Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract
Main Author: | |
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Publication Date: | 2019 |
Other Authors: | , , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Anais da Academia Brasileira de Ciências (Online) |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700617 |
Summary: | Abstract: Hymenaea courbaril has been used to treat different diseases, although its properties are yet to be scientifically validated. The objective of this study was to determine the cytotoxicity, genotoxicity, antigenotoxicity and antioxidant potentials of hydroethanolic extract from H. courbaril seeds. Therefore, for the cytotoxicity test an anti-melanoma assay was performed in B16F10 strain cells. The genotoxicity and antigenotoxicity was evaluated in bone marrow cells (Permit number: 002/2010) of mice, the antioxidant activity was determined by the DPPH test and the total flavonoid content was also determined. The hydroethanolic extract showed antigenotoxic effect and antioxidant activity. It was verified that total flavonoid content was 442.25±18.03 mg RE/g dry extract. HPLC-PAD chromatogram revealed presence of flavones as majority compound in evaluated extract. The results allowed us to also infer that the hydroethanolic extract from seeds shows cytotoxic activity against B16F10 melanoma cells line and it has dose-and-time-dependency. |
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Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extractB16F10 melanoma cellsmicronucleus testchromosomal aberrationDPPH radical scavengingHPLC-DADAbstract: Hymenaea courbaril has been used to treat different diseases, although its properties are yet to be scientifically validated. The objective of this study was to determine the cytotoxicity, genotoxicity, antigenotoxicity and antioxidant potentials of hydroethanolic extract from H. courbaril seeds. Therefore, for the cytotoxicity test an anti-melanoma assay was performed in B16F10 strain cells. The genotoxicity and antigenotoxicity was evaluated in bone marrow cells (Permit number: 002/2010) of mice, the antioxidant activity was determined by the DPPH test and the total flavonoid content was also determined. The hydroethanolic extract showed antigenotoxic effect and antioxidant activity. It was verified that total flavonoid content was 442.25±18.03 mg RE/g dry extract. HPLC-PAD chromatogram revealed presence of flavones as majority compound in evaluated extract. The results allowed us to also infer that the hydroethanolic extract from seeds shows cytotoxic activity against B16F10 melanoma cells line and it has dose-and-time-dependency.Academia Brasileira de Ciências2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700617Anais da Academia Brasileira de Ciências v.91 n.4 2019reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201920180446info:eu-repo/semantics/openAccessSPERA,KAMILLE D.FIGUEIREDO,PATRÍCIA A.SANTOS,PAMELA C.EBARBOSA,FERNANDO C.ALVES,CAIO P.DOKKEDAL,ANNE LÍGIASALDANHA,LUIZ LEONARDOSILVA,LUCIANA P.FIGUEIREDO,CARLOS ROGÉRIOFERREIRA,PAULO CESARSILVA,REGILDO M.G. DAeng2019-12-10T00:00:00Zoai:scielo:S0001-37652019000700617Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2019-12-10T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract |
title |
Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract |
spellingShingle |
Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract SPERA,KAMILLE D. B16F10 melanoma cells micronucleus test chromosomal aberration DPPH radical scavenging HPLC-DAD |
title_short |
Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract |
title_full |
Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract |
title_fullStr |
Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract |
title_full_unstemmed |
Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract |
title_sort |
Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract |
author |
SPERA,KAMILLE D. |
author_facet |
SPERA,KAMILLE D. FIGUEIREDO,PATRÍCIA A. SANTOS,PAMELA C.E BARBOSA,FERNANDO C. ALVES,CAIO P. DOKKEDAL,ANNE LÍGIA SALDANHA,LUIZ LEONARDO SILVA,LUCIANA P. FIGUEIREDO,CARLOS ROGÉRIO FERREIRA,PAULO CESAR SILVA,REGILDO M.G. DA |
author_role |
author |
author2 |
FIGUEIREDO,PATRÍCIA A. SANTOS,PAMELA C.E BARBOSA,FERNANDO C. ALVES,CAIO P. DOKKEDAL,ANNE LÍGIA SALDANHA,LUIZ LEONARDO SILVA,LUCIANA P. FIGUEIREDO,CARLOS ROGÉRIO FERREIRA,PAULO CESAR SILVA,REGILDO M.G. DA |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
SPERA,KAMILLE D. FIGUEIREDO,PATRÍCIA A. SANTOS,PAMELA C.E BARBOSA,FERNANDO C. ALVES,CAIO P. DOKKEDAL,ANNE LÍGIA SALDANHA,LUIZ LEONARDO SILVA,LUCIANA P. FIGUEIREDO,CARLOS ROGÉRIO FERREIRA,PAULO CESAR SILVA,REGILDO M.G. DA |
dc.subject.por.fl_str_mv |
B16F10 melanoma cells micronucleus test chromosomal aberration DPPH radical scavenging HPLC-DAD |
topic |
B16F10 melanoma cells micronucleus test chromosomal aberration DPPH radical scavenging HPLC-DAD |
description |
Abstract: Hymenaea courbaril has been used to treat different diseases, although its properties are yet to be scientifically validated. The objective of this study was to determine the cytotoxicity, genotoxicity, antigenotoxicity and antioxidant potentials of hydroethanolic extract from H. courbaril seeds. Therefore, for the cytotoxicity test an anti-melanoma assay was performed in B16F10 strain cells. The genotoxicity and antigenotoxicity was evaluated in bone marrow cells (Permit number: 002/2010) of mice, the antioxidant activity was determined by the DPPH test and the total flavonoid content was also determined. The hydroethanolic extract showed antigenotoxic effect and antioxidant activity. It was verified that total flavonoid content was 442.25±18.03 mg RE/g dry extract. HPLC-PAD chromatogram revealed presence of flavones as majority compound in evaluated extract. The results allowed us to also infer that the hydroethanolic extract from seeds shows cytotoxic activity against B16F10 melanoma cells line and it has dose-and-time-dependency. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700617 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700617 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765201920180446 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.91 n.4 2019 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302868112801792 |