Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism

Detalhes bibliográficos
Autor(a) principal: Schiavoni,Vagner Sarraipo
Data de Publicação: 2018
Outros Autores: Silva,Jairo Pinheiro da, Lizarte Neto,Fermino Sanches, Assis,Múcio Luiz Cirino de, Tazima,Maria de Fátima Galli Sorita, Carvalho,Camila Albuquerque Melo de, Tirapelli,Daniela Pretti da Cunha, Carlotti Jr,Carlos Gilberto, Colli,Benedicto Oscar, Tirapelli,Luis Fernando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000800652
Resumo: Abstract Purpose: To evaluate histopathological and ultrastructural changes and expression of proteins related to apoptosis CASPASE 3 and XIAP after experimental induction of temporary focal cerebral ischemia (90 minutes) due to obstruction of the middle cerebral artery in alcoholism model. Methods: Forty adult Wistar rats were used, subdivided into 5 experimental groups: control group (C); Sham group (S); Ischemic group (I); Alcoholic group (A); and Ischemic and Alcoholized group (I+A): animals submitted to the same treatment of group A and after four weeks were submitted to focal cerebral ischemia during 90 minutes, followed by reperfusion of 48 hours. Were processed for histopathological analysis and immunohistochemistry (for the protein expression of CASPASE -3 and XIAP). Results: Greater histopathological changes were observed in the animals of groups I and I+A in the three areas analyzed. The neuronal loss was higher in the medial striatum region of the animals of groups I and I + A. The protein expression of CASPASE -3 was higher than that of XIAP in the groups I and I + A for both proteins. Conclusion: The expression of XIAP was slightly higher where the histopathological changes and expression of CASPASE -3 was less evident.
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spelling Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholismBrain IschemiaApoptosisAlcoholismMorphologyRatsAbstract Purpose: To evaluate histopathological and ultrastructural changes and expression of proteins related to apoptosis CASPASE 3 and XIAP after experimental induction of temporary focal cerebral ischemia (90 minutes) due to obstruction of the middle cerebral artery in alcoholism model. Methods: Forty adult Wistar rats were used, subdivided into 5 experimental groups: control group (C); Sham group (S); Ischemic group (I); Alcoholic group (A); and Ischemic and Alcoholized group (I+A): animals submitted to the same treatment of group A and after four weeks were submitted to focal cerebral ischemia during 90 minutes, followed by reperfusion of 48 hours. Were processed for histopathological analysis and immunohistochemistry (for the protein expression of CASPASE -3 and XIAP). Results: Greater histopathological changes were observed in the animals of groups I and I+A in the three areas analyzed. The neuronal loss was higher in the medial striatum region of the animals of groups I and I + A. The protein expression of CASPASE -3 was higher than that of XIAP in the groups I and I + A for both proteins. Conclusion: The expression of XIAP was slightly higher where the histopathological changes and expression of CASPASE -3 was less evident.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2018-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000800652Acta Cirúrgica Brasileira v.33 n.8 2018reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/s0102-865020180080000001info:eu-repo/semantics/openAccessSchiavoni,Vagner SarraipoSilva,Jairo Pinheiro daLizarte Neto,Fermino SanchesAssis,Múcio Luiz Cirino deTazima,Maria de Fátima Galli SoritaCarvalho,Camila Albuquerque Melo deTirapelli,Daniela Pretti da CunhaCarlotti Jr,Carlos GilbertoColli,Benedicto OscarTirapelli,Luis Fernandoeng2018-09-03T00:00:00Zoai:scielo:S0102-86502018000800652Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2018-09-03T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism
title Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism
spellingShingle Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism
Schiavoni,Vagner Sarraipo
Brain Ischemia
Apoptosis
Alcoholism
Morphology
Rats
title_short Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism
title_full Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism
title_fullStr Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism
title_full_unstemmed Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism
title_sort Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism
author Schiavoni,Vagner Sarraipo
author_facet Schiavoni,Vagner Sarraipo
Silva,Jairo Pinheiro da
Lizarte Neto,Fermino Sanches
Assis,Múcio Luiz Cirino de
Tazima,Maria de Fátima Galli Sorita
Carvalho,Camila Albuquerque Melo de
Tirapelli,Daniela Pretti da Cunha
Carlotti Jr,Carlos Gilberto
Colli,Benedicto Oscar
Tirapelli,Luis Fernando
author_role author
author2 Silva,Jairo Pinheiro da
Lizarte Neto,Fermino Sanches
Assis,Múcio Luiz Cirino de
Tazima,Maria de Fátima Galli Sorita
Carvalho,Camila Albuquerque Melo de
Tirapelli,Daniela Pretti da Cunha
Carlotti Jr,Carlos Gilberto
Colli,Benedicto Oscar
Tirapelli,Luis Fernando
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Schiavoni,Vagner Sarraipo
Silva,Jairo Pinheiro da
Lizarte Neto,Fermino Sanches
Assis,Múcio Luiz Cirino de
Tazima,Maria de Fátima Galli Sorita
Carvalho,Camila Albuquerque Melo de
Tirapelli,Daniela Pretti da Cunha
Carlotti Jr,Carlos Gilberto
Colli,Benedicto Oscar
Tirapelli,Luis Fernando
dc.subject.por.fl_str_mv Brain Ischemia
Apoptosis
Alcoholism
Morphology
Rats
topic Brain Ischemia
Apoptosis
Alcoholism
Morphology
Rats
description Abstract Purpose: To evaluate histopathological and ultrastructural changes and expression of proteins related to apoptosis CASPASE 3 and XIAP after experimental induction of temporary focal cerebral ischemia (90 minutes) due to obstruction of the middle cerebral artery in alcoholism model. Methods: Forty adult Wistar rats were used, subdivided into 5 experimental groups: control group (C); Sham group (S); Ischemic group (I); Alcoholic group (A); and Ischemic and Alcoholized group (I+A): animals submitted to the same treatment of group A and after four weeks were submitted to focal cerebral ischemia during 90 minutes, followed by reperfusion of 48 hours. Were processed for histopathological analysis and immunohistochemistry (for the protein expression of CASPASE -3 and XIAP). Results: Greater histopathological changes were observed in the animals of groups I and I+A in the three areas analyzed. The neuronal loss was higher in the medial striatum region of the animals of groups I and I + A. The protein expression of CASPASE -3 was higher than that of XIAP in the groups I and I + A for both proteins. Conclusion: The expression of XIAP was slightly higher where the histopathological changes and expression of CASPASE -3 was less evident.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000800652
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000800652
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s0102-865020180080000001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.33 n.8 2018
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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