Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/196771 |
Resumo: | This study aimed to investigate the morphometric and the pattern of protein and gene expression related to the extrinsic apoptotic pathway in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. For this analysis, 120 rats were randomly divided into 3 groups (20 animals each): control - no surgery (20 animals); sham - simulation of surgery (20 animals); ischemic - focal ischemia for 1 hour, without reperfusion (80 animals) and divided into four subgroups with 20 animals each: ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic + hypothermia and ketoprofen. The infarct volume was measured using morphometric analysis of infarct areas defined by triphenyl tetrazolium chloride and the patterns of expression of the apoptosis genes (Fas, c-Flip, caspase-8 and caspase-3) and the apoptosis protein caspase-3 were evaluated by quantitative real-time PCR and immunohistochemistry, respectively. Hypo expression of genes of extrinsic pathway of apoptosis was observed: Fas receptor, c-Flip and caspase-8 in the isehemics areas. Increases in the gene and protein caspase-3 in the ischemic areas were also observed, and these increases were reduced by hypothermia and ketoprofen, also noted in the morphometric study. The caspases-3 increase suggests that this gene plays an important role in apoptosis, probably culminating in cell death and that the neuroprotective effect of hypothennia and ketoprofen is involved. |
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Repositório Institucional da UNESP |
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Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and KetoprofenApoptosisGene expressionCaspase-3Cerebral ischemiaNeuroprotectionHypothermiaKetoprofenThis study aimed to investigate the morphometric and the pattern of protein and gene expression related to the extrinsic apoptotic pathway in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. For this analysis, 120 rats were randomly divided into 3 groups (20 animals each): control - no surgery (20 animals); sham - simulation of surgery (20 animals); ischemic - focal ischemia for 1 hour, without reperfusion (80 animals) and divided into four subgroups with 20 animals each: ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic + hypothermia and ketoprofen. The infarct volume was measured using morphometric analysis of infarct areas defined by triphenyl tetrazolium chloride and the patterns of expression of the apoptosis genes (Fas, c-Flip, caspase-8 and caspase-3) and the apoptosis protein caspase-3 were evaluated by quantitative real-time PCR and immunohistochemistry, respectively. Hypo expression of genes of extrinsic pathway of apoptosis was observed: Fas receptor, c-Flip and caspase-8 in the isehemics areas. Increases in the gene and protein caspase-3 in the ischemic areas were also observed, and these increases were reduced by hypothermia and ketoprofen, also noted in the morphometric study. The caspases-3 increase suggests that this gene plays an important role in apoptosis, probably culminating in cell death and that the neuroprotective effect of hypothennia and ketoprofen is involved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FMRP USP, Dept Surg & Anat, Surg Clin Program, Ribeirao Preto, SP, BrazilUNESP, Dept Anat, Botucatu, SP, BrazilUniv Marilia, Fac Odontol, Marilia, SP, BrazilUNESP, Dept Anat, Botucatu, SP, BrazilFAPESP: 2005/00381-8Soc Chilena AnatomiaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Univ MariliaTirapelli, Daniela P. C.Assis Cirino, Mucio Luiz deMelo de Carvalho, Camila A.Novais, Paulo CezarFigueredo, Rafael ZimakPorsani, Lucas BarbosaGula, Isabella StracieriRodrigues, Andressa RomualdoSilva, Jairo Pinheiro daPereira, Adriana LisLizarte Neto, Fermino SanchesSchimming, Bruno Cesar [UNESP]Galli Sorita Tazima, Maria de FatimaSassoli Fazan, Valeria de PaulaCarlotti Jr, Carlos GilbertoTirapelli, Luis FernandoColli, Benedicto Oscar2020-12-10T19:55:41Z2020-12-10T19:55:41Z2020-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article523-529International Journal Of Morphology. Temuco: Soc Chilena Anatomia, v. 38, n. 3, p. 523-529, 2020.0717-9502http://hdl.handle.net/11449/196771WOS:000525755300001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal Of Morphologyinfo:eu-repo/semantics/openAccess2021-10-23T09:55:37Zoai:repositorio.unesp.br:11449/196771Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T09:55:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen |
title |
Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen |
spellingShingle |
Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen Tirapelli, Daniela P. C. Apoptosis Gene expression Caspase-3 Cerebral ischemia Neuroprotection Hypothermia Ketoprofen |
title_short |
Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen |
title_full |
Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen |
title_fullStr |
Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen |
title_full_unstemmed |
Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen |
title_sort |
Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen |
author |
Tirapelli, Daniela P. C. |
author_facet |
Tirapelli, Daniela P. C. Assis Cirino, Mucio Luiz de Melo de Carvalho, Camila A. Novais, Paulo Cezar Figueredo, Rafael Zimak Porsani, Lucas Barbosa Gula, Isabella Stracieri Rodrigues, Andressa Romualdo Silva, Jairo Pinheiro da Pereira, Adriana Lis Lizarte Neto, Fermino Sanches Schimming, Bruno Cesar [UNESP] Galli Sorita Tazima, Maria de Fatima Sassoli Fazan, Valeria de Paula Carlotti Jr, Carlos Gilberto Tirapelli, Luis Fernando Colli, Benedicto Oscar |
author_role |
author |
author2 |
Assis Cirino, Mucio Luiz de Melo de Carvalho, Camila A. Novais, Paulo Cezar Figueredo, Rafael Zimak Porsani, Lucas Barbosa Gula, Isabella Stracieri Rodrigues, Andressa Romualdo Silva, Jairo Pinheiro da Pereira, Adriana Lis Lizarte Neto, Fermino Sanches Schimming, Bruno Cesar [UNESP] Galli Sorita Tazima, Maria de Fatima Sassoli Fazan, Valeria de Paula Carlotti Jr, Carlos Gilberto Tirapelli, Luis Fernando Colli, Benedicto Oscar |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Univ Marilia |
dc.contributor.author.fl_str_mv |
Tirapelli, Daniela P. C. Assis Cirino, Mucio Luiz de Melo de Carvalho, Camila A. Novais, Paulo Cezar Figueredo, Rafael Zimak Porsani, Lucas Barbosa Gula, Isabella Stracieri Rodrigues, Andressa Romualdo Silva, Jairo Pinheiro da Pereira, Adriana Lis Lizarte Neto, Fermino Sanches Schimming, Bruno Cesar [UNESP] Galli Sorita Tazima, Maria de Fatima Sassoli Fazan, Valeria de Paula Carlotti Jr, Carlos Gilberto Tirapelli, Luis Fernando Colli, Benedicto Oscar |
dc.subject.por.fl_str_mv |
Apoptosis Gene expression Caspase-3 Cerebral ischemia Neuroprotection Hypothermia Ketoprofen |
topic |
Apoptosis Gene expression Caspase-3 Cerebral ischemia Neuroprotection Hypothermia Ketoprofen |
description |
This study aimed to investigate the morphometric and the pattern of protein and gene expression related to the extrinsic apoptotic pathway in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. For this analysis, 120 rats were randomly divided into 3 groups (20 animals each): control - no surgery (20 animals); sham - simulation of surgery (20 animals); ischemic - focal ischemia for 1 hour, without reperfusion (80 animals) and divided into four subgroups with 20 animals each: ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic + hypothermia and ketoprofen. The infarct volume was measured using morphometric analysis of infarct areas defined by triphenyl tetrazolium chloride and the patterns of expression of the apoptosis genes (Fas, c-Flip, caspase-8 and caspase-3) and the apoptosis protein caspase-3 were evaluated by quantitative real-time PCR and immunohistochemistry, respectively. Hypo expression of genes of extrinsic pathway of apoptosis was observed: Fas receptor, c-Flip and caspase-8 in the isehemics areas. Increases in the gene and protein caspase-3 in the ischemic areas were also observed, and these increases were reduced by hypothermia and ketoprofen, also noted in the morphometric study. The caspases-3 increase suggests that this gene plays an important role in apoptosis, probably culminating in cell death and that the neuroprotective effect of hypothennia and ketoprofen is involved. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T19:55:41Z 2020-12-10T19:55:41Z 2020-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
International Journal Of Morphology. Temuco: Soc Chilena Anatomia, v. 38, n. 3, p. 523-529, 2020. 0717-9502 http://hdl.handle.net/11449/196771 WOS:000525755300001 |
identifier_str_mv |
International Journal Of Morphology. Temuco: Soc Chilena Anatomia, v. 38, n. 3, p. 523-529, 2020. 0717-9502 WOS:000525755300001 |
url |
http://hdl.handle.net/11449/196771 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal Of Morphology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
523-529 |
dc.publisher.none.fl_str_mv |
Soc Chilena Anatomia |
publisher.none.fl_str_mv |
Soc Chilena Anatomia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964812052004864 |