Magnetic bead technology for viral RNA extraction from serum in blood bank screening
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Infectious Diseases |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000600008 |
Resumo: | Nucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of São Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. OBJECTIVE: Manual magnetic particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase - polymerase chain reaction (RT-PCR) one-step. METHODS: Blood donor samples were collected from January 2010 to September 2010, and minipools of them were submitted to testing. ELISA was used for the analysis of anti-HCV/HIV antibodies. Detection and amplification of viral RNA was performed using real-time PCR. RESULTS: Out of 20.808 samples screened, 53 samples (29 for HCV and 24 for HIV) were confirmed as positive by serological and NAT methods. CONCLUSION: The manual magnetic bead-based extraction in combination with real-time PCR detection can be used to routinely screen blood donation for viremic donors to further increase the safety of blood products. |
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Brazilian Journal of Infectious Diseases |
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Magnetic bead technology for viral RNA extraction from serum in blood bank screeningHCVHIVinfectionreverse transcriptase polymerase chain reactionblood banksNucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of São Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. OBJECTIVE: Manual magnetic particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase - polymerase chain reaction (RT-PCR) one-step. METHODS: Blood donor samples were collected from January 2010 to September 2010, and minipools of them were submitted to testing. ELISA was used for the analysis of anti-HCV/HIV antibodies. Detection and amplification of viral RNA was performed using real-time PCR. RESULTS: Out of 20.808 samples screened, 53 samples (29 for HCV and 24 for HIV) were confirmed as positive by serological and NAT methods. CONCLUSION: The manual magnetic bead-based extraction in combination with real-time PCR detection can be used to routinely screen blood donation for viremic donors to further increase the safety of blood products.Brazilian Society of Infectious Diseases2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000600008Brazilian Journal of Infectious Diseases v.15 n.6 2011reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1590/S1413-86702011000600008info:eu-repo/semantics/openAccessAlbertoni,Guilherme AmbrozioArnoni,Carine PriscoAraujo,Patricia Regina BarbozaAndrade,Sheila SiqueiraCarvalho,Fabrício OliveiraGirão,Manoel João Batista CastelloSchor,NestorBarreto,José Augustoeng2012-01-04T00:00:00Zoai:scielo:S1413-86702011000600008Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2012-01-04T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false |
dc.title.none.fl_str_mv |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
title |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
spellingShingle |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening Albertoni,Guilherme Ambrozio HCV HIV infection reverse transcriptase polymerase chain reaction blood banks |
title_short |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
title_full |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
title_fullStr |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
title_full_unstemmed |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
title_sort |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
author |
Albertoni,Guilherme Ambrozio |
author_facet |
Albertoni,Guilherme Ambrozio Arnoni,Carine Prisco Araujo,Patricia Regina Barboza Andrade,Sheila Siqueira Carvalho,Fabrício Oliveira Girão,Manoel João Batista Castello Schor,Nestor Barreto,José Augusto |
author_role |
author |
author2 |
Arnoni,Carine Prisco Araujo,Patricia Regina Barboza Andrade,Sheila Siqueira Carvalho,Fabrício Oliveira Girão,Manoel João Batista Castello Schor,Nestor Barreto,José Augusto |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Albertoni,Guilherme Ambrozio Arnoni,Carine Prisco Araujo,Patricia Regina Barboza Andrade,Sheila Siqueira Carvalho,Fabrício Oliveira Girão,Manoel João Batista Castello Schor,Nestor Barreto,José Augusto |
dc.subject.por.fl_str_mv |
HCV HIV infection reverse transcriptase polymerase chain reaction blood banks |
topic |
HCV HIV infection reverse transcriptase polymerase chain reaction blood banks |
description |
Nucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of São Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. OBJECTIVE: Manual magnetic particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase - polymerase chain reaction (RT-PCR) one-step. METHODS: Blood donor samples were collected from January 2010 to September 2010, and minipools of them were submitted to testing. ELISA was used for the analysis of anti-HCV/HIV antibodies. Detection and amplification of viral RNA was performed using real-time PCR. RESULTS: Out of 20.808 samples screened, 53 samples (29 for HCV and 24 for HIV) were confirmed as positive by serological and NAT methods. CONCLUSION: The manual magnetic bead-based extraction in combination with real-time PCR detection can be used to routinely screen blood donation for viremic donors to further increase the safety of blood products. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000600008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000600008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1413-86702011000600008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
dc.source.none.fl_str_mv |
Brazilian Journal of Infectious Diseases v.15 n.6 2011 reponame:Brazilian Journal of Infectious Diseases instname:Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
instname_str |
Brazilian Society of Infectious Diseases (BSID) |
instacron_str |
BSID |
institution |
BSID |
reponame_str |
Brazilian Journal of Infectious Diseases |
collection |
Brazilian Journal of Infectious Diseases |
repository.name.fl_str_mv |
Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID) |
repository.mail.fl_str_mv |
bjid@bjid.org.br||lgoldani@ufrgs.br |
_version_ |
1754209241969721344 |