Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos de neuro-psiquiatria (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2017000100030 |
Resumo: | ABSTRACT Alcohol consumption aggravates injuries caused by ischemia. Many molecular mechanisms are involved in the pathophysiology of cerebral ischemia, including neurotransmitter expression, which is regulated by microRNAs. Objective: To evaluate the microRNA-219 and NMDA expression in brain tissue and blood of animals subjected to cerebral ischemia associated with alcoholism. Methods: Fifty Wistar rats were divided into groups: control, sham, ischemic, alcoholic, and ischemic plus alcoholic. The expression of microRNA-219 and NMDA were analyzed by real-time PCR. Results: When compared to the control group, the microRNA-219 in brain tissue was less expressed in the ischemic, alcoholic, and ischemic plus alcoholic groups. In the blood, this microRNA had lower expression in alcoholic and ischemic plus alcoholic groups. In the brain tissue the NMDA gene expression was greater in the ischemic, alcoholic, and ischemic plus alcoholic groups. Conclusion: A possible modulation of NMDA by microRNA-219 was observed with an inverse correlation between them. |
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Arquivos de neuro-psiquiatria (Online) |
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Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholismbrain ischemiaalcoholismN-MethylaspartatemicroRNAsABSTRACT Alcohol consumption aggravates injuries caused by ischemia. Many molecular mechanisms are involved in the pathophysiology of cerebral ischemia, including neurotransmitter expression, which is regulated by microRNAs. Objective: To evaluate the microRNA-219 and NMDA expression in brain tissue and blood of animals subjected to cerebral ischemia associated with alcoholism. Methods: Fifty Wistar rats were divided into groups: control, sham, ischemic, alcoholic, and ischemic plus alcoholic. The expression of microRNA-219 and NMDA were analyzed by real-time PCR. Results: When compared to the control group, the microRNA-219 in brain tissue was less expressed in the ischemic, alcoholic, and ischemic plus alcoholic groups. In the blood, this microRNA had lower expression in alcoholic and ischemic plus alcoholic groups. In the brain tissue the NMDA gene expression was greater in the ischemic, alcoholic, and ischemic plus alcoholic groups. Conclusion: A possible modulation of NMDA by microRNA-219 was observed with an inverse correlation between them.Academia Brasileira de Neurologia - ABNEURO2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2017000100030Arquivos de Neuro-Psiquiatria v.75 n.1 2017reponame:Arquivos de neuro-psiquiatria (Online)instname:Academia Brasileira de Neurologiainstacron:ABNEURO10.1590/0004-282x20160188info:eu-repo/semantics/openAccessSilva,Cristiane IozziNovais,Paulo CézarRodrigues,Andressa RomualdoCarvalho,Camila A.M.Colli,Benedicto OscarCarlotti Jr.,Carlos GilbertoTirapelli,Luís FernandoTirapelli,Daniela P.C.eng2017-01-13T00:00:00Zoai:scielo:S0004-282X2017000100030Revistahttp://www.scielo.br/anphttps://old.scielo.br/oai/scielo-oai.php||revista.arquivos@abneuro.org1678-42270004-282Xopendoar:2017-01-13T00:00Arquivos de neuro-psiquiatria (Online) - Academia Brasileira de Neurologiafalse |
dc.title.none.fl_str_mv |
Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism |
title |
Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism |
spellingShingle |
Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism Silva,Cristiane Iozzi brain ischemia alcoholism N-Methylaspartate microRNAs |
title_short |
Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism |
title_full |
Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism |
title_fullStr |
Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism |
title_full_unstemmed |
Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism |
title_sort |
Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism |
author |
Silva,Cristiane Iozzi |
author_facet |
Silva,Cristiane Iozzi Novais,Paulo Cézar Rodrigues,Andressa Romualdo Carvalho,Camila A.M. Colli,Benedicto Oscar Carlotti Jr.,Carlos Gilberto Tirapelli,Luís Fernando Tirapelli,Daniela P.C. |
author_role |
author |
author2 |
Novais,Paulo Cézar Rodrigues,Andressa Romualdo Carvalho,Camila A.M. Colli,Benedicto Oscar Carlotti Jr.,Carlos Gilberto Tirapelli,Luís Fernando Tirapelli,Daniela P.C. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva,Cristiane Iozzi Novais,Paulo Cézar Rodrigues,Andressa Romualdo Carvalho,Camila A.M. Colli,Benedicto Oscar Carlotti Jr.,Carlos Gilberto Tirapelli,Luís Fernando Tirapelli,Daniela P.C. |
dc.subject.por.fl_str_mv |
brain ischemia alcoholism N-Methylaspartate microRNAs |
topic |
brain ischemia alcoholism N-Methylaspartate microRNAs |
description |
ABSTRACT Alcohol consumption aggravates injuries caused by ischemia. Many molecular mechanisms are involved in the pathophysiology of cerebral ischemia, including neurotransmitter expression, which is regulated by microRNAs. Objective: To evaluate the microRNA-219 and NMDA expression in brain tissue and blood of animals subjected to cerebral ischemia associated with alcoholism. Methods: Fifty Wistar rats were divided into groups: control, sham, ischemic, alcoholic, and ischemic plus alcoholic. The expression of microRNA-219 and NMDA were analyzed by real-time PCR. Results: When compared to the control group, the microRNA-219 in brain tissue was less expressed in the ischemic, alcoholic, and ischemic plus alcoholic groups. In the blood, this microRNA had lower expression in alcoholic and ischemic plus alcoholic groups. In the brain tissue the NMDA gene expression was greater in the ischemic, alcoholic, and ischemic plus alcoholic groups. Conclusion: A possible modulation of NMDA by microRNA-219 was observed with an inverse correlation between them. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2017000100030 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2017000100030 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0004-282x20160188 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Neurologia - ABNEURO |
publisher.none.fl_str_mv |
Academia Brasileira de Neurologia - ABNEURO |
dc.source.none.fl_str_mv |
Arquivos de Neuro-Psiquiatria v.75 n.1 2017 reponame:Arquivos de neuro-psiquiatria (Online) instname:Academia Brasileira de Neurologia instacron:ABNEURO |
instname_str |
Academia Brasileira de Neurologia |
instacron_str |
ABNEURO |
institution |
ABNEURO |
reponame_str |
Arquivos de neuro-psiquiatria (Online) |
collection |
Arquivos de neuro-psiquiatria (Online) |
repository.name.fl_str_mv |
Arquivos de neuro-psiquiatria (Online) - Academia Brasileira de Neurologia |
repository.mail.fl_str_mv |
||revista.arquivos@abneuro.org |
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1754212781713784832 |