriatic activity and anthocyanins. The most potent antipsoriatic activity was caused by the hydroethanolic extract, which had the highest concentration of anthocyanins among the extracts evaluated. |
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Atividade antipsoriática de extratos de tropaeolum majus (tropaeolaceae) padronizados em antocianinas sobre lesões Psoriasiformes induzidas em camundongos com imiquimodeTropaeolum majusAntocianinasAntipsoriáticoIntroduction: Two components served as the basis for the development of this study. On the one hand, a disease whose treatment is palliative, limited in efficacy and related to adverse effects; psoriasis. On the other hand, a plant with a history in popular medicine (Tropaeolum majus, known mainly as capuchinha) for which there was a body of literature that allowed to hypothesize that any product derived from it could have a therapeutic effect on psoriatic lesions. Objective: To evaluate the antipsoriatic potential of Tropaeolum majus extracts on psoriasis-like wounds induced in mice with imiquimod. Methods: The ethyl acetate, ethanolic and hydroethanolic extracts were obtained from the plant’s flowers by exhaustive maceration. Concentration of anthocyanins in the extracts was determined by the differential pH method. Balb-c mice (n = 12) groups were formed: normal and negative control groups treated with water and 10% glycerol, positive control treated with 0.05 mg/mL clobetasol and groups receiving extract (50 mg/mL). Wounds were induced on the back of mice. The treatments were daily for five days. Two hours after the administration of the last doses, half of each group was euthanized serving to collect cutaneous tissue for analyzes of concentration of TNF-α, IL-1β, ERK phosphorylation, lipid peroxidation, protein carbonylation and reduced glutathione (GSH). The other half of animals were kept alive and served to determine the time required for wounds’ clearance. Results: The concentration of anthocyanins was 0.62 ± 0.09; 6.96 ± 0.90 and 10.86 ± 0.64 mg/g in the ethyl acetate, ethanolic and hydroethanolic extracts, respectively. The severity of wounds was reduced by 35% and up to 45% in mice treated with ethanolic and hydroethanolic extract, as well as time for wounds’ clearance (~ twice and > 3 times), respectively. The production of TNF-α and IL-1β was normalized by ethanolic and hydroethanolic extracts, as well as lipid peroxidation and protein carbonylation. Only these extracts recovered GSH levels, being the most potent activity caused by the hydroethanolic extract. Conclusion: Data suggested a relationship between antipsoriatic activity and anthocyanins. The most potent antipsoriatic activity was caused by the hydroethanolic extract, which had the highest concentration of anthocyanins among the extracts evaluated.Introdução: Dois componentes serviram de base ao desenvolvimento deste estudo. De um lado, uma doença cujo tratamento é paliativo, limitado em eficácia e relacionado com efeitos adversos; a psoríase. De outro lado, uma planta com histórico na medicina popular (Tropaeolum majus, mais conhecida como capuchinha) para qual existia um corpo de literatura que permitia hipotetizar que algum produto dela derivado poderia ter efeito terapêutico sobre lesões psoriáticas. Objetivo: Avaliar o potencial antipsoriático de extratos de capuchinha sobre lesões psoriasiformes induzidas em camundongos com imiquimode. Métodos: Os extratos acetato de etila, etanólico e hidroetanólico foram obtidos das flores da planta por maceração exaustiva. A concentração de antocianinas nos extratos foi determinada pelo método do pH diferencial. Foram formados grupos de camundongos Balb-c (n=12): grupo normal e controle negativo tratados com água e glicerina 10%, controle positivo tratado com clobetasol 0,05 mg/mL e grupos recebendo extrato (50 mg/mL). As lesões foram induzidas no dorso dos animais. Os tratamentos foram diários por cinco dias. Duas horas após a administração das últimas doses, metade de cada grupo foi eutanasiada servindo para coleta de tecido cutâneo para análises da concentração de TNF-α, IL-1β, fosforilação da proteína ERK, peroxidação lipídica, carbonilação proteica e glutationa reduzida (GSH). A outra metade dos animais mantidos vivos serviu para determinar o tempo necessário ao desaparecimento das lesões. Resultados: A concentração de antocianinas foi 0,62 ± 0,09; 6,96 ± 0,90 e 10,86 ± 0,64 mg/g nos extratos acetato de etila, etanólico e hidroetanólico, respectivamente. A severidade das lesões foi diminuída 35% e até 45% em animais tratados com extrato etanólico e hidroetanólico, assim como o tempo ao desaparecimento das lesões (~ 2 vezes e > 3 vezes), respectivamente. A produção de TNF-α, IL-1β foi normalizada pelos extratos etanólico e hidroetanólico, assim como a peroxidação lipídica e carbonilação proteica. Somente estes extratos recuperaram os níveis de GSH, sendo esta atividade mais potente para o extrato hidroetanólico. Conclusão: Os dados obtidos sugeriram que a atividade antipsoriática dos extratos eficazes de capuchinha foi associada ao conteúdo de antocianinas encontrado nos mesmos. A atividade antipsoriática mais potente foi causada pelo extrato hidroetanólico, que apresentou a concentração mais alta de antocianinas entre os extratos avaliados.Kviecinski, Maicon RobertoFerreira, Juliana Barreto dos Santos Silva2018-02-21T16:04:49Z2020-11-26T20:58:50Z2018-02-21T16:04:49Z2020-11-26T20:58:50Z2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis99 f.application/pdfhttps://repositorio.animaeducacao.com.br/handle/ANIMA/3098Programa de Pós-Graduação em Ciência da SaúdePalhoçaAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessporreponame:Repositório Universitário da Ânima (RUNA)instname:Ânima Educaçãoinstacron:Ânima2020-12-01T16:48:17Zoai:repositorio.animaeducacao.com.br:ANIMA/3098Repositório InstitucionalPRIhttps://repositorio.animaeducacao.com.br/oai/requestcontato@animaeducacao.com.bropendoar:2020-12-01T16:48:17Repositório Universitário da Ânima (RUNA) - Ânima Educaçãofalse |
dc.title.none.fl_str_mv |
Atividade antipsoriática de extratos de tropaeolum majus (tropaeolaceae) padronizados em antocianinas sobre lesões Psoriasiformes induzidas em camundongos com imiquimode |
title |
Atividade antipsoriática de extratos de tropaeolum majus (tropaeolaceae) padronizados em antocianinas sobre lesões Psoriasiformes induzidas em camundongos com imiquimode |
spellingShingle |
Atividade antipsoriática de extratos de tropaeolum majus (tropaeolaceae) padronizados em antocianinas sobre lesões Psoriasiformes induzidas em camundongos com imiquimode Ferreira, Juliana Barreto dos Santos Silva Tropaeolum majus Antocianinas Antipsoriático |
title_short |
Atividade antipsoriática de extratos de tropaeolum majus (tropaeolaceae) padronizados em antocianinas sobre lesões Psoriasiformes induzidas em camundongos com imiquimode |
title_full |
Atividade antipsoriática de extratos de tropaeolum majus (tropaeolaceae) padronizados em antocianinas sobre lesões Psoriasiformes induzidas em camundongos com imiquimode |
title_fullStr |
Atividade antipsoriática de extratos de tropaeolum majus (tropaeolaceae) padronizados em antocianinas sobre lesões Psoriasiformes induzidas em camundongos com imiquimode |
title_full_unstemmed |
Atividade antipsoriática de extratos de tropaeolum majus (tropaeolaceae) padronizados em antocianinas sobre lesões Psoriasiformes induzidas em camundongos com imiquimode |
title_sort |
Atividade antipsoriática de extratos de tropaeolum majus (tropaeolaceae) padronizados em antocianinas sobre lesões Psoriasiformes induzidas em camundongos com imiquimode |
author |
Ferreira, Juliana Barreto dos Santos Silva |
author_facet |
Ferreira, Juliana Barreto dos Santos Silva |
author_role |
author |
dc.contributor.none.fl_str_mv |
Kviecinski, Maicon Roberto |
dc.contributor.author.fl_str_mv |
Ferreira, Juliana Barreto dos Santos Silva |
dc.subject.por.fl_str_mv |
Tropaeolum majus Antocianinas Antipsoriático |
topic |
Tropaeolum majus Antocianinas Antipsoriático |
description |
Introduction: Two components served as the basis for the development of this study. On the one hand, a disease whose treatment is palliative, limited in efficacy and related to adverse effects; psoriasis. On the other hand, a plant with a history in popular medicine (Tropaeolum majus, known mainly as capuchinha) for which there was a body of literature that allowed to hypothesize that any product derived from it could have a therapeutic effect on psoriatic lesions. Objective: To evaluate the antipsoriatic potential of Tropaeolum majus extracts on psoriasis-like wounds induced in mice with imiquimod. Methods: The ethyl acetate, ethanolic and hydroethanolic extracts were obtained from the plant’s flowers by exhaustive maceration. Concentration of anthocyanins in the extracts was determined by the differential pH method. Balb-c mice (n = 12) groups were formed: normal and negative control groups treated with water and 10% glycerol, positive control treated with 0.05 mg/mL clobetasol and groups receiving extract (50 mg/mL). Wounds were induced on the back of mice. The treatments were daily for five days. Two hours after the administration of the last doses, half of each group was euthanized serving to collect cutaneous tissue for analyzes of concentration of TNF-α, IL-1β, ERK phosphorylation, lipid peroxidation, protein carbonylation and reduced glutathione (GSH). The other half of animals were kept alive and served to determine the time required for wounds’ clearance. Results: The concentration of anthocyanins was 0.62 ± 0.09; 6.96 ± 0.90 and 10.86 ± 0.64 mg/g in the ethyl acetate, ethanolic and hydroethanolic extracts, respectively. The severity of wounds was reduced by 35% and up to 45% in mice treated with ethanolic and hydroethanolic extract, as well as time for wounds’ clearance (~ twice and > 3 times), respectively. The production of TNF-α and IL-1β was normalized by ethanolic and hydroethanolic extracts, as well as lipid peroxidation and protein carbonylation. Only these extracts recovered GSH levels, being the most potent activity caused by the hydroethanolic extract. Conclusion: Data suggested a relationship between antipsoriatic activity and anthocyanins. The most potent antipsoriatic activity was caused by the hydroethanolic extract, which had the highest concentration of anthocyanins among the extracts evaluated. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2018-02-21T16:04:49Z 2018-02-21T16:04:49Z 2020-11-26T20:58:50Z 2020-11-26T20:58:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.animaeducacao.com.br/handle/ANIMA/3098 |
url |
https://repositorio.animaeducacao.com.br/handle/ANIMA/3098 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Programa de Pós-Graduação em Ciência da Saúde |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
99 f. application/pdf |
dc.coverage.none.fl_str_mv |
Palhoça |
dc.source.none.fl_str_mv |
reponame:Repositório Universitário da Ânima (RUNA) instname:Ânima Educação instacron:Ânima |
instname_str |
Ânima Educação |
instacron_str |
Ânima |
institution |
Ânima |
reponame_str |
Repositório Universitário da Ânima (RUNA) |
collection |
Repositório Universitário da Ânima (RUNA) |
repository.name.fl_str_mv |
Repositório Universitário da Ânima (RUNA) - Ânima Educação |
repository.mail.fl_str_mv |
contato@animaeducacao.com.br |
_version_ |
1767415831297261568 |