Expression of HSP70 in cerebral ischemia and neuroprotetive action of hypothermia and ketoprofen
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos de neuro-psiquiatria (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2010000400021 |
Resumo: | Heat shock proteins (HSPs) are molecular chaperones that bind to other proteins to shepherd them across membranes and direct them to specific locations within a cell. Several injurious stimuli can induce Hsp70 expression, including ischemia. This study aimed to investigate the pattern of expression of protein (immunohistochemistry) and gene (real-time PCR) Hsp70 in experimental focal cerebral ischemia in rats by occlusion of the middle cerebral artery for 1 hour and the role of neuroprotection with hypothermia (H) and ketoprofen (K). The infarct volume was measured using morphometric analysis defined by triphenyl tetrazolium chloride. It was observed increases in the protein (p=0.0001) and gene (p=0.0001) Hsp70 receptor in the ischemic areas that were reduced by H (protein and gene: p<0.05), K (protein: p<0.001), and H+K (protein: p<0.01 and gene: p<0.05). The Hsp70 increases in the ischemic area suggests that the Hsp70-mediated neuroexcitotoxicity plays an important role in cell death and that the neuroprotective effect of both, H and K are directly involved with the Hsp70. |
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Expression of HSP70 in cerebral ischemia and neuroprotetive action of hypothermia and ketoprofenHsp70 expressioncerebral ischemianeuroprotectionhypothermiaketoprofenHeat shock proteins (HSPs) are molecular chaperones that bind to other proteins to shepherd them across membranes and direct them to specific locations within a cell. Several injurious stimuli can induce Hsp70 expression, including ischemia. This study aimed to investigate the pattern of expression of protein (immunohistochemistry) and gene (real-time PCR) Hsp70 in experimental focal cerebral ischemia in rats by occlusion of the middle cerebral artery for 1 hour and the role of neuroprotection with hypothermia (H) and ketoprofen (K). The infarct volume was measured using morphometric analysis defined by triphenyl tetrazolium chloride. It was observed increases in the protein (p=0.0001) and gene (p=0.0001) Hsp70 receptor in the ischemic areas that were reduced by H (protein and gene: p<0.05), K (protein: p<0.001), and H+K (protein: p<0.01 and gene: p<0.05). The Hsp70 increases in the ischemic area suggests that the Hsp70-mediated neuroexcitotoxicity plays an important role in cell death and that the neuroprotective effect of both, H and K are directly involved with the Hsp70.Academia Brasileira de Neurologia - ABNEURO2010-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2010000400021Arquivos de Neuro-Psiquiatria v.68 n.4 2010reponame:Arquivos de neuro-psiquiatria (Online)instname:Academia Brasileira de Neurologiainstacron:ABNEURO10.1590/S0004-282X2010000400021info:eu-repo/semantics/openAccessTirapelli,Daniela Pretti da CunhaCarlotti Junior,Carlos GilbertoLeite,João PereiraTirapelli,Luis FernandoColli,Benedicto Oscareng2010-08-11T00:00:00Zoai:scielo:S0004-282X2010000400021Revistahttp://www.scielo.br/anphttps://old.scielo.br/oai/scielo-oai.php||revista.arquivos@abneuro.org1678-42270004-282Xopendoar:2010-08-11T00:00Arquivos de neuro-psiquiatria (Online) - Academia Brasileira de Neurologiafalse |
dc.title.none.fl_str_mv |
Expression of HSP70 in cerebral ischemia and neuroprotetive action of hypothermia and ketoprofen |
title |
Expression of HSP70 in cerebral ischemia and neuroprotetive action of hypothermia and ketoprofen |
spellingShingle |
Expression of HSP70 in cerebral ischemia and neuroprotetive action of hypothermia and ketoprofen Tirapelli,Daniela Pretti da Cunha Hsp70 expression cerebral ischemia neuroprotection hypothermia ketoprofen |
title_short |
Expression of HSP70 in cerebral ischemia and neuroprotetive action of hypothermia and ketoprofen |
title_full |
Expression of HSP70 in cerebral ischemia and neuroprotetive action of hypothermia and ketoprofen |
title_fullStr |
Expression of HSP70 in cerebral ischemia and neuroprotetive action of hypothermia and ketoprofen |
title_full_unstemmed |
Expression of HSP70 in cerebral ischemia and neuroprotetive action of hypothermia and ketoprofen |
title_sort |
Expression of HSP70 in cerebral ischemia and neuroprotetive action of hypothermia and ketoprofen |
author |
Tirapelli,Daniela Pretti da Cunha |
author_facet |
Tirapelli,Daniela Pretti da Cunha Carlotti Junior,Carlos Gilberto Leite,João Pereira Tirapelli,Luis Fernando Colli,Benedicto Oscar |
author_role |
author |
author2 |
Carlotti Junior,Carlos Gilberto Leite,João Pereira Tirapelli,Luis Fernando Colli,Benedicto Oscar |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Tirapelli,Daniela Pretti da Cunha Carlotti Junior,Carlos Gilberto Leite,João Pereira Tirapelli,Luis Fernando Colli,Benedicto Oscar |
dc.subject.por.fl_str_mv |
Hsp70 expression cerebral ischemia neuroprotection hypothermia ketoprofen |
topic |
Hsp70 expression cerebral ischemia neuroprotection hypothermia ketoprofen |
description |
Heat shock proteins (HSPs) are molecular chaperones that bind to other proteins to shepherd them across membranes and direct them to specific locations within a cell. Several injurious stimuli can induce Hsp70 expression, including ischemia. This study aimed to investigate the pattern of expression of protein (immunohistochemistry) and gene (real-time PCR) Hsp70 in experimental focal cerebral ischemia in rats by occlusion of the middle cerebral artery for 1 hour and the role of neuroprotection with hypothermia (H) and ketoprofen (K). The infarct volume was measured using morphometric analysis defined by triphenyl tetrazolium chloride. It was observed increases in the protein (p=0.0001) and gene (p=0.0001) Hsp70 receptor in the ischemic areas that were reduced by H (protein and gene: p<0.05), K (protein: p<0.001), and H+K (protein: p<0.01 and gene: p<0.05). The Hsp70 increases in the ischemic area suggests that the Hsp70-mediated neuroexcitotoxicity plays an important role in cell death and that the neuroprotective effect of both, H and K are directly involved with the Hsp70. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2010000400021 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2010000400021 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0004-282X2010000400021 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Neurologia - ABNEURO |
publisher.none.fl_str_mv |
Academia Brasileira de Neurologia - ABNEURO |
dc.source.none.fl_str_mv |
Arquivos de Neuro-Psiquiatria v.68 n.4 2010 reponame:Arquivos de neuro-psiquiatria (Online) instname:Academia Brasileira de Neurologia instacron:ABNEURO |
instname_str |
Academia Brasileira de Neurologia |
instacron_str |
ABNEURO |
institution |
ABNEURO |
reponame_str |
Arquivos de neuro-psiquiatria (Online) |
collection |
Arquivos de neuro-psiquiatria (Online) |
repository.name.fl_str_mv |
Arquivos de neuro-psiquiatria (Online) - Academia Brasileira de Neurologia |
repository.mail.fl_str_mv |
||revista.arquivos@abneuro.org |
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1754212769087881216 |